Survival under space vacuum — Biochemical aspects

Exposure to vacuum predominantly causes the removal of water. As a consequence hydrophobic bonds (e.g. of membranes and proteins) are disrupted and metabolism practically comes to a complete halt. Removal of hydrate water also causes substantial changes regarding the structure of DNA (A-structure likely prevails). Some organisms, however, especially bacterial spores and fungal conidia are so well adapted to extreme dryness that substantial fractions of these organisms survive several months of vacuum even at room temperature. In these organisms some vacuum-induced alterations occur that are not readily reversed by readdition of water; mutations become evident and the amount of DNA covalently bound to protein is drastically increased. The mechanisms of these processes and their possible repair are not yet clear. There is evidence that chemical reactions (e.g. dehydration reactions) are involved although they likely proceed at an extremely low rate. Using the dehydration of serin by vacuum as a model system (the resulting amino acrylic acid is converted into pyruvic acid and ammonia after reexposure to water) we could establish that about 3 out of 100 000 serins are finally converted into pyruvic acid after exposure to 10⁻⁶ Torr for 1 week at 55°C. In dry Ar the corresponding rate is only about 1.5.     

Electron beam experiments in Japanese satellites and rockets

Electron beam experiments in space that have been done and planned in Japan are reviewed. 200eV, 1mA electron beam is emitted from a satellite and several types of wave excitation such as UHF and ω_ce have been observed. The satellite potential and the energy spectrum of returning electrons are measured by Langmuir probes and electrostatic energy analyser. In rocket experiments of K-10-11, K-10-12, K-9M-57, K-9M-58, K-9M-61 and K-9M-66, several types of electron guns were used whose power ranges from 1mW to 1KW. The rocket potential was measured by Langmuir probes and floating probes and optical line emission measurement and wave measurements were also done. The rocket potential was not so high as expected from the balance with ionospheric plasma but strongly affected by the plasma production by the emitted electron beam and return electrons.     

FIRST — Far Infrared and Submillimetre Space Telescope

The present status of the ESA cornerstone mission FIRST is presented. A recent industrial study has generated a spacecraft concept employing a 4.5 m passively cooled telescope with focal plane instrument cooling provided by a superfluid helium cryostat. The model payload complement includes two direct detection instruments as well as two heterodyne instruments. After a shared launch by Ariane 5 into GTO, FIRST propels itself into the 24-hour highly eccentric operational orbit, where observations can be conducted up to 17 hours per day with an expected approximate mission duration of 3 years. An additional complementary study of a non-cryostat spacecraft option will also be performed.     

Enzyme—Mimicking properties of silicates and other minerals

The adsorptive and/or catalytic properties of clays, silicates in general, and other minerals are well known. More recently, their probable role in prebiotic syntheses of bio-organic compounds has become a matter of record. We demonstrate that, in addition to their role in $\text{de novo}$ formation of important biomolecules, clays, micas, fibrous silicates and other minerals mimick the activities of contemporary enzymes including oxidases, esterases, phosphatases and glucosidases. The existence of such capabilities in substances likely to be represented on the surfaces of Earth-like planets may offer a challenge to the technology and design of remote life detection systems which must then distinguish between bona fide biological chemistry and mineral-base pseudometabolism. It also raises questions about the importance of mineral surfaces in post-mortem transformations of organic metabolites in our own biosphere.     

Submillimeter spectroscopy from space — Missions planned between 1995 and 2005

The submillimeter wavelength range (100 μm ≤ λ ≤ 1 mm) is exceptionally rich in atomic and molecular transitions that provide direct measures of the chemical composition, temperature, density, and cooling of the interstellar medium. In part, this is due to the fact that the most abundant oxygen- and carbon-bearing species as well as the most common hydrides have their lowest lying transitions within this wavelength region. However, because of the great abundance of many of these same species in our own atmosphere (e.g., H₂O and O₂), terrestrial absorption at submillimeter wavelengths is strong, rendering much of the sky inaccessible to ground- and even airborne observing platforms. In recognition of the scientific importance of the submillimeter range to astronomy and the limitations placed on its pursuit by the atmosphere, both NASA and ESA have embarked on ambitious programs to explore these wavelengths from space.     

Cyclotron radiation of magnetized degenerate dwarfs and neutron stars: II—Observations

A brief review of observations is given of cyclotron features of radiation from degenerated stars. Explanations of these data and predictions of new observational effects are proposed on the basis of theoretical results presented in Paper I.     

Climate studies from satellite observations: Special problems in the verification of earth radiation balance,cloud climatology,and related climate experiments

A body of techniques that have been developed and planned for use during the Earth Radiation Budget Experiment (ERBE), the International Satellite Cloud Climatology Project (ISCCP), and related climate experiments of the 1980's are reviewed. Validation and verification methods must apply for $\text{systems}$ of satellites. They include: (1) use of a normalization or intercalibration satellite, (2) special intensive observation areas located over ground-truth sites, and (3) monitoring of sun and earth by several satellites and/or several instruments at the same time. Since each climate application area has a hierarchy of user communities, validation techniques vary from very detailed methods to those that simply assure high relative accuracy in detecting space and time variations for climate studies. It is shown that climate experiments generally require more emphasis on long-term stability and internal consistency of satellite data sets than high absolute accuracy.     

The Japanese lunar mission SELENE: Science goals and present status

The Japanese lunar mission SELENE (SELenological and ENgineering Explorer) has been in development to target launch scheduled 2007 summer by H-IIA rocket. The SELENE is starting final integration test after SAR (System Acceptance Review), SRR (System Reliability Review) and instrument environment test. The SELENE is a remote-sensing mission orbiting 100 km altitude of the Moon for nominal one year and extended some months to collect the data for studying the origin and evolution of the Moon. Fourteen instruments and experiment systems are preparing for studies of the Moon, in the Moon, and from the Moon; global element and mineral compositions, topological structure, gravity field of whole moon, and electromagnetic and particle environment of the Moon. The new data center SOAC (SELENE Operation and data Analysis Center) are completed to construct in JAXA Sagamihara campus, and end-to-end test will be carried out between SOAC and data downlink stations.     

Heterogeneous solid/gas chemistry of organic compounds related to comets,meteorites, Titan,and Mars: Laboratory and in lower Earth orbit experiments

To understand the evolution of organic molecules involved in extraterrestrial environments and with exobiological implications, many experimental programs in the laboratory are devoted to photochemical studies in the gaseous phase as well as in the solid state. The validity of such studies and their applications to extraterrestrial environments can be questioned as long as experiments conducted in space conditions, with the full solar spectrum, especially in the short wavelength domain, have not been implemented. The experiments that are described here will be carried out on a FOTON capsule, using the BIOPAN facility, and on the International Space Station, using the EXPOSE facility. Vented and sealed exposition cells will be used, which will allow us to study the chemical evolution in the gaseous phase as well as heterogeneous processes, such as the degradation of solid compounds and the release of gaseous fragments.     

Meteorites in Antarctica — Statistics on falls, concentration, recovery and alteration on ice-sheet

The Antarctic meteorites are distributed on the blue-ice area surfaces in the ablation zone of the Antarctic ice-sheet, to where meteorites have been transported by the ice-flow within the ice-sheet from the wider accumulation zone. Among the Antarctic meteorite collection H- and L-chondrites are most abundant; this is also true in the non-Antarctic meteorite collection. Meteorite showers also are involved in the collection. Several new types of stony meteorites have been discovered from the Antarctic meteorite collection. The mass and shape of Antarctic meteorites are in agreement with those of resultant fragments of high-speed impact basaltic rocks. In Antarctica, small fragments of meteorite smaller than 1 kg in weight can easily be found and collected. The solidification and the gas retention ages of Antarctic meteorites also are concentrated around 4.5×10⁹ years, but some of them are considerably younger. Their cosmic-ray exposure ages are extended up to 9×10⁶ years and their terrestrial ages are 9×10⁴-7×10⁵ years.     

The ORGANIC experiment on EXPOSE-R on the ISS: Flight sample preparation and ground control spectroscopy

In March of 2009, the ORGANIC experiment integrated into the European multi-user facility EXPOSE-R, containing experiments dedicated to Astrobiology, was mounted through Extra Vehicular Activity (EVA) externally on the International Space Station (ISS). The experiment exposed organic samples of astronomical interest for a duration of 97 weeks (∼22 months) to the space environment. The samples that were returned to Earth in spring 2011, received a total UV radiation dose during their exposure including direct solar irradiation of >2500 h, exceeding the limits of laboratory simulations. We report flight sample preparation and pre-flight ultraviolet–visible (UV–Vis) characterization of the ORGANIC samples, which include 11 polycyclic aromatic hydrocarbons (PAHs) and three fullerenes. The corresponding time-dependent ground control monitoring experiments for ORGANIC measured over ∼19 months are presented and the results anticipated upon return of the samples are discussed. We present the first UV–Vis spectrum of solid circobiphenyl (C₃₈H₁₆). Further, we present the first published UV–Vis spectra of diphenanthro[9,10-b′,10′-d]thiophene (C₂₈H₁₆S), dinaphtho[8,1,2-abc,2′,1′,8′-klm]coronene (C₃₆H₁₆), tetrabenzo[de,no,st,c′d′]heptacene (C₄₂H₂₂), and dibenzo[jk,a′b′]octacene (C₄₀H₂₂) in solid phase and in solution. The results of the ORGANIC experiment are expected to enhance our knowledge of the evolution and degradation of large carbon-containing molecules in space environments.     

Auxin polar transport in Arabidopsis under simulated microgravity conditions — Relevance to growth and development

Activity of auxin polar transport in inflorescence axes of Arabidopsis thaliana grown under simulated microgravity conditions was studied in relation to the growth and development. Seeds were germinated and allowed to grow on an agar medium in test tubes on a horizontal clinostat. Horizontal clinostat rotation substantially reduced the growth of inflorescence axes and the productivity of seeds of Arabidopsis thaliana (ecotypes Landsberg erecta and Columbia), although it little affected seed germination, development of rosette leaves and flowering. The activity of auxin polar transport in inflorescence axes decreased when Arabidopsis plants were grown on a horizontal clinostat from germination stage, being ca. 60% of 1 g control. On the other hand, the auxin polar transport in inflorescence axes of Arabidopsis grown in 1 g conditions was not affected when the segments were exposed to various gravistimuli, including 3-dimensional clinorotation, during transport experiments. Pin-formed mutant of Arabidopsis, having a unique structure of the inflorescence axis with no flower and extremely low levels of the activity of auxin polar transport in inflorescence axes and endogenous auxin, did not continue its vegetative growth under clinostat rotation. These facts suggest that the development of the system of auxin polar transport in Arabidopsis is affected by microgravity, resulting in the inhibition of growth and development, especially during reproductive growth.     

Global N2O cycles — Terrestrial emissions, atmospheric accumulation and biospheric effects

Tropospheric nitrous oxide concentration has increased by 0.2 – 0.4% per year over the period 1975 to 1982, amounting to net addition to the atmosphere of 2.8 – 5.6 Tg N₂O-N per year. This perturbation, if continued into the future, will affect stratospheric chemical cycles, and the thermal balance of the Earth. In turn it will have direct and indirect global effects on the biosphere. Though the budget and cycles of N₂O on Earth are not yet fully resolved, accumulating information and recent modelling efforts enable a more complete evaluation and better definition of gaps in our knowledge.     

The effects of proton radiation on UHMWPE material properties for space flight and medical applications

Ultra High Molecular Weight Polyethylene (UHMWPE) is a polymer widely used as a radiation shielding material in space flight applications and as a bearing material in total joint replacements. As a long chain hydrocarbon based polymer, UHMWPE’s material properties are influenced by radiation exposure, and prior studies show that gamma irradiation is effective for both medical sterilization and increased wear resistance in total joint replacement applications. However, the effects of space flight radiation types and doses on UHMWPE material properties are poorly understood. In this study, three clinically relevant grades of UHMWPE (GUR 1020, GUR 1050, and GUR 1020 blended with Vitamin E) were proton irradiated and tested for differences in material properties. Each of the three types of UHMWPE was irradiated at nominal doses of 0 Gy (control), 5 Gy, 10 Gy, 20 Gy, and 35 Gy. Following irradiation, uniaxial tensile testing and thermal testing using Differential Scanning Calorimetry (DSC) and Dynamic Mechanical Analysis (DMA) were performed. Results show small but significant changes in several material properties between the control (0 Gy) and 35 Gy samples, indicating that proton irradiation could have a effect on the long term performance of UHMWPE in both medical and space flight applications.     

The role of hydration and radiation quality in the induction of DNA damage--chemical aspects.

The mutagenic and lethal effects of ionising radiation are thought to result from chemical modifications induced within DNA. This DNA damage is significantly influenced by the chemical environment and the radiation quality (LET). Water closely associated with the DNA and its immediate environment is involved in the early chemical pathways which lead to the induction of DNA damage and is reflected in the cellular radiosensitivity. For instance, hydration of DNA influences hole migration leading to its localisation at guanine. Changes in the radiation quality are discussed in terms of the complexity of the radical clusters produced. It is inferred that at higher LET, the influence of the chemical environment (O2 etc) decreases with respect to DNA damage and cellular radiosensitivity. It is therefore important to include these effects of environment of the DNA upon the early chemical pathways in models of radiation action.     

Electron beams and their interactions with the ionosphere — A review of the E| |B series

A review is presented of results from a series of experiments which began with an attempt to reflect electrons from the potential change which is thought to generate the aurora. In order to understand the experiment, this series then expanded to study beam-plasma interactions in the laboratory as well as in the ionosphere. Results from the electron reflection experiment, as well as in situ and ground-based observations of the waves generated by beam-plasma interactions will be reviewed. Measurements bearing on modifications of the ambient plasma by the beam-emitting rocket will be presented.     

Present results of the joint radiobiological ESA/IBMP experiments “seeds” aboard Cosmos 2044 and 2229 - correlation of micro-dosimetric data and damage endpoints in Arabidopsis thaliana plants

Experimental data obtained from two Cosmos missions (2044, 2229) performed with dry plant seeds of Arabidopsis thaliana in a Biostack configuration are compared. Biological stress and genetic risk are induced in dry seeds by cosmic radiation, especially the high energetic, densely ionizing component of eavy ons (HI). Subdivision of impacting HI particles into LET-groups (15–35; 35–100; >100 keV/μm) showed the contribution of each LET group to the induction of different biological damage endpoints (survival, cell transformation, lethal plant development). An attempt is presented to comprehend the influence of the spatial energy deposition as a biophysical HI track parameter.