Chromosome aberrations in human lymphocytes induced by 250 MeV protons: effects of dose, dose rate and shielding.

Although the space radiation environment consists predominantly of energetic protons, astronauts inside a spacecraft are chronically exposed to both primary particles as well as secondary particles that are generated when the primary particles penetrate the spacecraft shielding. Secondary neutrons and secondary charged particles can have an LET value that is greater than the primary protons and, therefore, produce a higher relative biological effectiveness (RBE). Using the accelerator facility at Loma Linda University, we exposed human lymphocytes in vitro to 250 MeV protons with doses ranging from 0 to 60 cGy at three different dose rates: a low dose rate of 7.5 cGy/h, an intermediate dose rate of 30 cGy/h and a high dose rate of 70 cGy/min. The effect of 15 g/cm2 aluminum shielding on the induction of chromosome aberrations was investigated for each dose rate. After exposure, lymphocytes were incubated in growth medium containing phytohemagglutinin (PHA) and chromosome spreads were collected using a chemical-induced premature chromosome condensation (PCC) technique. Aberrations were analyzed using the fluorescence in situ hybridization (FISH) technique with three different colored chromosome-painting probes. The frequency of reciprocal and complex-type chromosome exchanges were compared in shielded and unshielded samples.     

Non-stationarity and cross-correlation effects in the MHD solar activity

The analysis of turbulent processes in sunspots and pores which are self-organizing long-lived magnetic structures is a complicated and not yet solved problem. The present work focuses on studying such magneto-hydrodynamic (MHD) formations on the basis of flicker-noise spectroscopy using a new method of multi-parametric analysis. The non-stationarity and cross-correlation effects taking place in solar activity dynamics are considered. The calculated maximum values of non-stationarity factor may become precursors of significant restructuring in solar magnetic activity. The introduced cross-correlation functions enable us to judge synchronization effects between the signals of various solar activity indicators registered simultaneously.     

A follow-up of in-orbit observations of radiation-induced effects in commercial of the shelf memories on-board Alsat-1

This paper presents a follow-up of the results of an 8-year study on radiation effects in commercial off the shelf (COTS) memory devices operating within the on-board data handling system of the Algerian micro-satellite Alsat-1 in a Low-Earth Orbit (LEO). A statistical analysis of single-event upset (SEU) and multiple-bit upset (MBU) activity in commercial memories on-board the Alsat-1 primary On-Board Computer (OBC-386) is given. The OBC-386 is an Intel 80C386EX based system that plays a dual role for Alsat-1, acting as the key component of the payload computer as well as the command and control computer for the micro-satellite. The in-orbit observations show that the typical SEU rate at Alsat-1’s orbit is 4.04 × 10⁻⁷ SEU/bit/day, where 98.6% of these SEUs cause single-bit errors, 1.22% cause double-byte errors, and the remaining SEUs result in multiple-bit and severe errors.     

Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa

Simulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa.     

Effects of dust polarity and nonextensive electrons on the dust-ion acoustic solitons and double layers in earth atmosphere

Propagation of dustion acoustic solitary waves (DIASWs) and double layers is discussed in earth atmosphere, using the Sagdeev potential method. The best model for distribution function of electrons in earth atmosphere is found by fitting available data on different distribution functions. The nonextensive function with parameter $q=0.58$ provides the best fit on observations. Thus we analyze the propagation of localized waves in an unmagnetized plasma containing nonextensive electrons, inertial ions, and negatively/positively charged stationary dust. It is found that both compressive and rarefactive solitons as well as double layers exist depending on the sign (and the value) of dust polarity. Characters of propagated waves are described using the presented model.     

Schumann resonance frequency and conductivity in the nighttime ionosphere of Mars: A source for lightning

We have solved the Maxwellian equations of electromagnetic waves which oscillate within the cavity formed in the lower ionosphere of Mars between 0 and 70?km. The electrical conductivity and Schumann Resonance (SR) frequencies are calculated in the lower ionosphere of Mars, in the presence of a major dust storm that occurred in Martian Year (MY) 25 at low latitude region (25°–35°S). It is found that the atmospheric conductivity reduced by one to two orders of magnitude in the presence of a dust storm. It represents a small dust layer at about 25–30?km altitudes where lightning can occur. We also found that the SR frequencies peak at?～18?km with values 19.9, 34.5 and 48.8?Hz for the modes l?=?1, 2 and 3, respectively, in the non-homogeneous medium. Our results indicate that practical or measurable values of SR are dependent on the altitudes.     

Excitation of flare-induced waves in coronal loops and the effects of radiative cooling

EUV imaging observations from several space missions (SOHO/EIT, TRACE, and SDO/AIA) have revealed a presence of propagating intensity disturbances in solar coronal loops. These disturbances are typically interpreted as slow magnetoacoustic waves. However, recent spectroscopic observations with Hinode/EIS of active region loops revealed that the propagating intensity disturbances are associated with intermittent plasma upflows (or jets) at the footpoints which are presumably generated by magnetic reconnection. For this reason, whether these disturbances are waves or periodic flows is still being studied. This study is aimed at understanding the physical properties of observed disturbances by investigating the excitation of waves by hot plasma injections from below and the evolution of flows and wave propagation along the loop. We expand our previous studies based on isothermal 3D MHD models of an active region to a more realistic model that includes full energy equation accounting for the effects of radiative losses. Computations are initialized with an equilibrium state of a model active region using potential (dipole) magnetic field, gravitationally stratified density and temperature obtained from the polytropic equation of state. We model an impulsive injection of hot plasma into the steady plasma outflow along the loops of different temperatures, warm (～1 MK) and hot (～6 MK). The simulations show that hot jets launched at the coronal base excite slow magnetoacoustic waves that propagate to high altitudes along the loops, while the injected hot flows decelerate rapidly with heights. Our results support that propagating disturbances observed in EUV are mainly the wave features. We also find that the effect of radiative cooling on the damping of slow-mode waves in 1–6 MK coronal loops is small, in agreement with the previous conclusion based on 1D MHD models.     

Patterns of carbon monoxide in the middle atmosphere and effects of solar variability

We determine the spatial-time patterns of zonally averaged carbon monoxide (CO) in the middle atmosphere by applying Principle Component Analysis to the CO data obtained from the Microwave Limb Sounder (MLS) measurements on the Aura satellite in 2004–2012. The first two principal components characterize more than 90% of the CO variability. Both principal components are localized in the low thermosphere near the mesopause. The first principal component is asymmetric relative to the poles. It has opposite signs in the Northern and Southern Hemisphere at mid to high latitudes and strongly oscillates with an annual periodicity. The second principal component has the same sign in both hemispheres and oscillates mainly with a semi-annual frequency. Both principal components are modulated by the 11-year solar cycle and display short-term variations. To test possible correlations of these variations with the short term solar ultraviolet (UV) variability we use the simultaneous measurements of the UV solar radiance from the Solar-Stellar Irradiance Comparison Experiment (SOLSTICE) on the Solar Radiation and Climate Experiment (SORCE) satellite to investigate the correlation between CO in the middle atmosphere and solar UV in 2004–2012. Using a wavelet coherence technique a weak, intermittent 27-day signal is detected in high-frequency parts of the CO principal components.     

Different space weather effects in anomalies of the high and low orbital satellites

Preliminary results of the EU INTAS Project 00810, which aims to improve the methods of safeguarding satellites in the Earth’s magnetosphere from the negative effects of the space environment, are presented. Anomaly data from the “Kosmos” series satellites in the period 1971–1999 are combined in one database, together with similar information on other spacecraft. This database contains, beyond the anomaly information, various characteristics of the space weather: geomagnetic activity indices (Ap, AE and Dst), fluxes and fluences of electrons and protons at different energies, high energy cosmic ray variations and other solar, interplanetary and solar wind data. A comparative analysis of the distribution of each of these parameters relative to satellite anomalies was carried out for the total number of anomalies (about 6000 events), and separately for high (5000 events) and low (about 800 events) altitude orbit satellites. No relation was found between low and high altitude satellite anomalies. Daily numbers of satellite anomalies, averaged by a superposed epoch method around sudden storm commencements and proton event onsets for high (>1500 km) and low (<1500 km) altitude orbits revealed a big difference in a behavior. Satellites were divided on several groups according to the orbital characteristics (altitude and inclination). The relation of satellite anomalies to the environmental parameters was found to be different for various orbits that should be taken into account under developing of the anomaly frequency models.     

Depth dependency of neutron density produced by cosmic rays in the lunar subsurface

Depth dependency of neutrons produced by cosmic rays (CRs) in the lunar subsurface was estimated using the three-dimensional Monte Carlo particle and heavy ion transport simulation code, PHITS, incorporating the latest high energy nuclear data, JENDL/HE-2007. The PHITS simulations of equilibrium neutron density profiles in the lunar subsurface were compared with the measurement by Apollo 17 Lunar Neutron Probe Experiment (LNPE). Our calculations reproduced the LNPE data except for the 350–400 mg/cm² region under the improved condition using the CR spectra model based on the latest observations, well-tested nuclear interaction models with systematic cross section data, and JENDL/HE-2007.     

光栅笔划叠加显示信号测试中的汉字显示

给出了在采用任意波形发生器、视频信号发生器等ATE通用仪器资源产生光栅笔划显示信号时 ,根据TTF字形轮廓数据产生字符笔划信号的方法 ,解决了ATE笔划光栅信号测试中汉字显示的问题。     

Estimation of the bias of the Minimum Variance technique in the determination of magnetic clouds global quantities and orientation

Magnetic clouds (MCs) are highly magnetized plasma structures that have a low proton temperature and a magnetic field vector that rotates when seen by a heliospheric observer. More than 25 years of observations of magnetic and plasma properties of MCs at 1 AU have provided significant knowledge of their magnetic structure. However, because in situ observations only give information along the trajectory of the spacecraft, their real 3D magnetic configuration remains still partially unknown. We generate a set of synthetic clouds, exploring the space of parameters that represents the possible orientations and minimum distances of the satellite trajectory to the cloud axis, p. The synthetic clouds have a local cylindrical symmetry and a linear force-free magnetic configuration. From the analysis of synthetic clouds, we quantify the errors introduced in the determination of the orientation/size (and, consequently, of the global magnetohydrodynamic quantities) by the Minimum Variance method when p is not zero.     

Protective effects of melatonin against C beam irradiation-induced oxidative stress and DNA injury in the mouse brain

The purpose of this experiment was to estimate the protective effects of melatonin against radiation-induced brain damages in mice induced by heavy ion beams. Kun-Ming mice were randomly divided into five groups: normal control group, irradiation control group, and three different doses of melatonin (5, 10, and 20 mg/kg, i.p.) treated groups. Apart from the normal control group, the other four groups were exposed to whole-body 4.0 Gy carbon ion beam irradiation (approximately 0.5 Gy/min) after i.p. administration of normal saline or melatonin 1 h before irradiation. The oxidative redox status of brain tissue was assessed by measurement of malondiadehyde (MDA) levels, total superoxide dismutase (T-SOD), cytosolic superoxide dismutase (Cu/ZnSOD, SOD1) and mitochondrial superoxide dismutase (MnSOD, SOD2) activities at 8 h after irradiation. DNA damages were determined using the Comet assay and apoptosis and cell cycle distribution were detected by flow cytometric analyses. A dramatic dose-dependent decrease in MDA levels, tail moment, rates of tailing cells, and apoptosis, and a dose-dependent increase in T-SOD and SOD2 activities, in brain tissues in the melatonin-treated groups were detected compared with the irradiation only group. Furthermore, flow cytometric analysis demonstrated that the percentage of brain cells in the G0/G1 phase decreased significantly, while those in the S and G2/M stage increased dramatically, with mice pretreated with melatonin compared to the irradiation control group. These data indicate that melatonin has protective effects against irradiation-induced brain injury, and that its underlying protective mechanisms may relate to modulation of oxidative stress induced by heavy ionirradiation.     

Advances in the Researches of the Middle and Upper Atmosphere in China in 2012–2014

In this report we summarize the research results by Chinese scientists in 2012–2014. The focuses are placed on the researches of the middle and upper atmosphere, specifically the researches related to ground-based observation capability development, dynamical processes, the property of circulation and chemistry-climate coupling of the middle atmospheric layers.     

Stability of the seasonal variations in diurnal and semidiurnal components of mid-latitude F2 layer parameters

We report work utilizing 15-min resolution ionospheric data obtained with DPS-4 digisonde in 2003–2011 to study the seasonal variations in amplitudes and phases of the most powerful spectral components of the F2 layer critical frequency (foF2) and peak height (hmF2) fluctuations over Irkutsk (52.5°N, 104.0°E). We show that fluctuations of both parameters contain quasi-harmonic components with periods of T_n = 24/n h (n = 1–7). The number of distinct spectral peaks varies from 3 in summer to 7 in winter. Amplitude and phase characteristics of the diurnal (n = 1) and semidiurnal (n = 2) components is studied using the data sets extracted from the original data sets with band-pass filter. It has been found that the amplitudes of diurnal/semidiurnal foF2 and diurnal hmF2 components are maximum in winter and minimum in summer. Amplitudes of the diurnal components vary gradually; those of the foF2 semidiurnal one, abruptly, thus forming a narrow winter maximum in November–January. The phase (local time of maximum) of the diurnal foF2 component increases gradually by 4–6 h from winter to summer. The phase of the semidiurnal foF2 component is nearly stable in winter/summer and sharply decreases (increases) by 2–3 h near the spring (autumn) equinox. The phase of the diurnal component of hmF2 (local time of minimum) varies slightly between 1130 and 1300 LT; that of the semidiurnal one decreases (increases) by 4–6 h from January to March (from September to November). The results obtained show that the main features of seasonal variations in the diurnal and semidiurnal components of the mid-latitude F2 layer parameters recur consistently during the solar activity growth and decline phases.     

Preliminary results of space experiment: Implications for the effects of space radiation and microgravity on survival and mutation induction in human cells

In view of the concern for the health of astronauts that may one day journey to Mars or the Moon, we investigated the effect that space radiation and microgravity might have on DNA damage and repair. We sent frozen human lymphoblastoid TK6 cells to the International Space Station where they were maintained under frozen conditions during a 134-day mission (14 November 2008 to 28 March 2009) except for an incubation period of 8 days under 1G or μG conditions in a CO₂ incubator. The incubation period started after 100 days during which the cells had been exposed to 54 mSv of space radiation. The incubated cells were then refrozen, returned to Earth, and compared to ground control samples for the determination of the influence of microgravity on cell survival and mutation induction. The results for both varied from experiment to experiment, yielding a large SD, but the μG sample results differed significantly from the 1G sample results for each of 2 experiments, with the mean ratio of μG to 1G being 0.55 for the concentration of viable cells and 0.59 for the fraction of thymidine kinase deficient (TK⁻) mutants. Among the mutants, non-loss of zygosity events (point mutations) were less frequent (31%) after μG incubation than after 1G incubation, which might be explained by the influence of μG on cellular metabolic or physiological function. Additional experiments are needed to clarify the effect of μG interferes on DNA repair.     

Height-latitude structure of stationary planetary waves in the stratosphere and lower mesosphere

Daily UK Met Office stratospheric assimilated data for the Northern and Southern Hemispheres, accumulated for the period from 2004 to 2012 and pressure range of 1000–0.1 hPa, are used in this paper. The paper presents and thoroughly discusses spatial–temporal distributions of stationary planetary wave (SPW) amplitudes and phases, calculated on the basis of geopotential height, temperature, zonal and meridional wind data for zonal wave numbers 1 and 2 (SPW1 and SPW2). The climatological planetary wave amplitudes and phases are calculated by extracting waves from three types of data: daily, monthly mean and climatological monthly mean. It has been established that magnitude of amplitudes and height-latitude distribution of amplitudes of SPW1 and SPW2 depend on data processing method for all parameters. It has also been established that height-latitude distribution amplitudes and phases significantly differ for geopotential height, temperature, zonal and meridional wind and depend on wave number and hemisphere. However, height-latitude distributions of phases are little different from each other for the used methods of data processing.     

Geomagnetic effects on mid-latitude railways: A statistical study of anomalies in the operation of signaling and train control equipment on the East-Siberian Railway

Intense geomagnetically induced currents (GIC) can hamper rail traffic by disturbing signaling and train control systems. GIC threats have been a concern for technological systems at high-latitude locations due to geomagnetic disturbances driven by substorm expansion electrojet or convection electrojet intensifications. However, other geomagnetic storm processes such as storm sudden commencement (SSC) and geomagnetic pulsations can also cause GIC concerns for technological systems. We present in this paper the first evidence based on statistical data for links between geomagnetic disturbances and faulty operations (anomalies) in the functioning of railway automatics and telemetry. We analyze anomalies of automatic signaling and train control equipment which occurred in 2004 on the East-Siberian Railway (corrected geomagnetic latitude _m = 46–51°N and longitude λ_m = 168–187°E). Our results reveal a seasonal effect in the number of anomalies per train similar to the one observed in geomagnetic activity (Kp, Ap, Dst indices). We also found an increase by a factor of 3 in the total duration of daily anomalies during intense geomagnetic storms (local geomagnetic index specific to Siberian Observatory A_max > 30), with a significant correlation between the daily sum of durations of anomalies with geomagnetic activity. Special attention was paid to failures not related to recognized technical malfunctions. We found that the probability of these failures occurring in geomagnetically disturbed periods was 5–7 times higher than the average anomaly occurrence.