Preservation of cell structures in permafrost: a model for exobiology.

The present report is the first contribution toward a comprehensive fine-structural study of microbial cells from permafrost. Prokaryotes with a variety of cell wall types demonstrate high stability of cell structure after long-term cryopreservation in frozen soils and sediments of the Arctic. The surface capsular layers that were a salient feature of the cells both in situ and on nutrient media may be an adaptation to low temperature. To the extent that permafrost regions on Earth approximate Martian conditions, preservation of cell structure there can serve as the basis for predictions about preservation in Martian permafrost sediments.     

Long-term preservation of microbial ecosystems in permafrost.

It has been established that significant numbers (up to 10 million cells per gram of sample) of living microorganisms of various ecological and morphological groups have been preserved under permafrost conditions, at temperatures ranging from -9 to -13 degrees C and depths of up to 100 m, for thousands and sometimes millions of years. Preserved since the formation of permafrost in sand-clay sediments of the Pliocene-Quaternary period and in paleosols and peats buried among them, these cells art the only living organisms that have survived for a geologically significant period of time. The complexity of the microbial community preserved varies with the age of the permafrost. Eukaryotes are found only in Holocene sediments; while prokaryotes are found to greater ages, i.e., Pliocene and Pleistocene. The diversity of microorganisms decreases with increasing age of sediments, and as a result cocci and corynebacteria are predominant. Enzyme activity (catalase and hydrolytic enzymes) and photosynthetic pigments (chlorophyll and pheophytin have also been detected in permafrost sediments. These results permit us to outline some approaches to the search for traces of life in the permafrost of Martian sediments by borehole core sampling. It is in the deep horizons (and not on the planet surface), isolated by permafrost from the external conditions, that results similar to those obtained on Earth can be expected.     

Mercury: can any ice exist at subpolar regions?

The heat transfer in a regolith subsurface layer of thickness 20 m has been theoretically simulated for the areas near Mercury's north pole aiming at the clarification of the possible existence of subsurface ice formations of different form. The paper considers different models of the icy regolith structure and composition: pure uniform amorphous ice; a porous dispersive system with ice-filled pores and voids; permafrost. For comparison the heat transfer in dry iceless regolith has been considered as well. It has been shown that the line of maximum distribution of subsurface icy formations depends on the icy regolith model, but for any one in the “hot” regions it does not go below 70°. For the “cool” regions this line has been shown to go from 5° to 10° southward than that for the “hot” ones. The possible thickness of icy regolith near the pole has been estimated for different models assuming an interior heat flow of 15 mW m⁻². It has been shown that the maximum thickness of this layer takes place at the pole and is equal to 10 km for any model.     

A physical and chemical characterization of Martian permafrost as a possible habitat for viable microorganisms.

Data from experiments with model samples show that ion transfer coefficients in the water-rich permafrost on Mars must be three orders of magnitude less than those of terrestrial permafrost. The effects of low temperatures and of carbon dioxide have been accounted for. Exchange between cells and the environment is impeded in Martian permafrost. The microscopic distributional heterogeneity of concentration, pH, Eh, and other physicochemical parameters may be more pronounced in the permafrost of Mars. We present a classification of unfrozen water types in the permafrost that is based on the structures of unfrozen water films and on their functions with respect to cells. Any viable microorganisms on Mars probably exist with minimum metabolism in compact zones with energy carriers and high transfer coefficients. These zones may be microvolumes of unfrozen water in which cells accumulate.     

Microorganisms and enzyme activity in permafrost after removal of long-term cold stress

Associations of immobilized microbial cells and organic-mineral complexes containing active enzymes are resistant to long-term (from tens of thousands to millions of years) effects of extremely low temperatures. This association enables the cells to restore their metabolic activity during permafrost thawing, because interactions with the heterogenous medium is made possible by the availability of active immobilized enzymes. The long-term effect of the cold probably favors an adaptational change of microbial metabolism that activates enzymes and cells during thawing.     

Mini-belt as a fine spatial structure of the outer radiation belt in quiet and disturbed conditions

Electron flux data from LANL geostationary spacecrafts were statistically treated and ordered in a special magnetic coordinate system (effective L-coordinate and MLT). The data treating procedure allowed to obtain the dynamics of quasi-trapped electrons of different energies on effective L-shells ranging from 6.6 to 7.0. It was found that in quiet conditions a stable fine spatial structure of quasi-trapped electrons exists with maximum of fluxes near L = 6.78 and MLT=12. This structure may be looked at as an asymmetrical “mini-belt”. The position of the maximum depends on electron energy and changes with magnetic activity. The dynamics of this mini-belt for both quiet and disturbed periods is illustrated and discussed. During isolated magnetic storms the mini-belt maximum shifts in a regular manner outward and inward; a diffusion wave of quasi-trapped particles propagates from outside of the geostationary orbit and serves as a source of new particles for the mini-belt. The azimuthal geometry of this diffusion wave extracted from experimental data is illustrated. The possible role of the “mini-belt” is discussed in relation with well-known “anomalous” dynamics of the inner radiation belt.     

Experiment on "Discovery" STS 51-C: aggregation of red cells and thrombocytes in heart disease, hyperlipidaemia and other conditions.

The aim of this experiment was to study aggregation of red cells in the blood of patients with ischaemic heart disease, diabetes, hyperlipidaemia, and (silent) cancer, and in two normal donors. Reconstituted blood using IgG was also used. The instrument, the automated slit-capillary photo-viscometer (100 kg weight) was set on the middeck of the Space Shuttle. An analogous instrument was at the Kennedy Space Center. Blood was obtained from donors, anticoagulated, and adjusted to haematocrit of 30% using native plasma. Experiments took place at 25 degrees C, during which blood was forced to flow in the slit formed by two parallel glass plates. Macro and microphotography was carried out at specific intervals controlled by a computer. During stasis, lasting 6 minutes, aggregates (or clumps) of the red cells were formed. Results indicated that red cell aggregates do form under zero-G; that such aggregates are smaller than the ones obtained at one-G; that morphology is different, the zero-G showing rouleaux while one-G showing usual sludge-like clumps of red cells in all severe disorders. Platelets appeared to remain monodisperse under zero-G. Assuming that these data can be confirmed, one could suggest that zero-G affects cell-cell interaction, and may consequently influence the internal microstructure of the cell membrane and of the receptors, as well as their activity. Gravitational studies may thus open a new door on immunology and haematology in general.     

Energy deposition in the upper atmosphere in the EXCEDE III experiment

The EXCEDE III sounding rocket flight of April 27, 1990 used a 18 Ampere 2.5 keV electron beam to produce an artificial aurora in the region 90 to 115 km. A “daughter” sensor payload remotely monitored the low-energy X-ray spectrum while scanning photometers measured the spatial profile of prompt emissions of N₂⁺ (1N) and N₂ (2P) transitions (3914Å and 3805Å, respectively). Two Ebert-Fastie spectrometers measured the spectral region from 1800 to 8000Å. On the “mother” accelerator payload, the return current electron differential energy spectra were monitored by an electrostatic analyzer (up to 10 keV) and by a retarding potential analyzer (0 eV to 100 eV). We present an overview of the results from this experiment.     

Acceleration and transport of energeticparticles at CME-driven shocks

Historically, solar energetic particle (SEP) events are classified in two classes as “impulsive” and “gradual”. Whether there is a clear distinction between the two classes is still a matter of debate, but it is now commonly accepted that in large “gradual” SEP events, Fermi acceleration, also known as diffusive shock acceleration, is the underlying acceleration mechanism. At shock waves driven by coronal mass ejections (CMEs), particles are accelerated diffusively at the shock and often reach > MeV energies (and perhaps up to GeV energies). As a CME-driven shock propagates, expands and weakens, the accelerated particles can escape ahead of the shock into the interplanetary medium. These escaping energized particles then propagate along the interplanetary magnetic field, experiencing only weak scattering from fluctuations in the interplanetary magnetic field (IMF). In this paper, we use a Monte-Carlo approach to study the transport of energetic particles escaping from a CME-driven shock. We present particle spectra observed at 1 AU. We also discuss the particle “crossing number” at 1AU and its implication to particle anisotropy. Based on previous models of particle acceleration at CME-driven shocks, our simulation allows us to investigate various characteristics of energetic particles arriving at various distances from the sun. This provides us an excellent basis for understanding the observations of high-energy particles made at 1 AU by ACE and WIND.     

Investigation of magnetospheric processes with the use of a source of strong magnetic field in the ionosphere

More than 20 years ago V.P. Shabansky suggested that the magnetic system installed aboard the satellite, could be used as a physical instrument for studying the processes which occur in the near Earth space. The corresponding space scales of an artificial “magnetosphere”—“magnisphere”—are 10 m in the experiment with relatively small magnets in the ionosphere and 100 m in the solar wind. The corresponding similarity criteria are estimated. The possible scheme of the experiment with a superconducting magnet (magnetic moment 10⁵ A · m²) installed aboard the satellite is considered. The experimental complex includes a number of systems for measuring the fluxes of charged particles in a wide energy range, DC electric and magnetic fields, the electromagnetic fields in different frequency bands (from X-rays to radio). The scientific objectives are discussed in detail.     

Remote sensing of the ring current using energetic neutral atoms

Energetic neutral atom (ENA) images of the storm-time ring current obtained from the ISEE-1 spacecraft provide information for a “zero-order” global model of the energetic ion distribution. With the assumption of isotropic pressure and magnetostatic, non-convective pressure balance, the global system of electrical currents driven by the ion pressure can be calculated using Euler potentials for the divergenceless current density. Radial pressure gradients drive azimuthal currents, and azimuthal pressure gradients drive radial currents. The radial currents cause current lines in the inner magnetosphere to close in the ionosphere, forming a “partial” ring current. The intensities and locations of these field-aligned currents driven into and out of the ionosphere resemble those of the observed Region 2 current system, but not all observed properties of the Region 2 system are reproduced by the “zero-order” model.     

Optical observations of artificial clouds in the CRRES experiments

Some results of the optical observations of the barium clouds released from the satellite “CRRES” over the Caribbean are presented.     

Ultrastructural and growth indices of Chlorella culture in multicomponent aquatic systems under space flight conditions.

Submicroscopic organization of Chlorella cells cultivated under space flight conditions in three-component aquatic system has been studied. Comparison of the experimental cells with that of the controls revealed certain rearrangements of cell organelles particularly, a reduction in the amount of reserve polysaccharides in chloroplasts, increase of cell vacuolization and mitochondrion volume, complication configuration of plasmalemma evaginations and invaginations, and also disturbances in the process of cytokinesis. More over an increase in the number of Chlorella cells infected by bacteria was shown in the experimental variant. No considerable differences were established in the growth characteristics of the experimental and control populations. A comparative cytological analysis revealed general regularities of organelles in Chlorella cells cultivated under space flight condition in the uni- and multicomponent systems.     

The X-ray properties of accreting black holes

Accreting black holes in binary systems exhibit two properties that distinguish them from the vast majority of binary X-ray sources: (1) rapid ≤ 1 s variability and (2) bi-modal spectral behaviour. These findings are based primarily on the observed properties of Cyg X-1 where an estimate of the X-ray source mass from radial velocity studies indicates a mass of 10 M. The recently suggested black hole candidacy of LMC X-3 is based on a similar mass estimate. The X-ray properties are similar to those of Cyg X-1 in its “high state”. The unique spectral properties of these systems are used to search for other similar systems, and new “possible” candidates are suggested, that include several transient sources. A possible spectral connection between these systems and their more massive counterparts in AGN is discussed.     

On the non-occurrence of Type I X-ray bursts from the black hole candidates

It has been justifiably questioned if the black hole candidates (BHCs) have “hard surface” why Type I X-ray bursts are not seen from them [Narayan, R., Black holes in astrophysics, New J. Phys, 7, 199–218, 2005]. It is pointed out that a “physical surface” need not always be “hard” and could be “gaseous” in case the compact object is sufficiently hot [Mitra, A., The day of the reckoning: the value of the integration constant in the vacuum Schwarzschild solution, physics/0504076, p1–p6, 2005; Mitra, A., BHs or ECOs: A review of 90 years of misconceptions, in: Focus on Black Holes Research, Nova Science Pub., NY, p1–p94, 2005]. Even if a “hard surface” would be there, presence of strong intrinsic magnetic field could inhibit Type I X-ray burst from a compact object as is the case for Her X-1. Thus, non-occurrence of Type I bursts actually rules out those alternatives of BHs which are either non-magnetized or cold and, hence, is no evidence for existence of Event Horizons (EHs). On the other hand, from the first principle, we again show that the BHCs being uncharged and having finite masses cannot be BHs, because uncharged BHs have a unique mass M = 0. Thus the previous results that the so-called BHCs are actually extremely hot, ultramagnetized, Magnetospheric Eternally Collapsing Objects (ECOs) [Robertson, S., Leiter, D., Evidence for intrinsic magnetic moment in black hole candidates, Astrophys. J., 565, 447–451, (astro-ph/0102381), 2002 ; Robertson, S., Leiter, D., MECO model of galactic black hole candidates and active galactic nuclei, in: New Developments in Black Hole Research, Nova Science Pub., NY, p1–p44, astro-ph/0602453, 2005] rather than anything else get reconfirmed by non-occurrence of Type I X-ray bursts in BHCs.     

Molecular aspects of adaptation to extreme cold environments

Here are reviewed and summarized the strategies adopted by living organisms to survive low temperatures, from a molecular and membrane point of view. The presentation is aimed at a wide variety of readers.

Two prime examples of connections between biological cold adaptation and the molecular level are (1) antifreeze proteins in fish from cold sea water, (the DNA sequence of the protein gene is now known) (2) the fluidity characteristics of cell membranes in a wide variety of organisms. In model membranes of phospholipids, stabler “s-phases” have recently been found to form at low temperatures. Antarctic endolithic organisms, living just under the surface of rocks, are exposed to long periods of low temperatures, and may develop such phases in their membranes. In the saturated phosphatidyl cholines, only lipids with a restricted range of acyl chain lengths show simultaneously s-phases and a main transition : This restricted range is about the restricted range found in natural membranes. The s-phases also form in the presence of natural cryoprotectants, and may be connected with botanical vernalization.     

Images of optically thick artificial aerosol clouds in the near-earth space

The images were constructed for three aerosol cloud distribution types: uniform, one with a cavity in the center (shell) and one with a dense core (core with “wings”). Differences between images of optically thick and optically thin clouds for these three distribution types of particles and warious view angles are discussed. Calculated results are compared with experimental data from aerosol clouds observations.     

Prognoz-6 data about ultraviolet sky background in dark and Milky Way regions of the sky

Ultraviolet spectra (1100–1900 Å) of the sky background of 10 wide angle (6°×6°) regions obtained between 70 000 km and 200 000 km from the Earth with the photoelectric spectrometer “GALACTIKA” on board the satellite “PROGNOZ-6” are considered. The spectral energy distribution of the sky background, after subtraction of the stellar component, is similar for regions on both sides of the Milky Way and exhibits a strong U.V. light contribution. On the contrary, the Milky Way (l_II=190° b_II=+6°) is less rich in far U.V. light; this can be related to the predominance of an expected selective absorption near the galactic plane. The nature of U.V. excess at high galactic latitudes needs further investigations.     

Re-entry of orbital debris observed by Chung-Li VHF radar with MEDAC

MEDAC (Meteor Echo Detection and Collection) system, a product of University of Colorado, has become part of Chung-Li VHF facilities since July 12, 1989. MEDAC is installed to observe the mesospheric winds from Doppler echos returned by meteor trails in the upper atmosphere. However, the time variations in the in-phase and quadrature components of the signals can be used to derive the time history of the meteor trail formation. The meteor flight speed in the atmosphere is hence deduced. Preliminary analysis of some data taken from July 12 to July 17 of 1989 indicates that there are some “meteor” trails that could have been produced by the reentry of orbital debris into the atmosphere. The criteria of the flight speed and the ionization height are used for selecting an orbital debris trail from pools of “meteor” trails. The relative flux intensities between the reentry orbital debris flux as tentatively identified in this paper and the meteor flux is about 1 to 100.     

Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

1.

Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.