Determining the potential productivity of food crops in controlled environments.

The quest to determine the maximum potential productivity of food crops is greatly benefitted by crop growth models. Many models have been developed to analyze and predict crop growth in the field, but it is difficult to predict biological responses to stress conditions. Crop growth models for the optimal environments of a Controlled Environment Life Support System (CELSS) can be highly predictive. This paper discusses the application of a crop growth model to CELSS; the model is used to evaluate factors limiting growth. The model separately evaluates the following four physiological processes: absorption of PPF by photosynthetic tissue, carbon fixation (photosynthesis), carbon use (respiration), and carbon partitioning (harvest index). These constituent processes determine potentially achievable productivity. An analysis of each process suggests that low harvest index is the factor most limiting to yield. PPF absorption by plant canopies and respiration efficiency are also of major importance. Research concerning productivity in a CELSS should emphasize: 1) the development of gas exchange techniques to continuously monitor plant growth rates and 2) environmental techniques to reduce plant height in communities.     

An overview of Japanese CELSS research activities.

Many research activities regarding Controlled Ecological Life Support System (CELSS) have been conducted and continued all over the world since the 1960's and the concept of CELSS is now changing from Science Fiction to Scientific Reality. Development of CELSS technology is inevitable for future long duration stays of human beings in space, for lunar base construction and for manned mars flight programs. CELSS functions can be divided into two categories, Environment Control and Material Recycling. Temperature, humidity, total atmospheric pressure and partial pressure of oxygen and carbon dioxide, necessary for all living things, are to be controlled by the environment control function. This function can be performed by technologies already developed and used as the Environment Control Life Support System (ECLSS) of Space Shuttle and Space Station. As for material recycling, matured technologies have not yet been established for fully satisfying the specific metabolic requirements of each living thing including human beings. Therefore, research activities for establishing CELSS technology should be focused on material recycling technologies using biological systems such as plants and animals and physico-chemical systems, for example, a gas recycling system, a water purifying and recycling system and a waste management system. Based on these considerations, Japanese research activities have been conducted and will be continued under the tentative guideline of CELSS research activities as shown in documents /1/, /2/. The status of the over all activities are discussed in this paper.     

Closed and continuous algae cultivation system for food production and gas exchange in CELSS.

In CELSS (Controlled Ecological Life Support System), utilization of photosynthetic algae is an effective means for obtaining food and oxygen at the same time. We have chosen Spirulina, a blue-green alga, and have studied possibilities of algae utilization. We have developed an advanced algae cultivation system, which is able to produce algae continuously in a closed condition. Major features of the new system are as follows. (1) In order to maintain homogeneous culture conditions, the cultivator was designed so as to cause a swirl on medium circulation. (2) Oxygen gas separation and carbon dioxide supply are conducted by a newly designed membrane module. (3) Algae mass and medium are separated by a specially designed harvester. (4) Cultivation conditions, such as pH, temperature, algae growth rate, light intensity and quantity of generated oxygen gas are controlled by a computer system and the data are automatically recorded. This equipment is a primary model for ground experiments in order to obtain some design data for space use. A feasibility of algae cultivation in a closed condition is discussed on the basis of data obtained by use of this new system.     

Dynamic optimization of CELSS crop photosynthetic rate by computer-assisted feedback control.

A procedure for dynamic optimization of net photosynthetic rate (Pn) for crop production in Controlled Ecological Life-Support Systems (CELSS) was developed using leaf lettuce as a model crop. Canopy Pn was measured in real time and fed back for environmental control. Setpoints of photosynthetic photon flux (PPF) and CO2 concentration for each hour of the crop-growth cycle were decided by computer to reach a targeted Pn each day. Decision making was based on empirical mathematical models combined with rule sets developed from recent experimental data. Comparisons showed that dynamic control resulted in better yield per unit energy input to the growth system than did static control. With comparable productivity parameters and potential for significant energy savings, dynamic control strategies will contribute greatly to the sustainability of space-deployed CELSS.     

CELSS transportation analysis

Regenerative life support systems based on the use of biological material have been considered for inclusion in manned spacecraft since the early days of the United States space program. These biological life support systems are currently being developed by NASA in the Controlled Ecological Life Support System (CELSS) program. Because of the progress being achieved in the CELSS program, it is time to determine which space missions may profit from use of the developing technology. This paper presents the results of a study that was conducted to estimate where potential transportation cost savings could be anticipated by using CELSS technology for selected future manned space missions.

Six representative missions were selected for study from those included in NASA planning studies. The selected missions ranged from a low Earth orbit mission to those associated with asteroids and a Mars sortie. The crew sizes considered varied from four persons to five thousand. Other study parameters included mission duration and life support closure percentages, with the latter ranging from complete resupply of consumable life support materials to 97% closure of the life support system. The paper presents the analytical study approach and describes the missions and systems considered, together with the benefits derived from CELSS when applicable.     

Controlled Ecological Life Support Systems (CELSS) flight experimentation.

The NASA CELSS program has the goal of developing life support systems for humans in space based on the use of higher plants. The program has supported research at universities with a primary focus of increasing the productivity of candidate crop plants. To understand the effects of the space environment on plant productivity, the CELSS Test Facility (CTF) has been been conceived as an instrument that will permit the evaluation of plant productivity on Space Station Freedom. The CTF will maintain specific environmental conditions and collect data on gas exchange rates and biomass accumulation over the growth period of several crop plants grown sequentially from seed to harvest. The science requirements of the CTF will be described, as will current design concepts and specific technology requirements for operation in micro-gravity.     

Atmosphere behavior in gas-closed mouse-algal systems: An experimental and modelling study

Concepts of biologically-based regenerative life support systems anticipate the use of photosynthetic organisms for air revitalization. However, mismatches in the rates of production and uptake of oxygen or carbon dioxide between the crew and the plants will lead to an accumulation or depletion of these gases beyond tolerable limits. One method for correcting these atmospheric changes is to use physicochemical devices. This would conflict with the constraint of minimal size and weight imposed upon the successful development of a competitive bioregenerative system. An alternate control strategy is based upon reducing the gas exchange mismatch by manipulation of those environmental parameters known to affect plant or algae gas exchange ratios. We have initiated a research program using a dual approach of mathematical modelling and laboratory experimentation aimed at examining the gas exchange characteristics of artificial animal/plant systems closed to the ambient atmosphere. Our goal is to develop control techniques and management strategies for maintaining the atmospheric levels of carbon dioxide and oxygen at physiological levels. A mathematical model simulating the atmospheric behavior in these systems has been developed and an experimental gas-closed system has been constructed. These will be described and preliminary results will be presented.     

UML在软件可靠性测试数据自动生成中的运用

为了自动生成实时嵌入式软件的可靠性测试数据,利用UML(统一建模语言)提供的建模机 制,从软件可靠性测试的角度建立use-case剖面、接口模型和测试数据实现模型,然后根 据 这些模型的描述进行随机抽样,自动生成可靠性测试数据.利用该方法能够开发出相应的测 试数据自动生成工具,在软件可靠性测试领域有着较大的实用价值.     

Development of autonomous control in a closed microbial bioreactor.

Space-based life support systems which include ecological components will rely on sophisticated hardware and software to monitor and control key system parameters. Autonomous closed artificial ecosystems are useful for research in numerous fields. We are developing a bioreactor designed to study both microbe-environment interactions and autonomous control systems. Currently we are investigating N-cycling and N-mass balance in closed microbial systems. The design features of the system involve real-time monitoring of physical parameters (e.g. temperature, light), growth solution composition (e.g. pH, NOx, CO2), cell density and the status of important hardware components. Control of key system parameters is achieved by incorporation of artificial intelligence software tools that permit autonomous decision-making by the instrument. These developments provide a valuable research tool for terrestrial microbial ecology, as well as a testbed for implementation of artificial intelligence concepts. Autonomous instrumentation will be necessary for robust operation of space-based life support systems, and for use on robotic spacecraft. Sample data acquired from the system, important features of software components, and potential applications for terrestrial and space research will be presented.     

Synergies between plant research conducted for terrestrial and for space purposes.

During the past 10 years, the main part of CELSS studies has concerned the exploration of limits of plant productivity. Very high yields were obtained in continuous and high lighting, without reaching any limit. Concepts of mineral nutrition were renewed. CELSS activities now induce a development in the techniques of image processing applied to plants in order to follow the growth, to detect stresses or diseases or to pilot harvesting robots. Notable efforts concern the development of sensors, the study of trace contaminants and the micro-organisms monitoring. In parallel, several instruments for plant culture in closed Systems were developed. The advantages of closure are emphasised in comparison with open flow systems. The concept of Artificial Ecosystems developed for space research is more and more taken into account by the scientific community. It is considered as a new tool to study basic and applied problems related to ecology and not especially concerned with space research.     

Effects of bioreactor retention time on aerobic microbial decomposition of CELSS crop residues.

The focus of resource recovery research at the KSC-CELSS Breadboard Project has been the evaluation of microbiologically mediated biodegradation of crop residues by manipulation of bioreactor process and environmental variables. We will present results from over 3 years of studies that used laboratory- and breadboard-scale (8 and 120 L working volumes, respectively) aerobic, fed-batch, continuous stirred tank reactors (CSTR) for recovery of carbon and minerals from breadboard grown wheat and white potato residues. The paper will focus on the effects of a key process variable--bioreactor retention time--on response variables indicative of bioreactor performance. The goal is to determine the shortest retention time that is feasible for processing CELSS crop residues, thereby reducing bioreactor volume and weight requirements. Pushing the lower limits of bioreactor retention times will provide useful data for engineers who need to compare biological and physicochemical components. Bioreactor retention times were manipulated to range between 0.25 and 48 days. Results indicate that increases in retention time lead to a 4-fold increase in crop residue biodegradation, as measured by both dry weight losses and CO2 production. A similar overall trend was also observed for crop residue fiber (cellulose and hemicellulose), with a noticeable jump in cellulose degradation between the 5.3 day and 10.7 day retention times. Water-soluble organic compounds (measured as soluble TOC) were appreciably reduced by more than 4-fold at all retention times tested. Results from a study of even shorter retention times (down to 0.25 days), in progress, will also be presented.     

Achieving and documenting closure in plant growth facilities.

As NASA proceeds with its effort to develop a Controlled Ecological Life Support System (CELSS) that will provide life support to crews during long duration space missions, it must address the question of facility and system closure. Here we discuss the concept of closure as it pertains to CELSS and describe engineering specifications, construction problems and monitoring procedures used in the development and operation of a closed plant growth facility for the CELSS program. A plant growth facility is one of several modules required for a CELSS. A prototype of this module at Kennedy Space Center is the large (7m tall x 3.5m diameter) Biomass Production Chamber (BPC), the central facility of the CELSS Breadboard Project. The BPC is atmospherically sealed to a leak rate of approximately 5% of its total volume per 24 hours. This paper will discuss the requirements for atmospheric closure in this facility, present CO2 and trace gas data from initial tests of the BPC with and without plants, and describe how the chamber was sealed atmospherically. Implications that research conducted in this type of facility will have for the CELSS program are discussed.     

Novel sensor technology for monitoring and control of critical plant nutrient parameters.

A novel dielectric sensor technology has been developed for monitoring and control of plant nutrient delivery systems as part of NASA's Controlled Ecological Life Support System (CELSS) program. A unique measurement phenomenon was discovered in which the electrostatic field is shunted to a third terminal of the sensor, resulting in a much greater sensitivity to changes in the complex dielectric properties of the nutrient solution. Based on this phenomenon, a small, flexible, thin-film shunting dielectric sensor (SDS) was designed to provide low-frequency, non-invasive measurement of both the thickness and nutrient concentration of the layer of solution on a plant growth surface. Test results indicate a sensitivity of +/- 0.05mm in layer thickness while characterization of the ability to measure nutrient concentration continues. The development plan for this sensor is presented and other applications are discussed.     

Preliminary experimental results of gas recycling subsystems except carbon dioxide concentration.

Oxygen concentration and separation is an essential factor for air recycling in a CELSS. Furthermore, if the value of the plant assimilatory quotient is not coincident with that of the animal respiratory quotient, the recovery of O2 from the concentrated CO2 through chemical methods will become necessary to balance the gas contents in a CELSS. Therefore, oxygen concentration and separation equipment using Salcomine and O2 recovery equipment, such as Sabatier and Bosch reactors, were experimentally developed and tested.     

DFD风洞测控系统设计

DFD风洞测控和数据采集处理系统以工业控制计算机为核心,通过PCL-818A/D数据采集卡完成试验数据采集、反馈信息入口和控制指令出口,实时进行实验条件监测。系统软件采用VB6.0编制,实现了风洞风速闭环控制、模型姿态处理调整、流场品质测量及全机模型低速风洞实验等。该系统造价低,操作界面友好,使用维护方便,具有很好的实用性。     

CELSS system control: issues, methods, and directions.

In the general control perspective, the CELSS concept implies a very complex system and presents challenges at every level. These challenges are generated by: (1) the prospect that the system will be inherently unstable, (2) the prospective difficulty of establishing an adequate mathematical model of the system for the purpose of control law synthesis (dimensionality is high, and the dynamics and interactive processes of some of the subsystems are not understood well), (3) assuring control law robustness (assuring that the resulting control law(s) will be effective over the domain of the specified uncertainties), (4) hardware realization of the control law, (5) hardware system robustness ("fault tolerance") and (6) achieving the logistics of the automation (or "management") aspects of the problem. A suggested organization of the problem, a sketch of the issues related to perceived difficulties, a commentary/evaluation of the issues, a review of methods available to address the issues, and a suggested strategy to address the broad CELSS systems control problem are presented.     

Earth life support for aquatic organisms, system and technical aspects.

The importance of the research on Bioregenerative Life Support has increased dramatically in the last decade not only with regard to possible space flight application but also as a way to obtain a better understanding of our Earth's ecology. A major goal was to reach long-term stability of artificial model systems. Preliminary data are presented on the development of an improved aquatic system, currently dedicated for ground-based research. Closed aquatic ecosystems require reliability of the key parameters of pH, O2 and CO2 concentration and stability of sensors for monitoring. Besides the integration of an artificial lung (holofiber system and air pump with valves, allowing controlled oxygen uptake of air), in parallel to the oxygen producing water plants. Our new approach is to implement opto-chemical sensors, for such environmental monitoring. One major advantage of the new sensor technique is their better long-term reliability as compared to the electrochemical sensors. Our experiment with the new sensor technique has demonstrated satisfactory performance in closed aquatic ecosystems.     

The Breadboard Project: a functioning CELSS plant growth system.

The primary objective of the Breadboard project for the next 3-4 years is to develop, integrate and operate a Controlled Ecological Life Support System (CELSS) at a one person scale. The focus of this project over the past two years has been the development of the plant growth facility, the first module of the CELSS. The other major modules, food preparation, biomass processing, and resource recovery, have been researched at the laboratory scale during the past two years and facilities are currently under construction to scale-up these modules to an operational state. This paper will outline the design requirements for the Biomass Production Chamber (BPC), the plant growth facility for the project, and the control and monitoring subsystems which operate the chamber and will present results from both engineering and biological tests of the facility. Three production evaluations of wheat, conducted in the BPC during the past year, will be described and the data generated from these tests discussed. Future plans for the BPC will be presented along with future goals for the project as the other modules become active.     

Utilization of sweet potatoes in controlled ecological life support systems (CELSS).

A number of studies have selected the sweet potato as a potentially important crop for CELSS. Most hydroponic studies of sweet potatoes have been short term (<80 days). Full term (90 to 150 days) studies of sweet potatoes in hydroponic systems were needed to understand the physiology of storage root enlargement and to evaluate sweet potato production potential for CELSS. Early and late maturing sweet potato varieties were crown in hydroponic systems of different types--static with periodic replacement, flowing with and without recirculation, aggregate, and non-aggregate. In a flowing system with recirculation designed at Tuskegee University using the nutrient film technique (NFT), storage root yields as high as 1790 g were produced with an edible growth rate of up to 66 g m-2 d-1 and a harvest index as high as 89% under greenhouse conditions. Preliminary experiments indicated high yields can be obtained in controlled environmental chambers. Significant cultivar differences were found in all systems studied. Nutritive composition of storage roots and foliage were similar to field-grown plants. The results indicate great potential for sweet potato in CELSS.     

Operation of an experimental algal gas exchanger for use in a CELSS.

Concepts of a CELSS anticipate the use of photosynthetic organisms (higher plants and algae) for air revitalization. The rates of production and uptake of carbon dioxide and oxygen between the crew and the photosynthetic organisms are mismatched. An algal [correction of aglal] system used for gas exchange only will have the difficulty of an accumulation or depletion of these gases beyond physiologically tolerable limits (in a materially closed system the mismatch between assimilatory quotient (AQ) and respiratory quotient (RQ) will be balanced by the operation of the waste processor). We report the results of a study designed to test the feasibility of using environmental manipulations to maintain physiologically appropriate atmospheres for algae (Chlorella pyrenoidosa) and mice (Mus musculus strain DW/J) in a gas-closed system. Specifically, we consider the atmosphere behavior of this system with Chlorella grown on nitrate or urea and at different light intensities and optical densities. Manipulation of both the photosynthetic rate and AQ of the alga has been found to reduce the mismatch of gas requirements and allow operation of the system in a gas-stable manner. Operation of such a system in a CELSS may be useful for reduction of buffer sizes, as a backup system for higher plant air revitalization and to supply extra oxygen to the waste processor or during crew changes. In addition, mass balance for components of the system (mouse, algae and a waste processor) are presented.