Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness

We have explored the role of Ca²⁺ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca²⁺]_i), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca²⁺]_i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca²⁺]_i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca²⁺ influx through stretch-activated channels, then results in microfilament disruption.     

Global comparison of the model results of GSM TIP with IRI for summer conditions

This paper presents the results of the numerical calculations thermosphere/ionosphere parameters which were executed with using of the Global Self-consistent Model of the Thermosphere, Ionosphere and Protonosphere (GSM TIP)and comparison of these results with empirically-based model IRI-2001. Model GSM TIP was developed in West Department of IZMIRAN and solves self-consistently the time-dependent, 3-D coupled equations of the momentum, energy and continuity for neutral particles (O₂, N₂, O), ions (O⁺, H⁺), molecular ions (M⁺) and electrons and largescale eletric field of the dynamo and magnetospheric origin in the range of height from 80 km to 15 Earth’s radii. The empirically derived IRI model describes the E and F regions of the ionosphere in terms of location, time, solar activity and season. Its output provides a global specification not only of N_e but also on the ion and electron temperatures and the ion composition. These two models represent a unique set of capabilities that reflect major differences in along with a substantial approaches of the first-principles model and global database model for the mapping ionosphere parameters. We focus on global distribution of the N_e, T_i, T_e and TEC for the one moment UT and fixed altitudes: 110 km, h_mF2, 300 km and 1000 km. The calculations were executed with using of GSM TIP and IRI models for August 1999, moderate solar activity and quiet geomagnetic conditions. Results present as the global differences between the IRI and GSM TIP models predictions. The discrepancies between model results are discussed.     

Imaging Global Electron Content backwards in time more than 160 years ago

The Global Electron Content, GEC, represents the total number of electrons in the spherical layer over the Earth restricted by orbit of Global Positioning Satellite system (20,200 km). GEC is produced from Global Ionospheric Map of Total Electron Content, GIM-TEC, transformed to the electron density varying with height using the International Reference Ionosphere and Plasmasphere model, IRI-Plas. The climatologic GEC model is developed from GIM-TEC maps for a period 1999–2012 including the solar activity, annual and semiannual cycles as the most important factors affecting daily GEC variation. The proxy R_zp of the international sunspot numbers, R_i, is used as a measure of solar activity composed of 3 day smoothed R_i, 7 day and 81 day backwards mean of R_i scaled to the range of 1–40 proxy units, p.u. The root mean square error of the GEC climatologic model is found to vary from 8% to 13% of GEC. Taking advantage of a long history of sunspot numbers, the climatologic GEC model is applied for GEC reconstruction backwards in time for more than 160 years ago since 1850. The extended set of GEC values provides the numerical representation of the ionosphere and plasmasphere electron content coherent with variations of solar activity as a potential proxy index driving the ionosphere models.     

Short wavelength ion-cyclotron waves upstream of the earth's bow shock

Ion beams reflected from the bow shock are shown to excite ioncyclotron instability in the solar wind. For the parameters relevant to the solarwind upstream of the terrestrial bow shock the growth

is found to be a significant fraction of the ion cyclotron frequency μ_i for K

_M ≳ 2. Here K is the wave vector and

_M is the proton gyro radius. The corresponding wave frequency ω_r is found to be from ∼ 0.5 to several times the ion gyrofrequency μ_i. Since the group velocities of these waves are small they can efficiently interact with the plasma in and near the generating region.     

Ion precipitation into the ionosphere during geomagnetic storms

This review presents numerous recent examples of interesting variations in the composition and intensity of the hot ion flux (10 eV - 15 keV/e) provided by the AUREOL-3 satellite as a function of latitude and local time during periods of magnetic activity. In particular, these results reveal that although H⁺ is the most abundant ion during magnetically quiet periods, the ion composition of hot plasma at ionospheric altitudes is quite variable, and depends strongly on magnetic activity; results obtained during main and recovery phases of several magnetic storms demonstrate clearly (below 15 keV/Q) the great importance of the low altitude ionospheric source (H⁺, O⁺, and to a lesser degree He⁺) particularly at low latitudes (L ～ 3 - 4) where the flux of O⁺ ions becomes very large and even dominates. The results of the AUREOL-3 ion spectrometers establish the fact that upflowing suprathermal ionospheric ions (E_i < 100 eV/e) appear over large regions of the auroral ionosphere, the polar caps, and the polar cusp, as well as in or at the boundary of the plasmasphere during magnetospheric substorms or magnetic storms, and may consequently contribute significantly to the plasma sheet and to the inner storm time ring current. Most of the properties of the storm time ring current found by the GEOS, SCATHA, and ISEE satellites apply to lower altitudes, although the role of the ionospheric and/or plasmaspheric source appears accentuated.     

The investigation of powerful solar flares characteristics by analysis of excited states of C and various neutrons capture lines

Accelerated energetic particles in solar flares produced nuclear γ-lines in interactions with ambient solar atmosphere. Analysis of intensity of ratios between various γ-lines allows us to make estimations of abundance of elements, parameters of surrounding media and other solar characteristics. In this article we discuss the flux ratio between two lines from excited states of ¹²C (f_15.11/f_4.44) and our results of preliminary calculation of intensity ratio between two neutron capture lines at ³He and ¹H (f_20.58/f_2.223). In particular we consider the opportunity to obtain n(³He)/n(¹H) ratio during solar flares and using high-energy gamma-emission studying, based on the satellite data. Possible interpretation of spectral features observed during the January 20, 2005 solar flare is discussed. Preliminary analysis of energy spectrum in the band of 2–21 MeV gives n(³He)/n(¹H) ∼ 8 × 10⁻⁴ for January 20, 2005 solar flare.     

High-density and high-temperature molecular gas around the AGN in M51

We investigated the physical properties of molecular gas in the nuclear region of M51 (Seyfert 2). We obtained an aperture synthesis ¹³CO(J = 1 − 0) image using the Nobeyama Millimeter Array (NMA), and compared it with NMA ¹²CO(J = 1 − 0) and HCN(J = 1 − 0) maps at similar spatial resolutions. Within a radius of 180 pc from the center, the ¹³CO(1 − 0) integrated intensity was found to be 3 times weaker than that of HCN(1 − 0). Large-Velocity-Gradient (LVG) calculations suggest that the observed high HCN(1 − 0)/¹³CO(1 − 0) intensity ratio would arise from dense (n_H2 ∼ 10⁵ cm⁻³) and hot (T_kin ≳ 300 K) molecular clouds in the nuclear molecular disk. We also observed in the ¹²CO(1 − 0), (3 − 2), ¹³CO(1 − 0), and (3 − 2) lines using the Nobeyama 45m and JCMT 15m telescopes. We detected weak ¹³CO lines as well as strong ¹²CO lines. The LVG calculations assuming a two-component model suggest that there is a large amount of low-density (n_H2 ∼ 3 − 6 × 10² cm⁻³), low-temperature (T_kin ∼ 20 – 50 K) gas, and a small amount of high-density (n_H2 ≳ 10⁴ cm⁻³), high-temperature (T_kin ≳ 500 K) gas. The existence of the high-density and high-temperature component, although having a quite small beam filling factor, supports the aperture synthesis observation results mentioned above. Since this dense, hot gas is located in the nuclear molecular disk around the Active Galactic Nucleus (AGN), it may be heated by the strong X-ray radiation and/or by the shock induced by the radio jet.     

Low energy observations of Cygnus X-2 by Ariel VI

Ariel VI observations of Cygnus X-2 have revealed a rather flat spectrum between 0.1 and 1.5 keV with variable emission at low energy. Of the two conflicting interpretations of this object in terms of i) a distant high-luminosity (L_x 10³⁸ ergs s⁻¹) binary and ii) a nearby low-luminosity (L_x 10³⁵ ergs s⁻¹) degenerate dwarf system, our measurements support the latter.     

Hall MHD reconnection with an initial guide field By0

An uniform out-of-plane magnetic field component B_y0 is added to the equilibrium Harris sheet with plasma β = 0.5 and L_c = 0.5d_i (where L_c is the half-width of the equilibrium current layer and d_i is the ion inertial length). Driven by the continuous boundary inflows, the magnetic reconnections with the guide field B_y0/B₀ ranging from 0 to 4.0 are investigated using a 2.5D Hall magnetohydro-dynamic (MHD) code developed from a multi-step implicit scheme. The features of the reconnection field are substantially altered in the presence of the guide field. The openness of the magnetic separatrix angle is slightly reduced and the anti-symmetric quadrupolar structure of B_y field and the symmetric distribution of plasma pressure P are replaced by an asymmetric B_y four-wing structure and an asymmetric P plot as a non-zero B_y0 is added. The decoupling of electrons and ions also occurs near the X line in the case with a finite B_y0, but the effect of initial B_y0 on the electron flow is greater than that on the ion flow. The reconnection rates at the X-line drops from 0.151 to 0.06, namely, ∂A/∂t is reduced by a factor of 2.5 as B_y0/B₀ increases from 0 to 4.0. The reduction of reconnection rate might be related to the reducing openness of reconnection layer with the increasing B_y0.     

Mass spectrometric measurements of stratospheric ions

Recent in situ measurements with balloon borne quadrupole mass spectrometers, between 20 and 45 km altitude, are reviewed and discussed.The major stratospheric positive ions observed are proton hydrates [H⁺(H₂O)_n] and non proton hydrates of the form H⁺X_m(H₂O)₂. The data analysis allows a derivation of the vertical mixing ratio profile of X (most probably CH₃CN), which is compared with recent model calculations. From negative ion composition data, showing the presence of NO₃^? and HSO₄^? cluster ions, the density of sulfuric acid in the stratosphere is deduced. The implications of these findings on our understanding of the sulfur chemistry is briefly treated.Finally some other aspects such as contamination, cluster break up and the use of stratospheric ion mass spectra for determination of thermochemical data and other minor constituents are discussed.     

Energy conversion of the flare due to direct electric fields from the sheared reconnection

In this paper we present a new mechanism of the main energy conversion of the solar flare. Since a flare inducing prominence (flux tube) rises V_z ? 300 km s⁻¹, the plasmas below it cannot continuously eject with Alfvén speeds of V_A = 3000 km s⁻¹ but probably with V_z ≈ ±100 km s⁻¹. Plasma up and downflows with V_A will within a short duration be blocked between the chromosphere where reconnected flux tubes are piling up, and the slowly rising flux rope. Hence the Petschek slow shock mechanism is difficult to be realized as a major energy converting mechanism.     

Planetary magnetotails: Model based on ISEE-3 and voyager-2 evidence

It is shown that one can understand the basic properties of the quiet distant Earth's / and Jupiter's magnetotails as seen in the ISEE-3 / and Voyager-2 data (at distances ∼ 200 R_E / and ∼ 6 000 R_J correspondingly behind the planets) within a simple hydromagnetic model with a partially “open” boundary. The degree of openness required to explain the lobe densities (≳ 10⁻¹ / ≳ 10⁻² cm⁻³) is ∼ 10⁻¹ in both cases. This gives the average lobe magnetic field intensities: 8–9 / 0–0.5 nT. The tail flaring rate decreases at ∼ 100 R_E / ∼ 1 000 R_J, and for larger distances non-circular tails are obtained with the average diameters ∼ 60 R_E / ∼ 600 R_J, in agreement with the data. The degree of flattening (east-west to north-south ratio) is 1.2–2.3 / 4–10 depending on the pressure anisotropy in the solar wind. The expected lengths of the magnetic tails of Mercury, Saturh, Uranus are: 10–50 R_M, (1–7)×10³ R_S, (1–6) × 10³ R_U, in corresponding planetary radii.     

Nightglow studies with the world's largest optical telescope

By making use of 0.02-nm-resolution sky spectra from the HIRES echelle spectrograph at the W.M. Keck(I) observatory on Mauna Kea, HI, obtained during normal astronomical observations, we have shown that 650–870 nm emission from the vibrationally-excited levels of the O₂(b¹Σ⁺_g) state is a significant component of the terrestrial nightglow, with a total average intensity from levels v = 1–15 of approximately 150 R. The b¹Σ⁺_g state vibrational distribution is bimodal, with peaks at v = 3, 4 and v = 12, and a deep minimum at v = 8 containing only ∼5% of the v = 3 population. The b-X 0-0 band (the Franhofer A-band) is discernible via isotopic emission, with an intensity comparable to that of the strongest of the new b-X bands. There are indications that the vibrationally-excited b¹Σ⁺_g state emissions correlate temporally with the OH Meinel band emission, and satellite measurements appear to show that the relatively strong emission from the O₂(b-X) 4-3 band originates at an alitutde near 87 km, i.e. in the OH region.In an 8-minute observation on 20 Nov 1999, the Keck telescope was pointed at Venus, and the first high-resolution nightglow spectrum was recorded in the visible spectral region. The oxygen green line was revealed, with an intensity near 150 R, approximately the same as in the terrestrial atmosphere. The only previous observation, from the Venera 9/10 orbiters, did not detect this emission.     

Ionosphere-plasmasphere electron fluxes at middle latitudes obtained from whistlers

985 whistlers observed between 1970 and 1975 in Hungary have been processed for equatorial plasmaspheric electron density and tube electron content above 1000 km (N_T). The hourly median value of N_T exhibits a diurnal variation with an amplitude of 1×10¹³ electrons/cm²-tube. 75 per cent of the electron flux values obtained from the time variation of N_T are lower than 6×10⁸ el cm^?2s^?1, while in some cases the fluxes reach a value as high as 3×10⁹ el cm^?2s^?1. Between 17 and 04 LT the dominant flux direction is toward the ionosphere. The data also indicate that the day to day filling of the plasmasphere after magnetic disturbances continues through several days without exhibiting saturation, with higher filling rates for lower values of average K_p.     

Model calculations of the dayside ionosphere of Venus

Model calculations of the dayside ionosphere of Venus are presented. The coupled continuity and momentum equations were solved for O₂⁺, O⁺, CO₂⁺, C⁺, N⁺, He⁺, and H⁺ density distributions, which are compared with measurements from the Pioneer Venus ion mass spectrometer. The agreement between the model results and the measurements is good for some species, such as O⁺, and rather poor for others, such as N⁺, indicating that our understanding of the dayside ion composition of Venus is incomplete. The coupled heat conduction equations for ions and electrons were solved and the calculated temperatures compared with Pioneer Venus measurements. It is shown that fluctuations in the magnetic field have a significant effect on the energy balance of the ionosphere.     

Positive ion composition and electron density in a combined auroral and NLC event

The positive ion composition and electron density were measured in the lower ionosphere above Kiruna in salvo A of CAMP (Cold Arctic Mesopause Project). The CAMP/P (S37/P) payload carrying a magnetic ion spectrometer, positive ion and electron probes, and propagation experiments was launched on 3 August 1982 2332 UT during extended Noctilucent Clouds (NLC) and auroral activities over Kiruna. The measured electron density was 5×10³cm^?3 at 80 km and 2.5×10⁵cm^?3 at 90 km. The increase of ion and electron densities in the D- and E-region during twilight was caused by precipitating auroral particles. The height distribution of the positive ions measured by the mass spectrometer in the mass range 19–280 amu is different from a winter flight with similar auroral conditions. Below 85.5 km proton hydrates H⁺(H₂O)₃ ? H⁺(H₂O)₈ were the dominant ions. The heaviest proton hydrates H⁺(H₂O)₇ and H⁺(H₂O)₈ were most abundant at 82–85.5 km, the altitude of visible NLC. Above 85.5 km O₂⁺ and NO⁺ became dominant. A small metal ion layer was observed between 90.5–93 km with a maximum ion density of 10% of the total positive ion density at 91 km altitude. The metal ion density disappeared within about a km below 90.5 km.     

The neutral particle impact de-excitation cross-section of the fine structure level3P1 of atomic oxygen in the thermosphere

A new upper limit value (9.9 × 10^?21cm²) of the mean equivalent de-excitation cross-section of O(³P₁) by collision with neutral particles in the thermosphere is obtained by comparing the³P₁ state de-excitation rate due to neutral practice impact with that due to electron impact. The existing data of the cross-section obtained from the aeronomic study are compared with the theoretical data, and it indicated that the former is much less than the latter.     

Quantitative determination of the outgassing water vapor concentrations surrounding space vehicles from ion mass spectrometer measurements

Outgassing from materials as well as deliberate gaseous and liquid releases create contaminant clouds around spacecraft that can degrade both instrumentation and measurements. This paper describes a new method for estimating outgassing water vapor concentrations around space vehicles. Water vapor ions measured in the course of a rocket experiment performed at Eglin AFB, Florida, on December 12, 1980 at 2311 UT are utilized to demonstrate the technique. The H₂O concentration near the payload's surface is calculated using the rate coefficient for the fast charge transfer process, O⁺ + H₂O + H₂O⁺ + O, the source of the observed water vapor ions. It is found that the measured H₂O⁺ ions were produced within 3–4 cm of the sampling plate's surface and that the average H₂O pressure over this distance was relatively constant on ascent at 8 × 10^?6 torr, within a factor two, implying a steady outgassing rate.     

Advanced architectures for real-time Delay-Doppler Map GNSS-reflectometers: The GPS reflectometer instrument for PAU (griPAU)

In recent years Global Navigation Satellite System’s signals Reflectometry (GNSS-R) has stood as a potential powerful remote sensing technique to derive scientifically relevant geophysical parameters such as ocean altimetry, sea state or soil moisture. This has brought out the need of designing and implementing appropriate receivers in order to track and process this kind of signals in real-time to avoid the storage of huge volumes of raw data. This paper presents the architecture and performance of the Global Positioning System (GPS) Reflectometer Instrument for PAU (griPAU), a real-time high resolution Delay-Doppler Map reflectometer, operating at the GPS L1 frequency with the C/A codes. The griPAU instrument computes 24 × 32 complex points DDMs with configurable resolution (Δf_Dmin = 20 Hz, Δτ_min = 0.05 chips) and selectable coherent (minimum = 1 ms, maximum = 100 ms for correlation loss Δρ < 90%) and incoherent integration times (minimum of one coherent integration period and maximum not limited but typically <1 s). A high sensitivity (DDM peak relative error = 0.9% and DDM volume relative error = 0.03% @ T_i = 1 s) and stability (Δρ/Δt = −1 s⁻¹) have been achieved by means of advanced digital design techniques.