SPR生物传感器在生命科学及在航天医学中的应用

表面等离子体共振生物传感器在分析生物分子相互作用方面，具有灵敏度高、实时在线、简单快捷和抗干扰能力强等优点，被广泛应用于临床检验、药物筛选等研究领域；因其能满足空间特殊环境下的使用要求，在航天医学领域具有广阔的应用前景．本文综述了表面等离子体共振生物传感器的原理、特点、在生命科学研究中的应用及在航天医学中的应用前景．     

朗缪尔探针在轨快速除污染技术研究

讨论了一种朗缪尔球形探针在轨快速去除表面污染的技术方法. 朗缪尔探针是空间等离子体原位探测的一项重要手段, 广泛应用于各种航天器. 探针传感器表面被污染时, 会造成探针I-V特性曲线失真, 从而导致探测获得的等离子体参数产生偏差. 通过试验验证了采用探针传感器加载高压的技术, 其可快速有效清除传感器表面污染, 确保朗缪尔探针在轨探测获得实时、准确的空间等离子体信息.      

LAMP耦合荧光侧向流层析试纸条的空间微生物快速检测技术

微生物种类及其含量监测是空间站内微生物控制的重要环节。但是空间环境的微重力条件及对资源的条件限制导致地面常规检测实验难以开展，因此在轨微生物检测主要依靠培养法。基于侧流层析试纸条的生物分子识别检测方法具有不受微重力环境影响的优点，耦合荧光检测方法可以达到较高的检测灵敏度，是在轨微生物检测的潜在方法之一。针对空间环境中对航天员生活环境及仪器仪表设备具有潜在危害的微生物，研究了一种基于环介导等温扩增(LAMP)耦合荧光侧流层析试纸条的微生物核酸鉴别技术。研究结果表明，该技术可实现对大肠杆菌、金黄色葡萄球菌等空间站常见有害微生物的高灵敏、高特异性、快速检测，检测时间小于60 min，灵敏度达到100 copy·μL^–1。     

多离子空间等离子体中快磁声波与粒子的回旋共振特性

快磁声波是空间等离子体中一种接近垂直传播的右旋极化电磁波,能够在等离子体层内外传播.快磁声波与带电粒子的回旋共振相互作用能够导致高能电子随机加速和投掷角扩散、能量质子投掷角扩散等,从而影响辐射带高能带电粒子的动态过程.分别基于完整的色散关系和高密度近似的色散关系,在不同空间等离子体条件下研究多离子空间等离子体中不同传播角的快磁声波色散曲线,并计算了快磁声波与H+,He+和O+离子的最小共振能量.结果表明,当传播角较小时,采用高密度近似与采用完整色散关系计算的离子最小共振能量没有太大差别.在中低密度中强磁场空间等离子体中,传播角≥ 88°时高密度近似色散关系会带来很大的误差,因此应利用完整色散关系计算最小共振能量.      

共轴共聚焦干涉式表面等离子体显微成像技术

表面等离子体(SPs)显微成像技术能够在纳米尺度上对材料折射率的局部变化以及材料的表面形貌进行检测,这一特性使其在生物医疗及半导体材料等领域有很多的应用。提出一种新型共轴共聚焦干涉式表面等离子体显微成像技术,该技术可以定量地对折射率变化进行检测,而且具有实现简单、成本低、对环境条件要求低、信噪比高等优点。采用压电陶瓷微纳米移动平台在显微物镜的焦面附近对样品进行扫描,SPs信号与参考光的相对相位会改变从而产生一个周期性的振荡信号即V(z)曲线。同时该技术能够通过控制样品的离焦距离来实现图像对比度的可控,而且这一举措不会显著地降低图像的分辨率及对比度。也分别从理论仿真和实验结果上证明了该技术的可行性。     

太阳帆板驱动机构的表面充放电效应研究

空间等离子体环境效应导致的卫星表面充放电是造成卫星在轨工作异常及故障的重要原因之一. 太阳帆板驱动机构（Solar Array Drive Assembly,SADA）是长寿命、大功率卫星电传输环节的关键部件,易成为充放电效应的对象,可使卫星丧失能源,导致整星失效. 为验证空间等离子体环境导致的表面充放电对SADA特别是其功率传输可靠性和安全性的影响,利用等离子体环境模拟试验装置,模拟地球同步轨道（Geostationary Orbit,GEO）等离子体环境,针对SADA进行试验研究. 结果表明,使用两种不同绝缘材料的SADA在空间等离子体模拟环境下表现没有明显区别,表面充放电未对设计合理的SADA正常工作造成明显影响. 研究结果对未来GEO轨道SADA等空间机构的可靠性和安全性设计具有一定指导意义.      

等离子体处理对非晶态合金镀层表面改性研究

通过微波等离子体处理方法对Ni-W、Ni-W-B非晶态合金镀层表面进行改性的研究,测量了表面改性对非晶态镀层耐蚀性、表面成分及结构的影响.结果表明,经过微波等离子体处理过的Ni-W、Ni-W-B非晶态合金镀层,提高了在H₂SO₄、HNO₃溶液中的耐蚀性以及在700℃环境下的抗氧化性能;微波等离子体处理过的非晶态合金镀层表面含W量和含O量相对提高了;镀层结构仍然是非晶态.     

仿壁虎刚毛阵列对卫星表面吸附能力模型与计算

壁虎脚的粘着机理对航天机器人脚掌的研制和开发具有重要启发意义.通过单根刚毛与光滑表面黏着力模型与计算、卫星粗糙表面分布模型与接触概率与计算、刚毛阵列与卫星粗糙表面黏着力模型与计算等,分析仿壁虎刚毛阵列对卫星表面的吸附能力,发现在一定条件下仿壁虎刚毛阵能够在太空失重环境中黏着在卫星表面,对航天舱内爬行机器人、管道机器人的...     

密闭微环境突发污染源动态散发特性辨识

载人航天器、飞机、潜艇等密闭微环境,随着人员停留时间的延长,舱室空气污染问题已成为危害工作人员生命安全的主要因素.为了提高上述密闭环境主动应对突发污染的能力,建立一种新的浓度离散随机模型,提出采用敏感性分析算法实现污染源定位及强度估计,利用隐式与显式卡尔曼滤波相结合的方法同时完成污染源散发特性的动态辨识及舱室空气污染物的浓度预测;分析了不同位置处的传感器可辨识区域,给出最优传感器布置策略.仿真结果证实了敏感性分析算法及隐式与显式卡尔曼滤波相结合方法能够实现污染源散发特性的快速准确辨识.     

天基朗缪尔探针传感器设计与仿真

朗缪尔探针是进行空间等离子体电子密度和温度就位测量的重要仪器, 在电离层卫星探测和火箭探测中应用十分广泛. 传感器设计的合理与否是影响朗缪尔探针探测结果的关键因素之一. 朗缪尔探针传感器的结构本身比较简单, 但传感器的设计要受到多种物理条件及工程条件的限制, 其中, 有些限制条件相互制约甚至相互矛盾, 必须综合进行考虑. 从传感器形状、大小和表面材料的选取三个方面分析了朗缪尔探针的各种限制条件, 给出了一种原型朗缪尔探针的设计, 并通过仿真计算验证了该设计.      

Plants + soil/wetland microbes: Food crop systems that also clean air and water

The limitations that will govern bioregenerative life support applications in space, especially volume and weight, make multi-purpose systems advantageous. This paper outlines two systems which utilize plants and associated microbial communities of root or growth medium to both produce food crops and clean air and water. Underlying these approaches are the large numbers and metabolic diversity of microbes associated with roots and found in either soil or other suitable growth media. Biogeochemical cycles have microbial links and the ability of microbes to metabolize virtually all trace gases, whether of technogenic or biogenic origin, has long been established. Wetland plants and the rootzone microbes of wetland soils/media also been extensively researched for their ability to purify wastewaters of a great number of potential water pollutants, from nutrients like N and P, to heavy metals and a range of complex industrial pollutants. There is a growing body of research on the ability of higher plants to purify air and water. Associated benefits of these approaches is that by utilizing natural ecological processes, the cleansing of air and water can be done with little or no energy inputs. Soil and rootzone microorganisms respond to changing pollutant types by an increase of the types of organisms with the capacity to use these compounds. Thus living systems have an adaptive capacity as long as the starting populations are sufficiently diverse. Tightly sealed environments, from office buildings to spacecraft, can have hundreds or even thousands of potential air pollutants, depending on the materials and equipment enclosed. Human waste products carry a plethora of microbes which are readily used in the process of converting its organic load to forms that can be utilized by green plants. Having endogenous means of responding to changing air and water quality conditions represents safety factors as these systems operate without the need for human intervention. We review this research and the ability of systems using these mechanisms to also produce food or other useful crops. Concerns about possible pathogens in soils and wastewater are discussed along with some methods to prevent contact, disease transmission and to pre-screen and decrease risks. The psychological benefits of having systems utilizing green plants are becoming more widely recognized. Some recent applications extending the benefits of plants and microbes to solve new environmental problems are presented. For space applications, we discuss the use of in situ space resources and ways of making these systems compact and light-weight.     

Changes in symbiotic and associative interrelations in a higher plant-bacterial system during space flight.

The miniature cenosis consisting of the water fern Azolla with its associated symbiotic nitrogen-fixing cyanobacterium Anabaena and the concomitant bacteria was investigated. Ecological closure was shown to produce sharp quantitative and qualitative changes in the number and type of concomitant bacteria. Changes in the distribution of bacterial types grown on beef-extract broth after space flight were recorded. Anabaena azollae underwent the most significant changes under spaceflight conditions. Its cell number per Azolla biomass unit increased substantially. Thus closure of cenosis resulted in a weakening of control over microbial development by Azolla. This tendency was augmented by spaceflight factors. Reduction in control exerted by macro-organisms over development of associated micro-organisms must be taken into account in constructing closed ecological systems in the state of weightlessness.     

Instrumentation for plant health and growth in space.

The present-day plant growth facilities ("greenhouses") for space should be equipped with monitors and controllers of ambient parameters within the chamber because spacecraft environmental variations can be unfavorable to plants. Moreover, little is known about the effects of spaceflight on the greenhouse and rooting media. Lack of information about spaceflight effects on plants necessitates supplying space greenhouses with automatic, non-invasive monitors of, e.g., gas exchange rate, water and nutrient ion uptake, plant mass, temperature and water content of leaves. However, introduction of an environmental or plant sensor into the monitoring system may be reasonable only if it is justified by quantitative evaluation of the influence of a measured parameter on productivity, efficacy of illumination, or some other index of greenhouse efficiency. The multivariate adaptive optimization in terrestrial phytotrons appears to be one of the best methods to assess environmental impacts on crops. Two modifications of greenhouses with the three-dimensional adaptive optimization of crop photosynthetic characteristics include: (1) irradiation, air temperature and carbon dioxide using a modified simplex algorithm; and (2) using irradiation, air temperature, and humidity with sensitivity algorithms with varying frequency of test exposures that have been experimentally developed. As a result, during some stages of plant ontogensis, the photosynthetic productivity of wheat, tomatoes, and Chinese cabbage in these systems was found to increase by a factor of 2-3.     

空间微生物实验技术研究进展

微生物具有结构简单、生长周期短、繁殖快、便于搭载等优点,被作为生物模型用于空间环境下的生命现象研究、地外生命探测以及开展以微生物为重点关注对象的行星保护任务研究.随着中国空间站在轨建造任务的临近,微生物空间实验技术开发以及相应的实验装置研制需要加快部署.本文以已实现空间应用的微生物技术为基础,从微生物空间培养、保存、检...     

中国载人航天工程的外部设计

在“神舟号”载人飞船工程实现了中国人往返于天地间的目的之后 ,中国应审慎地选择发展载人航天的目标。文章从中国社会对载人航天的需求出发 ,讨论了以开发利用空间微重力物质环境为目标的空间站和以发展天基航天为目标的天基航天站的外部工程系统的环境条件 ,认为中国在运载火箭、发射和回收场、测控站网方面已有较好基础 ,基本具备条件 ,运人运输器已有“神舟号”载人飞船 ,运物运输器的研制也不困难 ,但在为保障航天员在空间生活、工作的航天员系统方面和为实现载人航天工程功能和显现价值的有效载荷系统方面欠缺较多 ,需要一个研究、试验、培训和开发、演示的发展阶段     

CCD器件用机械泵驱动两相流体回路仿真与试验

电荷耦合元件（CCD）作为航天光学遥感器的核心部件之一,其工作性能受温度影响很大,传统的热控产品难以满足大功率CCD的精密控温需求。通过仿真与试验系统研究了机械泵驱动两相流体回路（MPTL）用于CCD控温时的启动特性、运行状态、内部工质的流动及传热特性。结果表明:MPTL可以通过干度的调节来吸收冷凝器外热流和CCD工作模式的影响;MPTL的控温精度可以达到±1℃,蒸发器并联支路、蒸发器负载和冷凝器温度在一定范围内变化等均不会对系统运行稳定性产生影响,其仍可将CCD器件控制在所需温度;通过仿真与试验对比,发现仿真模型的误差在±1℃以内,验证了模型的有效性和准确度。MPTL可以很好地满足航天光学遥感器CCD的控温要求,能够保证CCD始终具有较好的温度稳定性和均匀性,且系统具有良好的运行特性和鲁棒性,其在CCD精密控温方面具有很好的应用前景。