The Antaeus project: An orbital quarantine facility for analysis of planetary return samples

A systems design study group jointly sponsored by the American Society for Engineering Education, Stanford University and NASA's Ames Research Center, was requested to develop a design for an orbiting quarantine facility. The proposed facility is constructed of Spacelab shells formed into five modules of different sizes, each compatible with missions of other objectives. Once placed in a low Earth orbit by the Space Shuttle, each component is linked via the international docking system. Radiating from the docking module are a replaceable logistics module which stores a thirty day supply of consumables and waste, a module providing living quarters for five crew members, a power system module, and a quarantine testing laboratory. Within the laboratory module is a primary barrier system of sealed cabinets in which the sample is assessed for life forms. These chambers isolate the sample from terrestrial contamination and protect the researchers. A combination of procedures and mechanisms separates the laboratory module from the remainder of the facility and provides a secondary barrier. The conditions of space provide a tertiary barrier protecting the Earth's biosphere.     

Modern aspects of planetary protection and requirements to sterilization of space hardware.

The viewpoint of working group of Russian experts on the problem of planetary protection for future manned and unmanned Mars mission is presented. Recent data of Martian environment and on survival of terrestrial microorganisms in extreme conditions were used for detailed analysis and overview of planetary protection measures in regard to all possible flight situations including accidental landing. The special emphasis on "Mars-94" mission was done. This analysis resulted in revised formulation of spacecraft sterilization requirements and possible measures for their best implementation. New general combined approach to spacecraft sterilization was proposed. It includes penetrating radiation and heat treatment of spacecraft parts and components which is to be carried out before the final assembly of spacecraft and gaseous radiation sterilization of the whole spacecraft during the flight to Mars (or from Mars for return missions).     

Isotopic ratios in planetary atmospheres.

Recent progress on measurements of isotopic ratios in planetary or satellite atmospheres include measurements of the D/H ratio in the methane of Uranus, Neptune and Titan and in the water of Mars and Venus. Implications of these measurements on our understanding of the formation and evolution of the planets and satellite are discussed. Our current knowledge of the carbon, nitrogen and oxygen isotopic ratios in the atmospheres of these planets, as well as on Jupiter and Saturn, is also reviewed. We finally show what progress can be expected in the very near future due to some new ground-based instrumentation particularly well suited to such studies, and to forthcoming space missions.     

The pleurodele, an animal model for space biology studies.

Pleurodeles waltl, an Urodele amphibian is proposed as a model for space biology studies. Our laboratory is developing three types of experiments in space using this animal: 1) in vivo fertilization and development ("FERTILE" project); 2) influence of microgravity and space radiation on the organization and preservation of specialized structures in the neurons and muscle cells (in vitro; "CELIMENE" PROJECT); 3) influence of microgravity on tissue regeneration (muscle, bone, epidermis and spinal cord).     

Adaptive response studies may help choose astronauts for long-term space travel.

Long-term manned exploratory missions are planned for the future. Exposure to high-energy neutrons, protons and high charge and energy particles during a deep space mission, needs protection against the detrimental effects of space radiation. It has been suggested that exposure to unpredictable extremely large solar particle events would kill the astronauts without massive shielding. To reduce this risk to astronauts and to minimize the need for shielding, astronauts with highest significant adaptive responses should be chosen. It has been demonstrated that some humans living in very high natural radiation areas have acquired high adaptive responses to external radiation. Therefore, we suggest that for a deep space mission the adaptive response of all potential crew members be measured and only those with high adaptive response be chosen. We also proclaim that chronic exposure to elevated levels of radiation can considerably decrease radiation susceptibility and better protect astronauts against the unpredictable exposure to sudden and dramatic increase in flux due to solar flares and coronal mass ejections.     

Fullerenes in space.

The discovery and synthesis of fullerenes led to the hypothesis that they may be present and stable in interstellar space. Fullerenes have been reported in an impact crater on the LDEF spacecraft. Investigations of fullerenes in carbonaceous meteorites have yielded only small upper limits. Fullerene compounds and their ions could be interesting carrier molecules for some of the "diffuse interstellar bands" (DIBs), a long standing mystery in astronomy. We have detected two new diffuse bands that are consistent with laboratory measurements of the C60+, as first evidence for the largest molecule ever detected in space. Criteria for this identification are discussed. The inferred abundance (up to 0.9 % of cosmic carbon locked in C60+) suggests that fullerenes may play an important role in interstellar chemistry. We present new observations on DIB substructures consistent with fullerene compounds, and the search for neutral C60 in the diffuse medium.     

U.S. planetary protection program: Implementation highlights

The implementation of planetary protection in the United States space program has reflected the trend in policy from an absolute to a probabilistic prohibition of the contamination of the celestial bodies of the solar system. The early emphasis on spacecraft sterilization (e.g. Ranger) was replaced by the imposition of contamination control procedures on later missions such as Pioneer, Viking, and Voyager. Similarly, analytical and laboratory techniques were developed to demonstrate compliance with probabilistic requirements. Microbial burden reduction methods that are not hazardous for spacecraft reliability supplanted the abstract concept of sterilization. The United States implementation of planetary protection has been completely successful. In an exploration program that has included Mercury, Venus, Mars, the Jovian system, and the Saturnian system, there have been no accidental impacts or detection of false positives (terrestrial microbes). Further, the contamination control and microbial burden procedures have proved beneficial to spacecraft systems and on-board science instruments. We review in this paper the implementation of planetary protection procedures by the Pioneer (10 and 11), Viking and Voyager projects.     

Limits of photosynthesis in extrasolar planetary systems for earth-like planets.

We present a general modeling scheme for investigating the possibility of photosynthesis-based life on extrasolar planets. The scheme focuses on the identification of the habitable zone in main-sequence-star planetary systems with planets of Earth mass and size. Our definition of habitability is based on the long-term possibility of photosynthetic biomass production as a function of mean planetary surface temperature and atmospheric CO2-content. All the astrophysical, climatological, biogeochemical, and geodynamic key processes involved in the generation of photosynthesis-driven life conditions are taken into account. Implicitly, a co-genetic origin of the central star and the orbiting planet is assumed. The numerical solution of an advanced geodynamic model yields realistic look-up diagrams for determining the limits of photosynthesis in extrasolar planetary systems, assuming minimum CO2 levels set by the demand of C4 photosynthesis.     

Formation of bioorganic compounds in planetary atmospheres by cosmic radiation.

Simulated planetary atmospheres (mixtures of simple gases) were irradiated with high energy particles to simulate an action of cosmic rays. When a mixture of carbon monoxide, nitrogen and water was irradiated with 2.8-40 MeV protons, a wide variety of bioorganic compounds including amino acids, imidazole, and uracil were identified in the products. The amount of amino acids was proportional to the energy deposit to the system. Various kinds of simulated planetary atmospheres, such as "Titan type" and "Jovian type", were also irradiated with high energy protons, and gave amino acids in the hydrolyzed products. Since cosmic rays are a universal energy source in space, it was suggested that formation of bioorganic compounds in planetary atmospheres is inevitable in the course of cosmic evolution.     

Fundamental study on gas monitoring in CELSS.

A mass spectrometer and computer system was developed for conducting a fundamental study on gas monitoring in CELSS. Respiration and metabolism of the hamster and photosynthesis of the Spirulina were measured in a combination system consisting of a hamster chamber and a Spirulina cultivator. They are connected through a membrane gas exchanger. Some technical problems were examined. In the mass spectrometric gas monitoring, a simultaneous multi-sample measurement was developed by employing a rotating exchange valve. Long term precise measurement was obtained by employing an automatic calibration system. The membrane gas sampling probe proved to be useful for long term measurement. The cultivation rate of the Spirulina was effectively changed by controlling CO2 and light supply. The experimental results are helpful for improving the hamster-spirulina system.     

Implementing planetary protection requirements for sample return missions.

NASA is committed to exploring space while avoiding the biological contamination of other solar system bodies and protecting the Earth against potential harm from materials returned from space. NASA's planetary protection program evaluates missions (with external advice from the US National Research Council and others) and imposes particular constraints on individual missions to achieve these objectives. In 1997 the National Research Council's Space Studies Board published the report, Mars Sample Return: Issues and Recommendations, which reported advice to NASA on Mars sample return missions, complementing their 1992 report, The Biological Contamination of Mars Issues and Recommendations. Meanwhile, NASA has requested a new Space Studies Board study to address sample returns from bodies other than Mars. This study recognizes the variety of worlds that have been opened up to NASA and its partners by small, relatively inexpensive, missions of the Discovery class, as well as the reshaping of our ideas about life in the solar system that have been occasioned by the Galileo spacecraft's discovery that an ocean under the ice on Jupiter's moon Europa might, indeed, exist. This paper will report on NASA's planned implementation of planetary protection provisions based on these recent National Research Council recommendations, and will suggest measures for incorporation in the planetary protection policy of COSPAR.     

Pituitary cells in space.

Cells of the mammalian pituitary gland synthesize and secrete several protein hormones which regulate a number of organ systems throughout the body. These include the musculoskeletal, immune, vascular and endocrine systems. Since changes occur in these tissues as a result of spaceflight, and since pituitary growth hormone (GH) and prolactin (PRL) play a role in the control of these systems on earth, we have focused attention over the last 10 years on GH and PRL cell function during and after spaceflight. The cumulative results of 4 spaceflight missions and several mimicked microgravity experiments establish 1) that production and release of biologically active GH and PRL is repeatedly and significantly attenuated (usually > 50%) and 2) that changes in cell morphology also occur. In this paper we describe our results within the framework of methodologies and approaches frequently used to study pituitary cell function on earth. In so doing we hope to develop future flight experiments aimed at uncovering possible microgravity "sensing systems" within the pituitary cell.     

Legal aspects of planetary protection for Mars missions.

The planning and execution of manned and robotic missions to Mars present a wide range of jurisprudential issues. Provisions to prevent the disruption of natural celestial environments, as well as damage to the environment of Earth by the return of extraterrestrial materials, are important components of the law applicable to mankind's activities in outer space, and have been supplemented by scientifically instituted planetary protection policies. However, divergent legal regimes may exist, as the space treaties in force are neither uniform in their provisions, nor identical as to the states which have signed, ratified, or adopted the international agreements. The legal requirements applicable to a specific mission will vary depending on the entities conducting the program and specific mission profile. This article analyzes the divergent international legal regimes together with the factors which will influence the determination of the standards of conduct which will govern manned and robotic missions to Mars.     

Selection of sterilization methods for planetary return missions.

Two tasks must be accomplished to provide planetary protection for Mars return missions: (1) sterilization of the scientific module to be landed on Mars and (2) reliable sterilization of all material returned to Earth, while ensuring the scientific integrity of martian samples. This paper examines similarity and differences between these two tasks, and includes a discussion of technological implementation conditions and the nature of terrestrial and hypothesized martian microflora. The feasibility of a number of chemical and physical (ultraviolet and ionizing radiation and heating) methods of sterilization for use on the ground and onboard are discussed and compared. A combination of different methods will probably be selected as the most appropriate for ensuring planetary protection on the return mission.     

Designing planetary protection into the Mars Observer mission.

Planetary protection has been an important consideration during the process of designing the Mars Observer mission. It affected trajectory design of both the interplanetary transfer and the orbits at Mars; these in turn affected the observation strategies developed for the mission. The Project relied mainly on the strategy of collision avoidance to prevent contamination of Mars. Conservative estimates of spacecraft reliability and Martian atmosphere density were used to evaluate decisions concerning the interplanetary trajectory, the orbit insertion phase at Mars, and operations in orbit at Mars and afterwards. Changes in the trajectory design, especially in the orbit insertion phase, required a refinement of those estimates.     

Comets in other planetary systems?

Comets in our solar system appear to have provided a bridge between the cold, volatile-rich outer solar system, and the warm, but volatile-poor inner solar system. Excluding tidal and possible extinct radionuclide heating sources, only in the inner solar system are temperatures high enough for liquid water, and therefore life as we know it, to exist for times comparable to the age of the solar system. Comets may have been crucial for providing biogenic volatiles and perhaps organic molecules to this warm environment. It is therefore interesting from an exobiological point of view to ask if comets exist in other planetary systems. Most attempts to detect comets around other stars or in interstellar space have failed. However, there is growing spectroscopic evidence for comet-like bodies orbiting the star Beta Pictoris.     

Theoretical studies on the space CIV experiment over China

Recently the Active Experiment Working Group, Center for Space Science and Applied Research, has proposed a chemical release experiment program. The initial scientific object of the program is to study the critical ionization velocity (CIV) mechanism at low and middle latitudes over China. Beside of the development of the chemical module system, theoretical studies on the CIV phenomena of barium or strontium released over China are studied. We found that the angle between the release velocity and the ambient magnetic field over China is smaller than that in the same latitude region in North America and is advantageous for studying CIV phenomena and the evolution of ionized cloud along the magnetic lines. We also used a numerical simulation to simulate the evolution of chemical release clouds under various release conditions. For 1 kg barium and V-B angle equal to 60 degrees, we have a better configuration of ions cloud which can easily distinguish the ions created by CIV phenomena from that by the UV of the sunlight.     

Closed Artificial ecosystems as a means of ecosystem studies for Earth and space needs.

Closed Artificial ecosystems (CAES) have good prospects for wide use as new means for quantitative studies of different types of both natural ecosystems and man-made ones. The paper deals with the discussion of three points of CAES applications. The first one is of importance for theoretical ecology development and is connected with bringing together "holistic" and "merological" approaches in ecosystems studies. Using CAES, we can combine both approaches, taking into account the biotic turnover of limiting substrates which few in number even for complicated natural ecosystems. The second CAES use concerns the development of "ecosystems health" concept and application of a key-factor-approach for the indication and measurement of healthy unhealthy state and functioning of ecosystems or their links. The third use is more of an applied nature, oriented to the intensification of bioremediation or biodepollution processes in different types of ecosystems, including the global biosphere. Grant numbers: N 99-04-96017, N25.     

Mammalian cell cultivation in space.

Equipment used in space for the cultivation of mammalian cells does not meet the usual standard of earth bound bioreactors. Thus, the development of a space worthy bioreactor is mandatory for two reasons: First, to investigate the effect on single cells of the space environment in general and microgravity conditions in particular, and second, to provide researchers on long term missions and the Space Station with cell material. However, expertise for this venture is not at hand. A small and simple device for animal cell culture experiments aboard Spacelab (Dynamic Cell Culture System; DCCS) was developed. It provides 2 cell culture chambers, one is operated as a batch system, the other one as a perfusion system. The cell chambers have a volume of 200 microliters. Medium exchange is achieved with an automatic osmotic pump. The system is neither mechanically stirred nor equipped with sensors. Oxygen for cell growth is provided by a gas chamber that is adjacent to the cell chambers. The oxygen gradient produced by the growing cells serves to maintain the oxygen influx by diffusion. Hamster kidney cells growing on microcarriers were used to test the biological performance of the DCCS. On ground tests suggest that this system is feasible.     

Human reproductive issues in space.

Animal studies in space or analogous environments have suggested that there may be problems in the reproductive sphere; such factors might limit mankind's ability to live and work for extended periods of time in microgravity or on non-terrestrial planetary surfaces. A review of reproductive functioning in animal species studied during space flight demonstrated that most species were affected significantly by the absence of gravity and/or the presence of radiation. These two factors induced alterations in normal reproductive functioning independently of, as well as in combination with, each other. Based on animal models, we have identified several potential problem areas regarding human reproductive physiology and functioning in the space environment. While there are no current space flight investigations, the animal studies suggest priorities for future research in human reproduction. Such studies will be critical for the successful colonization of the space frontier.