Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa |
| |
Authors: | Yuan Xiao Yongding Liu Gaohong Wang |
| |
Institution: | 1. State Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, 7 South Donghu Road, Wuhan 430072, Hubei Province, PR China;2. Graduate School of Chinese Academy of Sciences, Beijing 100039, PR China |
| |
Abstract: | Simulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa. |
| |
Keywords: | SMG simulated microgravity M aeruginosa Microcystis aeruginosa MC microcystin NO nitric oxide SNP sodium nitroprusside c-PTIO 2-(4-carboxyphenyl)-4 4 5 5-tetramethylimidazoline-1-oxyl-3-oxide Chl a chlorophyll a PS II photochemical system II NOS nitric oxide synthase iNOS inducible nitric oxide synthase NR nitrate reductase ROS reactive oxygen species |
本文献已被 ScienceDirect 等数据库收录! |
|