微重力对拟南芥长势影响的代谢网络流平衡分析

微重力作为典型的空间环境因素，对植物生长发育的影响机制是空间生命科学的研究热点。微重力环境直接或间接影响植物代谢，并引起许多生理适应。 随着系统生物学的发展，代谢网络模型使微重力环境下的植物代谢建模成为可能。采用流平衡分析方法对模式植物拟南芥不同组织的代谢网络进行分析，研究微重力对拟南芥生长发育的影响机制。通过比较空间与地面条件下拟南芥的生物质产量，发现空间条件下拟南芥黄化幼苗、幼苗、芽、根、下胚轴的生物量分别下降了33.00%，51.52%，6.89%，12.53%，11.70%，与空间环境下拟南芥的长势变化趋势一致。代谢通路富集分析发现，微重力使得拟南芥的碳固定等通路下调，而磷酸戊糖途径上调，初步解析了微重力对拟南芥生长发育的影响机制，也验证了流平衡方法用于微重力生物学效应研究中的可行性。 

Metabolic Network Flux Balance Analysis of the Microgravity Influence on the Growth of Arabidopsis Thaliana

As a typical space environmental factor, the influence mechanism of microgravity on plants is a research hotspot in space life science. The microgravity environment directly or indirectly affects plant metabolism and induces many physiological adaptations. With the development of systems biology, the metabolic network model enables the modeling of plant metabolism in microgravity. In this study, the flux balance analysis method was used to study the metabolic networks of different tissues of the model plant Arabidopsis thaliana, and to explore the mechanism of the effect of microgravity on the growth and development of Arabidopsis thaliana. By comparing the biomass yield of Arabidopsis thaliana under space and ground conditions, it was found that the biomass of etiolated seedlings, seedlings, shoots, roots, and hypocotyls is decreased by 33.00%, 51.52%, 6.89%, 12.53%, and 11.70%, respectively, consistent with the growth trend of Arabidopsis thaliana in the space environment. Enrichment analysis of metabolic pathways showed that microgravity down-regulated Arabidopsis thaliana’s carbon fixation pathway, while up-regulated the pentose phosphate pathway. This result preliminarily analyzed the mechanism of microgravity influence on the growth and development of Arabidopsis thaliana and demonstrated the potential of the flux balance analysis method in the study of the biological effects of microgravity.