| 杂质对溶菌酶晶体表面形貌和生长动力学的影响 |
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| 引用本文： | 戴国亮,刘兴宇,王素静,康琦,胡文瑞.杂质对溶菌酶晶体表面形貌和生长动力学的影响[J].空间科学学报,2009,29(1). |
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| 作者姓名： | 戴国亮 刘兴宇 王素静 康琦 胡文瑞 |
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| 作者单位： | 中国科学院力学研究所,国家微重力实验室,北京,100190 |
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| 摘 要： | 采用相差显微镜研究了两种杂质蛋白(荧光标记的溶菌酶和商品溶菌酶中的杂质)对四方溶菌酶晶体表面形貌、{110}和{101}表面生长速度以及{110}面台阶生长动力学的影响.杂质会影响晶体表面二维生长岛的形貌,并显著降低晶体{110}和{101}面法向生长速度.杂质会显著降低{110}表面台阶的生长速度,且对<110>方向速度的抑制效果显著强于<001>方向.根据切向台阶速度和过饱和度的关系,计算得到了台阶动力学系数β和台阶的有效表面能及棱边自由能.
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| 关 键 词： | 蛋白质结晶 杂质 溶菌酶 |
Influence of Impurity on Surface Morphology and Growth Kinetics of a Lysozyme Crystal |
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| DAI Guoliang,LIU Xingyu,WANG Sujing,KANG Qi,HU Wenrui.Influence of Impurity on Surface Morphology and Growth Kinetics of a Lysozyme Crystal[J].Chinese Journal of Space Science,2009,29(1). |
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| Authors: | DAI Guoliang LIU Xingyu WANG Sujing KANG Qi HU Wenrui |
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| Institution: | National Mierogravity Laboratory;Institute of Mechanics;Chinese Academy of Sciences;Beijing 100190 |
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| Abstract: | The influences of impurity on snrface morphology and growth kinetics of tetragonal lysozyme crystals were studied.There was a significant difference in morphology of 2D islands on a {110} face when the impurities existed.Impurities significantly decreased the normal growth rates of both the {110} and {101} face.F-lysozyme decreased the normal growth rate of both the {110} face and {101} face.The values of kinetic coefficient of the steps,surface free energy,and ledge free energy of the step edge were determ... |
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| Keywords: | Protein crystallization Impurity Lysozyme |
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