纳米二氧化钛吸入对小鼠肺部和血清生化指标的影响

将成年健康的小鼠(每组8只)暴露在含有1 500 mg/m3纳米二氧化钛气溶胶密闭装置内,以暴露在空气中的小鼠作为对照,研究了纳米二氧化钛对小鼠肺部组织和血清生化指标的影响.经过不同时间的暴露后将小鼠处死,收集血样并取肺部组织进行生化指标分析和组织病理学检验.血清生化指标检测结果显示,在所有的试验组中,乳酸脱氢酶的活性均提高,丙氨酸氨基转移酶的活性和尿素氮在暴露28天组中增加,肌酐在暴露14天组和28天组中均增加,其他血清学指标未见明显异常.组织病理学检测结果显示,暴露28天的肺泡间隙有纳米粒子存在,并且肺部炎症逐渐增强.

EFFECT OF INHALED NANO-TiO2 ON LUNG AND SERUM BIOCHEMICAL INDEXES OF MICE

The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice (eight in each group) after exposure to TiO2 aerosols (1 500 mg/m3) in a sealed chamber. Another eight mice are exposed to indoor air to be served as a control group. Lung tissue and blood are collected after euthanizing the animals. The results show that lactate dehydrogenase (LDH) activity increases in all experimental groups. Alanine aminotransferase(ALT) activity and blood urea nitrogen (BUN) levels are increased in the group exposed to TiO2 aerosols for 28 d, and creatinine(Cr) levels are increased in 14 d and 28 d samples (P＜0.05). No obvious changes are observed in other serum indexes. Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation, and many TiO2 particles are found in the interstitium of pulmonary alveoli.