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| 应用绿色半导体量子点进行乳腺癌原位成像 | |
| 引用本文： | 许恒毅,苏怀朋,John.D.Dixon,熊勇华,魏华,Andrew Y.Wang.应用绿色半导体量子点进行乳腺癌原位成像[J].南京航空航天大学学报(英文版),2010,27(1):13-20. |
| 作者姓名： | 许恒毅 苏怀朋 John.D.Dixon 熊勇华 魏华 Andrew Y.Wang |
| 作者单位： | 1. 南昌大学食品科学与技术国家重点实验室,南昌,330047,中国;Ocean NanoTech LLC,2143 Worth Lane.Springdale,72764,USA 2. Ocean NanoTech LLC,2143 Worth Lane.Springdale,72764,USA 3. 南昌大学食品科学与技术国家重点实验室,南昌,330047,中国 |
| 基金项目： | Supported by the Foundation for Cultivating the Excellent Doctoral Dissertation of Jiangxi Province of China (YBP08A03)~~ |
| 摘 要： | 乳腺癌是癌症中引起妇女死亡的首要因素。本文采用绿色量子点作为荧光显色材料，建立了一种用于早期乳腺癌诊断的原位成像技术。利用生物素标记的抗HER2／neu抗体与乳腺癌细胞表面表达的HER2结合的特点，然后用偶联绿色量子点的链霉亲和素作为荧光检测标记物，与HER2抗原抗体复合物中的生物素发生特异结合，检测出乳腺癌细胞的存在。结果表明，在荧光显微镜下用绿色量子点原位显色的乳腺癌细胞，相对有机染料组显色亮度增强，故检测灵敏度有所提高。 |
| 关 键 词： | 原位处理 量子点 乳腺癌细胞 非特异性吸附 免疫组化染色 |
IN SITU IMAGING OF BREAST CANCER CELLS USING GREEN SEMICONDUCTOR QUANTUM DOTS |
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| Zoraida P.Aguilar,Benjamin J.Jones,John.D.Dixon,Andrew Y.Wang.IN SITU IMAGING OF BREAST CANCER CELLS USING GREEN SEMICONDUCTOR QUANTUM DOTS[J].Transactions of Nanjing University of Aeronautics & Astronautics,2010,27(1):13-20. | |
| Authors: | Zoraida PAguilar Benjamin JJones JohnDDixon Andrew YWang |
| Institution: | 1.State Key Laboratory of Food Science and Technology/a>;Nanchang University/a>;Nanchang/a>;330047/a>;P.R.China/a>;2.Ocean NanoTech LLC/a>;2143 Worth Lane/a>;Springdale/a>;AR 72764/a>;USA |
| Abstract: | The breast cancer is the most common cause of cancer death in women.To establish an early stage in situ imaging of breast cancer cells,green quantum dots (QDs) are used as a fluorescent signal generator.The QDs based imaging of breast cancer cells involves anti-HER2/neu antibody for labeling the over expressed HER2 on the surface of breast cancer cells.The complete assay involves breast cancer cells,biotin labeled antibody and streptavidin conjugated QDs.The breast cancer cells are grown in culture plates a... |
| Keywords: | in situ processing quantum optics breast cancer cells non-specific binding immuno-histochemical imaging |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |