Assessment of plant vitality detection through fluorescence and reflectance imagery

Recent studies of the vegetation fluorescence show that it can be successfully used as an intrinsic indicator of plant photosynthetic activity. With respect to the vegetation spectral reflectance, the chlorophyll (Chl) fluorescence is more specific as an observable of basic biophysical processes in the plant cells. Laser induced fluorescence is widely used in near range remote sensing, but it is not suitable for the global monitoring of vegetation. Decades of active fluorometry studies have collected useful information of leaf reaction to natural and anthropogenic stress. Still the passive fluorescence, the one that could be registered from satellite orbit has still to prove its advantage over widely used reflectance signature. The weakness of the signal and the lack of experience with passive fluorescence measurements require extensive technical, theoretical and experimental studies. New imaging fluorometres are to be designed for measuring steady state fluorescence in controlled and natural conditions.

In order to compare reflectance and steady state fluorescence sensitivity to stress impact, a set of experiments have been conducted under controlled illumination conditions in a bio-chamber, designed by the author’s team. The equipment allows plant vitality to be monitored both by passive fluorescence and spectral reflectance imaging. Different types of stress factors (heat and drought stress, acid impact) were investigated to demonstrate equipments ability in monitoring changes of fluorescence signal. Selected fluorescence images of foliage illustrate an early detection of plant dysfunction and the temporal and spatial spreading of the stress impact. Analysis shows that fluorescence imaging of green plants can be developed as a highly effective early warning remote sensing method, which could have application for an ecosystems’ monitoring along with high-spectral reflectance imagery.