Blue light-dependent phosphorylations of cryptochromes are affected by magnetic fields in Arabidopsis

The blue light receptor cryptochrome that could form radical pairs after exposure to blue light was suggested to be a magnetoreceptor based on the proposition that radical pairs were involved in the magnetoreception. But the effects of magnetic fields on the function of cryptochrome are poorly understood. Phosphorylation of cryptochrome in Arabidopsis was closely associated with the function of this photoreceptor. Here, we grew Arabidopsis seedlings in a 500 μT magnetic field and a near-null magnetic field and found that the 500 μT magnetic field enhanced the blue light-dependent phosphorylations of CRY1 and CRY2, and the near-null magnetic field weakened the blue light-dependent phosphorylation of CRY2 but not CRY1. Dephosphorylations of CRY1 and CRY2 in the darkness were slowed down in the 500 μT magnetic field, whereas dephosphorylations of CRY1 and CRY2 were accelerated in the near-null magnetic field. These results suggest that magnetic field with strength higher or weaker than the local geomagnetic field affects the activated states of cryptochromes, which thus modifies the functions of cryptochromes.