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Non-cultural methods of human microflora evaluation for the benefit of crew medical control in confined habitat
Authors:Ilyin Viacheslav  Moukhamedieva Lana  Osipov Georgy  Batov Aleksey  Soloviova Zoya  Mardanov Robert  Panina Yana  Gegenava Anna
Institution:1. German Aerospace Center (DLR) Institute of Planetary Research, 12489 Berlin, Germany;2. LESIA/Observatoire de Paris, 92195 Meudon Cedex, France;3. Deimos Space, Tres Cantos, 28760 Madrid, Spain;4. Queen''s University Belfast, Belfast BT7 1NN, UK;5. Open University, Milton Keynes MK7 6AA, UK;6. IMCCE/Observatoire de Paris, 75014 Paris, France;7. University of Surrey, Guildford GU2 7XH, UK;8. EADS Astrium GmbH, 88090 Immenstaad, Germany;9. CNRS, Observatoire de la Côte d''Azur, 06304 Nice Cedex 4, France;10. SETI Institute, Carl Sagan Center, Mountain View, CA 94043, USA;11. TsNIIMash, Korolev 141070, Russia;12. Fraunhofer Ernst Mach Institute, 79104 Freiburg, Germany;1. Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea;2. Clean Fuel Department, Korea Institute of Energy Research, Daejeon 305-343, Republic of Korea;1. Tupolev Kazan National Research Technical University, Kazan Aviation Institute, 420111, Kazan, Tatarstan, Russia;2. Saint-Petersburg State University of Civil Aviation, 196210, Saint-Petersburg, Russia;3. Bauman Moscow State Technical University, 105005, Moscow, Russia;1. Dipartimento di Scienze Chimiche and INSTM UdR Università di Catania, Viale A. Doria, 6, 95100 Catania, Italy;2. Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria, 6, 95100 Catania, Italy;1. School of Instrumental Science and Opto-electronics Engineering, Beihang University, 100191, China;2. School of Mechanical Engineering and Applied Electronics, Beijing University of Technology, 101100, China;3. Shanghai Key Laboratory of Aerospace Intelligent Control Technology, Shanghai Aerospace Control Technology Institute, Shanghai, 201109, China;1. School of Aeronautics and Astronautics, Zhejiang University, Hangzhou 310027, Zhejiang, PR China;2. School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, UK;3. School of Engineering and Physical Sciences, Heriot-Watt University Malaysia, Putrajaya, 62200, Malaysia;4. Propulsion Unit, National Space Agency Malaysia (ANGKASA), Banting, 42700, Malaysia
Abstract:Current control of human microflora is a great problem not only for the space medicine but also for practical health care. Due to many reasons its realization by classical bacteriological method is difficult in practical application or cannot be done.To evaluate non-cultural methods of microbial control of crews in a confined habitat we evaluated two different methods.The first method is based on digital treatment of microbial visual images, appearing after gram staining of microbial material from natural sample. This way the rate between gram-positive and gram-negative microbe could be gained as well as differentiation of rods and cocci could be attained, which is necessary for primary evaluation of human microbial cenosis in remote confined habitats.The other non-culture method of human microflora evaluation is gas chromatomass spectrometry (gcms) analysis of swabs gathered from different body sites. Gc-ms testing of swabs allows one to validate quantitative and special microflora based on specific lipid markers analysis.
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