Gravity effects on connective tissue biosynthesis by cultured mesenchymal cells.

Quantitative and qualitative aspects of collagen synthesis under microgravity, normal gravity and hypergravity conditions were investigated during the spacelab D-2 mission by incubating human fibroblast cultures with [3H]-proline for 0, 4, 7, 10 and 20 hours. Quantitative analysis revealed an increase of collagen synthesis under microgravity conditions, being 40% higher than 1g controls. Hypergravity samples at 1.44g, 6.6g and 10g showed a decrease in collagen synthesis with increasing g, being down to about 15% at 10g. The relative proportion of collagen from total protein synthesized, the secretion of collagen by the cells, proline hydroxylation of individual collagen alpha-chains and the relative proportions of collagens I, III and V synthesized were not affected at any of the applied conditions.     

Spectroscopic Measurement of Coronal Compositions

Although the elemental composition in all parts of the solar photosphere appears to be the same this is clearly not the case with the solar upper atmosphere (SUA). Spectroscopic studies show that in the corona elemental composition along solar equatorial regions is usually different from polar regions; composition in quiet Sun regions is often different from coronal hole and active region compositions and the transition region composition is frequently different from the coronal composition along the same line of sight. In the following two issues are discussed. The first involves abundance ratios between the high-FIP O and Ne and the low-FIP Mg and Fe that are important for meaningful comparisons between photospheric and SUA compositions and the second involves a review of composition and time variability of SUA plasmas at heights of 1.0≤h≤1.5R _⊙.     

The Genesis Solar Wind Concentrator Target: Mass Fractionation Characterised by Neon Isotopes

The concentrator on Genesis provided samples of increased fluences of solar wind ions for precise determination of the oxygen isotopic composition. The concentration process caused mass fractionation as a function of the radial target position. This fractionation was measured using Ne released by UV laser ablation and compared with modelled Ne data, obtained from ion-trajectory simulations. Measured data show that the concentrator performed as expected and indicate a radially symmetric concentration process. Measured concentration factors are up to ∼30 at the target centre. The total range of isotopic fractionation along the target radius is 3.8%/amu, with monotonically decreasing ²⁰Ne/²²Ne towards the centre, which differs from model predictions. We discuss potential reasons and propose future attempts to overcome these disagreements.     

Space Shuttle UHF communications performance evaluation

Computational investigations and experimental measurements were performed to evaluate the Space Shuttle UHF communication system performance for payload bay antenna at the proposed new location. To insure adequate communication coverage at relocated new location, the link margin for the Extravehicular Activity (EVA) astronauts and between Space Shuttle Orbiter and International Space Station (SSO-ISS) during rendezvous were analyzed. The multipath effects from the vehicle structures surrounding the antenna were investigated. The Radio Frequency (RF) electromagnetic radiation to the Orbiter Docking System (ODS) pyrotechnics was also analyzed to ensure the EMC/EMI compliances.     

Road profile recognition for autonomous car navigation and Navstar GPS support

We discuss autonomous car navigation based on updating dead reckoning (DR) by road profile recognition (RPR). The navigation system requires sensors to detect changes in altitude and driving direction which are installed in modern cars for different purposes (e.g. ABS sensors). The layout of the navigation system is discussed and simulations are carried out over driving distances of approximately 150 km on the basis of realistic road data and ordinary sensor accuracies. Positioning errors of lower than 10 m (standard deviation) are observed. To achieve this accuracy the synchronization error between measured and mapped data must be continually estimated. The introduced navigation method is ideal to complete present commercial car navigation systems using Navstar GPS.     

Biological responses to space: results of the experiment "Exobiological Unit" of ERA on EURECA I.

Spores of different strains of Bacillus subtilis and the Escherichia coli plasmid pUC19 were exposed to selected conditions of space (space vacuum and/or defined wavebands and intensities of solar ultraviolet radiation) in the experiment ER 161 "Exobiological Unit" of the Exobiology Radiation Assembly (ERA) on board of the European Retrievable Carrier (EURECA). After the approximately 11 months lasting mission, their responses were studied in terms of survival, mutagenesis in the his (B. subtilis) or lac locus (pUC19), induction of DNA strand breaks, efficiency of DNA repair systems, and the role of external protective agents. The data were compared with those of a simultaneously running ground control experiment. The survival of spores treated with the vacuum of space, however shielded against solar radiation, is substantially increased, if they are exposed in multilayers and/or in the presence of glucose as protective, whereas all spores in "artificial meteorites", i.e. embedded in clays or simulated Martian soil, are killed. Vacuum treatment leads to an increase of mutation frequency in spores, but not in plasmid DNA. Extraterrestrial solar ultraviolet radiation is mutagenic, induces strand breaks in the DNA and reduces survival substantially; however, even at the highest fluences, i.e. 3 x 10(8) J m-2, a small but significant fraction of spores survives the insolation. Action spectroscopy confirms results of previous space experiments of a synergistic action of space vacuum and solar UV radiation with DNA being the critical target.     

Experimental Aspects of Mid-Frequency Pulsations (f≈10–100 mHz) in the Southern Polar Cap

We review the results obtained in the frequency range of Pc3 (22-100 mHz) and Pc4 (7-22 mHz) pulsations at Italian Antarctic stations in the southern polar cap (“Mario Zucchelli”, at Terra Nova Bay, TNB, 80^˚.S; “Concordia”, the Italian/French base at Dome C, DMC, 89^˚.S). The absence of a midnight enhancement in the pulsation power suggests a negligible substorm influence at extreme latitudes, while the sharp noon enhancement, which appears only at TNB, is determined by the closer proximity of the station to cusp related phenomena. The relationship between the frequency of the band-limited signals and the interplanetary magnetic field strength, the cone angle influence, and the higher correlation of the Pc3 power with the solar wind speed in the morning hours suggest a global scenario in which upstream waves would be mainly responsible for the mid-frequency activity in the polar cap. However, the polarization pattern is odd with respect to the predictions for tailward propagating modes.     

Summary of quantitative interpretation of IMAGE far ultraviolet auroral data

Direct imaging of the magnetosphere by instruments on the IMAGE spacecraft is supplemented by simultaneous observations of the global aurora in three far ultraviolet (FUV) wavelength bands. The purpose of the multi-wavelength imaging is to study the global auroral particle and energy input from the magnetosphere into the atmosphere. This paper describes the method for quantitative interpretation of FUV measurements. The Wide-Band Imaging Camera (WIC) provides broad band ultraviolet images of the aurora with maximum spatial resolution by imaging the nitrogen lines and bands between 140 and 180 nm wavelength. The Spectrographic Imager (SI), a dual wavelength monochromatic instrument, images both Doppler-shifted Lyman-α emissions produced by precipitating protons, in the SI-12 channel and OI 135.6 nm emissions in the SI-13 channel. From the SI-12 Doppler shifted Lyman-α images it is possible to obtain the precipitating proton flux provided assumptions are made regarding the mean energy of the protons. Knowledge of the proton (flux and energy) component allows the calculation of the contribution produced by protons in the WIC and SI-13 instruments. Comparison of the corrected WIC and SI-13 signals provides a measure of the electron mean energy, which can then be used to determine the electron energy flux. To accomplish this, reliable emission modeling and instrument calibrations are required. In-flight calibration using early-type stars was used to validate the pre-flight laboratory calibrations and determine long-term trends in sensitivity. In general, very reasonable agreement is found between in-situ measurements and remote quantitative determinations.     

Far ultraviolet imaging from the IMAGE spacecraft. 3. Spectral imaging of Lyman-α and OI 135.6 nm

Two FUV Spectral imaging instruments, the Spectrographic Imager (SI) and the Geocorona Photometer (GEO) provide IMAGE with simultaneous global maps of the hydrogen (121.8 nm) and oxygen 135.6 nm components of the terrestrial aurora and with observations of the three dimensional distribution of neutral hydrogen in the magnetosphere (121.6 nm). The SI is a novel instrument type, in which spectral separation and imaging functions are independent of each other. In this instrument, two-dimensional images are produced on two detectors, and the images are spectrally filtered by a spectrograph part of the instrument. One of the two detectors images the Doppler-shifted Lyman- while rejecting the geocoronal `cold Ly-, and another detector images the OI 135.6 nm emission. The spectrograph is an all-reflective Wadsworth configuration in which a grill arrangement is used to block most of the cold, un-Doppler-shifted geocoronal emission at 121.567 nm. The SI calibration established that the upper limit of transmission at cold geocoronal Ly- is less than 2%. The measured light collecting efficiency was 0.01 and 0.008 cm² at 121.8 and at 135.6 nm, respectively. This is consistent with the size of the input aperture, the optical transmission, and the photocathode efficiency. The expected sensitivity is 1.8×10^–2 and 1.3×10^–2 counts per Rayleigh per pixel for each 5 s viewing exposure per satellite revolution (120 s). The measured spatial resolution is better than the 128×128 pixel matrix over the 15°×15° field of view in both wavelength channels. The SI detectors are photon counting devices using the cross delay line principle. In each detector a triple stack microchannel plate (MCP) amplifies the photo-electronic charge which is then deposited on a specially configured anode array. The position of the photon event is measured by digitizing the time delay between the pulses detected at each end of the anode structures. This scheme is intrinsically faster than systems that use charge division and it has a further advantage that it saturates more gradually at high count rates. The geocoronal Ly- is measured by a three-channel photometer system (GEO) which is a separate instrument. Each photometer has a built in MgF₂ lens to restrict the field of view to one degree and a ceramic electron multiplier with a KBr photocathode. One of the tubes is pointing radially outward perpendicular to the axis of satellite rotation. The optic of the other two subtend 60° with the rotation axis. These instruments take data continuously at 3 samples per second and rely on the combination of satellite rotation and orbital motion to scan the hydrogen cloud surrounding the earth. The detective efficiencies (effective quantum efficiency including windows) of the three tubes at Ly- are between 6 and 10%.