Effect of clinostating on photosynthetic apparatus of pea plants.

The photosynthetic membrane composition and low temperature fluorescence spectra were analyzed for pea chloroplasts from control and clinostated plants. Clinorotation induces a decrease in the amount of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCII) and an increase of its monomeric form. Some changes in organization of photosystem 1 (PS1) complex were revealed as well. These changes are in accordance with the variations of fluorescence characteristics and photochemical activity.     

Organization of cytoskeleton during differentiation of gravisensitive root sites under clinorotation.

Key role in cell gravisensing is attributed to the actin cytoskeleton which acts as a mediator in signaling reactions, including graviperception. Despite of increased attention to the actin cytoskeleton, major gaps in our understanding of its functioning in plant gravisensing still remain. To fill these gaps, we propose a novel approach focused on the investigation of actin involvement in the development of columella cells and cells in the transition zone of roots submitted to clinorotation. Both statocytes and cells in the transition zone represent the postmitotic cells which take origin in root meristems and are specified into graviperceptive (root cap) and gravireacting (transition zone) root tissues. The aim of the research was to investigate and compare the microfilament arrangements in root cap statocytes and peripheral root tissues (epidermis and cortex cells) in the transition zone and to find out how the actin cytoskeleton is involved in their specification under clinostat conditions. So far, our experiments have shown that under clinorotation the cytoplasmic microfilament network in the cortex cells in the transition zone is significantly enhanced. It is suggested that more abundant cytoplasmic microfilaments could strengthen the cortical actin cytoskeleton arranged parallel with the cortical microtubules, which are found to be partially disorganized in this area. Due to microtubule disorganization, the functioning of cellulose-synthesizing machinery and proper deposition of cell wall might be affected and could cause the alterations in the growth mode. But, in our case growth of the cells in the transition zone under clinorotation was rather stable. Due to our opinion, general stability of cell growth under clinorotation is promoted by mutual functional interrelation between actin and tubulin cytoskeletons. It is suggested that a strengthened cortical actin cytoskeleton restricts the cell growth instead of disorganized microtubules.     

On the Possibility of the Existence of Volatile Compounds in the Region of the Scott Crater on the Moon

In this paper, we analyze the illumination conditions, the thermal regime, and the possibility of deposits of volatile compounds existing in the vicinity region (NSR S5 region) near the southern pole of the Moon. It has been found that there are no permanently shadowed zones near the Scott crater and the NSR S5 region, though the temperature conditions allow the of compounds such as CH₃OH, SO₂, NH₃, CO₂, H₂S, C₂H₄, and water to remain stable relative to evaporation for a long time (≥1 Gyr). It has been also shown that compounds like CO and CH₄ cannot stably exist in these regions.     

Lunar Exploration Neutron Detector for the NASA Lunar Reconnaissance Orbiter

The design of the Lunar Exploration Neutron Detector (LEND) experiment is presented, which was optimized to address several of the primary measurement requirements of NASA’s Lunar Reconnaissance Orbiter (LRO): high spatial resolution hydrogen mapping of the Moon’s upper-most surface, identification of putative deposits of appreciable near-surface water ice in the Moon’s polar cold traps, and characterization of the human-relevant space radiation environment in lunar orbit. A comprehensive program of LEND instrument physical calibrations is discussed and the baseline scenario of LEND observations from the primary LRO lunar orbit is presented. LEND data products will be useful for determining the next stages of the emerging global lunar exploration program, and they will facilitate the study of the physics of hydrogen implantation and diffusion in the regolith, test the presence of water ice deposits in lunar cold polar traps, and investigate the role of neutrons within the radiation environment of the shallow lunar surface.     

ALTEA: anomalous long term effects in astronauts. A probe on the influence of cosmic radiation and microgravity on the central nervous system during long flights.

The ALTEA project participates to the quest for increasing the safety of manned space flights. It addresses the problems related to possible functional damage to neural cells and circuits due to particle radiation in space environment. Specifically it aims at studying the functionality of the astronauts' Central Nervous Systems (CNS) during long space flights and relating it to the peculiar environments in space, with a particular focus on the particle flux impinging in the head. The project is a large international and multidisciplinary collaboration. Competences in particle physics, neurophysiology, psychophysiology, electronics, space environment, data analyses will work together to construct the fully integrated vision electrophysiology and particle analyser system which is the core device of the project: an helmet-shaped multi-sensor device that will measure concurrently the dynamics of the functional status of the visual system and passage of each particle through the brain within a pre-determined energy window. ALTEA is scheduled to fly in the International Space Station in late 2002. One part of the multi-sensor device, one of the advanced silicon telescopes, will be launched in the ISS in early 2002 and serve as test for the final device and as discriminating dosimeter for the particle fluences within the ISS.     

Remote estimation of the structure of the surface layer of Mercury

Results of the optical observations of Mercury and the Moon confirm the close similarity of photometric properties of the bodies. Undoubtedly, the surface of Mercury, which is subjected to the same processes, is covered by a mantle of shattered rocks – the regolith. The structure of the reflecting layer determines the photometric and polarization characteristics of the surface of a planetary body. Despite the general similarity of the integral optical properties of the surfaces of Mercury and the Moon, specific characteristics of the media of these celestial bodies manifest themselves as identifiable differences in the details of the measured parameters: Mercury’s regolith is smoother than of the Moon, probably contains a greater fines fraction, and has greater maturity.     

Cytogenetic studies of blood lymphocytes from cosmonauts after long-term space flights on Mir station.

Long-term space missions may increase risks of unfavorable consequences for cosmonauts as a result of radiation effects. This paper presents results of a study of cytogenetic damage in cosmonauts' peripheral blood lymphocytes induced by space radiation. Cultivation of lymphocytes and analysis of chromosomal aberrations were made according to generally accepted methods. It is shown that the yields of dicentrics and centric rings scored after long-term space flights are considerably higher than those scored prior to the flights. An attempt was made to assess individual doses received by cosmonauts. Individual biodosimetry doses received by cosmonauts who showed a reliable increase in the yields of chromosomal-type aberrations after their first flights were estimated to be from 0.02 to 0.28 Gy.     

Application of GFP technique for cytoskeleton visualization onboard the International Space Station

Cytoskeleton recently attracted wide attention of cell and molecular biologists due to its crucial role in gravity sensing and trunsduction. Most of cytoskeletal research is conducted by the means of immunohistochemical reactions, different modifications of which are beneficial for the ground-based experiments. But for the performance onboard the space vehicles, they represent quite complicated technique which requires time and special skills for astronauts. In addition, immunocytochemistry provides only static images of the cytoskeleton arrangement in fixed cells while its localization in living cells is needed for the better understanding of cytoskeletal function. In this connection, we propose a new approach for cytoskeletal visualization onboard the ISS, namely, application of green fluorescent protein (GFP) from Aequorea victoria, which has the unique properties as a marker for protein localization in vivo. The creation of chimerical protein-GFP gene constructs, obtaining the transformed plant cells possessed protein-GFP in their cytoskeletal composition will allow receiving a simple and efficient model for screening of the cytoskeleton functional status in microgravity.     

Dust hazard near Halley Comet in case of the VEGA project

The motion of dust particles near Halley Comet is studied and the probability of dust impacts with the spacecraft in case of the VEGA (Venus-Halley)- project is determined. The formation of a crater due to a particle impact with the dust shield is considered and the necessity for using a dual-sheet bumper shield is substantiated. The thickness of a front sheet that plays a role of the particle evaporator is estimated theoretically. The numerical experiment is carried out that simulates the dynamics of collision and evaporation of a particle. Three factors causing perforations of the rear sheet are discussed, i.e. dust penetrated through holes in the front sheet, gas jets and spall fragments of the front-sheet. The consideration of these factors makes it possible to estimate basic parameters of the dual-sheet bumper shield. Flexural vibrations of the front sheet under action of the reverse gaseous jet from the rear sheet are discussed that can affect essentially the shield strength. The perturbing effect of the dust and gas fluxes on the spacecraft is studied.     

俄罗斯“月球-全球”和“月球-资源”探月任务

俄罗斯月球-全球-1着陆器将于2015年发射,主要目的是测试月球着陆器的软着陆技术。月球-全球-2轨道器计划于2016年发射,将先后在距月面500km、150km和50km的高度上飞行。2017年计划发射的月球-资源-1着陆器,将是搭载大量科学载荷和承担相应探测任务的月面科学研究站。与苏联时期所研制的月球车相比,分别在月球-资源-2和月球-资源-3着陆器上搭载的俄罗斯新一代月球车——月球车-3和月球车-4,将携带更加先进的科学仪器,能够精确分析采集样品的化学成分、光谱特征等。其运行模式包括行走、勘察、接近目标、接触目标和样品采集与分析,将对月球极区表面环境进行全面探测。结合对俄罗斯"月球-全球"和"月球-资源"探月任务的介绍,针对中国的探月计划提出如下建议:探月任务的实施,应以科学研究为主要目的,以建立月球基地为目标,借鉴国外探月技术,开展国际合作,为形成独立的月球科学体系打下坚实基础。