In situ microbial detection in Mojave Desert soil using native fluorescence

We report on the use of a portable instrument for microbial detection in the Mojave Desert soil and the potential for its use on Mars. The instrument is based on native fluorescence and employs four excitation wavelengths combined with four emission wavelengths. A soil dilution series in which known numbers of Bacillus subtilis spores were added to soil was used to determine the sensitivity of the instrument. We found that the fluorescence of the biological and organic components of the desert soil samples studied can be as strong as the fluorescence of the mineral component of these soils. Using the calibration derived from B. subtilis spores, we estimated that microbial content at our primary sampling site was 10(7) bacteria per gram of soil, a level confirmed by phospholipid fatty acid analysis. At a nearby site, but in a slightly different geological setting, we tested the instrument's ability to map out microbial concentrations in situ. Over a ～50 m diameter circle, soil microbial concentrations determined with the B. subtilis calibration indicate that the concentrations of microorganisms detected varies from 10(4) to 10(7) cells per gram of soil. We conclude that fluorescence is a promising method for detecting soil microbes in noncontact applications in extreme environments on Earth and may have applications on future missions to Mars.     

Spaceflight-relevant types of ionizing radiation and cortical bone: Potential LET effect?

Extended exposure to microgravity conditions results in significant bone loss. Coupled with radiation exposure, this phenomenon may place astronauts at a greater risk for mission-critical fractures. In a previous study, we identified a profound and prolonged loss of trabecular bone (29–39%) in mice following exposure to an acute, 2 Gy dose of radiation simulating both solar and cosmic sources. However, because skeletal strength depends on trabecular and cortical bone, accurate assessment of strength requires analysis of both bone compartments. The objective of the present study was to examine various properties of cortical bone in mice following exposure to multiple types of spaceflight-relevant radiation. Nine-week old, female C57BL/6 mice were sacrificed 110 days after exposure to a single, whole body, 2 Gy dose of gamma, proton, carbon, or iron radiation. Femora were evaluated with biomechanical testing, microcomputed tomography, quantitative histomorphometry, percent mineral content, and micro-hardness analysis. Compared to non-irradiated controls, there were significant differences compared to carbon or iron radiation for only fracture force, medullary area and mineral content. A greater differential effect based on linear energy transfer (LET) level may be present: high-LET (carbon or iron) particle irradiation was associated with a decline in structural properties (maximum force, fracture force, medullary area, and cortical porosity) and mineral composition compared to low-LET radiation (gamma and proton). Bone loss following irradiation appears to be largely specific to trabecular bone and may indicate unique biological microenvironments and microdosimetry conditions. However, the limited time points examined and non-haversian skeletal structure of the mice employed highlight the need for further investigation.     

Future Directions in Automation and Robotics for Manufacturing

The current emphasis on designing flexible manufacturing systems, particularly in the electronics manufacturing industry, is bringing automation and robotics technologies to the factory at increasing rates. The rate of advance in these technologies raises serious concerns among engineers and managers about how to proceed in building modern manufacturing systems. A large portion of this uncertainty results from the difficulty of fitting technological advances into the existing models of manufacturing. What is needed is a new framework within which to perceive automation and robotics which will permit the adoption of more encompassing design strategies and principles to be followed in the practice of modernizing and maintaining advanced electronics manufacturing systems. This paper provides a framework that might be adopted to structure new strategies for incorporating automation and robotics in manufacturing. The approach is one that we at SRI have found useful in considering automation and robotics issues for the NASA Space Station and other complex systems which need to incorporate new technologies throughout long lifetimes. These same issues are becoming increasingly important in electronics manufacturing system design and development.     

Extinction of dinosaurs: a possible novel cause.

A novel cause of mass extinction of fauna close to the (K/T) Boundary is suggested. A large amount of non-protein amino acids (AIB and ISOVAL) has been observed close to this event. It is speculated that these amino acids may be toxic and are responsible for the extinction. The toxicity level is estimated for this suggestion to be true and experimentalists are encouraged to test this level of toxicity for the amino acids.     

The Freja F3C Cold Plasma Analyzer

The F3C Cold Plasma Analyzer (CPA) instrument on theFreja spacecraft is designed to measure the energy per unit charge (E/Q) of ions oe electrons in the range 0<E/Q<200 V and complements the observations made by the F3H Hot Plasma Experiment. The CPA sensor, which is deployed on a boom, is an electrostatic analyzer which produces angle/energy images of particles incident on the sensor in a plane perpendicular to the boom axis. Charged particles incident normal to the CPA sensor housing axis of symmetry, which coincides with the boom axis, pass through collimators and enter a semi-spherical electrostatic analyzer which disperses particles in energy and azimuthal angle of arrival onto an imaging MCP detector thus producing images of the particle distributions in a plane perpendicular to the boom axis. Measurements are transmitted either as discrete 16×16 (angle/energy) images or as parameters related to the incident particle distribution function. Pixels in the discrete images are separated approximately equally in azimuthal angle while the 16 energy bins are separated approximately geometrically in energy. The ratio of the maximum to minimum energy imaged is programmable up to a maximum of more than a factor of ten, and the energy range itself is also under the control of the processor and can be varied by more than an order of magnitude. The density dynamic range of the sensor is increased by the introduction of an electrostatic gating system between the entrance aperture and the analyzer which can be used to duty-cycle low-energy electrons into the sensor thus keeping the count rate within appropriate levels. To reduce the effects of spacecraft induced perturbations on the lower-energy particle distributions, the sensor portion of the instrument is deployed on a 2 m long boom, perpendicular to the spacecraft spin axis. Spacecraft rotation is used to recover complete (4) angle/energy distributions every half spin period. In addition, the sensor skin may be biased with respect to the spacecraft ground to offset effects due to spacecraft charging. Current to the skin is monitored, making the exterior of the sensor equivalent to a large cylindrical Langmuir probe. Two separate processing paths for signals from the MCP anode may be chosen; slow and rast. The slow pulse processing path provides discrete angle/energy images at a nominal rate of 10 images per second and a peak burst mode rate of 100 images per second. The fast analog or current mode path provides crude parameterized estimates of densities, temperatures and drift velocities at nominal rates of up to 1000 parameters per second with a burst rate near 6000 parameters per second. Observations of cold ions and electrons in an unperturbed ionospheric plasma are presented which demonstrate the functionality of the instrument. Suprathermal ion observations in a transverse ion energization or acceleration region are also shown which demonstrate many of the small-scale features of these events.The Canadian Government's right to retain a non-exclusive, royalty free licence in and to any copyright is acknowledge.