Charged-particle mutagenesis II. Mutagenic effects of high energy charged particles in normal human fibroblasts.

The biological effects of high LET charged particles are a subject of great concern with regard to the prediction of radiation risk in space. In this report, mutagenic effects of high LET charged particles are quantitatively measured using primary cultures of human skin fibroblasts, and the spectrum of induced mutations are analyzed. The LET of the charged particles ranged from 25 KeV/micrometer to 975 KeV/micrometer with particle energy (on the cells) between 94-603 MeV/u. The X-chromosome linked hypoxanthine guanine phosphoribosyl transferase (hprt) locus was used as the target gene. Exposure to these high LET charged particles resulted in exponential survival curves; whereas, mutation induction was fitted by a linear model. The Relative Biological Effect (RBE) for cell-killing ranged from 3.73 to 1.25, while that for mutant induction ranged from 5.74 to 0.48. Maximum RBE values were obtained at the LET of 150 keV/micrometer. The inactivation cross-section (alpha i) and the action cross-section for mutant induction (alpha m) ranged from 2.2 to 92.0 micrometer2 and 0.09 to 5.56 x 10(-3) micrometer2, respectively. The maximum values were obtained by 56Fe with an LET of 200 keV/micrometer. The mutagenicity (alpha m/alpha i) ranged from 2.05 to 7.99 x 10(-5) with the maximum value at 150 keV/micrometer. Furthermore, molecular analysis of mutants induced by charged particles indicates that higher LET beams are more likely to cause larger deletions in the hprt locus.     

Oxygen line mapping of SN 1006 with Suzaku

SN 1006 is one of the supernova remnants (SNRs) with relatively low-temperature electrons, considering the young age of just 1000 years. We carried out SN 1006 mapping observations with the X-ray Imaging Spectrometers (XIS) and the Hard X-ray Detector (HXD) onboard Suzaku, the fifth Japanese X-ray satellite. Thanks to the excellent spectral resolution of XIS in the soft X-ray band, H-like and He-like oxygen emission lines were clearly detected, and we could make a map of the line intensity, and as well as a flux and the photon index of nonthermal component. We found that these parameters have spatial dependences from region to region in the SNR; the north region is bright in nonthermal, while dim in thermal; the east region is bright in both nonthermal and thermal; the inner region shows dim nonthermal and bright thermal emission. The photon index is the smallest in the north region.