Simulation experiments of the effect of space environment on bacteriophage and DNA thin films.

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy. To provide experimental evidence for the hypothesis that interplanetary transfer of the genetic material is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (lambda=254 nm) and high vacuum (10(-4) Pa). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.     

A Geosynchronous Orbit Search Strategy

Since more than 10 years there is evidence that small-size space debris is accumulating in the geosynchronous orbit (GEO), probably as the result of breakups. Two break-ups have been reported in GEO. The 1978 break-up of an EKRAN 2 satellite, SSN 10365, was identified in 1992, and in 1992 a Titan 3C Transtage, SSN 3432, break-up produced at least twenty observable pieces. Subsequently several nations performed optical surveys of the GEO region in the form of independent observation campaigns. Such surveys suffer from the fact that the field of view of optical telescopes is small compared with the total area covered by the GEO ring. As a consequence only a small volume of the orbital element-magnitude-space is covered by each individual survey. Results from these surveys are thus affected by observational biases and therefore difficult to compare. This paper describes the development of a common search strategy to overcome these limitations. The strategy optimizes the sampling for objects in orbits similar to the orbits of the known GEO population but does not exclude the detection of objects with other orbital planes. A properly designed common search strategy clearly eases the comparison of results from different groups and the extrapolation from the sparse (biased) samples to the entire GEO environment.     

The Brazilian scientific microsatellite SACI-1

The National Space Research Institute (INPE) is developing the first Brazilian Scientific Microsatellite (SACI-1) based on the vanguard technology and on the experience acquired through projects developed by Brazilian Space Program. The SACI-1 is a 750km polar orbit satellite. The spacecraft will combine spin stabilization with geomagnetic control and has a total mass of 60 kg. The overall dimensions are 640×470×470 mm. The SACI-1 satellite shall be launched together with CBERS (China-Brazil Earth Resource Satellite). Its platform is being designed for multiple mission applications. The Brazilian Academy of Sciences has selected four scientific payloads that characterize the mission. The scientific experiments are: ORCAS (Solar and Anomalous Cosmic Rays Observation in the Magnetosphere), PLASMEX (Study of Plasma Bubbles), FOTSAT (Airglow Photometer), and MAGNEX (Geomagnetic Experiment).     

Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

The Space within Fisherman's Folly: Playing with a Puzzle in Mereotopology

Abstract

In this paper we propose a spatial ontology for reasoning about holes, rigid objects and a string, taking a classical puzzle as a motivating example. In this ontology the domain is composed of spatial regions whereby a theory about holes is defined over a mereotopological basis. Within this theory we define a data structure, named chain, that facilitates a clear and efficient representation of the puzzle states and its solution.     

Longitudinal variation of the equatorial ionosphere: Modeling and experimental results

We describe a new version of the Parameterized Regional Ionospheric Model (PARIM) which has been modified to include the longitudinal dependences. This model has been reconstructed using multidimensional Fourier series. To validate PARIM results, the South America maps of critical frequencies for the E (foE) and F (foF2) regions were compared with the values calculated by Sheffield Plasmasphere-Ionosphere Model (SUPIM) and IRI representations. PARIM presents very good results, the general characteristics of both regions, mainly the presence of the equatorial ionization anomaly, were well reproduced for equinoctial conditions of solar minimum and maximum. The values of foF2 and hmF2 recorded over Jicamarca (12°S; 77°W; dip lat. 1°N; mag. declination 0.3°) and sites of the conjugate point equatorial experiment (COPEX) campaign Boa Vista (2.8°N; 60.7°W; dip lat. 11.4°; mag. declination −13.1°), Cachimbo (9.5°S; 54.8°W; dip lat. −1.8°; mag. declination −15.5°), and Campo Grande (20.4°S; 54.6°W; dip lat. −11.1°; mag. declination −14.0°) have been used in this work. foF2 calculated by PARIM show good agreement with the observations, except during morning over Boa Vista and midnight-morning over Campo Grande. Some discrepancies were also found for the F-region peak height (hmF2) near the geomagnetic equator during times of F₃ layer occurrences. IRI has underestimated both foF2 and hmF2 over equatorial and low latitude sectors during evening-nighttimes, except for Jicamarca where foF2 values were overestimated.     

Cause and Effect At the Magnetopause

Recent analyses of spacecraft data, especially AMPTE/IRM data, provide a test of reconnection theory; an analysis for the signature of a local tangential stress balance in a one-dimensional time-stationary rotational discontinuity has left crucial questions unanswered. A key result is that the electron temperature profile inward through the magnetopause current sheet shows heating followed by cooling. Electrons must be one of the carriers of the current; hence this result reflects the sign of E · J in the frame of reference of the magnetopause current carriers. Since the current is directed from dawn to dusk, the inescapable conclusion is that the electric field must reverse within the current sheet. This is direct evidence of a load–dynamo combination; in that dynamo, energy is transferred from the solar wind plasma to the electromagnetic field. A dynamo is not included in the reconnection model which includes only the electrical load; therefore, we argue that the reconnection problem is improperly posed. A second compelling observation is a remarkable difference of the normal component of the plasma velocity between inbound and outbound crossings. For an inbound crossing (outward current meander) this component does reverse, but not quite as assumed in the reconnection model; on the other hand, for outbound crossings of the spacecraft (corresponding to erosion) there is no reversal at all. The normal component is approximately constant at 20 km s^-1, anti-Sunward throughout. Since the typical motion of the magnetopause is 10 km s^-1 this revealing result shows that solar wind plasma can go across the magnetopause, even onto closed field lines to feed the low latitude boundary layer. This is in stark contrast to the reconnection model where the plasma goes to open field lines. The interaction can be understood by appealing to Poynting's theorem, where E · J describes the net effect on or by the plasma. Time-dependent terms (even in the initial conditions) must be used so that it is possible to draw upon energy which has been stored locally in both electrical and magnetic forms. An extended discussion of observational results from ground-based, rocket, and satellite instruments indicate the impulsive nature of the solar wind–magnetospheric interaction. There is a lot of plasma involved in this interaction, over 10²⁷ ions electrons^-1 per second; the anti-Sunward flow takes place in the low latitude boundary layer. There is no flux catastrophe produced by this flow since the frozen-field theorem does not hold for plasma transfer across the magnetopause. The LLBL completely envelops the plasma sheet; the LLBL is the source of its plasma, not the plasma mantle as hypothesized in the reconnection model of the magnetotail. A number of serious errors have occurred in some articles in the literature on reconnection, and we list and discuss the most important of these. In the conclusion it is emphasized that the failure to provide a viable energy source, within the necessary spatial and temporal constraints, is responsible for the failure of reconnection model. This does not mean that the state of interconnection between the geomagnetic field and the interplanetary magnetic field can not change, but it does mean that the advocated process is not relevant to such changes. True reconnection requires that the electric field has a curl so that an electromotive force = E · dl = -d_Mdt exists through which energy can be interchanged with stored magnetic energy.     

Local-time variations of geomagnetic disturbances during intense geomagnetic storms and possible association with their interplanetary causes

In the present paper the local-time variations in the disturbance of the geomagnetic-field horizontal component (H) for eight intense geomagnetic storms that occurred during the descending phase of solar cycle 23 have been analyzed. The study was based on the plot of contour lines of the H-depletion intensity in the plane local time versus universal time (LT–UT maps) with the objective of observing how the morphology and evolution of the ring current is mapped into the surface of the Earth in presence of intense geomagnetic storms.