Changes in vacuolation in the root apex cells of soybean seedlings in microgravity.

Changes in the vacuolation in root apex cells of soybean (Glycine max L. [Merr.]) seedlings grown in microgravity were investigated. Spaceflight and ground control seedlings were grown in the absence or presence of KMnO4 (to remove ethylene) for 6 days. After landing, in order to study of cell ultrastructure and subcellular free calcium ion distribution, seedling root apices were fixed in 2.5% (w/v) glutaraldehyde in 0.1 M cacodylate buffer and 2% (w/v) glutaraldehyde, 2.5% (w/v) formaldehyde, 2% (w/v) potassium antimonate K[Sb(OH)6] in 0.1 M K2HPO4 buffer with an osmolarity (calculated theoretically) of 0.45 and 1.26 osmol. The concentrations of ethylene in all spaceflight canisters were significantly higher than in the ground control canisters. Seedling growth was reduced in the spaceflight-exposed plants. Additionally, the spaceflight-exposed plants exhibited progressive vacuolation in the root apex cells, particularly in the columella cells, to a greater degree than the ground controls. Plasmolysis was observed in columella cells of spaceflight roots fixed in solutions with relatively high osmolarity (1.26 osmol). The appearance of plasmolysis permitted the evaluation of the water status of cells. The water potential of the spaceflight cells was higher than the surrounding fixative solution. A decrease in osmotic potential and/or an increase in turgor potential may have induced increases in cell water potential. However, the plasmolysed (i.e. non-turgid) cells implied that increases in water potential were accompanied with a decrease in osmotic potential. In such cells changes in vacuolation may have been involved to maintain turgor pressure or may have been a result of intensification of other vacuolar functions like digestion and storage.     

Modelling of the electromagnetic field in the interplanetary space and in the Earth's magnetosphere

A magnetohydrodynamic model of the solar wind flow is constructed using a kinematic approach. It is shown that a phenomenological conductivity of the solar wind plasma plays a key role in the forming of the interplanetary magnetic field (IMF) component normal to the ecliptic plane. This component is mostly important for the magnetospheric dynamics which is controlled by the solar wind electric field. A simple analytical solution for the problem of the solar wind flow past the magnetosphere is presented. In this approach the magnetopause and the Earth's bow shock are approximated by the paraboloids of revolution. Superposition of the effects of the bulk solar wind plasma motion and the magnetic field diffusion results in an incomplete screening of the IMF by the magnetopause. It is shown that the normal to the magnetopause component of the solar wind magnetic field and the tangential component of the electric field penetrated into the magnetosphere are determined by the quarter square of the magnetic Reynolds number. In final, a dynamic model of the magnetospheric magnetic field is constructed. This model can describe the magnetosphere in the course of the severe magnetic storm. The conditions under which the magnetospheric magnetic flux structure is unstable and can drive the magnetospheric substorm are discussed. The model calculations are compared with the observational data for September 24–26, 1998 magnetic storm (Dst _min=−205 nT) and substorm occurred at 02:30 UT on January 10, 1997. This revised version was published online in August 2006 with corrections to the Cover Date.     

An Overview of Jets and Outflows in Stellar Mass Black Holes

In this article, we will briefly review the current empirical understanding of the relation between accretion state and outflows in accreting stellar mass black holes. The focus will be on the empirical connections between X-ray states and relativistic (‘radio’) jets, although we are now also able to draw accretion disc winds into the picture in a systematic way. We will furthermore consider the latest attempts to measure/order jet power, and to compare it to other (potentially) measurable quantities, most importantly black hole spin.     

Constellation analysis of an integrated AIS/remote sensing spaceborne system for ship detection

A future system integrating data from remote sensing and upcoming AIS satellites is analyzed through the development of a novel design method for global, discontinuous coverage constellations. It is shown that 8 AIS satellites suffice to guarantee global coverage and a ship location update of 50 min if the spaceborne AIS receiver has a swath of 2800 nm. Furthermore, synergic utilization of COSMO/SkyMed and Radarsat-C data would provide a mean revisit time of 7 h, with AIS information available within 25 min from SAR data acquisition.     

Numerical modeling of inhomogeneous orographic wave influence on planetary waves in the middle atmosphere

Parameterization of dynamical and thermal effects of stationary orographic gravity waves (OGWs) generated by the Earth’s surface topography is incorporated into a numerical model of general circulation of the middle and upper atmosphere. Responses of atmospheric general circulation and characteristics of planetary waves at altitudes from the troposphere up to the thermosphere to the effects of OGWs propagating from the earth surface are studied. Changes in atmospheric circulation and amplitudes of planetary waves due to variations of OGW generation and propagation in different seasons are considered. It is shown that during solstices the main OGW dynamical and heat effects occur in the middle atmosphere of winter hemispheres, where changes in planetary wave amplitudes due to OGWs may reach up to 50%. During equinoxes OGW effects are distributed more homogeneously between northern and southern hemispheres.     

Simulating planetary wave propagation to the upper atmosphere during stratospheric warming events at different mountain wave scenarios

Parameterization schemes of atmospheric normal modes (NMs) and orographic gravity waves (OGWs) have been implemented into the mechanistic Middle and Upper Atmosphere Model (MUAM) simulating atmospheric general circulation. Based on the 12-members ensemble of runs with the MUAM, a composite of the stratospheric warming (SW) has been constructed using the UK Met Office data as the lower boundary conditions. The simulation results show that OGW amplitudes increase at altitudes above 30 km in the Northern Hemisphere after the SW event. At altitudes of about 50 km, OGWs have largest amplitudes over North American and European mountain systems before and during the composite SW, and over Himalayas after the SW. Simulations demonstrate substantial (up to 50–70%) variations of amplitudes of stationary planetary waves (PWs) during and after the SW in the mesosphere-lower thermosphere of the Northern Hemisphere. Westward travelling NMs have amplitude maxima not only in the Northern, but also in the Southern Hemisphere, where these modes have waveguides in the middle and upper atmosphere. Simulated variations of PW and NM amplitudes correspond to changes in the mean zonal wind, EP-fluxes and wave refractive index at different phases of the composite SW events. Inclusion of the parameterization of OGW effects leads to decreases in amplitudes (up to 15%) of almost all SPWs before and after the SW event and their increase (up to 40–60%) after the SW in the stratosphere and mesosphere at middle and high northern latitudes. It is suggested that observed changes in NM amplitudes in the Southern Hemisphere during SW could be caused by divergence of increased southward EP-flux. This EP-flux increases due to OGW drag before SW and extends into the Southern Hemisphere.     

Microorganisms and enzyme activity in permafrost after removal of long-term cold stress

Associations of immobilized microbial cells and organic-mineral complexes containing active enzymes are resistant to long-term (from tens of thousands to millions of years) effects of extremely low temperatures. This association enables the cells to restore their metabolic activity during permafrost thawing, because interactions with the heterogenous medium is made possible by the availability of active immobilized enzymes. The long-term effect of the cold probably favors an adaptational change of microbial metabolism that activates enzymes and cells during thawing.     

Decentralized formation pose estimation for spacecraft swarms

For spacecraft swarms, the multi-agent localization algorithm must scale well with the number of spacecraft and adapt to time-varying communication and relative sensing networks. In this paper, we present a decentralized, scalable algorithm for swarm localization, called the Decentralized Pose Estimation (DPE) algorithm. The DPE considers both communication and relative sensing graphs and defines an observable local formation. Each spacecraft jointly localizes its local subset of spacecraft using direct and communicated measurements. Since the algorithm is local, the algorithm complexity does not grow with the number of spacecraft in the swarm. As part of the DPE, we present the Swarm Reference Frame Estimation (SRFE) algorithm, a distributed consensus algorithm to co-estimate a common Local-Vertical, Local-Horizontal (LVLH) frame. The DPE combined with the SRFE provides a scalable, fully-decentralized navigation solution that can be used for swarm control and motion planning. Numerical simulations and experiments using Caltech’s robotic spacecraft simulators are presented to validate the effectiveness and scalability of the DPE algorithm.     

Toward an Understanding of the Systematic Uncertainties in Deriving the Primordial Helium Abundance from H II Region Observations

Recent determinations of the primordial He abundance have given significantly different results. We are attempting to identify some of the causes of these differences and propose observational solutions. Here we identify a systematic difference in how the data are interpreted (differences in corrections for the presence of neutral helium) and the importance of a systematic bias towards lower derived helium abundances (underestimating the presence of underlying stellar absorption).