Cave biosignature suites: microbes,minerals, and Mars

Earth's subsurface offers one of the best possible sites to search for microbial life and the characteristic lithologies that life leaves behind. The subterrain may be equally valuable for astrobiology. Where surface conditions are particularly hostile, like on Mars, the subsurface may offer the only habitat for extant lifeforms and access to recognizable biosignatures. We have identified numerous unequivocally biogenic macroscopic, microscopic, and chemical/geochemical cave biosignatures. However, to be especially useful for astrobiology, we are looking for suites of characteristics. Ideally, "biosignature suites" should be both macroscopically and microscopically detectable, independently verifiable by nonmorphological means, and as independent as possible of specific details of life chemistries--demanding (and sometimes conflicting) criteria. Working in fragile, legally protected environments, we developed noninvasive and minimal impact techniques for life and biosignature detection/characterization analogous to Planetary Protection Protocols. Our difficult field conditions have shared limitations common to extraterrestrial robotic and human missions. Thus, the cave/subsurface astrobiology model addresses the most important goals from both scientific and operational points of view. We present details of cave biosignature suites involving manganese and iron oxides, calcite, and sulfur minerals. Suites include morphological fossils, mineral-coated filaments, living microbial mats and preserved biofabrics, 13C and 34S values consistent with microbial metabolism, genetic data, unusual elemental abundances and ratios, and crystallographic mineral forms.     

Psychosocial effects of adjustment in Antarctica: lessons for long-duration spaceflight

This paper examines the utility of remote, isolated Antarctic research stations as analogs for long-duration spaceflights from the perspective of psychosocial processes of adaptation and adjustment. Certain features of the physical and man-made environments found in Antarctica are similar to those that will be encountered in outer space. In both settings, men and women are likely to experience a number of physiological and psychological changes in response to the extreme environmental conditions and the prolonged isolation and confinement. Biomedical research in Antarctica provides an opportunity to study the causes of these changes and to develop strategies for reducing the risks to health and well-being before they pose a serious threat to crew safety and mission success. A number of lessons for long-duration spaceflight are examined, including screening and selection of personnel; training programs designed to facilitate individual adjustment and group adaptation and minimize group conflict; identification of optimal leadership characteristics for small, isolated groups; an understanding of social dynamics and group "microcultures" necessary for the organization and management of small but heterogeneous groups; organization of work activities; facility design; and support infrastructure.     

使用数字电视通道的多媒体信息广播

本文概述了数字电视通道内广播多媒体信息的一种新的系统结构。所提出的方案是建立在ＤＳＭ－ＣＣ（数字存贮媒体指令和控制）功能的基础上，并使用了一种最近拟定的，能有效传送多媒体信息的协议。上述的信息与在ＷＷＷ环境中使用的ＨＴＭＬ文件的特性相同，因此，它可以包含本文、图像声音，和按照超本文编制的动画。     

Ground based ISS payload microgravity disturbance assessments

In order to verify that the International Space Station (ISS) payload facility racks do not disturb the microgravity environment of neighboring facility racks and that the facility science operations are not compromised, a testing and analytical verification process must be followed. Currently no facility racks have taken this process from start to finish. The authors are participants in implementing this process for the NASA Glenn Research Center (GRC) Fluids and Combustion Facility (FCF). To address the testing part of the verification process, the Microgravity Emissions Laboratory (MEL) was developed at GRC. The MEL is a 6 degree of freedom inertial measurement system capable of characterizing inertial response forces (emissions) of components, sub-rack payloads, or rack-level payloads down to 10(-7) g's. The inertial force output data, generated from the steady state or transient operations of the test articles, are utilized in analytical simulations to predict the on-orbit vibratory environment at specific science or rack interface locations. Once the facility payload rack and disturbers are properly modeled an assessment can be made as to whether required microgravity levels are achieved. The modeling is utilized to develop microgravity predictions which lead to the development of microgravity sensitive ISS experiment operations once on-orbit. The on-orbit measurements will be verified by use of the NASA GRC Space Acceleration Measurement System (SAMS). The major topics to be addressed in this paper are: (1) Microgravity Requirements, (2) Microgravity Disturbers, (3) MEL Testing, (4) Disturbance Control, (5) Microgravity Control Process, and (6) On-Orbit Predictions and Verification.     

Setting a standard: the limulus amebocyte lysate assay and the assessment of microbial contamination on spacecraft surfaces

Historically, colony-forming units as determined by plate cultures have been the standard unit for microbiological analysis of environmental samples, medical diagnostics, and products for human use. However, the time and materials required make plate cultures expensive and potentially hazardous in the closed environments of future NASA missions aboard the International Space Station and missions to other Solar System targets. The Limulus Amebocyte Lysate (LAL) assay is an established method for ensuring the sterility and cleanliness of samples in the meat-packing and pharmaceutical industries. Each of these industries has verified numerical requirements for the correct interpretation of results from this assay. The LAL assay is a rapid, point-of-use, verified assay that has already been approved by NASA Planetary Protection as an alternate, molecular method for the examination of outbound spacecraft. We hypothesize that standards for molecular techniques, similar to those used by the pharmaceutical and meat-packing industries, need to be set by space agencies to ensure accurate data interpretation and subsequent decision making. In support of this idea, we present research that has been conducted to relate the LAL assay to plate cultures, and we recommend values obtained from these investigations that could assist in interpretation and analysis of data obtained from the LAL assay.     

Adaptation of neuromuscular activation patterns during treadmill walking after long-duration space flight

The precise neuromuscular control needed for optimal locomotion, particularly around heel strike and toe off, is known to he compromised after short duration (8- to 15-day) space flight. We hypothesized here that longer exposure to weightlessness would result in maladaptive neuromuscular activation during postflight treadmill walking. We also hypothesized that space flight would affect the ability of the sensory-motor control system to generate adaptive neuromuscular activation patterns in response to changes in visual target distance during postflight treadmill walking. Seven crewmembers, who completed 3- to 6-month missions, walked on a motorized treadmill while visually fixating on a target placed 30 cm (NEAR) or 2 m (FAR) from the subject's eyes. Electronic foot switch data and surface electromyography were collected from selected muscles of the right lower limb. Results indicate that the phasic features of neuromuscular activation were moderately affected and the relative amplitude of activity in the tibialis anterior and rectus femoris around toe off changed after space flight. Changes also were evident after space flight in how these muscles adapted to the shift in visual target distance.