Long-term manipulations of intact microbial mat communities in a greenhouse collaboratory: simulating earth's present and past field environments

Photosynthetic microbial mat communities were obtained from marine hypersaline saltern ponds, maintained in a greenhouse facility, and examined for the effects of salinity variations. Because these microbial mats are considered to be useful analogs of ancient marine communities, they offer insights about evolutionary events during the >3 billion year time interval wherein mats co-evolved with Earth's lithosphere and atmosphere. Although photosynthetic mats can be highly dynamic and exhibit extremely high activity, the mats in the present study have been maintained for >1 year with relatively minor changes. The major groups of microorganisms, as assayed using microscopic, genetic, and biomarker methodologies, are essentially the same as those in the original field samples. Field and greenhouse mats were similar with respect to rates of exchange of oxygen and dissolved inorganic carbon across the mat-water interface, both during the day and at night. Field and greenhouse mats exhibited similar rates of efflux of methane and hydrogen. Manipulations of salinity in the water overlying the mats produced changes in the community that strongly resemble those observed in the field. A collaboratory testbed and an array of automated features are being developed to support remote scientific experimentation with the assistance of intelligent software agents. This facility will permit teams of investigators the opportunity to explore ancient environmental conditions that are rare or absent today but that might have influenced the early evolution of these photosynthetic ecosystems.     

Extragalactic Abundances of Hydrogen,Deuterium and Helium New Steps,Missteps and Next Steps

Estimates of the deuterium abundance in quasar absorbers are reviewed, including a brief account of incorrect claims published by the author and a brief review of the problem of hydrogen contamination. It is concluded that the primordial abundance may be universal with a value (D/H)P 10-4, within about a factor of two, corresponding to _Bh _0.7 ² 0.0fs2 or 10 2.7 in the Standard Big Bang. This agrees with current limits on primordial helium, YP 0.243, which are shown to be surprisingly insensitive to models of stellar enrichment. It also agrees with a tabulated sum of the total density of baryons in observed components. Much lower primordial deuterium ( 2 × 10^-5) is also possible but disagrees with currently estimated helium abundances; the larger baryon density in this case fits better with current models of the Lyman- forest but requires the bulk of the baryons to be in some currently uncounted form.