High-Definition Television System Onboard Lunar Explorer Kaguya (SELENE) and Imaging of the Moon and the Earth

The High-Definition television (HDTV) system onboard the Japanese lunar explorer Kaguya (SELENE) consists of a telephotographic camera and a wide-angle camera that each have 2.2 M-pixel IT-CCDs (interline transfer charge-coupled devices) and LSIs (large-scale integrated circuits) of the several-million-gates class. One minute-long motion pictures acquired by the HDTV system at 30 fps (frames per second) are recorded in a 1 GB semiconductor memory after compression, and then transmitted to a ground station. In the development of the space-going HDTV system, a commercial ground-model HDTV system was extensively modified and evaluated for its suitability to withstand the harsh environment of space through environmental tests. The HDTV acquired a total of 6.3 TB of movies and still images of the Earth and the Moon over the mission period that started on September 29, 2007, and ended on June 11, 2009. Footage of an “Earth-rise” and an “Earth-set” on the lunar horizon were captured for the first time by the HDTV system. During a lunar eclipse, images of the Earth’s “diamond ring” were acquired for the first time. The CCDs and the instruments used in the system remained in good working order throughout the mission period, despite the harsh space environment, which suggests a potential new approach to the development of instruments for use in space.     

Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

The 53 kDa tumor suppressor protein p53 is generally thought to contribute to the genetic stability of cells and to protect cells from DNA damage through the activity of p53-centered signal transduction pathways. To clarify the effect of space radiation on the expression of p53-dependent regulated genes, gene expression profiles were compared between two human cultured lymphoblastoid cell lines: one line (TSCE5) has a wild-type p53 gene status, and the other line (WTK1) has a mutated p53 gene status. Frozen human lymphoblastoid cells were stored in a freezer in the International Space Station (ISS) for 133 days. Gene expression was analyzed using DNA chips after culturing the space samples for 6 h on the ground after their return from space. Ground control samples were also cultured for 6 h after being stored in a frozen state on the ground for the same time period that the frozen cells were in space. p53-Dependent gene expression was calculated from the ratio of the gene expression values in wild-type p53 cells and in mutated p53 cells. The expression of 50 p53-dependent genes was up-regulated, and the expression of 94 p53-dependent genes was down-regulated after spaceflight. These expression data identified genes which could be useful in advancing studies in basic space radiation biology. The biological meaning of these results is discussed from the aspect of gene functions in the up- and down-regulated genes after exposure to low doses of space radiation.     

Preliminary results of space experiment: Implications for the effects of space radiation and microgravity on survival and mutation induction in human cells

In view of the concern for the health of astronauts that may one day journey to Mars or the Moon, we investigated the effect that space radiation and microgravity might have on DNA damage and repair. We sent frozen human lymphoblastoid TK6 cells to the International Space Station where they were maintained under frozen conditions during a 134-day mission (14 November 2008 to 28 March 2009) except for an incubation period of 8 days under 1G or μG conditions in a CO₂ incubator. The incubation period started after 100 days during which the cells had been exposed to 54 mSv of space radiation. The incubated cells were then refrozen, returned to Earth, and compared to ground control samples for the determination of the influence of microgravity on cell survival and mutation induction. The results for both varied from experiment to experiment, yielding a large SD, but the μG sample results differed significantly from the 1G sample results for each of 2 experiments, with the mean ratio of μG to 1G being 0.55 for the concentration of viable cells and 0.59 for the fraction of thymidine kinase deficient (TK⁻) mutants. Among the mutants, non-loss of zygosity events (point mutations) were less frequent (31%) after μG incubation than after 1G incubation, which might be explained by the influence of μG on cellular metabolic or physiological function. Additional experiments are needed to clarify the effect of μG interferes on DNA repair.