Carotenoid analysis of halophilic archaea by resonance Raman spectroscopy

Recently, halite and sulfate evaporate rocks have been discovered on Mars by the NASA rovers, Spirit and Opportunity. It is reasonable to propose that halophilic microorganisms could have potentially flourished in these settings. If so, biomolecules found in microorganisms adapted to high salinity and basic pH environments on Earth may be reliable biomarkers for detecting life on Mars. Therefore, we investigated the potential of Resonance Raman (RR) spectroscopy to detect biomarkers derived from microorganisms adapted to hypersaline environments. RR spectra were acquired using 488.0 and 514.5 nm excitation from a variety of halophilic archaea, including Halobacterium salinarum NRC-1, Halococcus morrhuae, and Natrinema pallidum. It was clearly demonstrated that RR spectra enhance the chromophore carotenoid molecules in the cell membrane with respect to the various protein and lipid cellular components. RR spectra acquired from all halophilic archaea investigated contained major features at approximately 1000, 1152, and 1505 cm(-1). The bands at 1505 cm(-1) and 1152 cm(-1) are due to in-phase C=C (nu(1) ) and C-C stretching ( nu(2) ) vibrations of the polyene chain in carotenoids. Additionally, in-plane rocking modes of CH(3) groups attached to the polyene chain coupled with C-C bonds occur in the 1000 cm(-1) region. We also investigated the RR spectral differences between bacterioruberin and bacteriorhodopsin as another potential biomarker for hypersaline environments. By comparison, the RR spectrum acquired from bacteriorhodopsin is much more complex and contains modes that can be divided into four groups: the C=C stretches (1600-1500 cm(-1)), the CCH in-plane rocks (1400-1250 cm(-1)), the C-C stretches (1250-1100 cm(-1)), and the hydrogen out-of-plane wags (1000-700 cm(-1)). RR spectroscopy was shown to be a useful tool for the analysis and remote in situ detection of carotenoids from halophilic archaea without the need for large sample sizes and complicated extractions, which are required by analytical techniques such as high performance liquid chromatography and mass spectrometry.     

Interplanetary Origin of Intense,Superintense and Extreme Geomagnetic Storms

We present a review on the interplanetary causes of intense geomagnetic storms (Dst≤−100 nT), that occurred during solar cycle 23 (1997–2005). It was reported that the most common interplanetary structures leading to the development of intense storms were: magnetic clouds, sheath fields, sheath fields followed by a magnetic cloud and corotating interaction regions at the leading fronts of high speed streams. However, the relative importance of each of those driving structures has been shown to vary with the solar cycle phase. Superintense storms (Dst≤−250 nT) have been also studied in more detail for solar cycle 23, confirming initial studies done about their main interplanetary causes. The storms are associated with magnetic clouds and sheath fields following interplanetary shocks, although they frequently involve consecutive and complex ICME structures. Concerning extreme storms (Dst≤−400 nT), due to the poor statistics of their occurrence during the space era, only some indications about their main interplanetary causes are known. For the most extreme events, we review the Carrington event and also discuss the distribution of historical and space era extreme events in the context of the sunspot and Gleissberg solar activity cycles, highlighting a discussion about the eventual occurrence of more Carrington-type storms.     

Optical Ranging System Employing a High Power Injection Laser Diode

For many military applications small, lightweight range finders are required that operate at a high pulse repetition rate. It will be shown that a ranging system with an injection laser in the transmitter can fulfill these requirements. The performance of an optical ranging system employing a pulsed gallium arsenide laser diode transmitter, with peak powers up to 100 watts at room temperature is described. After a brief review of fundamental system parameters, calculations of the range capability of the system are given, based upon the Poisson distribution of photoelectron statistics. A discussion is presented on the experimental support of theoretical analysis.     

On uncertainties in carbon flux modelling and remotely sensed data assimilation: The Brasschaat pixel case

Uncertainty on carbon fluxes is determined by the uncertainties of ecosystem model structure, data and model parameter uncertainties and the temporal and spatial inaccuracy of the input data retrieval. The objective of this paper is to understand the error propagation and uncertainty of evaporative fraction (EF), soil moisture content (SMC) and water limited net ecosystem productivity (NEP). In this respect, C-Fix and spaceborne remote sensing are used for the ‘Brasschaat’ pixel. A simple model based on error theory and a Monte-Carlo approach are used. Different error scenarios are implemented to assess input uncertainty on EF, SMC and NEP as estimated with C-Fix.     

Adaptive recognition and correction of baseline shifts from collocated GPS and accelerometer using two phases Kalman filter

The real-time recognition and precise correction of baseline shifts in strong-motion records is a critical issue for GPS and accelerometer combined processing. This paper proposes a method to adaptively recognize and correct baseline shifts in strong-motion records by utilizing GPS measurements using two phases Kalman filter. By defining four kinds of learning statistics and criteria, the time series of estimated baseline shifts can be divided into four time intervals: initialization, static, transient and permanent. During the time interval in which the transient baseline shift is recognized, the dynamic noise of the Kalman filter system and the length of the baseline shifts estimation window are adaptively adjusted to yield a robust integration solution. The validations from an experimental and real datasets show that acceleration baseline shifts can be precisely recognized and corrected, thus, the combined system adaptively adjusted the estimation strategy to get a more robust solution.     

The Galactic Cosmic Ray Intensity over the Past 106–109 Years as Recorded by Cosmogenic Nuclides in Meteorites and Terrestrial Samples

Concentrations of stable and radioactive nuclides produced by cosmic ray particles in meteorites allow us to track the long term average of the primary flux of galactic cosmic rays (GCR). During the past ～10 Ma, the average GCR flux remained constant over timescales of hundreds of thousands to millions of years, and, if corrected for known variations in solar modulation, also during the past several years to hundreds of years. Because the cosmic ray concentrations in meteorites represent integral signals, it is difficult to assess the limits of uncertainty of this statement, but they are larger than the often quoted analytical and model uncertainties of some 30%. Time series of concentrations of the radionuclide ¹⁰Be in terrestrial samples strengthen the conclusions drawn from meteorite studies, indicating that the GCR intensity on a ～0.5 million year scale has remained constant within some ±10% during the past ～10 million years. The very long-lived radioactive nuclide ⁴⁰K allows to assess the GCR flux over about the past one billion years. The flux over the past few million years has been the same as the longer-term average in the past 0.5–1 billion years within a factor of ～1.5. However, newer data do not confirm a long-held belief that the flux in the past few million years has been higher by some 30–50% than the very long term average. Neither does our analysis confirm a hypothesis that the iron meteorite data indicate a ～150 million year periodicity in the cosmic ray flux, possibly related to variations in the long-term terrestrial climate.     

A compact, light-weight high data-rate antenna system for remote-sensing orbiters and space exploration

Upcoming space missions utilizing hyperspectral or other high-resolution sensors will generate a vast amount of data in orbit. The average communication duration between a spacecraft in low Earth orbit (LEO) to a dedicated ground station is short and in addition, due to the high amount of data to be transferred at link times, a high-performance communication system on board of the satellite is indispensable.A solution that provides longer acquisition times with the ground station is to employ a high data-rate inter-satellite link to a geostationary relay satellite, which requires a flat, compact, steerable, light-weight yet robust antenna. Such an antenna system (antenna module plus pointing module) was developed for S-Band at the Institute of Astronautics (Technische Universität München), in cooperation with German space companies, research institutes and the German Aerospace Center (DLR). Its successful operation via the geostationary relay satellite Artemis was demonstrated in cooperation with ESA in 2007.This paper describes the evaluation of an antenna system in the Ka-Band, as a successor to be developed in the next two years for high data rates and the various applications of such an antenna system.     

至臻至美——瓦尔特助力汽车工业

内燃机和变速箱是汽车行业的关键件.然而,内燃机和变速箱组件涉及大量复杂的机械加工流程,其复杂性与精密程度要求采用广泛多样的加工工艺.如果说制造业以生产率最大化为目标,则每项工艺必须赋予最佳的刀具解决方案.     

Towards a Unified View of Inhomogeneous Stellar Winds in Isolated Supergiant Stars and Supergiant High Mass X-Ray Binaries

Massive stars, at least \(\sim10\) times more massive than the Sun, have two key properties that make them the main drivers of evolution of star clusters, galaxies, and the Universe as a whole. On the one hand, the outer layers of massive stars are so hot that they produce most of the ionizing ultraviolet radiation of galaxies; in fact, the first massive stars helped to re-ionize the Universe after its Dark Ages. Another important property of massive stars are the strong stellar winds and outflows they produce. This mass loss, and finally the explosion of a massive star as a supernova or a gamma-ray burst, provide a significant input of mechanical and radiative energy into the interstellar space. These two properties together make massive stars one of the most important cosmic engines: they trigger the star formation and enrich the interstellar medium with heavy elements, that ultimately leads to formation of Earth-like rocky planets and the development of complex life. The study of massive star winds is thus a truly multidisciplinary field and has a wide impact on different areas of astronomy.In recent years observational and theoretical evidences have been growing that these winds are not smooth and homogeneous as previously assumed, but rather populated by dense “clumps”. The presence of these structures dramatically affects the mass loss rates derived from the study of stellar winds. Clump properties in isolated stars are nowadays inferred mostly through indirect methods (i.e., spectroscopic observations of line profiles in various wavelength regimes, and their analysis based on tailored, inhomogeneous wind models). The limited characterization of the clump physical properties (mass, size) obtained so far have led to large uncertainties in the mass loss rates from massive stars. Such uncertainties limit our understanding of the role of massive star winds in galactic and cosmic evolution.Supergiant high mass X-ray binaries (SgXBs) are among the brightest X-ray sources in the sky. A large number of them consist of a neutron star accreting from the wind of a massive companion and producing a powerful X-ray source. The characteristics of the stellar wind together with the complex interactions between the compact object and the donor star determine the observed X-ray output from all these systems. Consequently, the use of SgXBs for studies of massive stars is only possible when the physics of the stellar winds, the compact objects, and accretion mechanisms are combined together and confronted with observations.This detailed review summarises the current knowledge on the theory and observations of winds from massive stars, as well as on observations and accretion processes in wind-fed high mass X-ray binaries. The aim is to combine in the near future all available theoretical diagnostics and observational measurements to achieve a unified picture of massive star winds in isolated objects and in binary systems.