Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems.

Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. Grant numbers: 99-04-96017, 00-07-9011.     

Long clinostation influence on the ultrastructure of Funaria hygrometrica moss protonema cells

Changes in the ultrastructure of protonema cells of Funaria hygrometrica, cultivated during 20 days on a horizontal clinostat (2 rev/min), were determined by the electron microscopy method. About 20% of the cells were almost identical to those in the control, 20% were destructive cells, and in 60% ultrastructure changes were observed. The heterogeneity of the reaction demonstrated the evidence of sensitive cells on the clinostation process. Changes affected the ultrastructure of plastids, wall of the cell, and the form of the nucleus as well. Starch disappeared from chloroplasts practically completely, thylakoids swelled, granas frequently disappeared from plastids. Peroxisomes number in cells increased appreciably, width of cell walls decreased by almost half their size. Ca⁺⁺-binding sites were revealed in cytoplasma of cells. Electronocytochemical exposure of ATPases activity with the presence of Mg⁺⁺ and Ca⁺⁺ ions showed that Mg²⁺-ATPase activity localization in clinosted cells was not too different from the control, while Ca²⁺-ATPase location presented differences in plasmalemma and Ca-sites. These changes are perhaps connected with the membranes permeability breaking and affect the plant cells adaptation to the influence of hypogravitation.     

Transformation of a delta: Converting estuaries to lakes

In this century large-scale transformations have been carried out in the delta of the Rhine, Meuse and Scheldt in The Netherlands. The following principal consequences are considered: the loss of estuarine ecosystems, the change in the process of sedimentation and erosion, and the development of new aquatic and terrestrial ecosystems. The ecological consequences of the transformation of the Grevelingen estuary into a salt-water lake and some surprising developments are described briefly. The need for a limited restoration of the water exchange between the lake and the North Sea is indicated, as well as the major impacts of the exchange. The necessity for the maintenance or restoration of estuaries, the ecological development and the possibilities of the new lakes, and the management of these lakes by engineering structures are discussed. Careful land-use planning and ecosystem management, backed by research, are needed to make the fullest possible use of the potential offered by new lakes. It is also concluded that salt lakes have interesting perspectives.     

Attitude and Oribit Estimation Using Stars and Landmarks

An extended Kalman filter is used to process line-of-sight measurements to stars and known landmarks providing a statistical indication of performance in estimating spacecraft attitude, orbital ephemeris, and the bias drift of a set of three strapdown gyros. The landmark measurements were assumed to have been taken from the imagery of an Earth-observing multispectral scanner. It is shown that filtering of these noisy measurements results in highly accurate estimates of the above parameters. Results are given showing the sensitivity of performance to various system parameters such as star tracker accuracy, errors in the knowledge of landmark position, and number of stars and landmarks processed.     

Minimum Number of Satellites for Three-Dimensional Continuous Worldwide Coverage

Global positioning by means of satellites requires simultaneous observation by at least four satellites. The problem is to determine the minimum number of satellites and the corresponding orbital geometry necessary to satisfy this requirement on a continuous basis. To model the problem, a fixed number of users are assumed uniformly distributed in a known manner over the surface of the earth, and the satellites are restricted to exist in either three or four orbital planes. However, the orbit radius and inclination angle are left as variables. Under these assumptions, and starting with a small number of satellites which will be increased afterwards, an algorithm is developed to determine the visibility of satellites at each surface location. In this way it is possible to specify the minimum number of satellites needed by any desired orbital geometry. It is found that the number of satellites required for three-dimensional continuous worldwide coverage decreases as the orbit radius is increased. There appears to be no general trend regarding the effect of the inclination angle on the minimum number of satellites.     

Orbiting solar telescope on “Salyut-4” station

The orbiting solar telescope on Salyut-4 (F = 2,5 m, d = 250 mm) produces images of the Sun on the entrance slit of a stigmatic two-grating spectrograph (R₁ = 1 m, N₁ = 1200 lines/mm; R₂ = 0.5 m, N₂ = 2400 lines/mm, dispersion 16 Å/mm, spectral resolution 0,3 Å). The automatic system keeps the observed solar features on the slit of the spectrograph with an accuracy of 3–4 arc sec. The far UV-spectra (970–1400 Å) of solar flares, brightenings, flocculi and prominences were photographed and fresh coatings of mirrors were made during the flight.     

Microprocessor-Controlled Digital Shunt Regulator

A new approach to the design of power systems is presented in which a microprocessor is used as a controller for a digital shunt regulator (DSR). This approach meets the demands of future space and ground missions, i. e., high efficiency, high reliability, low weight, low volume, increased flexibility, and less development time. This approach responds to future demands by permitting realtime modification of system parameters for system optimization. This feature is especially important in the event of an anomaly. As the microprocessor need not be dedicated to the DSR, it can simultaneously be used for battery management and for charge regulator/discharge regulator control. This approach also reduces the component count, simplifies assembly and testing of the unit, results in significant time saving, and increases the reliability.     

Problems in the analysis of gamma-ray burst spectra

Spectral measurements by the Solar Maximum Mission have been used to confirm the cyclotron lines in gamma-ray bursts reported from the Konus experiment. We present ISEE-3 data for the same burst (GB800419) during the same period of time which show no line. We discuss various problems in the analysis of scintillator spectra and point out that unfolded spectra are not necessarily unique and that the position of a data point in a deconvolved spectrum may vary depending on the assumed overall shape of the spectrum. As a result, if the analysis assumes a soft spectrum (such as optically thin thermal bremsstrahlung) an absorption feature might appear, whereas a harder spectrum (such as a Comptonized blackbody) would not require the feature. Since the continuum shape probably changes during the duration of a typical burst, the nonuniqueness of the spectral unfolding, combined with the assumption that the continuum is optically thin thermal bremsstrahlung, could give rise to spurious “absorption” features which vary on a time scale of seconds. Despite these problems, there is still some evidence for $\text{narrow}$ spectral lines in the range 45 to 65 keV but not for the broad lines reported from the Konus experiment. Unfortunately, the range 45 to 65 keV is the most difficult spectral region to unfold.     

The sensitivity of the earth's radiation budget to changes in cloudiness

Cloudiness modulates the radiation budget at the top of the Earth-atmosphere system. For radiation balance studies, for climate diagnostic studies, and for climate modeling studies, it is important to know the sensitivity of both the outgoing longwave radiation and the net (absorbed solar minus outgoing longwave) radiation of the system to changes in cloudiness on a global basis. Based on a 45 month series of NOAA satellite scanning radiometer observations, estimates of the global distribution of these sensitivity parameters are obtained.