Injuries to plants from controlled environment contaminants.

The use of controlled environments is subject to problems from contaminants emitted from materials of the system and from plants. Many contaminants are difficult to identify because injurious dosages are very low, there is a lack of information on what compounds injure plants, because species and cultivars differ greatly in their sensitivity to injury and injury symptoms often are not distinctive. Plastics have been shown to emit many different volatile compounds. The compound, di-butyl phthalate, contained in certain flexible plastics, has been shown to be very toxic to plants. Other injuries have been produced by caulking compounds and bonded screening. Paints have been shown to release xylene that is toxic to plants. Steam for humidification can cause problems because of hydroxylamines and other compounds added to steam used for heating to control fungal growth in return lines. Mercury, from broken thermometers is a particular problem in growth chambers because small quantities can collect in cracks and slowly volatilize to slow growth of plants. Plants themselves release large quantities of volatile hydrocarbons, with ethylene being the commonly recognized chemical that can be damaging when allowed to accumulate. People release large quantities of carbon dioxide which can cause variations in the rate of growth of plants. Contaminant problems can be controlled through filtering of the air or ventilation with make-up air, however the potential for problems is always present and careful testing should be undertaken with the particular species and cultivars being grown insure that there are no toxic agents altering growth in each particular controlled environment being utilized.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Coarse attitude determination from Earth albedo measurements

Attitude estimation algorithms for the Thrusted Vector Mission which determine attitude based on Sun sensor and very coarse albedo sensor measurements are presented. On the basis of these measurements, it has been demonstrated by comparison with more accurate gyro-based attitude that it is possible to estimate three-axis attitude with an average error per axis of 11 deg. Most of this error is about the Sun direction. Both deterministic quick-look and optimal estimates are examined     

A large-scale perspective on ecosystems.

Interactions between ecological elements must be better understood in order to construct an ecological life support system in space. An index was devised to describe the complexity of material cyclings within a given ecosystem. It was then applied to the cyclings of bioelements in various systems of material cyclings including the whole Earth and national economies. The results show interesting characteristics of natural and man-made systems.     

Adaptive Forward Error Control for Digital Satellite Systems

Two hybrid schemes of time-frequency resource sharing to increase the rain margin of Ku-and Ka-band satellite systems are proposed. Scheme 1 requires sharing a small pool of bandwidth for adaptive forward error control coding, sharing a small pool of time frame for rate reduction, and sharing a portion of low frequency time-division multiple access (TDMA) back-up frame for downlink transmission to the rain affected stations. Scheme 2 utilizes variable rate modulation and forward error correction, shares a small pool of time frame for rate reduction, and shares a portion of low frequency TDMA back-up frame. Effective usable capacities of the system using these schemes are calculated. Distribution of resources in order to maximize the effective usable capacity is also analyzed. The results obtained are compared with other adaptive schemes. Preliminary analysis shows that the utilized capacity of scheme 1 exceeds 99 percent of the effective usable capacity possible if it never rains for an outage of 0.05 percent and fade margin of 2.5 dB. For scheme 2 similar performance is achievable at a fade margin of 1.5 dB. For higher outage objectives the loss of effective utilized capacity is higher for scheme 2.     

Chromosome instability of HPRT-mutant subclones induced by ionising radiation of various LET.

The induction of HPRT-mutations and survival of Chinese hamster cells (line B11ii-FAF28, clone 431) were studied after irradiation by 4He and 12C-ions of various LET (20-360 keV/micrometers), produced by the U-200 heavy ion accelerator. The RBE increases with LET up to the maximum at 100-200 keV/micrometers and then decreases. Cytogenetic analysis was performed on the HPRT-mutant subclones selected from unirradiated Chinese hamster V-79 cells and from HPRT-mutant subclones that arose after exposure to gamma-rays, 1 GeV protons and 14N-ions (LET-77 keV/micrometers), produced by the synchrophasotron and the U-400M heavy ion accelerator. Slow growing mutant subclones were observed. The cytogenetic properties of individual clones were highly heterogeneous and chromosome instability was observed in both spontaneous and radiation-induced mutants. Chromosome instability was highest among spontaneous mutants and decreased with increasing LET.     

Design of the TRIO system-on-chip for aerospace

Several design and testing aspects of the TRIO smart sensor data acquisition chip, developed by JHU/APL for NASA spacecraft applications are presented. TRIO includes a 10 bit self-corrected analog-to-digital converter (ADC), 16/32 analog inputs, a front end multiplexer with selectable aquisition time, a current source, memory, serial and parallel bus, and control logic. So far TRIO is used in many missions including Contour, Messenger, Stereo, Pluto, and the generic JPL X2000 spacecraft bus.     

Ginga observations of Seyfert galaxies

We observed twenty-eight Seyfert 2 galaxies with the Japanese X-ray satellite, Ginga, and found Seyfert 2 galaxies, in general, have the X-ray spectral characteristics of obscured Seyfert 1 nuclei. This result agrees with the predictions from Unified Seyfert model proposed by Antonucci and Miller /1/. However, among the observed Seyfert 2 galaxies, there are a few galaxies with no evidence of an obscuration, contrary to the general predictions of the unified model. We note that type 2 AGN will contribute to the Cosmic Diffuse X-ray Background, if the unified Seyfert model can be extended to the far distant AGN such as quasars.     

Controlled solar sail transfers into the near-sun orbits

We present results of numerical simulations of trajectories of a space probe with a flat solar sail which moves from a circular Earth orbit to near-Sun regions. We consider the upper limit of the sail temperature as the basic restriction. We also examine the planar (ecliptic) solar sail transfer with gravity-assist flybys around planets.     

The SOHO CELIAS/SEM EUV database from SC23 minimum to the present

The SOHO Solar EUV Monitor has been in operation since December 1995 onboard the SOHO spacecraft. This instrument is a highly stable transmission grating solar extreme ultraviolet spectrometer. It has made nearly continuous full disk solar irradiance measurements both within an 8 nm bandpass centered at 30.4 nm and throughout the 0.1 to 50 nm solar flux region since launch. The 30.4 nm flux, the 0.1 to 50 nm flux and the extracted soft X-ray (0.1 to 5 nm) flux are presented and compared with the behavior of solar proxies.