The effect of gravity on surface temperature and net photosynthetic rate of plant leaves.

To clarify the effects of gravity on heat/gas exchange between plant leaves and the ambient air, the leaf temperatures and net photosynthetic rates of plant leaves were evaluated at 0.01, 1.0, 1.5 and 2.0 G of 20 seconds each during a parabolic airplane flight. Thermal images of leaves were captured using infrared thermography at an air temperature of 26 degrees C, a relative humidity of 15% and an irradiance of 260 W m-2. The net photosynthetic rates were determined by using a chamber method with an infrared gas analyzer at an air temperature of 20 degrees C, a relative humidity of 50% and a photosynthetic photon flux of 0.5 mmol m-2 s-1. The mean leaf temperature increased by 1 degree C and the net photosynthetic rate decreased by 13% with decreasing gravity levels from 1.0 to 0.01 G. The leaf temperature decreased by 0.5 degree C and the net photosynthetic rate increased by 7% with increasing gravity levels from 1.0 to 2.0 G. Heat/gas exchanges between leaves and the ambient air were more retarded at lower gravity levels. A restricted free air convection under microgravity conditions in space would limit plant growth by retarding heat and gas exchanges between leaves and the ambient air.     

Host–parasite interactions in closed and open microbial cultivation system

The study addresses interaction of bacteria and phages in the host–parasite system in batch and continuous cultures. The study system consists of the auxotrophic strain of Brevibacterium – Brevibacterium sp. 22L – and the bacteriophage of Brevibacterium sp., isolated from the soil by the enrichment method.

1.

Closed system. In the investigation of the relationship between the time of bacterial lysis and multiplicity of phage infection it has been found that at a lower phage amount per cell it takes a longer time for the lysis of the culture to become discernible. Another important factor determining cytolysis in liquid medium is the physiological state of bacterial population. Specific growth rate of bacteria at the moment of phage infection has been chosen as an indicator of the physiological state of bacteria. It has been shown that the shortest latent period and the largest output of the phage are observed during the logarithmic growth phase of bacteria grown under favorable nutrient conditions. In the stationary phase, bacterial cells become “a bad host” for the phage, whose reproduction rate decreases, and the lysis either slows down significantly or does not occur at all.     

Current status of X-ray spectrometer development in the SELENE project

The X-ray spectrometer (XRS) on the SELENE (SELenological and ENgineering Explorer) spacecraft, XRS, will observe fluorescent X-rays from the lunar surface. The energy of the fluorescent X-ray depends on the elements of which the lunar soil consists, therefore we can determine elemental composition of the upper most lunar surface. The XRS consists of three components: XRF-A, SOL-B, and SOL-C. XRF-A is the main sensor to observe X-rays from the lunar surface. SOL-B is direct monitor of Solar X-ray using Si-PIN photodiode. SOL-C is another Solar X-ray monitor but observes the X-rays from the standard sample attached on the base plate. This enables us to analyze by a comparative method similar to typical laboratory XRF methods. XRF-A and SOL-C adopt charge coupled device as an X-ray detector which depletion layer is deep enough to detect X-rays. The X-ray spectra were obtained by the flight model of XRS components, and all components has been worked well to analyze fluorescent X-rays. Currently, development of the hardware and software of the XRS has been finished and we are preparing for system integration test for the launch.     

Remote upgrading of a space-borne instrument

The main purposes of experiment “Obstanovka” (“Environment” in Russian) consisting of several instruments are to measure a set of electromagnetic and plasma phenomena characterizing the space weather conditions, and to evaluate how such a big and highly energy consuming body as the International Space Station disturbs the surrounding plasma, and how the station itself is charged due to the operation of so many instruments, solar batteries, life supporting devices, etc. Two identical Langmuir electrostatic probes are included in the experiment “Obstanovka”. In this paper the Langmuir probes for “Obstanovka” experiment are described, including the choice of geometry (spherical or cylindrical), a more reliable method for the sweep voltage generation, an adaptive algorithm for the probe’s operation. Special attention is paid to the possibility for remote upgrading of the instrument from the ground using the standard communication channels.     

Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions

High-affinity Na⁺-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na⁺-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10 G, 1 h). The release of cytosolic l-[¹⁴C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[¹⁴C]glutamate in hypergravity as compared to control (4.8 ± 1.0% and 8.0 ± 1.0% of total label). Exocytotic release of l-[¹⁴C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[¹⁴C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0 ± 2.2% of total label in control to 35.0 ± 2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[¹⁴C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.     

TC-1和Geotail对亚暴过程中近地等离子体片磁扰动的联合观测

以2004年9月28日02:53:20 UT的亚暴为例, 通过TC-1在磁尾约12.5 R_e 和Geotail卫星在近地磁尾等离子体片约8～9 R_e的联合观测, 研究亚暴触发过程中近地磁尾等离子体片中等离子体波动特征. 结果表明, 亚暴触发区是近地磁尾中心等离子体片中较小的一个区域, 在亚暴触发区中低混杂不稳定性在近地磁尾等离子体片中存在, 准垂直传播的低混杂波发生在亚暴触发过程中, 而亚暴触发过程中近地磁尾等离子体片外边界区内的磁场偶极化信号和扰动都非常微弱. 在亚暴触发和亚暴膨胀相过程中出现了多次具有不同特征的磁场偶极化现象.     

Return to Europa: Overview of the Jupiter Europa orbiter mission

Missions to explore Europa have been imagined ever since the Voyager mission first suggested that Europa was geologically very young. Subsequently, the Galileo spacecraft supplied fascinating new insights into this satellite of Jupiter. Now, an international team is proposing a return to the Jupiter system and Europa with the Europa Jupiter System Mission (EJSM). Currently, NASA and ESA are designing two orbiters that would explore the Jovian system and then each would settle into orbit around one of Jupiter’s icy satellites, Europa and Ganymede. In addition, the Japanese Aerospace eXploration Agency (JAXA) is considering a Jupiter magnetospheric orbiter and the Russian Space Agency is investigating a Europa lander.     

Compact frequency standard using atoms trapped on a chip

We present a compact atomic frequency standard based on the interrogation of magnetically trapped ⁸⁷Rb atoms. Two photons, in the microwave and radiofrequency domain excite the atomic transition. At a magnetic field of 3.23 G this transition from ∣F = 1, m_F = −1〉 to ∣F = 2, m_F = 1〉 is 1st order insensitive to magnetic field variations. Long Ramsey interrogation times can thus be achieved, leading to a projected stability in the low 10⁻¹³ at 1 s. This makes this device a viable alternative to LITE and HORACE as a good candidate for replacing or complementing the rubidium frequency standards and passive hydrogen masers already on board of the GPS, GLONASS, and GALILEO satellites. Here we present preliminary results. We use an atom chip to cool and trap the atoms. A coplanar waveguide is integrated to the chip to carry the Ramsey interrogation signal, making the physics package potentially as small as (5 cm)³. We describe the experimental apparatus and show preliminary Ramsey fringes of 1.25 Hz linewidth. We also show a preliminary frequency stability σ_y = 1.5 × 10⁻¹²τ^−1/2 for 10 < τ < 10³ s. This represents one order of magnitude improvement with respect to previous experiments.