Effects of microgravity on c-fos gene expression in osteoblast-like MC3T3-E1 cells.

The paper summarizes the data on proliferation and gravity-related gene expression of osteoblasts that were obtained from an experiment conducted under simulated and real microgravity conditions. Simulated microgravity conditions obtained in a clinostat depress proliferation of both osteoblast-like MC3T3-E1 and HeLa carcinoma cells. This depression of proliferation occurs in a collagen gel culture in which the flow of culture medium by rotation may be reduced. Interestingly, MC3T3-E1 cells which are probably one of target cells to microgravity are more sensitive than the HeLa cells. Simulated microgravity inhibited the epidermal growth factor (EGF)-induced c-fos gene expression in the MC3T3-El cells. To examine in detail the effect of real microgravity on the EGF signal transduction cascade in osteoblasts, MC3T3-E1 cells were cultured in the Cell Culture Experiment Module of the sounding rocket TR-1A6. The EGF-induced c-fos expression in cells was depressed under short-term microgravity conditions in the sounding rocket, while the phosphorylation of mitogen-activated protein kinase (MAPK) was not affected compared with the controls grown on the ground. These results suggest that an action site of microgravity in the signal transduction pathway may be downstream of MAPK.     

Beagle 2: a proposed exobiology lander for ESA's 2003 Mars Express mission.

The aim of the proposed Beagle 2 small lander for ESA's 2003 Mars Express mission is to search for organic material on and below the surface of Mars and to study the inorganic chemistry and mineralogy of the landing site. The lander will have a total mass of 60kg including entry, descent, and landing system. Experiments will be deployed on the surface using a robotic arm. It will use a mechanical mole and grinder to obtain samples from below the surface, under rocks, and inside rocks. Sample analysis by a mass spectrometer will include isotopic analysis. An optical microscope, an X-ray spectrometer and a Mossbauer spectrometer will conduct in-situ rock studies.     

湿热对单向复合材料层合板Ⅱ型分层特性的影响

利用横向裂纹拉伸试验模型（Transverse Crack Tension-tension Test）,在3种湿热环境（室温、85℃和85℃/95%RH）条件下,对2种单向碳纤维增强树脂基复合材料（T300/QY8911和HTA/6376）层合板的Ⅱ型分层特性进行了试验研究。结果表明湿热环境导致复合材料层间断裂韧性降低,引起Ⅱ型应变能释放率门槛值显着下降,裂纹扩展速率大大提高,温度的影响较湿度更为明显。利用电镜（SEM）对试样分层表面进行了检测,湿度对断面特征无明显的影响。     

The Solar Isotope Spectrometer for the Advanced Composition Explorer

The Solar Isotope Spectrometer (SIS), one of nine instruments on the Advanced Composition Explorer (ACE), is designed to provide high- resolution measurements of the isotopic composition of energetic nuclei from He to Zn (Z=2 to 30) over the energy range from ∼10 to ∼100 MeV nucl−1. During large solar events SIS will measure the isotopic abundances of solar energetic particles to determine directly the composition of the solar corona and to study particle acceleration processes. During solar quiet times SIS will measure the isotopes of low-energy cosmic rays from the Galaxy and isotopes of the anomalous cosmic-ray component, which originates in the nearby interstellar medium. SIS has two telescopes composed of silicon solid-state detectors that provide measurements of the nuclear charge, mass, and kinetic energy of incident nuclei. Within each telescope, particle trajectories are measured with a pair of two-dimensional silicon-strip detectors instrumented with custom, very large-scale integrated (VLSI) electronics to provide both position and energy-loss measurements. SIS was especially designed to achieve excellent mass resolution under the extreme, high flux conditions encountered in large solar particle events. It provides a geometry factor of ∼40 cm2 sr, significantly greater than earlier solar particle isotope spectrometers. A microprocessor controls the instrument operation, sorts events into prioritized buffers on the basis of their charge, range, angle of incidence, and quality of trajectory determination, and formats data for readout by the spacecraft. This paper describes the design and operation of SIS and the scientific objectives that the instrument will address. This revised version was published online in June 2006 with corrections to the Cover Date.     

Direction-finding with sparse rectangular dual-size spatialinvariance array

A novel sparse array geometry embedding two sizes of spatial invariances is presented for use with a new ESPRIT-based (estimation of signal parameters via rotational invariance techniques) algorithm for aperture extension. The half-wavelength invariance yields unambiguous but high-variance direction cosine estimates to disambiguate low-variance but cyclically ambiguous estimates from the larger invariance. With larger invariance at 60 half-wavelengths, resolution threshold for two closely spaced emitters is reduced by 50 dB and estimation error by 100-fold. Array design formulas are also presented     

Improvement of forebody/inlet integration for hypersonic vehicle

Results of a numerical (CFD) study of the influence of the forebody shape on local flow parameters at a bottom-mounted inlet entrance are presented. The free-stream Mach number is assumed to be 3.5–7.0. Some recommendations on forebody shape optimization are provided. Main characteristics of the air inlet are evaluated.     

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.     

Neurobiological problems in long-term deep space flights.

Future missions in space may involve long-term travel beyond the magnetic field of the Earth, subjecting astronauts to radiation hazards posed by solar flares and galactic cosmic rays, altered gravitation fields and physiological stress. Thus, it is critical to determine if there will be any reversible or irreversible, detrimental neurological effects from this prolonged exposure to space. A question of particular importance focuses on the long-term effects of the space environment on the central nervous system (CNS) neuroplasticity, with the potential acute and/or delayed effects that such perturbations might entail. Although the short-term effects of microgravity on neural control were studied on previous low earth orbit missions, the late consequences of stress in space, microgravity and space radiation have not been addressed sufficiently at the molecular, cellular and tissue levels. The possibility that space flight factors can interact influencing the neuroplastic response in the CNS looms critical issue not only to understand the ontogeny of the CNS and its functional integrity, but also, ultimately the performance of astronauts in extended space forays. The purpose of this paper is to review the neurobiological modifications that occur in the CNS exposed to the space environment, and its potential consequences for extended deep space flight.     

航空发动机用金属橡胶隔振器动静态性能的研究

以某型号航空发动机阻尼减振需求为背景,对开发研制的金属橡胶隔振器与现用的橡胶隔振器进行了动、静态实验研究。研究结果表明:金属橡胶隔振器的能量耗散性能、静态承载能力、过临界的能力及提供振动防护的区域都远远大于橡胶隔振器,而且通过改变金属橡胶隔振器的结构参数和预压缩量可以优化其隔振效果。研究结果为利用金属橡胶隔振器来改善航空发动机的振动状况提供了依据。     

Study of physiological effects of weightlessness and artificial gravity in the flight of the biosatellite Cosmos-936.

In the 18.5-day flight of the Soviet biosatellite Cosmos-936 (3-22, August 1977) com-parative investigations of the physiological effects of prolonged weightlessness (20 rats) and artificial gravity of 1 g (10 rats) were carried out. Throughout the flight artificial gravity was generated by means of animal rotation in two centrifuges with a radius of 320mm. Postflight examination of animals and treatment of the flight data were performed by Soviet scientists in collaboration with the specialists from Bulgaria, Czechoslovakia, the German Democratic Republic, Hungary, Poland, Rumania, France and the U.S.A. During the flight the total motor activity of the weightless rats was higher and their body temperature was lower than those of the centrifuged animals. Postflight examination of the weightless rats showed a greater percentage of errors during maze an increase in water intake and a decrease in diuresis; a fall of the resistance of peripheral red cells; an increase in the conditionally pathogenic microflora in the mouth; a decrease of oxygen consumption, carbon dioxide production and energy expenditures; a drop in the static physical endurance; a decline in the capacity to keep balance on the rail; an increase in the latent period of the lifting reflex, etc. The centrifugal animals displayed lesser or no change of the above type. These findings together with the biochemical and morphological data give evidence that during and after flight adaptive processes in the centrifuged rats developed better.