New instrumentation for optical measuring of oxygen in gas or dissolved in liquids.

The optical oxygen sensor is a novel device for the determination of oxygen in gases or dissolved in liquids. It is based on the measurement principle of fluorescence quenching, which is completely different from that of polarographic oxygen sensors (today the most widespread devices of oxygen detection). The new instrument offers features and advantages, which render it not only a realistic alternative, but, for specific applications, make it superior to existing electrochemical methods. The system is based on low-cost semiconductor devices (light-emitting diodes, photodiodes, low-cost analogue and digital components) and new LED-compatible oxygen-sensitive membranes. The flow cell of the instrument may be thermostatted and the sensor can be calibrated by a simple two-point calibration procedure. The optical oxygen sensor is particularly suitable for measuring dissolved oxygen in respirometry, since no oxygen is consumed by the device and the signal is independent of sample flowrate or stirring speed. Typical fields of application are monitoring of oxygen in ground and drinking water, in process control in bioreactors and in breath gas and blood gas analysis.     

Use of combined light flash and plasma measurements to study hypervelocity impact processes

We present new measurements concerning generation of light flash during hypervelocity impacts. We use iron particles (10⁻¹³ to 10⁻¹⁷ kg) with velocities over the range 1 to 42 km/s impacting semi-infinite targets (aluminium and molybdenum). The main results of previous work in the field are found to be reproduced with some slight deviations. For iron projectiles with given mass and velocity the energy of the flash (normalized to mass) is proportional to velocity to the power of 3.5 for aluminium targets and 3.9 for molybdenum targets. The duration of the flash is of order 1 microsecond. Simultaneous measurements of the generation of impact plasma do not change this. The onset of plasma generation of the bulk target material does not affect the total light flash energy. We discuss the duration of the flash compared to a simple calculation of temperature in the target and plasma vs time.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

An introduction to gravity perception in plants and fungi--a multiplicity of mechanisms.

The origin and subsequent evolution of life on Earth has taken place within an environment of which a 1g gravitational force is a part. Thus, all living organisms accommodate this variable in their structure and function. Evolution has also selected mechanisms to sense gravity which, in consequence, give particular orientations to living process. It is anticipated that the higher the evolutionary status of an organism, the greater the chance that it will possess multiple mechanisms of gravisensing because evolution discards nothing that assists fitness, but only adds to existing processes. A multiplicity of mechanisms permits gravity to participate in a wide range of developmental programmes, such as taxes, morphisms and tropisms, through the action of different sensors and distinct transduction/response pathways.     

Aiming at a 1-cm Orbit for Low Earth Orbiters: Reduced-Dynamic and Kinematic Precise Orbit Determination

The computation of high-accuracy orbits is a prerequisite for the success of Low Earth Orbiter (LEO) missions such as CHAMP, GRACE and GOCE. The mission objectives of these satellites cannot be reached without computing orbits with an accuracy at the few cm level. Such a level of accuracy might be achieved with the techniques of reduced-dynamic and kinematic precise orbit determination (POD) assuming continuous Satellite-to-Satellite Tracking (SST) by the Global Positioning System (GPS). Both techniques have reached a high level of maturity and have been successfully applied to missions in the past, for example to TOPEX/POSEIDON (T/P), leading to (sub-)decimeter orbit accuracy. New LEO gravity missions are (to be) equipped with advanced GPS receivers promising to provide very high quality SST observations thereby opening the possibility for computing cm-level accuracy orbits. The computation of orbits at this accuracy level does not only require high-quality GPS receivers, but also advanced and demanding observation preprocessing and correction algorithms. Moreover, sophisticated parameter estimation schemes need to be adapted and extended to allow the computation of such orbits. Finally, reliable methods need to be employed for assessing the orbit quality and providing feedback to the different processing steps in the orbit computation process. This revised version was published online in August 2006 with corrections to the Cover Date.     

The role of hydration and radiation quality in the induction of DNA damage--chemical aspects.

The mutagenic and lethal effects of ionising radiation are thought to result from chemical modifications induced within DNA. This DNA damage is significantly influenced by the chemical environment and the radiation quality (LET). Water closely associated with the DNA and its immediate environment is involved in the early chemical pathways which lead to the induction of DNA damage and is reflected in the cellular radiosensitivity. For instance, hydration of DNA influences hole migration leading to its localisation at guanine. Changes in the radiation quality are discussed in terms of the complexity of the radical clusters produced. It is inferred that at higher LET, the influence of the chemical environment (O2 etc) decreases with respect to DNA damage and cellular radiosensitivity. It is therefore important to include these effects of environment of the DNA upon the early chemical pathways in models of radiation action.     

Collisions in space: A retrospective overview of ISAS studies

A chronological review of studies in ISAS concerning collisions in space is presented. The collision probability in space with artificial orbiting bodies was estimated, and a Space Traffic Control System was proposed, in 1971. The design of a space station for safety against collision hazards was discussed in 1972. A trajectory optimization technique for low-thrust multiple rendezvous mission in order ti sweep space debris around the earth was developed in 1977. In 1984, the collision probability was reestimated using space bedris data accumulated for more than a decade. Several experimental projects in ISAS, such as hypervelocity impact experiments using a railgun system, sampling and measuring of alumina particles in exhaust plume of solid-propellant propellant rocket motors, and a result of analysis on the behavior of such alumina particles in orbit are also introduced.     

Coarse attitude determination from Earth albedo measurements

Attitude estimation algorithms for the Thrusted Vector Mission which determine attitude based on Sun sensor and very coarse albedo sensor measurements are presented. On the basis of these measurements, it has been demonstrated by comparison with more accurate gyro-based attitude that it is possible to estimate three-axis attitude with an average error per axis of 11 deg. Most of this error is about the Sun direction. Both deterministic quick-look and optimal estimates are examined     

Adaptive Forward Error Control for Digital Satellite Systems

Two hybrid schemes of time-frequency resource sharing to increase the rain margin of Ku-and Ka-band satellite systems are proposed. Scheme 1 requires sharing a small pool of bandwidth for adaptive forward error control coding, sharing a small pool of time frame for rate reduction, and sharing a portion of low frequency time-division multiple access (TDMA) back-up frame for downlink transmission to the rain affected stations. Scheme 2 utilizes variable rate modulation and forward error correction, shares a small pool of time frame for rate reduction, and shares a portion of low frequency TDMA back-up frame. Effective usable capacities of the system using these schemes are calculated. Distribution of resources in order to maximize the effective usable capacity is also analyzed. The results obtained are compared with other adaptive schemes. Preliminary analysis shows that the utilized capacity of scheme 1 exceeds 99 percent of the effective usable capacity possible if it never rains for an outage of 0.05 percent and fade margin of 2.5 dB. For scheme 2 similar performance is achievable at a fade margin of 1.5 dB. For higher outage objectives the loss of effective utilized capacity is higher for scheme 2.