Thyrotropin receptor and membrane interactions in FRTL-5 thyroid cell strain in microgravity

The aim of this work was to analyze the possible alteration of thyrotropin (TSH) receptors in microgravity, which could explain the absence of thyroid cell proliferation in the space environment. Several forms of the TSH receptor are localized on the plasma membrane associated with caveolae and lipid rafts. The TSH regulates the fluidity of the cell membrane and the presence of its receptors in microdomains that are rich in sphingomyelin and cholesterol. TSH also stimulates cyclic adenosine monophosphate (cAMP) accumulation and cell proliferation. Reported here are the results of an experiment in which the FRTL-5 thyroid cell line was exposed to microgravity during the Texus-44 mission (launched February 7, 2008, from Kiruna, Sweden). When the parabolic flight brought the sounding rocket to an altitude of 264?km, the culture media were injected with or without TSH in the different samples, and weightlessness prevailed on board for 6 minutes and 19 seconds. Control experiments were performed, in parallel, in an onboard 1g centrifuge and on the ground in Kiruna laboratory. Cell morphology and function were analyzed. Results show that in microgravity conditions the cells do not respond to TSH treatment and present an irregular shape with condensed chromatin, a modification of the cell membrane with shedding of the TSH receptor in the culture medium, and an increase of sphingomyelin-synthase and Bax proteins. It is possible that real microgravity induces a rearrangement of specific sections of the cell membrane, which act as platforms for molecular receptors, thus influencing thyroid cell function in astronauts during space missions.     

Sunspot dynamics are reflected in human physiology and pathophysiology

Periodic episodes of increased sunspot activity (solar electromagnetic storms) occur with 10-11 and 5-6 year periodicities and may be associated with measurable biological events. We investigated whether this sunspot periodicity characterized the incidence of Pap smear-determined cervical epithelial histopathologies and human physiologic functions. From January 1983 through December 2003, monthly averages were obtained for solar flux and sunspot numbers; six infectious, premalignant and malignant changes in the cervical epithelium from 1,182,421 consecutive, serially independent, screening Pap smears (59°9″N, 4°29″E); and six human physiologic functions of a healthy man (oral temperature, pulse, systolic and diastolic blood pressure, respiration, and peak expiratory flow), which were measured ～5 times daily during ～34,500 self-measurement sessions (44°56″N, 93°8″W). After determining that sunspot numbers and solar flux, which were not annually rhythmic, occurred with a prominent 10-year and a less-prominent 5.75-year periodicity during this 21-year study span, each biological data set was analyzed with the same curve-fitting procedures. All six annually rhythmic Pap smear-detected infectious, premalignant and malignant cervical epithelial pathologies showed strong 10-year and weaker 5.75-year cycles, as did all six self-measured, annually rhythmic, physiologic functions. The phases (maxima) for the six histopathologic findings and five of six physiologic measurements were very near, or within, the first two quarters following the 10-year solar maxima. These findings add to the growing evidence that solar magnetic storm periodicities are mirrored by cyclic phase-locked rhythms of similar period length or lengths in human physiology and pathophysiology.     

On the stabilization of ribose by silicate minerals

The RNA-world theory hypothesizes that early Earth life was based on the RNA molecule. However, the notion that ribose, the sugar in RNA, is unstable still casts a serious doubt over this theory. Recently, it has been found that the silicate-mediated formose reaction facilitates the stabilization of ribose. Using accurate quantum chemical calculations, we determined the relative stability of the silicate complexes of arabinose, lyxose, ribose, and xylose with the intent to determine which would form predominantly from a formose-like reaction. Five stereoisomers were investigated for each complex. The stereoisomers of 2:1 ribose-silicate are the more stable ones, to the extent that the least stable of these is even more stable than the most stable stereoisomer of the other 2:1 sugar-silicate complexes. Thus, thermodynamically, a formose-like reaction in the presence of silicate minerals should preferentially form the silicate complex of ribose over the silicate complex of arabinose, lyxose, and xylose.     

Adsorption of amino acids (ALA, CYS, HIS, MET) on zeolites: fourier transform infrared and Raman spectroscopy investigations

Minerals adsorb more amino acids with charged R-groups than amino acids with uncharged R-groups. Thus, the peptides that form from the condensation of amino acids on the surface of minerals should be composed of amino acid residues that are more charged than uncharged. However, most of the amino acids (74%) in today's proteins have an uncharged R-group. One mechanism with which to solve this paradox is the use of organophilic minerals such as zeolites. Over the range of pH (pH 2.66-4.50) used in these experiments, the R-group of histidine (His) is positively charged and neutral for alanine (Ala), cysteine (Cys), and methionine (Met). In acidic hydrothermal environments, the pH could be even lower than those used in this study. For the pH range studied, the zeolites were negatively charged, and the overall charge of all amino acids was positive. The conditions used here approximate those of prebiotic Earth. The most important finding of this work is that the relative concentrations of each amino acid (X=His, Met, Cys) to alanine (X/Ala) are close to 1.00. This is an important result with regard to prebiotic chemistry because it could be a solution for the paradox stated above. Pore size did not affect the adsorption of Cys and Met on zeolites, and the Si/Al ratio did not affect the adsorption of Cys, His, and Met. ZSM-5 could be used for the purification of Cys from other amino acids (Student-Newman-Keuls test, p<0.05), and mordenite could be used for separation of amino acids from each other (Student-Newman-Keuls test, p<0.05). As shown by Fourier transform infrared (FT-IR) spectra, Ala interacts with zeolites through the [Formula: see text] group, and methionine-zeolite interactions involve the COO, [Formula: see text], and CH(3) groups. FT-IR spectra show that the interaction between the zeolites and His is weak. Cys showed higher adsorption on all zeolites; however, the hydrophobic Van der Waals interaction between zeolites and Cys is too weak to produce any structural changes in the Cys groups (amine, carboxylic, sulfhydryl, etc.); thus, the FT-IR and Raman spectra are the same as those of solid Cys.     

Self-consistent Simulations of Alfv��n Wave Driven Winds from the Sun and Stars

We review our recent results of Alfvén wave-driven winds. First, we present the result of self-consistent 1D MHD simulations for solar winds from the photosphere to interplanetary region. Here, we emphasize the importance of the reflection of Alfvén waves in the density stratified corona and solar winds. We also introduce the recent Hinode observation that might detect the reflection signature of transverse (Alfvénic) waves by Fujimura and Tsuneta (Astrophys. J. 702:1443, 2009). Then, we show the results of Alfvén wave-driven winds from red giant stars. As a star evolves to the red giant branch, the properties of stellar winds drastically change from steady coronal winds to intermittent chromospheric winds. We also discuss how the stellar evolution affects the wave reflection in the stellar atmosphere and similarities and differences of accretion disk winds by MHD turbulence.     

Deducing Electron Properties from Hard X-ray Observations

X-radiation from energetic electrons is the prime diagnostic of flare-accelerated electrons. The observed X-ray flux (and polarization state) is fundamentally a convolution of the cross-section for the hard X-ray emission process(es) in question with the electron distribution function, which is in turn a function of energy, direction, spatial location and time. To address the problems of particle propagation and acceleration one needs to infer as much information as possible on this electron distribution function, through a deconvolution of this fundamental relationship. This review presents recent progress toward this goal using spectroscopic, imaging and polarization measurements, primarily from the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI). Previous conclusions regarding the energy, angular (pitch angle) and spatial distributions of energetic electrons in solar flares are critically reviewed. We discuss the role and the observational evidence of several radiation processes: free-free electron-ion, free-free electron-electron, free-bound electron-ion, photoelectric absorption and Compton backscatter (albedo), using both spectroscopic and imaging techniques. This unprecedented quality of data allows for the first time inference of the angular distributions of the X-ray-emitting electrons and improved model-independent inference of electron energy spectra and emission measures of thermal plasma. Moreover, imaging spectroscopy has revealed hitherto unknown details of solar flare morphology and detailed spectroscopy of coronal, footpoint and extended sources in flaring regions. Additional attempts to measure hard X-ray polarization were not sufficient to put constraints on the degree of anisotropy of electrons, but point to the importance of obtaining good quality polarization data in the future.     

霍尔推力器等离子体磁鞘特性

为进一步研究霍尔推力器壁面二次电子发射对发动机性能的影响,建立流体模型数值模拟了霍尔推力器磁化二次电子等离子体的鞘层特性,采用Sagdeev势的方法得到了鞘层的玻姆判据,以这个判据为鞘层边界条件数值讨论了磁场、二次电子发射以及不同等离子体对推力器等离子体鞘层结构的影响。结果表明,磁场和二次电子发射系数的增加都将使鞘层中粒子密度增加,鞘层电势升高,鞘层厚度减小;对于氩和氙二种等离子体,氙等离子体产生的鞘层势垒高,粒子密度大,鞘层厚度增加。这些结果直接影响推力器的电子传导机制及性能。     

Optimization of the centrifugal impeller flow passage in high-speed pumps

Based on the jet-vortex model for flow passage hydrodynamics in a high-speed centrifugal pump with the open-type impeller, we present the calculated relations for design and optimization of the experimentally verified pump design parameters.     

System sensitivity to parametric variations in the problem of lateral motion flight vehicle control

The results of investigation of dynamic system sensitivity to parametric variations in the channel of flight vehicle lateral motion are presented using the notion of infinitesimal transformation. The exact relationships establishing a link between the structure of parameter deviation and that of system phase coordinate deviation are obtained.     

Improved rainfall estimation over the Indian region using satellite infrared technique

The GOES Precipitation Index (GPI) technique (Arkin, 1979) for rainfall estimation has been in operation for the last three decades. However, its applications are limited to the larger temporal and spatial scales. The present study focuses on the augmentation on GPI technique by incorporating a moisture factor for the environmental correction developed by Vicente et al. (1998). It consists of two steps; in the first step the GPI technique is applied to the Kalpana-IR data for rainfall estimation over the Indian land and oceanic region and in the second step an environmental moisture correction factor is applied to the GPI-based rainfall to estimate the final rainfall. Detailed validation with rain gauges and comparison with Tropical Rainfall Measuring Mission (TRMM) merged data product (3B42) are performed and it is found that the present technique is able to estimate the rainfall with better accuracy than the GPI technique over higher temporal and spatial domains for many operational applications in and around the Indian regions using Indian geostationary satellite data. Further comparison with the Doppler Weather Radar shows that the present technique is able to retrieve the rainfall with reasonably good accuracy.