Lichens survive in space: results from the 2005 LICHENS experiment

This experiment was aimed at establishing, for the first time, the survival capability of lichens exposed to space conditions. In particular, the damaging effect of various wavelengths of extraterrestrial solar UV radiation was studied. The lichens used were the bipolar species Rhizocarpon geographicum and Xanthoria elegans, which were collected above 2000 m in the mountains of central Spain and as endolithic communities inhabiting granites in the Antarctic Dry Valleys. Lichens were exposed to space in the BIOPAN-5 facility of the European Space Agency; BIOPAN-5 is located on the outer shell of the Earth-orbiting FOTON-M2 Russian satellite. The lichen samples were launched from Baikonur by a Soyuz rocket on May 31, 2005, and were returned to Earth after 16 days in space, at which time they were tested for survival. Chlorophyll fluorescence was used for the measurement of photosynthetic parameters. Scanning electron microscopy in back-scattered mode, low temperature scanning electron microscopy, and transmission electron microscopy were used to study the organization and composition of both symbionts. Confocal laser scanning microscopy, in combination with the use of specific fluorescent probes, allowed for the assessment of the physiological state of the cells. All exposed lichens, regardless of the optical filters used, showed nearly the same photosynthetic activity after the flight as measured before the flight. Likewise, the multimicroscopy approach revealed no detectable ultrastructural changes in most of the algal and fungal cells of the lichen thalli, though a greater proportion of cells in the flight samples had compromised membranes, as revealed by the LIVE/DEAD BacLight Bacterial Viability Kit. These findings indicate that most lichenized fungal and algal cells can survive in space after full exposure to massive UV and cosmic radiation, conditions proven to be lethal to bacteria and other microorganisms. The lichen upper cortex seems to provide adequate protection against solar radiation. Moreover, after extreme dehydration induced by high vacuum, the lichens proved to be able to recover, in full, their metabolic activity within 24 hours.     

Spread-F occurrence during geomagnetic storms near the southern crest of the EIA in Argentina

This work presents, for the first time, the analysis of the occurrence of ionospheric irregularities during geomagnetic storms at Tucumán, Argentina, a low latitude station in the Southern American longitudinal sector (26.9°S, 294.6°E; magnetic latitude 15.5°S) near the southern crest of the equatorial ionization anomaly (EIA). Three geomagnetic storms occurred on May 27, 2017 (a month of low occurrence rates of spread-F), October 12, 2016 (a month of transition from low to high occurrence rates of spread-F) and November 7, 2017 (a month of high occurrence rates of spread-F) are analyzed using Global Positioning System (GPS) receivers and ionosondes. The rate of change of total electron content (TEC) Index (ROTI), GPS Ionospheric L-band scintillation, the virtual height of the F-layer bottom side (h'F) and the critical frequency of the F2 layer (foF2) are considered. Furthermore, each ionogram is manually examined for the presence of spread-F signatures.The results show that, for the three events studied, geomagnetic activity creates favorable conditions for the initiation of ionospheric irregularities, manifested by ionogram spread-F and TEC fluctuation. Post-midnight irregularities may have occurred due to the presence of eastward disturbance dynamo electric fields (DDEF). For the May storm, an eastward over-shielding prompt penetration electric field, (PPEF) is also acting. A possibility is that the PPEF is added to the DDEF and produces the uplifting of the F region that helps trigger the irregularities. Finally, during October and November, strong GPS L band scintillation is observed associated with strong range spread-F (SSF), that is, irregularities extending from the bottom-side to the topside of the F region.     

MICROSCOPE On-ground and In-orbit Calibration

The MICROSCOPE mission, to be launched in 2011, will perform the test of the universality of free fall (Equivalence Principle) to an accuracy of 10^?15. The payload consists of two sensors, each controlling the free fall of a pair of test masses: the first for the test of the Equivalence Principle (titanium/platinum), the second for performance verification (platinum/platinum). The capability to detect a faint violation signal of the EP test is conditioned upon the rejection of disturbances arising from the coupling and misalignments of the instrument vectorial outputs. Therefore the performance of the mission depends on the success of the series of calibration operations which are planned during the satellite life in orbit. These operations involve forced motion of the masses with respect to the satellite. Specific data processing tools and simulations are integral parts of the calibration and performance enhancement process, as are the tests operated on ground at the ZARM drop tower. The presentation will focus on the current status of the MICROSCOPE payload, the rationale for the in-orbit calibrations, the data processing operations and the tests performed at the ZARM drop tower.     

Statistical evaluation of GLONASS amplitude scintillation over low latitudes in the Brazilian territory

The ionospheric scintillation, generated by the ionospheric plasma irregularities, affects the radio signals that pass through it. Their effects are widely studied in the literature with two different approaches. The first one deals with the use of radio signals to study and understand the morphology of this phenomenon, while the second one seeks to understand and model how much this phenomenon interferes in the radio signals and consequently in the services to which these systems work. The interest of several areas, particularly to those that are life critical, has increased using the concept of satellite multi-constellation, which consists of receiving, processing and using data from different navigation and positioning systems. Although there is a vast literature analyzing the effects of ionospheric scintillation on satellite navigation systems, the number of studies using signals received from the Russian satellite positioning system (named GLONASS) is still very rare. This work presents for the first time in the Brazilian low-latitude sector a statistical analysis of ionospheric scintillation data for all levels of magnetic activities obtained by a set of scintillation monitors that receive signals from the GLONASS system. In this study, data collected from four stations were used in the analysis; Fortaleza, Presidente Prudente, São José dos Campos and Porto Alegre. The GLONASS L-band signals were analyzed for the period from December 21, 2012 to June 20, 2016, which includes the peak of the solar cycle 24 that occurred in 2014. The main characteristics of scintillation presented in this study include: (1) the statistical evaluation of seasonal and solar activity, showing the chances that an user on similar geophysical conditions may be susceptible to the effects of ionospheric scintillation; (2) a temporal analysis based on the local time distribution of scintillation at different seasons and intensity levels; and (3) the evaluation of number of simultaneously affected channels and its effects on the dilution of precision (DOP) for GNSS users are also presented in order to alert the timetables in which navigation will be most susceptible to such effects, as well as statistics on simultaneously affected channels. Relevant results about these statistical characteristics of scintillation are presented and analyzed providing relevant information about availability of a navigation system.     

Status of the Stratospheric Observatory for Infrared Astronomy (SOFIA)

The Stratospheric Observatory for Infrared Astronomy (SOFIA), a joint US/German project, is a 2.5-m infrared airborne telescope carried by a Boeing 747-SP that flies in the stratosphere at altitudes as high as 45,000 ft (13.72 km). This facility is capable of observing from 0.3 μm to 1.6 mm with an average transmission greater than 80% averaged over all wavelengths. SOFIA will be staged out of the NASA Dryden Flight Research Center aircraft operations facility at Palmdale, CA. The SOFIA Science Mission Operations (SMO) will be located at NASA Ames Research Center, Moffett Field, CA. First science flights began in 2010 and a full operations schedule of up to one hundred 8 to 10 hour-long flights per year will be reached by 2014. The observatory is expected to operate until the mid-2030s. SOFIA’s initial complement of seven focal plane instruments includes broadband imagers, moderate-resolution spectrographs that will resolve broad features due to dust and large molecules, and high-resolution spectrometers capable of studying the kinematics of atomic and molecular gas at sub-km/s resolution. We describe the SOFIA facility and outline the opportunities for observations by the general scientific community and for future instrumentation development. The operational characteristics of the SOFIA first-generation instruments are summarized. The status of the flight test program is discussed and we show First Light images obtained at wavelengths from 5.4 to 37 μm with the FORCAST imaging camera. Additional information about SOFIA is available at http://www.sofia.usra.edu and http://www.sofia.usra.edu/Science/docs/SofiaScienceVision051809-1.pdf.     

SOLID3: a multiplex antibody microarray-based optical sensor instrument for in situ life detection in planetary exploration

The search for unequivocal signs of life on other planetary bodies is one of the major challenges for astrobiology. The failure to detect organic molecules on the surface of Mars by measuring volatile compounds after sample heating, together with the new knowledge of martian soil chemistry, has prompted the astrobiological community to develop new methods and technologies. Based on protein microarray technology, we have designed and built a series of instruments called SOLID (for "Signs Of LIfe Detector") for automatic in situ detection and identification of substances or analytes from liquid and solid samples (soil, sediments, or powder). Here, we present the SOLID3 instrument, which is able to perform both sandwich and competitive immunoassays and consists of two separate functional units: a Sample Preparation Unit (SPU) for 10 different extractions by ultrasonication and a Sample Analysis Unit (SAU) for fluorescent immunoassays. The SAU consists of five different flow cells, with an antibody microarray in each one (2000 spots). It is also equipped with an exclusive optical package and a charge-coupled device (CCD) for fluorescent detection. We demonstrated the performance of SOLID3 in the detection of a broad range of molecular-sized compounds, which range from peptides and proteins to whole cells and spores, with sensitivities at 1-2?ppb (ng?mL?1) for biomolecules and 10? to 103 spores per milliliter. We report its application in the detection of acidophilic microorganisms in the Río Tinto Mars analogue and report the absence of substantial negative effects on the immunoassay in the presence of 50?mM perchlorate (20 times higher than that found at the Phoenix landing site). Our SOLID instrument concept is an excellent option with which to detect biomolecules because it avoids the high-temperature treatments that may destroy organic matter in the presence of martian oxidants.     

A two-tiered approach to assessing the habitability of exoplanets

In the next few years, the number of catalogued exoplanets will be counted in the thousands. This will vastly expand the number of potentially habitable worlds and lead to a systematic assessment of their astrobiological potential. Here, we suggest a two-tiered classification scheme of exoplanet habitability. The first tier consists of an Earth Similarity Index (ESI), which allows worlds to be screened with regard to their similarity to Earth, the only known inhabited planet at this time. The ESI is based on data available or potentially available for most exoplanets such as mass, radius, and temperature. For the second tier of the classification scheme we propose a Planetary Habitability Index (PHI) based on the presence of a stable substrate, available energy, appropriate chemistry, and the potential for holding a liquid solvent. The PHI has been designed to minimize the biased search for life as we know it and to take into account life that might exist under more exotic conditions. As such, the PHI requires more detailed knowledge than is available for any exoplanet at this time. However, future missions such as the Terrestrial Planet Finder will collect this information and advance the PHI. Both indices are formulated in a way that enables their values to be updated as technology and our knowledge about habitable planets, moons, and life advances. Applying the proposed metrics to bodies within our Solar System for comparison reveals two planets in the Gliese 581 system, GJ 581 c and d, with an ESI comparable to that of Mars and a PHI between that of Europa and Enceladus.     

Estimation and classification of FM signals using time frequency transforms

This work deals with electronic warfare: we have to process an intercepted radar-emitted signal assumed to be frequency-modulated (FM). A battery of parametric models being available, we face the two main problems: the parameter estimation, given a model, and the classification, given the battery. For the former, we propose the classical maximum likelihood estimator (MLE) and an original numerical scheme to reach it. For the latter, we construct a test based on the estimated variances cross-ratios. Both perform very efficiently with respect to (w.r.t.) the Cramer-Rao lower bound (CRLB) and the rate of correct classification, respectively, for low signal-to-noise ratios (SNRs).