Physiological characterization of gravitaxis in Euglena gracilis and Astasia longa studied on sounding rocket flights.

Euglena gracilis is a photosynthetic, unicellular flagellate found in eutrophic freshwater habitats. The organisms control their vertical position in the water column using gravi- and phototaxis. Recent experiments demonstrated that negative gravitaxis cannot be explained by passive buoyancy but by an active physiological mechanism. During space experiments, the threshold of gravitaxis was determined to be between 0.08 and 0.12 x g. A strong correlation between the applied acceleration and the intracellular cAMP and Ca2+ was observed. The results support the hypothesis, that the cell body of Euglena, which is denser than the surrounding medium exerts a pressure onto the lower membrane and activates mechanosensitive Ca2+ channels. Changes in the membrane potential and the cAMP concentration are most likely subsequent elements in a signal transduction chain, which results in reorientation strokes of the flagellum.     

R3DE: Radiation Risk Radiometer-Dosimeter on the International Space Station--optical radiation data recorded during 18 months of EXPOSE-E exposure to open space

Radiation Risk Radiometer-Dosimeter E (R3DE) served as a device for measuring ionizing and non-ionizing radiation as well as cosmic radiation reaching biological samples located on the EXPOSE platform EXPOSE-E. The duration of the mission was almost 1.5 years (2008-2009). With four channels, R3DE detected the wavelength ranges of photosynthetically active radiation (PAR, 400-700?nm), UVA (315-400?nm), UVB (280-315?nm), and UVC (<280?nm). In addition, the temperature was recorded. Cosmic ionizing radiation was assessed with a 256-channel spectrometer dosimeter (see separate report in this issue). The light and UV sensors of the device were calibrated with spectral measurement data obtained by the Solar Radiation and Climate Experiment (SORCE) satellite as standard. The data were corrected with respect to the cosine error of the diodes. Measurement frequency was 0.1?Hz. Due to errors in data transmission or temporary termination of EXPOSE power, not all data could be acquired. Radiation was not constant during the mission. At regular intervals of about 2 months, low or almost no radiation was encountered. The radiation dose during the mission was 1823.98 MJ m(-2) for PAR, 269.03 MJ m(-2) for UVA, 45.73 MJ m(-2) for UVB, or 18.28 MJ m(-2) for UVC. Registered sunshine duration during the mission was about 152 days (about 27% of mission time).The surface of EXPOSE was most likely turned away from the Sun for considerably longer. R3DE played a crucial role on EXPOSE-EuTEF (EuTEF, European Technology Exposure Facility), because evaluation of the astrobiology experiments depended on reliability of the data collected by the device. Observed effects in the samples were weighted by radiation doses measured by R3DE.     

Identification of morphological biosignatures in Martian analogue field specimens using in situ planetary instrumentation

We have investigated how morphological biosignatures (i.e., features related to life) might be identified with an array of viable instruments within the framework of robotic planetary surface operations at Mars. This is the first time such an integrated lab-based study has been conducted that incorporates space-qualified instrumentation designed for combined in situ imaging, analysis, and geotechnics (sampling). Specimens were selected on the basis of feature morphology, scale, and analogy to Mars rocks. Two types of morphological criteria were considered: potential signatures of extinct life (fossilized microbial filaments) and of extant life (crypto-chasmoendolithic microorganisms). The materials originated from a variety of topical martian analogue localities on Earth, including impact craters, high-latitude deserts, and hydrothermal deposits. Our in situ payload included a stereo camera, microscope, M?ssbauer spectrometer, and sampling device (all space-qualified units from Beagle 2), and an array of commercial instruments, including a multi-spectral imager, an X-ray spectrometer (calibrated to the Beagle 2 instrument), a micro-Raman spectrometer, and a bespoke (custom-designed) X-ray diffractometer. All experiments were conducted within the engineering constraints of in situ operations to generate realistic data and address the practical challenges of measurement. Our results demonstrate the importance of an integrated approach for this type of work. Each technique made a proportionate contribution to the overall effectiveness of our "pseudopayload" for biogenic assessment of samples yet highlighted a number of limitations of current space instrument technology for in situ astrobiology.     

Gravitaxis and graviperception in flagellates.

There is strong evidence that gravitactic orientation in flagellates and ciliates is mediated by an active physiological gravireceptor rather than by passive alignment of the cells in the water column. In flagellates the threshold for graviorientation was found to be at 0.12 x g on a slow rotating centrifuge during the IML-2 mission on the Shuttle Columbia and a subsequent parabolic rocket flight (TEXUS). During the IML-2 mission no adaptation to microgravity was observed over the duration of the space flight, while gravitaxis was lost in a terrestrial closed environmental system over the period of almost two years. Sedimenting statoliths are not likely to be involved in graviperception because of the small size of the cells and their rotation around the longitudinal axis during forward locomotion. Instead the whole cytoplasmic content of the cell, being heavier than the surrounding aqueous medium (1.05 g/ml), exerts a pressure on the lower membrane. This force activates stretch-sensitive calcium specific ion channels which can be inhibited by the addition of gadolinium which therefore abolishes gravitaxis. The channels seem to mainly allow calcium ions to pass since gravitaxis is blocked by the addition of the calcium ionophore A23187 and by vanadate which blocks the Ca-ATPase in the cytoplasmic membrane. Recently, a gene for a mechanosensitive channel, originally sequenced for Saccharomyces, was identified in Euglena by PCR. The increase in intracellular free calcium during reorientation can be visualized by the fluorophore Calcium Crimson using laser excitation and image intensification. This result was confirmed during recent parabolic flights. The gated calcium changes the membrane potential across the membrane which may be the trigger for the reorientation of the flagellum. cAMP plays a role as a secondary messenger. Photosynthetic flagellates are suitable candidates for life support systems since they absorb CO2 and produce oxygen. Preliminary experiments are discussed.     

The Rosetta Mission: Flying Towards the Origin of the Solar System

The ROSETTA Mission, the Planetary Cornerstone Mission in the European Space Agency’s long-term programme Horizon 2000, will rendezvous in 2014 with comet 67P/Churyumov-Gerasimenko close to its aphelion and will study the physical and chemical properties of the nucleus, the evolution of the coma during the comet’s approach to the Sun, and the development of the interaction region of the solar wind and the comet, for more than one year until it reaches perihelion. In addition to the investigations performed by the scientific instruments on board the orbiter, the ROSETTA lander PHILAE will be deployed onto the surface of the nucleus. On its way to comet 67P/Churyumov-Gerasimenko, ROSETTA will fly by and study the two asteroids 2867 Steins and 21 Lutetia.     

ROMAP: Rosetta Magnetometer and Plasma Monitor

The scientific objectives, design and capabilities of the Rosetta Lander’s ROMAP instrument are presented. ROMAP’s main scientific goals are longterm magnetic field and plasma measurements of the surface of Comet 67P/Churyumov-Gerasimenko in order to study cometary activity as a function of heliocentric distance, and measurements during the Lander’s descent to investigate the structure of the comet’s remanent magnetisation. The ROMAP fluxgate magnetometer, electrostatic analyser and Faraday cup measure the magnetic field from 0 to 32 Hz, ions of up to 8000 keV and electrons of up to 4200 keV. Additional two types of pressure sensors – Penning and Minipirani – cover a pressure range from 10⁻⁸ to 10¹ mbar. ROMAP’s sensors and electronics are highly integrated, as required by a combined field/plasma instrument with less than 1 W power consumption and 1 kg mass.     

The putative mechanical strength of comet surface material applied to landing on a comet

The comet lander PHILAE (part of the ESA mission ROSETTA) is going to touch down on comet 67P/Churyumov–Gerasimenko in 2014. Landing dynamics depend on the mechanical strength of the surface material: in an extremely soft material, the lander (100 kg, 1 m/s touch-down velocity) may sink in too deep for successful operation while on a very hard surface the probability for bouncing and overturning increases. It is shown that direct knowledge on the strength of cometary surface material is very limited. In our view, even the Deep Impact experiment could not provide a reliable value of the mechanical strength of comet Tempel 1. We discuss the definition of “strength” and revise the ideas on cometary surface strength and theories that describe the low-velocity (≈1 m/s) impact of blunt bodies into dust-rich, fluffy cometary materials. Available direct and indirect measurements and data are critically reviewed. Lessons learnt from laboratory measurements to verify our equations of motion are presented as well. Conclusions for Philae are drawn: most likely, the soft landing will lead to a typical penetration of the lander's feet of up to 20 cm.     

Time profile of cosmic radiation exposure during the EXPOSE-E mission: the R3DE instrument

The aim of this paper is to present the time profile of cosmic radiation exposure obtained by the Radiation Risk Radiometer-Dosimeter during the EXPOSE-E mission in the European Technology Exposure Facility on the International Space Station's Columbus module. Another aim is to make the obtained results available to other EXPOSE-E teams for use in their data analysis. Radiation Risk Radiometer-Dosimeter is a low-mass and small-dimension automatic device that measures solar radiation in four channels and cosmic ionizing radiation as well. The main results of the present study include the following: (1) three different radiation sources were detected and quantified-galactic cosmic rays (GCR), energetic protons from the South Atlantic Anomaly (SAA) region of the inner radiation belt, and energetic electrons from the outer radiation belt (ORB); (2) the highest daily averaged absorbed dose rate of 426 μGy d(-1) came from SAA protons; (3) GCR delivered a much smaller daily absorbed dose rate of 91.1 μGy d(-1), and the ORB source delivered only 8.6 μGy d(-1). The analysis of the UV and temperature data is a subject of another article (Schuster et al., 2012 ).     

Cometary ion observations at and within the cometopause-region of comet Halley

Three distinct boundaries are identified from the PICCA cometary ion observations within the innermost part of the coma of comet Halley: (1) the 'cometopause' at a cometocentric distance R_c 1.5×10⁵ km, characterized by the appearance of water-group ions well above background; (2) the 'cold cometary plasma boundary' at R_c 3×10⁴ km, characterized by a sudden and simultaneous decrease in the temperatures of all cometary ions, and (3) the 'ionopause' at R_c 6000 km, characterized by a fast decrease in the intensity of all cometary ions by a factor 3–5. Between the first two boundaries only ions with masses less than 50 amu are present, showing distinct maximum intensities at 18, 32 and 44 amu at the second boundary. Downstream of the second boundary also ions of mass 12, 64, 76, 86 and 100 amu are detected.