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21.
In cells irradiation by heavy ions has been hypothesized to produce microlesions, regions of local damage. In cell membranes this damage is thought to manifest itself in the form of holes. The primary evidence for microlesions comes from morphological studies of cell membranes, but this evidence is still controversial, especially since holes also have been observed in membranes of normal, nonirradiated, cells. However, it is possible that damage not associated with histologically discernable disruptions may still occur. In order to resolve this issue, we developed a system for detecting microlesions based on liposomes filled with fluorescent dye. We hypothesized that if microlesions form in these liposomes as the result of irradiation, then the entrapped dye will leak out into the surrounding medium in a measurable way. Polypropylene vials containing suspensions of vesicles composed of either dipalmitoyl phosphatidylcholine, or a combination of egg phosphatidylcholine and cholesterol were irradiated at the Brookhaven National Laboratory using 56Fe ions at 1 GeV/amu. In several cases we obtained a significant loss of the entrapped dye above the background level. Our results suggest that holes may form in liposomes as the result of heavy ion irradiation, and that these holes are large enough to allow leakage of cell internal contents that are at least as large as a 1 nm diameter calcein molecule.  相似文献   
22.
Because activities in space necessarily involve chronic exposure to a heterogeneous charged particle radiation field it is important to assess the influence of dose-rate and the possible modulating role of heavy particle fragmentation on biological systems. Using the well-studied cataract model, mice were exposed to plateau 600 MeV/amu 56Fe ions either as acute or fractionated exposures at total doses of 5 - 504 cGy. Additional groups of mice received 20, 360 and 504 cGy behind 50 mm of polyethylene, which simulates body shielding. The reference radiation consisted of 60Co gamma radiation. The animals were examined by slit lamp biomicroscopy over their three year life spans. In accordance with our previous observations with heavy particles, the cataractogenic potential of the 600 MeV/amu 56Fe ions was greater than for low-LET radiation and increased with decreasing dose relative to gamma-rays. Fractionation of a given dose of 56Fe ions did not reduce the cataractogenicity of the radiation compared to the acute regimen. Fragmentation of the beam in the polyethylene did not alter the cataractotoxicity of the ions, either when administered singly or in fractions.  相似文献   
23.
With the mathematical basis for the precise analysis of developmental processes in plants, the patterns of growth in phototropic and gravitropic responses have become better understood. A detailed temporal and spatial quantification of a growth process is an important tool for evaluating hypotheses about the underlying physiological mechanisms. Studies of growth rates and curvature show that the original Cholodny-Went hypothesis cannot explain the complex growth patterns during tropic responses of shoots and roots. In addition, regulating factors other than the lateral redistribution of hormones must be taken into account. Electrophysiological studies on roots led to a modification of the Cholodny-Went hypothesis in that redistributions of bioelectrical activities are observed.  相似文献   
24.
A relatively simple method is presented which eliminates previously reported (Oct. 1985) erratic estimation performance associated with Cartesian formulations of the extended Kalman filter (EKF) for the 2D angle-only emitter location problem. The technique is based on an initialization procedure which combines a priori probability density function (pdf) information with single measurement a posteriori pdf information in a manner which is more efficient than the EKF. Simulation results are presented which demonstrate the utility of the technique as compared with a previously offered modified gain EKF  相似文献   
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26.
Demands for portability have fueled significant developments in new battery technology. These developments have resulted in many more options in selecting the battery type for use in a particular project, but since most applications today are opting for rechargeable battery systems, the availability of battery charging solutions can become an equally important criteria in the selection process. Complicating this process are the demands for fast-but safe-charging with charge algorithms easily implemented with low-cost hardware. With the higher levels of complexity attendant with these more demanding algorithms, solutions have come primarily from the integrated circuit industry and the purpose of this paper is to provide a few examples of the latest efforts in this arena, specifically as addressed to lead-acid, nickel metal-hydride, and lithium-ion technologies  相似文献   
27.
Using data from the Wide Field Camera EUV all-sky survey, we have established upper limits to the EUV flux from a sample of 30 bright, nearby, non-active spiral galaxies. These galaxies were chosen to be those most likely to be detected in the EUV on the basis of (i) low interstellar absorption within our own galaxy, (ii) brightness in other wavebands, (iii) high star formation activity, and (iv) proximity. The derived EUV upper limits are restrictive, and establish for the first time that the EUV flux escaping from galaxies does not constitute a major component of their bolometric luminosity, and in particular that it cannot be the sink for the energy injected into the interstellar medium by supernova explosions, as had been suggested following the failure to detect this power in the X-ray band.  相似文献   
28.
Eagle-Picher Energy Products (EPEP) has been manufacturing and testing large lithium ion cells (up to 100-Ah) for several years. Recently, work has focused on the testing of different chemistries at variable temperatures and designing and fabricating 100-Ah cylindrical cells. For the aircraft application the largest concern is irreversible capacity loss at elevated temperatures (70°C). In contrast, for the aerospace application shelf-life and cycle life are critical. EPEP has found that the major contributor to the loss in low temperature performance due to high temperature testing, was the positive electrode. EPEP discuss recent results of variable temperature cycling and 100-Ah cell performance  相似文献   
29.
Experiments under varied gravitational accelerations as well as in density-adjusted media showed that sensation of gravity in protists may be linked to the known principles of mechanosensation. Paramecium, a ciliate with clear graviresponses (gravitaxis and gravikinesis) is an ideal model system to prove this hypothesis since the ciliary activity and thus the swimming behaviour is controlled by the membrane potential. It has also been assumed that the cytoplasmic mass causes a distinct stimulation of the bipolarly distributed mechano-sensitive K+ and Ca2+ ion channels in the plasma membrane in dependence of the spatial orientation of the cell. In order to prove this hypothesis, different channel blockers are currently under investigation. Gadolinium did not inhibit gravitaxis in Paramecium, showing that it does not specifically block gravireceptors. Further studies concentrated on the question of whether second messengers are involved in the gravity signal transduction chain. Exposure to 5 g for up to 10 min led to a significant increase in cAMP.  相似文献   
30.
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