Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

典型加筋板的优化设计

对飞机结构设计中典型加筋板优化设计问题进行了研究 ,对典型加筋板形式、优化问题、计算方法和优化策略进行了描述 ,编写了优化设计程序 ,并进行了算例考核。计算结果表明 ,优化设计结果合理 ,可对该类加筋板的结构设计提供参考和指导。     

U型复合材料结构件热压罐固化变形及补偿技术应用研究

以复合材料U型构件为研究对象,采用数值模拟的方法对U型件进行热压罐温度场的模拟,模拟出在不同时间段工装和工件的温度分布情况,模拟工件在工装传热和变形影响下的内部传热、固化度、最终变形情况和残余应力水平等。根据模拟结果,优化热压罐固化工艺参数,从而改善固化热变形问题,根据分析结果制定补偿方案并实施验证,同时优化工装结构形式,采用工装型面补偿技术对零件固化变形进行预补偿。结果表明,补偿后零件变形后的外形与理论几何外形相差1.37mm,验证了补偿技术的可行性。工装型面补偿技术可以有效提高复合材料结构件的制造精度。     

月面巡视探测器动力学建模

动力学模型是研究星球探测车运动的基础。以某月面巡视探测器原理样机为背景,建立了其动力学模型,并进行了数值仿真分析。在说明月壤机械特性的基础上,结合地面车辆力学的知识,分析了松散月壤上刚性车轮的受力,考虑了主动驱动和被动驱动的情形,利用数学拟合的方法得到了满足工程要求的简化模型。在一定假设条件下,以牛顿欧拉法建立了三自由度整车动力学模型,建模时同时考虑了驱动和转向的相互影响。最后,数值仿真结果定性地验证了月面巡视探测器动力学模型的正确性。     

卫星大型挠性天线弹性振动抑制的研究

论文从工程实践角度探讨了具有大尺寸（直径几米～几十米）和高精度（０．０５°）的挠性天线指向控制问题。在借鉴和改进有关抑制挠性天线弹性振动研究的基础上，初步设计了星—地回路的挠性天线指向控制方案。最后，通过分析数值仿真结果，给出了提高挠性天线指向精度的主要因素。     

Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

偏置动量系统中的挠性天线指向控制研究

从工程实践角度,探讨了在目前广泛采用的偏置动量轮三轴姿态稳定卫星上,进行大尺寸（３～５ｍ）和高指向精度（０．０５°）的挠性天性指向控制问题。在建立具有挠性天线的星体动力学模型,以及在借鉴和改进有关抑制挠性天线振动研究的基础上,设计了三轴稳定卫星平台上的挠性天线指向控制系统的方案。最后,根据数值仿真结果,给出了影响挠性天线指向精度的主要因素。     

红外地球敏感器地面静电放电模拟试验方法研究

 设计了红外地球敏感器的地面模拟卫星静电放电试验方法。对卫星的空间环境以及结构特点的分析结果表明,地球同步静止轨道卫星表面存在静电放电的可能性。通过静电放电试验找到了红外地球敏感器易受干扰的部位并提出了解决措施。     

Rubbing-Induced Vibration Response Analysis of Dual-Rotor-Casing System

Considering gyroscopic effects caused by rotational speed,torsional vibration as well as coupling effects among inner rotor,out rotor and casing,a dynamic model of the dual-rotor-casing system is established using finite element(FE)method.By comparing the natural characteristics obtained from MATLAB and ANSYS,the developed model is verified.Then rubbing-induced vibration responses in dual-rotor-casing system are analyzed.The effects of rotational speed and speed ratio on rubbing vibration responses of the system are discussed.Results show that different combined frequency components will appear in the spectrum except two unbalanced excitation frequencies and their multiple frequency components,and these frequencies can be used as the dual-rotor aero-engine rubbing failure diagnosis frequencies when rubbing occurs.Besides,the amplitude of torsional vibration is larger than that of lateral vibration under the same working condition,and speed ratio has a great impact on the periodicity of the system rubbing-induced motion trajectory.The amplitude of rubbing-induced responses under counter-rotation is less than that under co-rotation with the same parameters.     

镍对C/SiC化学气相渗透碳的催化研究

用液相浸渗还原法将Ni渗入C/SiC复合材料,研究复合材料中Ni对化学气相渗透PyC过程的影响.用重量分析法研究Ni的催化作用,用扫描电子显微镜(SEM)、X射线衍射(XRD)技术分析材料的微观结构及组成.结果表明,Ni对化学气相渗透PyC具有催化作用,它加速了PyC的沉积,Ni含量为4.4%时增重率达最大值9.98%.沉积的PyC为细小颗粒聚集体.CVI过程中Ni与基体SiC反应生成Ni2Si,Ni3Si.