Genomic instability and tumorigenic induction in immortalized human bronchial epithelial cells by heavy ions

Carcinogenesis is postulated to be a progressive multistage process characterized by an increase in genomic instability and clonal selection with each mutational event endowing a selective growth advantage. Genomic instability as manifested by the amplification of specific gene fragments is common among tumor and transformed cells. In the present study, immortalized human bronchial (BEP2D) cells were irradiated with graded doses of either 1GeV/nucleon ⁵⁶Fe ions or 150 keV/μm alpha particles. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Tumorigenic cells showed neither ras mutations nor deletion in the p16 tumor suppressor gene. In contrast, they harbored mutations in the p53 gene and over-expressed cyclin D1. Genomic instability among transformed cells at various stage of the carcinogenic process was examined based on frequencies of PALA resistance. Incidence of genomic instability was highest among established tumor cell lines relative to transformed, non-tumorigenic and control cell lines. Treatment of BEP2D cells with a 4 mM dose of the aminothiol WR-1065 significantly reduced their neoplastic transforming response to ⁵⁶Fe particles. This model provides an opportunity to study the cellular and molecular mechanisms involved in malignant transformation of human epithelial cells by heavy ions.     

Neoplastic transformation of hamster embryo cells by heavy ions

We have studied the induction of morphological transformation of Syrian hamster embryo cells by low doses of heavy ions with different linear energy transfer (LET), ranging from 13 to 400 keV/μm. Exponentially growing cells were irradiated with ¹²C or ²⁸Si ion beams generated by the Heavy Ion Medical Accelerator in Chiba (HIMAC), inoculated to culture dishes, and transformed colonies were identified when the cells were densely stacked and showed a crisscross pattern. Over the LET range examined, the frequency of transformation induced by the heavy ions increased sharply at very low doses no greater than 5 cGy. The relative biological effectiveness (RBE) of the heavy ions relative to 250 kVp X-rays showed an initial increase with LET, reaching a maximum value of about 7 at 100 keV/μm, and then decreased with the further increase in LET. Thus, we confirmed that high LET heavy ions are significantly more effective than X-rays for the induction of in vitro cell transformation.     

Neoplastic cell transformation by energetic heavy ions and its modification with chemical agents.

For many years we have been interested in understanding the potential carcinogenic effects of cosmic rays. We have studied the oncogenic effects of cosmic rays with accelerator-produced heavy particle radiation and with a cultured mammalian cell system--C3H10T1/2 cells. Our quantitative data obtained with carbon, neon, silicon, and iron particles showed that RBE is both dose and LET dependent for neoplastic cell transformation. RBE is higher at lower dose, and RBE increases with LET up to about 200 keV/micrometer. In nonproliferation confluent cells, heavy-ion induced transformation damage may not be repairable, although a dose modifying factor of about 1.7 was observed for X-ray radiation. Our recent studies with super-heavy high-energy particles, e.g., 960 MeV/U U235 ions (LET = 1900 keV/micrometer), indicate that these ions with a high inactivation cross-section can cause neoplastic cell transformation. The induction of cell transformation by radiation can be modified with various chemicals. We have found that the presence of DMSO (either during or many days after irradiation) decreased the transformation frequency significantly. It is, therefore, potentially possible to reduce the oncogenic effect of cosmic rays in space through some chemical protection.     

Diurnal variations of ion flux intensity on a synchronous orbit

The data from the synchronous-orbit satellites of the Gorizont series are used to study the dependences of the ion flux variation amplitudes in the synchronous altitude region (the diurnal behaviour) on particle energies and on the form and rigidity of the particle energy spectrum. The proton fluxes were measured in the energy range E 60–120 keV, and the [N,0]²⁺ and [C,N,0]⁴⁺ ion fluxes in the energy range E 60–70 keV/e.

The ratio of the diurnal variation amplitudes of the studied ions is shown to correspond to the similarity of their energy spectra in the E/Q representation. The magnetically-quiet time gradient of the distribution function F(μ,J,L) in the synchronous-orbit region is shown to be (∂F/∂L)=0 for the H⁺ and [N,0]²⁺ ions and (∂F/∂L) > 0 for the [C,N,0]⁴⁺ ions (at the values of μ corresponding to the examined energy ranges). During magnetically-disturbed periods the inner boundary of the (∂F/∂L)=0 region shifts to lower L and (∂ F/∂L) = O in the synchronous altitude region must be also for the [C,N,O]⁴⁺ ions.     

Oncogenic transformation of mammalian cells by ultrasoft X-rays and alpha particles.

For a better understanding of oncogenic cell transformation by ionizing radiation, we conducted experiments with ultrasoft X rays and low energy alpha particles. Confluent C3H10T1/2 cells were irradiated by Al-K (1.5 keV) X rays or alpha particles from plutonium through a thin mylar sheet, on which the cells attached and grew. Our results indicated that Al-K X rays were more effective in causing cell inactivation and oncogenic transformation than 60Co gamma rays but less effective than 1.0 and 3.7 MeV alpha particles. There was no significant difference between 1.0 and 3.7 MeV alpha particles in transforming cells although the latter were slightly more effective than the former in producing lethal effect. These results indicated that track structure is important in causing biological effects by ionizing radiation.     

Near infrared observations of comet Halley from Vega 2

Spatial distribution of the continuum radiation in the range of 0.95–1.9 μm presumes total dust production rate of the comet of 10ρ tonne s⁻¹ (ρ is the dust material density) and its angular distribution proportional cos . Observations of the water vapor band at 1.38 μ m reveal strong jets, their time shift from the dust jet measured in situ is consistent with gas velocity of 0.82±0.1 km s⁻¹ and dust velocity of 0.55±0.08 km s⁻¹. The OH vibrational-rotational bands observed are excided directly via photolysis of water vapor. Water vapor production rate deduced from the H₂O band and OH band intensities is 8×10²⁹ s⁻¹. Intensity of the CN(0,0) band result in the CN column density of 9×10¹² cm⁻², i.e. larger by a factor of 3 than given by the violet band.     

Interaction of artificially injected plasma flows with ionospheric plasma

Results of rocket experiments on study of plasma flows (PF) artificially injected by sources separated from vehicles and their effect on medium parameters in ionosphere at altitudes 160:230 km are presented.PF were injected comprising lithium ions with velocities 1,2 x 10⁴ m/sec. and cesium-potassium ions with velocities (1,4–1,5)x10³ m/sec. Mass flow rate in case of lithium PS is 2 mg/sec, and in case of cesium-potassium PS is 0,2 g/sec. During experiments mass-spectrometer measurements of ion medium content in ranges of different ion masses were held, disturbancies of electric fields with frequencies up to 20 kHz and electron flows with energies 0,7keV, 4,6keV and over 40 keV were controlled at distancies from 150m to (500–600)m between plasma source and scientific equipment.     

Residual chromatin breaks as biodosimetry for cell killing by carbon ions

We have studied the relationship between cell killing and the induction of residual chromatin breaks on various human cell lines and primary cultured cells obtained by biopsy from patients irradiated with either X-rays or heavy-ion beams to identify potential bio-marker of radiosensitivity for radiation-induced cell killing. The carbon-ion beams were accelerated with the Heavy Ion Medical Accelerator in Chiba (HIMAC). Six primary cultures obtained by biopsy from 6 patients with carcinoma of the cervix were irradiated with two different mono-LET beams (LET= 13 keV/μm, 76 keV/μm) and 200kV X rays. Residual chromatin breaks were measured by counting the number of non-rejoining chromatin fragments detected by the premature chromosome condensation (PCC) technique after a 24 hour postirradiation incubation period. The induction rate of residual chromatin breaks per cell per Gy was the highest for 76 keV/μm beams on all of the cells. Our results indicated that cell which was more sensitive to the cell killing was similarly more susceptible to induction of residual chromatin breaks. Furthermore there is a good correlation between these two end points in various cell lines and primary cultured cells. This suggests that the detection of residual chromatin breaks by the PCC technique may be useful as a predictive assay of tumor response to cancer radiotherapy.     

LaBr3(Ce)元件破损在线监测关键核素确定及效率校准

常用的元件破损在线监测方法受外在因素影响较大，以γ能谱中关键裂变产物核素比活度进行在线定量分析是一种可靠的元件破损监测方法。针对新型LaBr₃(Ce)元件破损监测仪，设计了元件破损模拟实验，对逸出的裂变产物采用HPGe和LaBr₃(Ce)探测器进行了对比测量。通过不同冷却时间γ能谱的核素分析，确定了¹³⁵Xe，⁸⁸Kr，¹³⁸Xe，⁸⁸Rb，¹³⁸Cs等可作为LaBr₃(Ce)元件破损γ能谱监测的关键核素。同时，制备了覆盖能量范围(250～2 400)keV，含⁶⁰Co，¹³⁷Cs，¹³³Ba；²⁴¹Am，¹⁵²Eu；¹⁰⁹Cd，⁸⁸Y，⁵⁷Co及²⁴Na的4组放射性标准溶液，在建立的效率校准系统上，校准了LaBr₃(Ce)在线监测仪的效率。在反应堆一次异常情况分析中，已校准的LaBr₃(Ce)元件破损监测仪对关键核素的现场分析结果与HPGe的取样分析结果一致，表明效率校准结果准确，校准方法可靠。     

LaBr3(Ce)元件破损在线监测关键核素确定及效率校准

常用的元件破损在线监测方法受外在因素影响较大，以γ能谱中关键裂变产物核素比活度进行在线定量分析是一种可靠的元件破损监测方法。针对新型LaBr₃(Ce)元件破损监测仪，设计了元件破损模拟实验，对逸出的裂变产物采用HPGe和LaBr₃(Ce)探测器进行了对比测量。通过不同冷却时间γ能谱的核素分析，确定了¹³⁵Xe，⁸⁸Kr，¹³⁸Xe，⁸⁸Rb，¹³⁸Cs等可作为LaBr₃(Ce)元件破损γ能谱监测的关键核素。同时，制备了覆盖能量范围(250～2 400)keV，含⁶⁰Co，¹³⁷Cs，¹³³Ba；²⁴¹Am，¹⁵²Eu；¹⁰⁹Cd，⁸⁸Y，⁵⁷Co及²⁴Na的4组放射性标准溶液，在建立的效率校准系统上，校准了LaBr₃(Ce)在线监测仪的效率。在反应堆一次异常情况分析中，已校准的LaBr₃(Ce)元件破损监测仪对关键核素的现场分析结果与HPGe的取样分析结果一致，表明效率校准结果准确，校准方法可靠。     

Interplanetary dust interaction with comets: Production of x-rays

Recent results of theoretical investigations related to generation of high-energy (0.1-1 keV) photons in comets due to production of high-temperature (3×10⁵-10⁷ K) plasma clots from collisions of cometary and interplanetary grains at high relative velocities (70-700 km s⁻¹ at heliocentric distances R=0.01-1 AU) are summarized and main features of the process are marked.     

Diagnosing the storm-time ring current and other magnetospheric plasma domains using relativistic electron data

Ring current ions and relativistic electrons simultaneously measured on board MOLNIYA-1 are analyzed in comparison with the ground-based magnetometer data for the period of a strong magnetic storm (|D_st|max≈230 nT). Injection of >500 keV electrons into the slot region (L≈3) near equatorial plane is occurred on time scale ≈1 hour, when, during the magnetic storm maximum, the extreme low-latitude position of auroral electrojets is reached and ring current becomes more symmetrical. Positions of both the ring current maximum and electron intensity maximum (L_max) are consistent to our previous result: |D_st|_max = 2.75 • 10⁴/L⁴_max. An extreme storm-time low-latitude position of the west electrojet center (for amplitudes of |D_st|_max up to 600 nT) is shown to be in a good consistence with this empirical dependence. It is supposed the trapped radiation boundary collapses down to L≈L_max in the course of the storm main phase.     

Numerical simulations of the radiance from the comet 46P/Wirtanen in the various configurations of the measurements during “Rosetta” mission

The work we present deals with the spectrometric measurements of VIRTIS instrument of the Comet P/Wirtanen planned for the Rosetta mission. This spectrometer can monitor (VIRTIS M channel: 0.250μm – 0.980μm; Δκ=20cm⁻¹; 0.980 – 5.0 μm; Δκ = 5cm⁻¹; VIRTIS H channel: 2.0 μm – 5.0 μm; Δκ=2cm⁻¹) the nucleus and the coma in order to provide a general picture of coma's composition, the production of gas and dust, the relationship of coma production to surface composition and the structure and variation of mineralogy of the nucleus surface. During the mission the observation conditions of the spectroscopic investigation change due to different relative positions spacecraft/comet, and to the different illumination conditions of the surface at various distances of the comet to the Sun. The nucleus surface is continuously modified by the ice sublimation accompanied by gas and dust emission. Consequently the surface also its spectrophotometric properties changes and their monitoring can give a new insight. The important role of simulations is to predict the results of measurements in various experimental condition what, in the future, can help in interpretation of the measured data.

In this paper the first results of our simulation the radiance from the comet in the 0.25–5.0μm spectral range at two distances from the Sun (1AU and 3AU) are shown. The distance between the Rosetta orbiter and the nucleus surface as well as the sun zenith angles are taken into account according to the Rosetta mission phases. In fact the surface and coma properties vary along the comet orbit, and should be taken into account in our calculations. The optical parameters of the dust on the surface (e.g. reflectance) and in the coma (e.g. Q_ext) were calculated from optical constants of possible comet analogues. The thermodynamic parameters of the comet are taken from the models of comet evolution. Through this kind of modelling it is possible to identify the surface characteristics in spectra of the radiation from the surface of nucleus transmitted through the coma loaded with dust and gases.

Even if the “Rosetta mission” is postponed, with the consequence of a target change, we think that our idea and the method used for the simulations can be useful also for the new Rosetta target - the comet 67P/Churyumov Gerasimenko.     

ROSAT observations of the Chamaeleon star forming cloud

Two soft X-ray images of the Chamaeleon I star forming cloud obtained with the ROSAT Position Sensitive Proportional Counter are presented. Seventy reliable, and perhaps 19 additional, X-ray sources are found. Up to Ninety percent of these sources are certainly or probably identified with T Tauri stars formed in the cloud. Twenty to 35 are probably previously unrecognized ‘weak’ T Tauri (WTT) stars. T Tauri X-ray luminosities range from log , or 10² – 10⁴ times solar levels, with mean in the 0.2–2.5 keV band. The X-ray luminosities of well-studied Chamaeleon cloud members are correlated with a complex of four stellar properties: effective temperature, mass, radius and bolometric luminosity. The spatial distribution, H-R diagram locations of the stars indicate WTT and CTT are coeval. The total premain sequence population of the cloud is likely to be > 100 stars, with WTT stars outnumbering ‘classical’ T Tauri (CTT) stars by 2:1.     

Intensity profiles of the multiple scattering light near the cometary nucleus

The multiple scattering of solar radiation in the cometary atmosphere is treated with the method of successive scattering. Referring to in situ measurements of comet Halley about the size and spatial distributions of dust, the optical thickness τ₁ of dust has been estimated, i.e. τ₁=0.03 at wavelength λ=0.62μm in a quiet time, but τ₁=0.3 when the outbursts/jets occur. In the derivation of τ₁, optical properties of dust including a mixing ratio of absorbing to silicate grains, are determined based on the polarimetry of P/Halley at λ=0.62μm observed during the phase angles over Nov. 1985 to May 1986 at the Dodaira Station of Tokyo Astronomical Observatory.

It is found that a temporary enhancement of τ₁ leads an increase of the upward reflected intensity when the surface albedo A of the nucleus is less than 0.04, but the reverse is true when A>0.04. On the other hand, the intensity of the downward radiation at the surface of the nucleus always decreases as an increase of τ₁.     

Energy content of accelerated electrons and ions in intense solar flares

The energy content of nonthermal particles in solar flares is shared between accelerated electrons and ions. It isimportant for understanding the particle acceleration mechanism in solar flares. Yohkoh observed a few intense flares which produced both strong gamma-ray lines and electron bremsstrahlung continuum. We analyze energy spectra of X-class solar flares on October 27, 1991(X6.1), November 6, 1997 (X9.4), July 14, 2000 (X5.7) and November 24, 2000 (X2.3). The accelerated electron and proton spectra are derived from a spectral analysis of their high-energy photon emission and the energy contents in >1 MeV electrons and >10 MeV protons are estimated to be 6×l0²⁸ – 4×10³⁰ and 2×10²⁸ – 5×10²⁹ erg, respectively. We study the flare to flare variation in the energy content of >1 MeV electrons and >10 MeV protons for the four Yohkoh gamma-ray flares. Ratios of >1 MeV electron energy content to >10 MeV proton energy content are roughly within an order of magnitude.     

Present results of the joint radiobiological ESA/IBMP experiments “seeds” aboard Cosmos 2044 and 2229 - correlation of micro-dosimetric data and damage endpoints in Arabidopsis thaliana plants

Experimental data obtained from two Cosmos missions (2044, 2229) performed with dry plant seeds of Arabidopsis thaliana in a Biostack configuration are compared. Biological stress and genetic risk are induced in dry seeds by cosmic radiation, especially the high energetic, densely ionizing component of eavy ons (HI). Subdivision of impacting HI particles into LET-groups (15–35; 35–100; >100 keV/μm) showed the contribution of each LET group to the induction of different biological damage endpoints (survival, cell transformation, lethal plant development). An attempt is presented to comprehend the influence of the spatial energy deposition as a biophysical HI track parameter.     

X-ray spectroscopy and variability of AGN detected in the 2 Ms Chandra Deep Field-North Survey

We investigate the nature of the faint X-ray source population through X-ray spectroscopy and variability analyses of 136 active galactic nuclei (AGN) detected in the 2 Ms Chandra Deep Field-North Survey with >200 background-subtracted 0.5–8.0 keV counts [F_{0.5–8.0 keV} = (1.4−200) × 10⁻¹⁵ erg cm⁻² s⁻¹]. Our preliminary spectral analyses yield median spectral parameters of Γ = 1.61 and intrinsic N_H = 6.2 × 10²¹ cm⁻² (z = 1 assumed when no redshift available) when the AGN spectra are fitted with a simple absorbed power-law model. However, considerable spectral complexity is apparent (e.g., reflection, partial covering) and must be taken into account to model the data accurately. Moreover, the choice of spectral model (i.e., free vs. fixed photon index) has a pronounced effect on the derived JVH distribution and, to a lesser extent, the X-ray luminosity distribution. We also find that among the 136 AGN, 10 (≈7%) show significant Fe K emission-line features with equivalent widths in the range 0.1–1.3 keV. Two of these emission-line AGN could potentially be Compton thick (i.e., Γ < 1.0 and large Fe K equivalent width). Finally, we find that 81 (≈60%) of the 136 AGN show signs of variability, and that this fraction increases significantly (≈80–90%) when better photon statistics are available.     

ROSAT PSPC observations of two starburst galaxies

We present results from ROSAT observations of NGC 1808 and NGC 2903. Exposures of 10 ksec each with the PSPC detector show X-ray sources at the central positions of both galaxies which are classified as nuclear starburst galaxies. Both targets, NGC 1808 and NGC 2903 appear slightly extended in X-ray maps in the energy band 0.1–2.4 keV. The X-ray spectrum of NGC 1808 shows almost complete absorption below 0.5 keV, indicating an extremely high hydrogen column density towards that source (N_H ≈ 8 × 10²¹cm⁻² resulting from model fits on the PSPC spectrum). In case of NGC 2903, the number of counts in the ROSAT band is significantly lower than expected from a previous EINSTEIN investigation of the source.     

A Far Infrared Photometer (FIRP) for the Infrared Telescope in Space (IRTS)

We describe the design and calibration of the Far-Infrared Photometer (FIRP), one of four focal plane instruments on the Infrared Telescope in Space (IRTS). The FIRP will provide absolute photometry in four bands centered at 150, 250, 400, and 700 μm with spectral resolution λ/Δλ ≈ 3 and spatial resolution ΔΘ = 0.5 degrees. High sensitivity is achieved by using bolometric detectors operated at 300 mK in an AC bridge circuit. The closed-cycle ³He refrigerator can be recycled in orbit. A 2 K shutter provides a zero reference for each field of view. More than 10% of the sky will be surveyed during the ≈3 week mission lifetime with a sensitivity of <10⁻¹³ W·cm⁻²·sr⁻¹ per 0.5 degree pixel.