Calcium signaling in plant cells in altered gravity.

Changes in the intracellular Ca2+ concentration in altered gravity (microgravity and clinostating) evidence that Ca2+ signaling can play a fundamental role in biological effects of microgravity. Calcium as a second messenger is known to play a crucial role in stimulus-response coupling for many plant cellular signaling pathways. Its messenger functions are realized by transient changes in the cytosolic ion concentration induced by a variety of internal and external stimuli such as light, hormones, temperature, anoxia, salinity, and gravity. Although the first data on the changes in the calcium balance in plant cells under the influence of altered gravity have appeared in 80th, a review highlighting the performed research and the possible significance of such Ca2+ changes in the structural and metabolic rearrangements of plant cells in altered gravity is still lacking. In this paper, an attempt was made to summarize the available experimental results and to consider some hypotheses in this field of research. It is proposed to distinguish between cell gravisensing and cell graviperception; the former is related to cell structure and metabolism stability in the gravitational field and their changes in microgravity (cells not specialized to gravity perception), the latter is related to active use of a gravitational stimulus by cells presumebly specialized to gravity perception for realization of normal space orientation, growth, and vital activity (gravitropism, gravitaxis) in plants. The main experimental data concerning both redistribution of free Ca2+ ions in plant cell organelles and the cell wall, and an increase in the intracellular Ca2+ concentration under the influence of altered gravity are presented. Based on the gravitational decompensation hypothesis, the consequence of events occurring in gravisensing cells not specialized to gravity perception under altered gravity are considered in the following order: changes in the cytoplasmic membrane surface tension --> alterations in the physicochemical properties of the membrane --> changes in membrane permeability, --> ion transport, membrane-bound enzyme activity, etc. --> metabolism rearrangements --> physiological responses. An analysis of data available on biological effects of altered gravity at the cellular level allows one to conclude that microgravity environment appears to affect cytoskeleton, carbohydrate and lipid metabolism, cell wall biogenesis via changes in enzyme activity and protein expression, with involvement of regulatory Ca2+ messenger system. Changes in Ca2+ influx/efflux and possible pathways of Ca2+ signaling in plant cell biochemical regulation in altered gravity are discussed.     

Possible actions of gravity on the cellular machinery.

Since the first flight of the ESA Biorack on the German Spacelab Mission D1 in 1985 evidence has been obtained that biological cells and small unicellular organisms function differently under conditions of microgravity. However, there is still lack of scientific proof that these effects are caused by a direct influence on the cells in the weightlessness condition. The question how normal gravity may play a role in cellular activity is being addressed and the results show that gravity may provide important signals during certain state transitions in the cell. These would be gravity-sensitive windows in the biological process. Also, by amplification mechanisms inside the cell, the cell may assume a state that is typical for normal gravity conditions and would change in microgravity. Experimental tools are discussed that would provide the conditions to obtain evidence for direct action of gravity and for the possible existence of gravity-sensitive windows.     

Effect of gravity field on the nonequilibrium/nonlinear chemical oscillation reactions.

Biological systems have evolved for a long time under the normal gravity. The Belousov-Zhabotinsky (BZ) reaction is a nonlinear chemical system far from the equilibrium that may be considered as a simplified chemical model of the biological systems so as to study the effect of gravity. The reaction solution is comprised of bromate in sulfuric acid as an oxidizing agent, 1,4-cyclohexanedione as an organic substrate, and ferroin as a metal catalyst. Chemical waves in the BZ reaction-diffusion system are visualized as blue and red patterns of ferriin and ferroin, respectively. After an improvement to the tubular reaction vessels in the experimental setup, the traveling velocity of chemical waves in aqueous solutions was measured in time series under normal gravity, microgravity, hyper-gravity, and normal gravity using the free-fall facility of JAMIC (Japan Microgravity Center), Hokkaido, Japan. Chemical patterns were collected as image data via CCD camera and analyzed by the software of NIH image after digitization. The estimated traveling velocity increased with increasing gravity as expected. It was clear experimentally that the traveling velocity of target patterns in reaction diffusion system was influenced by the effect of convection and correlated closely with the gravity field.     

Use of reduce gravity conditions for the measurements of forces acting against the displacement of liquids inside containers

A setup called Controlled Rotation of Liquids (CROL) has been developed and used to investigate rotational behavior of liquids interacting with solids under reduced gravity conditions. The CROL setup allows us to evaluate the acceleration needed to overcome the interfacial forces that hinder liquid displacement inside different size containers. The well known Wilhelmy equation is used to explain the experimental results. Comparison between experimental results and theoretical predictions are presented and discussed.     

失重飞机搭载气 /液两相流实验研究

利用俄罗斯IL-76MDK失重飞机对失重条件下方形截面(12mm×12mm)管道内气/液两相流动进行了实验研究,观测到失重条件下气/液两相泡状流、弹状流和环状流等三种主要流型,同时得到了气、液两相介质的流量、温度和实验段内压力等结果,分析了失重条件下方形截面管道内气/液两相流型转换的条件.      

Magnetic levitation-based Martian and Lunar gravity simulator.

Missions to Mars will subject living specimens to a range of low gravity environments. Deleterious biological effects of prolonged exposure to Martian gravity (0.38 g), Lunar gravity (0.17 g), and microgravity are expected, but the mechanisms involved and potential for remedies are unknown. We are proposing the development of a facility that provides a simulated Martian and Lunar gravity environment for experiments on biological systems in a well controlled laboratory setting. The magnetic adjustable gravity simulator will employ intense, inhomogeneous magnetic fields to exert magnetic body forces on a specimen that oppose the body force of gravity. By adjusting the magnetic field, it is possible to continuously adjust the total body force acting on a specimen. The simulator system considered consists of a superconducting solenoid with a room temperature bore sufficiently large to accommodate small whole organisms, cell cultures, and gravity sensitive bio-molecular solutions. It will have good optical access so that the organisms can be viewed in situ. This facility will be valuable for experimental observations and public demonstrations of systems in simulated reduced gravity.     

An improved altimeter-derived gravity anomaly from shipborne gravity based on the mean sea surface height constraint factors method

Coastal marine gravity modeling faces challenges due to the degradation of the quality and poor coverage of altimeter data in coastal regions. The effective fusion of shipborne gravity data and altimeter-derived marine gravity data can make shipborne gravity data more useful for the accurate estimation of altimeter-derived coastal marine gravity. A mean sea surface height constraint factor (MSSHCF) method based on the ordinary kriging method and the remove-restore technique is proposed to fuse altimeter-derived gravity model with shipborne gravity data. In this method, all data are standardized during the interpolation process to reduce the error and mean sea surface as a vertical variable is added to the semi-variance function in ordinary kriging to obtain the residual shipborne gravity as corrected data source. The coastal marine gravity models V2.1 and V3.1 which fused altimeter-derived gravity data with shipborne gravity data and V1.1 without shipborne gravity data at a spatial resolution of 1′×1′ can be obtained. Validation experiments show that the accuracy of the gravity model V3.1 obtained by the MSSHCF method more closely agrees with the validated gravity model DTU17 and SS V31 than the model V2.1 obtained by the ordinary kriging interpolation method and the V1.1 model. Our results were validated against shipborne gravity data; the accuracy of model V3.1 was 4.95 % higher than the model V1.1 in South China Sea area A and 2.48 % higher in South China Sea area B. Meanwhile, the accuracy of model V3.1 was 2.07 % higher than model V2.1 in South China Sea area A and 2.42 % higher in South China Sea area B. The effects of distance from the coast and sea depth on the marine gravity model were also evaluated. The results show that the gravity model V3.1 has higher accuracy with the change in ocean distance and depth than the V2.1 and V1.1 gravity models. Thus, our study shows that the MSSHCF method effectively refines coastal altimeter-derived gravity using shipborne gravity data.     

Skeletogenesis in sea urchin larvae under modified gravity conditions.

From many points of view, skeletogenesis in sea urchins has been well described. Based on this scientific background and considering practical aspects of sea urchin development (i.e. availability of material, size of larvae, etc.), we wanted to know whether orderly skeletogenesis requires the presence of gravity. The objective has been approached by three experiments successfully performed under genuine microgravity conditions (in the STS-65 IML-2 mission of 1994; in the Photon-10 IBIS mission of 1995 and in the STS-76 S/MM-03 mission of 1996). Larvae of the sea urchin Sphaerechinus granularis were allowed to develop in microgravity conditions for several days from blastula stage onwards (onset of skeletogenesis). At the end of the missions, the recovered skeletal structures were studied with respect to their mineral composition, architecture and size. Live larvae were also recovered for post-flight culture. The results obtained clearly show that the process of mineralisation is independent of gravity: that is, the skeletogenic cells differentiate correctly in microgravity. However, abnormal skeleton architectures were encountered, particularly in the IML-2 mission, indicating that the process of positioning of the skeletogenic cells may be affected, directly or indirectly, by environmental factors, including gravity. Larvae exposed to microgravity from blastula to prism/early pluteus stage for about 2 weeks (IBIS mission), developed on the ground over the next 2 months into normal metamorphosing individuals.     

Modeling gravity effects on water retention and gas transport characteristics in plant growth substrates

Growing plants to facilitate life in outer space, for example on the International Space Station (ISS) or at planned deep-space human outposts on the Moon or Mars, has received much attention with regard to NASA’s advanced life support system research. With the objective of in situ resource utilization to conserve energy and to limit transport costs, native materials mined on Moon or Mars are of primary interest for plant growth media in a future outpost, while terrestrial porous substrates with optimal growth media characteristics will be useful for onboard plant growth during space missions. Due to limited experimental opportunities and prohibitive costs, liquid and gas behavior in porous substrates under reduced gravity conditions has been less studied and hence remains poorly understood. Based on ground-based measurements, this study examined water retention, oxygen diffusivity and air permeability characteristics of six plant growth substrates for potential applications in space, including two terrestrial analogs for lunar and Martian soils and four particulate substrates widely used in reduced gravity experiments. To simulate reduced gravity water characteristics, the predictions for ground-based measurements (1 − g) were scaled to two reduced gravity conditions, Martian gravity (0.38 − g) and lunar gravity (0.16 − g), following the observations in previous reduced gravity studies. We described the observed gas diffusivity with a recently developed model combined with a new approach that estimates the gas percolation threshold based on the pore size distribution. The model successfully captured measured data for all investigated media and demonstrated the implications of the poorly-understood shift in gas percolation threshold with improved gas percolation in reduced gravity. Finally, using a substrate-structure parameter related to the gaseous phase, we adequately described the air permeability under reduced gravity conditions.     

The influence of gravity on the process of development of animal systems

The development of animal systems is described in terms of a series of overlapping phases: pattern specification; differentiation; growth; and aging. The extent to which altered (micro) gravity (g) affects those phases is briefly reviewed for several animal systems. As a model, amphibian egg/early embryo is described. Recent data derived from clinostat protocols indicates that microgravity simulation alters early pattern specification (dorsal/ventral polarity) but does not adversely influence subsequent morphogenesis. Possible explanations for the absence of catastrophic microgravity effects on amphibian embryogenesis are discussed.     

Cytoskeleton and gravity at work in the establishment of dorso-ventral polarity in the egg of Xenopus laevis

The establishment of polarities during early embryogenesis is essential for normal development. Amphibian eggs are appropriate models for studies on embryonic pattern formation. The animal-vegetal axis of the axially symmetrical amphibian egg originates during oogenesis and foreshadows the main body axis of the embryo. The dorso-ventral polarity is epigenetically established before first cleavage. Recent experiments strongly suggest that in the monospermic eggs of the anuran Xenopus laevis both the cytoskeleton and gravity act in the determination of the dorso-ventral polarity. In order to test the role of gravity in this process, eggs will be fertilized under microgravity conditions during the SL-D1 flight in 1985. In a fully automatic experiment container eggs will be kept under well-defined conditions and artificially fertilized as soon as microgravity is reached; eggs and embryos at different stages will then be fixed for later examination. Back on earth the material will be analysed and we will know whether fertilization under microgravity conditions is possible. If so, the relation of the dorso-ventral axis to the former sperm entry point will be determined on the whole embryos; in addition eggs and embryos will be analysed cytologically.     

微重力下胶原蛋白纤维化及羟基磷灰石结晶的初步研究

在长期空间飞行过程中, 骨质丢失是一个严重问题. 羟基磷灰石(HAP)晶体是骨骼的主要成分, 骨骼中的胶原蛋白纤维在HAP生长结晶过程中起到关键作用. 研究了胶原蛋白纤维化过程在模拟微重力和常重力条件下的变化, 对以胶原 蛋白纤维作为模板生长出的HAP晶体形貌进行了观察. 结果表明, 不同浓度胶原蛋白溶液中形成的胶原蛋白纤维, 其内部孔隙数量和尺寸在模拟微重力条件下要明显大于常重力条件下, 胶原蛋白纤维内部孔隙的分布也不同于常重力条 件下的结果. 以模拟微重力条件下形成的胶原蛋白纤维为模板生长出的HAP 晶体主要为立方体状, 而以常重力条件下形成的胶原蛋白纤维为模板生长出的 HAP晶体形貌主要为板状. 该结果有助于未来进一步阐明空间骨质丢失的机理.      

A simulation of Martian gravity field recovery by using a near equatorial orbiter

An improvement to the Martian gravity field may be achieved by means of future orbiting spacecraft with small eccentricity and low altitude exemplified through a newly proposed mission design that may be tested in upcoming reconnaissance of Mars. Here, the near equatorial orbital character (with an inclination approximating 10°, eccentricity as 0.01 and semi-major axis as 4000 km) is considered, and its contribution to Martian gravity field solution is analyzed by comparing it with a hypothetical polar circular orbiter. The solution models are evaluated in terms of the following viewpoints: power spectra of gravity field coefficients, correlations of low degree zonal coefficients, precise orbit determination, and error distribution of both Mars free air gravity anomaly and areoid. At the same time, the contributions of the near equatorial orbiters in low degree zonal coefficients time variations are also considered. The present results show that the near equatorial orbiter allows us to improve the accuracy of the Martian gravity field solution, decrease correlation of low degree zonal coefficients, retrieve much better time variable information of low degree zonal coefficients, improve precise orbit determination, and provide more accurate Mars free air gravity anomaly and areoid around the equatorial region.     

The effect of gravity on plant germination.

An axis clinostat was constructed to create micro and negative gravity also a rotated flat disk was constructed with different rotation rates to give increased gravity, by centrifugal force up to 48 g. Rice seeds were grown on agar in tubes at the constant air temperature of 20 degrees C under an average light condition of 110 micromol/m2/sec(PPF). Humidity was not controlled but was maintained above 90%. Since the tube containers were not large enough for long cultivation, shoot and root growth were observed every 12 hours until the sixth day from seeding. The lengths of shoots and roots for each individual plant were measured on the last day. The stem lengths were increased by microgravity but the root lengths were not. Under the negative gravity, negative orthogeotropism and under microgravity, diageotropism was observed. No significant effect of increased gravity was observed on shoot and root growth.     

DMFC内部气液两相流动与电性能的短时落塔实验研究

利用国家微重力实验室落塔提供的短时微重力实验环境,对常重力和微重力条件下直接甲醇燃料电池(DMFC)内部的气液两相流动形态和相应电性能等的影响进行了实验研究,发现在微重力条件下,DMFC阳极流道内CO₂气泡速度很小,气泡尺寸随着时间的推移而不断长大,甚至堵塞流道;流道堵塞现象随电流增大而急剧强化.电性能曲线显示,在浓差极化区存在显著的重力效应,电性能的恶化随浓差极化程度的加强而增大.      

Lunar gravity field modeling critical analysis and challenges

This paper summarizes and provides a critical analysis of the historical developments of lunar gravitational models from the earliest use of ground based tracking systems of the Lunar Orbiter to the Lunar Prospector mission. This encompasses a comprehensive and critical analysis of the various methods used in the estimation of the gravity coefficients and the processing of large batches of diverse measurements and data types. It has been shown that weakness exists in the current models of the lunar gravity field, which is primarily due to the lack of far side lunar tracking data information, which makes the lunar potential modeling difficult but expected to be overcome as data from SELENE satellite-to-satellite tracking becomes available. Comparisons of various lunar models reveal an agreement in the low order coefficients of the spherical harmonics. However, substantial differences in the models exist in the higher-order harmonics. A numerical comparison has been presented showing the performance of all the contemporary lunar gravitational models used within the astrodynamics community and available in public domain. Improvements to the current models are part of a continuing process and the recent model improvements and future possibilities in lunar gravity modeling are discussed. A brief review of the recent missions has been presented. It is hoped that this critical review will benefit the researchers by presenting the historical as well as state of the art in this field.     

地面模拟静止微重力环境中导线先期着火特性研究

针对在微重力环境中运行的载人航天飞行器上的电缆和导线在工作时由于电流过载导致温度升高而引起着火的情况,提出了"功能模拟"实验原理,并且利用地面实验设备对微重力环境下导线的着火前期特性进行了功能模拟实验研究.通过实验,得到了在微重力情况下由于浮升力的减小使自然对流减弱导致电流过载时导线的热平衡温度高于地面正常重力情况,从而证明了这正是引起航天飞行器着火的潜在点火源.      

Interaction of growth-determining systems with gravity

The experiments have been carried out with lettuce shoots on board the Salyut-7 orbital station, the Kosmos-1667 biological satellite and under ground conditions at 180° plant inversion. By means of the centrifuge Biogravistat-1M the threshold value of gravitational sensitivity of lettuce shoots has been determined on board the Salyut-7 station. It was found to be equal to 2.9 × 10⁻³g for hypocotyls and 1.5 × 10⁻⁴g for roots. The following results have been received in the experiment performed on board the Kosmos-1667 satellite: a) under microgravity the proliferation of the meristem cells and the growth of roots did not differ from the control; b) the growth of hypocotyls in length was significantly enhanced in microgravity; c) under microgravity transverse growth of hypocotyls (increase in cross sectional area) was significantly increased due to enhancement of cortical parenchyma cell growth. At 180° inversion in Earth's gravity root extension growth and rate of cell division in the root apical meristem were decreased. The determination of DNA-fuchsin value in the nuclei of the cell root apexes showed that inversion affected processess of the cell cycle preceeding cytokinesis.     

微重力煤燃烧研究现状及发展趋势

微重力环境抑制了煤在地面燃烧时所受的浮力影响,提供了发现煤燃烧新现象及获得更准确的煤燃烧数据的理想条件,相关研究有助于揭示煤燃烧的基本规律和浮力对煤燃烧的作用,研究结果可用于校核和发展煤燃烧模型,进而促进地面煤的高效低污染利用.综述了国内外微重力煤燃烧研究现状,介绍了最新进展和成果,并对未来研究进行展望.      

Microgravity effects on neural retina regeneration in the newt.

Data on forelimb and eye lens regeneration in urodeles under spaceflight conditions (SFC) have been obtained in our previous studies. Today, evidence is available that SFC stimulate regeneration in experimental animals rather than inhibit it. The results of control on-ground experiments with simulated microgravity suggest that the stimulatory effect of SFC is due largely to weightlessness. An original experimental model is proposed, which is convenient for comprehensively analyzing neural regeneration under SFC. The initial results described here concern regeneration of neural retina in Pleurodeles waltl newts exposed to microgravity simulated in radial clinostat. After clinorotation for seven days (until postoperation day 16), a positive effect of altered gravity on structural restoration of detached neural retina was confirmed by a number of criteria. Specifically, an increased number of Mullerian glial cells, an increased relative volume of the plexiform layers, reduced cell death, advanced redifferentiation of retinal pigment epithelium, and extended areas of neural retina reattachment were detected in experimental newts. Moreover, cell proliferation in the inner nuclear layer of neural retina increased as compared with control. Thus, low gravity appears to intensify natural cytological and molecular mechanisms of neural retina regeneration in lower vertebrates.