The simulated silicification of bacteria--new clues to the modes and timing of bacterial preservation and implications for the search for extraterrestrial microfossils

Evidence of microbial life on Earth has been found in siliceous rock formations throughout the geological and fossil record. To understand the mechanisms of silicification and thus improve our search patterns for evidence of fossil microbial life in rocks, a series of controlled laboratory experiments were designed to simulate the silicification of microorganisms. The bacterial strains Pseudomonas fluorescens and Desulphovibrio indonensis were exposed to silicifying media. The experiments were designed to determine how exposure time to silicifying solutions and to silicifying solutions of different Si concentration affect the fossilization of microbial biofilms. The silicified biofilms were analyzed using transmission electron microscopy (TEM) in combination with energy-dispersive spectroscopy. Both bacterial species showed evidence of silicification after 24 h in 1,000 ppm silica solution, although D. indonensis was less prone to silicification. The degree of silicification of individual cells of the same sample varied, though such variations decreased with increasing exposure time. High Si concentration resulted in better preservation of cellular detail; the Si concentration was more important than the duration in Si solution. Even though no evidence of amorphous silica precipitation was observed, bacterial cells became permineralized. High-resolution TEM analysis revealed nanometer-sized crystallites characterized by lattice fringe-spacings that match the (10-11) d-spacing of quartz formed within bacterial cell walls after 1 week in 5,000 ppm silica solution. The mechanisms of silicification under controlled laboratory conditions and the implication for silicification in natural environments are discussed, along with the relevance of our findings in the search for early life on Earth and extraterrestrial life.     

Gypsum-permineralized microfossils and their relevance to the search for life on Mars

Abstract Orbital and in situ analyses establish that aerially extensive deposits of evaporitic sulfates, including gypsum, are present on the surface of Mars. Although comparable gypsiferous sediments on Earth have been largely ignored by paleontologists, we here report the finding of diverse fossil microscopic organisms permineralized in bottom-nucleated gypsums of seven deposits: two from the Permian (～260?Ma) of New Mexico, USA; one from the Miocene (～6?Ma) of Italy; and four from Recent lacustrine and saltern deposits of Australia, Mexico, and Peru. In addition to presenting the first report of the widespread occurrence of microscopic fossils in bottom-nucleated primary gypsum, we show the striking morphological similarity of the majority of the benthic filamentous fossils of these units to the microorganisms of a modern sulfuretum biocoenose. Based on such similarity, in morphology as well as habitat, these findings suggest that anaerobic sulfur-metabolizing microbial assemblages have changed relatively little over hundreds of millions of years. Their discovery as fossilized components of the seven gypsiferous units reported suggests that primary bottom-nucleated gypsum represents a promising target in the search for evidence of past life on Mars. Key Words: Confocal laser scanning microscopy-Gypsum fossils-Mars sample return missions-Raman spectroscopy-Sample Analysis at Mars (SAM) instrument-Sulfuretum. Astrobiology 12, 619-633.     

Microbial influences on local carbon isotopic ratios and their preservation in carbonate

Analysis of the carbon isotopic composition of carbonates is a valuable tool for studying microbial processes and looking for evidence of life. Microbial fixation of CO2 and conversion of organic carbon to CO2 can produce measurable delta 13C shifts in a microbial mat environment. Diffusion modeling demonstrates that substantial isotopic shifts can develop within the mat and in the diffusion boundary layer in the fluid when CO2 fixation is rapid and prolonged for several hours. Carbonates that precipitate during rapid CO2 fixation can preserve these microbially produced isotopic shifts. However, continued precipitation during intervals when respiration dominates or after the cessation of active microbial growth commonly dilutes autotrophic isotopic signatures. Thus, preserved isotopic signatures rarely reflect the magnitude of isotopic shifts within the mat. Interpretation of observed isotopic shifts in microbial mat carbonate depends on fully characterizing ambient delta 13C and eliminating other origins for isotopic shifts. The carbon isotopic composition of reservoirs can vary substantially, both on Earth and on other planets. Characterizing the reservoir composition and any changes through time is critical to evaluating microbially induced shifts. In addition, careful evaluation of non-microbial causes for shifts in isotopic composition is essential for a reliable interpretation. Complicating processes include recrystallization, calcite precipitation over extended periods of time, variable precipitation rates and water chemistry, and mixing of carbonates having different isotopic signatures.     

Planetary preservation: the need for legal provision

The Earth has been hit by NEOs many times in its history and there is always a risk that this will happen again. While we are beginning to acquire the technology that could warn of and divert a future collision, the legal instruments needed to facilitate a global response to the problem are lacking, especially given that the most likely means of diverting a NEO would involve nuclear weapons that could also be used on Earth. An analysis of the current legal situation is used to support the argument that new rules must be created. The author also discusses US Air Force policy on the subject and whether planetary defense is consistent with its goal of achieving and sustaining US superiority in space.     

A transmission electron microscopy study of silica and kerogen biosignatures in approximately 1.9 Ga gunflint microfossils

Microfossils preserved in chert from the;1.9 Ga Gunflint Formation (Schreiber Beach, Ontario, Canada) were studied with transmission electron microscopy (TEM) and analytical TEM (ATEM). Our goals were to uncover the style of silicification relative to the distribution of organic matter, and to evaluate the distribution and evolution of organic matter, at submicroscopic spatial scales. Petrographically the microfossils typically display filamentous or coccoidal morphologies, and consist of quartz crystals surrounded by kerogen along grain boundaries. ATEM analysis revealed that quartz associated with kerogen consists of 200-500nm-sized, round crystallites, whereas the chert matrix is comprised of randomly oriented, polygonal microquartz (5-10 microm). Silica spheroids found within some fossils consist of quartz subgrains in an amorphous to poorly crystalline matrix, suggesting that precipitation of opaline silica on organic matter occurred with subsequent but incomplete transformation to quartz. Some coccoidal microfossils surround large euhedral quartz crystals (up to 5 microm in diameter) that appeared to have influenced the distribution of kerogen during crystal growth. These euhedral quartz crystals commonly contain elongated (50-100 nm) iron-rich crystallites. Energy-loss, near-edge structure analysis of kerogen associated with a coccoidal microfossil showed that it is composed of amorphous carbon with no evidence of graphitization. TEM results revealed significant differences in the style of silicification between microbe-shaped microfossils and their surrounding chert matrix, as well as the presence of amorphous kerogen.     

Flexibility in system design and implications for aerospace systems

The purpose of this paper is to review the concept of flexibility as discussed in various fields of investigations, to extract its characteristic features, and to explore its implications in the case of aerospace system design. In order to discuss any subject matter clearly, it is necessary to begin with a clear set of definitions. Indeed much can be gained through careful and consistent definitions of terms alone. Flexibility however is a word rich with ambiguity. While it is being increasingly used in various fields, few attempts have been made to formally define, quantify, and propose ways for achieving flexibility. This paper proposes to fill in part of this gap by synthesizing a clear and consistent definition of flexibility. It will do so by reviewing the usage of the term in various fields of inquiries, and show that it is indeed possible to clearly and unambiguously characterize flexibility, and to disentangle it from closely related concepts.     

Satellite communications and the Internet: implications for the outer space treaty

The speedily expanding Internet is in the process of transforming the technological, economic, and policy bases for nation-state regulation of telecommunications, including space-based satellite networks. Deployment of the packet-switched Internet has accelerated the liberalization of telecommunications markets and has led to far-reaching regulatory restructuring and policy shifts regarding state ownership and control of networks and information flows. As space-based GMPCS networks become integral parts of the globalizing Internet infrastructure, the state-centric legal paradigm requiring state “authorization and continuing supervision” of space activities by “non-governmental entities” stipulated under Article VI of the OST and associated treaties forming the outer space legal regime will be called increasingly into question. This paper examines the technological, economic/trade, and security issues that question whether the existing state-centric paradigm for regulating Internel-based GMPCS satellite systems will remain in legal phase with emerging liberalized regulatory regimes for terrestrial Internet-based infractructures.     

Serpentinization and its implications for life on the early Earth and Mars

Ophiolites, sections of ocean crust tectonically displaced onto land, offer significant potential to support chemolithoautotrophic life through the provision of energy and reducing power during aqueous alteration of their highly reduced mineralogies. There is substantial chemical disequilibrium between the primary olivine and pyroxene mineralogy of these ophiolites and the fluids circulating through them. This disequilibrium represents a potential source of chemical energy that could sustain life. Moreover, E (h)-pH conditions resulting from rock- water interactions in ultrabasic rocks are conducive to important abiotic processes antecedent to the origin of life. Serpentinization--the reaction of olivine- and pyroxene-rich rocks with water--produces magnetite, hydroxide, and serpentine minerals, and liberates molecular hydrogen, a source of energy and electrons that can be readily utilized by a broad array of chemosynthetic organisms. These systems are viewed as important analogs for potential early ecosystems on both Earth and Mars, where highly reducing mineralogy was likely widespread in an undifferentiated crust. Secondary phases precipitated during serpentinization have the capability to preserve organic or mineral biosignatures. We describe the petrology and mineral chemistry of an ophiolite-hosted cold spring in northern California and propose criteria to aid in the identification of serpentinizing terranes on Mars that have the potential to harbor chemosynthetic life.     

自动测试设备多开关模型的构造方法及其实现

通过对传统 TPS设计方法的分析 ,提出构造自动测试设备 (ATE)多开关模块模型的一种方法 ,从数学的角度将各类开关模块纳入统一的逻辑表述范畴以实现测试方案的灵活性和 TPS的通用性 ,并在此基础上探讨模型在ATE软件中的具体构建方法和实现过程。     

大气层外多拦截器协同跟踪与制导算法

针对大气层外多弹头多诱饵的进攻场景,采用多拦截器全拦截策略,提出了带故障诊断的协同跟踪算法、时间协同中制导律以及消除脱靶量的末制导律。首先,基于最小二乘算法以及误差传播理论,实现了局部信息融合以及测量方程的线性化,简化了非线性跟踪滤波算法的设计;并依托滤波算法,设计了传感器故障诊断算法,以排除其对跟踪效果的影响。然后,基于对拦截器和目标受力的合理简化,给出了相对运动解析解,设计了有限推力下的多拦截器时间协同中制导律以及末制导律,实现对多个目标的同时击毁。仿真结果显示,本文设计的协同跟踪与制导算法,可以有效估计目标的状态并排除故障传感器的干扰,实施对多个目标的时间协同拦截。     

Spaceflight effects on consecutive generations of peas grown onboard the Russian segment of the International Space Station

In the period from March 2003 to April 2005 we fulfilled five experimental cultivations of genetically marked dwarf pea species in greenhouse Lada installed in the Russian segment (RS) of the International Space Station (ISS). The purpose of this series of experiments was to make morphologic and genetic analysis of pea plants grown in successive generations.According to our results, pea growth and development over the full cycle of ontogenesis (from seed to seed) taking place in space greenhouse Lada were not different as compared with the ground control plants. In addition, four successive pea crops gathered in space flight did not loose their reproductive functions and formed viable seeds.Genetic analysis of the plants grown from the “space” and “ground” seeds produced by the first to fourth successive crops was performed using the methods of chromosomal aberrations count and Random Amplified Polymorphic DNA (molecular method). No genetic polymorphism was found either in the experimental or control crops. This can serve as a sound argument for the supposition that the genetic apparatus of plants is not impacted by exposure of several successive generations to the conditions of space flight.     

炭纤维壳体封头设计的几个问题

讨论了炭纤维壳体封头低压破坏的原因及3种破坏模式,即靠近赤道附近部位的封头发生断裂,极孔部位在环向应力作用下断裂和封头沿接头肩部外缘被剪(弯)断;得到了3种破坏模式的强度校核公式.给出了防止封头破坏宜采取的有效措施,对开孔很大的后封头,宜采取补强措施;给出了补强层厚度和补强范围的确定方法.     

Multiple Systems for Spatial Imagery: Transformations of Objects and Bodies

Problem-solving often requires imagining spatial changes. Object-based transformations allow imagining an object in a different orientation. Perspective transformations allow imagining changes in one's viewpoint. Three experiments tested the hypothesis that these two transformations are dissociable and specialized for different situations, by manipulating instructions and task parameters and measuring response times, errors, and introspective reports. Human experience with small objects such as telephones and clothes irons consists mostly of manipulation or observed manipulation, which is characterized by object-based transformations. Consistent with this experience, when participants made judgments about small manipulable objects, they showed a strong tendency to use object-based transformations. Experience with human bodies is more varied, including both object-like interactions and interactions in which one must estimate another's perspective. Accordingly, when making judgments about pictures of bodies, participants' selection of a spatial transformation depended on the type of judgment that needed to be made. When instructions violated these natural mappings, performance was impaired. These data argue for the view that multiple spatial transformation systems evolved to solve different spatial reasoning problems.     

Archaeal genome organization and stress responses: implications for the origin and evolution of cellular life

For DNA to be used as an informational molecule it must exist in the cell on the edge of stability because all genomic processes require local controlled melting. This presents mechanistic opportunities and problems for genomic DNA from hyperthermophilic organisms, whose unpackaged DNA could melt at optimal temperatures for growth. Hyperthermophiles are suggested to employ the novel positively supercoiling topoisomerase enzyme reverse gyrase (RG) to form positively supercoiled DNA that is intrinsically resistant to thermal denaturation. RG is presently the only archaeal gene that is uniquely found in hyperthermophiles and therefore is central to hypotheses suggesting a hypothermophilic origin of life. However, the suggestion that RG has evolved by the fusion of two pre-existing enzymes has led to hypotheses for a lower temperature for the origin of life. In addition to the action of topoisomerases, DNA packaging and the intracellular ionic environment can also manipulate DNA topology significantly. In the Euryarchaeota, nucleosomes containing minimal histones can adopt two alternate DNA topologies in a salt-dependent manner. From this we hypothesize that since internal salt concentrations are increased following an increase in temperature, the genomic effects of temperature fluctuations could also be accommodated by changes in nucleosome organization. In addition, stress-induced changes in the nucleoid proteins could also play a role in maintaining the genome in the optimal topological state in changing environments. The function of these systems could therefore be central to temperature adaptation and thus be implicated in origin of life scenarios involving hyperthermophiles.     

多层卫星网络的星间链路分析与路由协议

根据多层卫星星座网络的链路长度与可见性模型,建立了多层卫星网络的数学模型,并据此提出了一种适于多层卫星网络应用的层次路由协议。新协议主要包括拓扑测算、路由计算和路由转发。仿真计算结果表明,该协议的计算速度快、拓扑更新的通信开销小、链路利用率高,其性能优于Bellman和多层卫星网络路由(ML-SR)算法。     

分导飞行器多模型自适应控制方法研究

基于单位四元数描述和一种偏差动态线性化方法,得到了刚体飞行器的一种间接式多模型自适应控制律,解决了转动惯量不确定且可能发生突变的分导飞行器的姿态跟踪问题。该控制策略可以有效地检测出转动惯量的突变,然后切换到当前最合适的控制器进行控制。理论分析证明采用该控制策略闭环系统全局渐近稳定,数值仿真表明该方案具有比单模型自适应控制更好的暂态跟踪性能。
     

Oxidant enhancement in martian dust devils and storms: implications for life and habitability

We investigate a new mechanism for producing oxidants, especially hydrogen peroxide (H2O2), on Mars. Large-scale electrostatic fields generated by charged sand and dust in the martian dust devils and storms, as well as during normal saltation, can induce chemical changes near and above the surface of Mars. The most dramatic effect is found in the production of H2O2 whose atmospheric abundance in the "vapor" phase can exceed 200 times that produced by photochemistry alone. With large electric fields, H2O2 abundance gets large enough for condensation to occur, followed by precipitation out of the atmosphere. Large quantities of H2O2 would then be adsorbed into the regolith, either as solid H2O2 "dust" or as re-evaporated vapor if the solid does not survive as it diffuses from its production region close to the surface. We suggest that this H2O2, or another superoxide processed from it in the surface, may be responsible for scavenging organic material from Mars. The presence of H2O2 in the surface could also accelerate the loss of methane from the atmosphere, thus requiring a larger source for maintaining a steady-state abundance of methane on Mars. The surface oxidants, together with storm electric fields and the harmful ultraviolet radiation that readily passes through the thin martian atmosphere, are likely to render the surface of Mars inhospitable to life as we know it.     

碳粉燃料在火箭冲压发动机补燃室内燃烧特性的分析

采用概率密度函数(PDF)及确定轨道模型数值模拟一种环向进气的固体火箭冲压发动机补燃室气固两相流的掺混燃烧,考虑固体颗粒直径大小和补燃室长度对燃烧效率的影响。结果表明:增大固体颗粒直径,燃料的燃烧效率明显减小;增大补燃室长度,燃料的燃烧效率增大。     

Ultrastructural study of iron oxide precipitates: implications for the search for biosignatures in the Meridiani hematite concretions, Mars

Two terrestrial environments that have been proposed as analogs for the iron oxide precipitation in the Meridiani Planum region of Mars include the Rio Tinto precipitates and southern Utah marble concretions. Samples of two typical Utah iron oxide concretions and iron oxide precipitates in contact with biofilms from Rio Tinto have been studied to determine whether evidence could be found for biomediation in the precipitation process and to identify likely locations for fossil microorganisms. Scanning electron microscopy, energy dispersive X-ray, and gas chromatography-mass spectrometry (GC-MS) were used to search for biosignatures in the Utah marbles. The precipitation of iron oxides resembles known biosignatures, though organic compounds could not be confirmed with GC-MS analysis. In contrast, textural variations induced by biological activity are abundant in the modern Rio Tinto samples. Although no compelling evidence of direct or indirect biomediation was found in the Utah marbles, the ultrastructure of the iron oxide cement in the concretion suggests an inward growth during concretion precipitation from an initially spherical redox front. No indication for growth from a physical nucleus was found.     

C/C复合材料难熔金属二硅化物涂层防氧化机理研究

采用包埋技术在C/C复合材料表面制备了SiC-WSi2/MoSi2抗氧化涂层,通过恒温氧化试验以及TG-DTG、XRD和SEM等手段对该涂层的显微形貌、成分进行了分析,研究了涂层在1 500℃的抗氧化机理。结果表明,二硅化物的合金化及二硅化物与SiC的复合化提高了涂层的致密性、稳定性,而涂层表面长时间存在的致密的玻璃态SiO2,有效地阻止了氧气向涂层内部的扩散,使涂层表现出优异的高温防氧化能力。