Altered expression of mRNAs implicated in osteogenesis under conditions of simulated microgravity is regulated by CD200:CD200R

Mouse calvarial cells grown under simulated microgravity conditions (neutral buoyancy) show preferential differentiation towards the osteoclast lineage, as defined by surrogate mRNAs, bone nodule growth and TRAP⁺ cells, when compared with cells cultured under normal gravity conditions. This effect was suppressed in cultures which contained the immunoregulatory molecule CD200, and conversely enhanced by anti-CD200 mAb. Concomitant increases occur in expression of inflammatory cytokines, and their mRNAs, under simulated microgravity conditions. Again cultures containing exogenous CD200 showed suppressed cytokine and cytokine mRNA expression. Further alterations in osteoclastogenesis were seen using cells isolated from cytokine-receptor knockout mice. We conclude that, as assessed by altered expression of mRNAs associated with osteoblast differentiation, CD200:CD200R interactions play an important regulatory role in the enhanced osteoclastogenesis seen under simulated microgravity conditions, with changes in cytokine expression further modulating this effect.     

失重模拟技术的发展及其比较研究

失重或称微重力环境是载人航天轨道飞行中的重要环境因素,地面上失重模拟实验是航天前的重要准备工作之一。失重模拟方法及其设备多种多样,要依其目的不同而进行选择。文章对上述问题进行了充分的比较研究,并在此基础上指出了中性浮力水槽和失重飞机是失重模拟设备中最常用和最有效的工具。建议从我国的实际情况出发,适时的加以建造。     

Preliminary results of Texus 8 experiments on effects of surface tension minimum

A stable, observable liquid-gas interface was realized in an experimental cell (3 × 2 × 1 cm) under microgravity conditions. The liquid used was an aqueous 6.24 10^?3 molal solution of n-heptanol. A temperature gradient, established at the interface, resulted in the build-up of a Marangoni convection cell with the liquid flowing in an unusual direction (i.e. at the surface the liquid flowed from the cold to the hot side).The same experiment on Earth gives rise to a Marangoni cell superimposed on buoyancy cells. The Marangoni and the buoyancy cells then turn in opposite directions.     

表面张力贮箱设计中的中性浮力实验方法

介绍了中性浮力实验方法的原理和它在实验中所需的各种条件、设备以及实验用液体的选择。该实验方法能简便地模拟液体的微重力状态 ,并能给表面张力贮箱的设计提供很大的帮助。可利用它测量各种加速度下贮箱中推进剂的位置和形状 ,为表面张力贮箱推进剂管理装置(PMD)的设计提供可靠依据。     

The Impact of Variable Accelerations on Crystal Growth onboard Spacecraft by the Floating Zone Method

The numerical investigation of the impact of time-dependent accelerations (vibrations) on the flow and heat and mass transfer in the melt is carried out for the case of modeling the crystal growth by the floating zone method under conditions of microgravity that exist onboard spacecraft. The effects of the Archimedean buoyancy force and of vibrations of the free surface of fluid are considered separately. When solving the problem of the effect of the vibrations of the free surface of fluid, the previously obtained data were used. It is shown that vibrations of the free surface have a much stronger effect on the processes under consideration than the buoyancy. Some problems that are related to the newly discovered effects are discussed. The use of vibroprotected systems and a rotating magnetic field can help solve these problems. We plan to continue our investigations in future spacecraft experiments, in particular, at the International Space Station, which is under construction at the moment.     

Detection of the quantity of kinesin and microgravity-sensitive kinesin genes in rat bone marrow stromal cells grown in a simulated microgravity environment

Kinesin and kinesin-like proteins (KLPs) constitute a superfamily of microtubule motor proteins found in all eukaryotic organisms. Members of the kinesin superfamily are known to play important roles in many fundamental cellular and developmental processes. To date, few published studies have reported on the effects of microgravity on kinesin expression. In this paper, we describe the expression pattern and microgravity-sensitive genes of kinesin in rat bone marrow stromal cells cultured in a ground-based rotating bioreactor. The quantity of kinesin under the clinorotation condition was examined by immunoblot analysis with anti-kinesin. Furthermore, the distribution of kinesin at various times during clinorotation was determined by dual immunostaining, using anti-kinesin monoclonal antibody or anti-β-tubulin monoclonal antibody. In terms of kinesin quantity, we found that the ratios of the amounts of clinorotated/stationary KLPs decreased from clinorotation day 5 to day 10, although it increased on days 2 and 3. Immunofluorescence analysis revealed that kinesin in the nucleus was the first to be affected by simulated microgravity, following the kinesin at the periphery that was affected at various times during clinorotation. Real-time RT-PCR analysis of kinesin mRNA expression was performed and led to the identification of 3 microgravity-sensitive kinesin genes: KIF9, KIFC1, and KIF21A. Our results suggest that kinesin has a distinct expression pattern, and the identification of microgravity-sensitive kinesin genes offers insight into fundamental cell biology.     

Moments applied in the manual assembly of space structures: EASE biomechanics results from STS-61B

Measurements of the level and pattern of moments applied in the manual assembly of a space structure were made in extravehicular activity (EVA) and neutral buoyancy simulation (NBS). The Experimental Assembly of Structures in EVA program included the repeated assembly of a 3.6 m tetrahedral truss structure in EVA on STS-61B after extensive neutral buoyancy crew training. The flight and training structures were of equivalent mass and geometry to allow a direct correlation between EVA and NBS performance. A stereo photographic motion camera system was used to reconstruct in three dimensions rotational movements of structural beams during assembly. Moments applied in these manual handling tasks were calculated on the basis of the reconstructed movements taking into account effects of inertia, drag and virtual mass. Applied moments of 2.0 Nm were typical for beam rotations in EVA. Corresponding applied moments in NBS were typically up to five times greater. Moments were applied as impulses separated by several seconds of coasting in both EVA and NBS. Decelerating impulses were only infrequently observed in NBS.     

太阳电池对平流层飞艇热特性的影响分析

研究太阳电池对平流层飞艇驻空阶段热特性的影响。建立了太阳电池热模型、平流层飞艇热分析模型,包括热平衡方程、太阳直射辐射、天空散射辐射、地面反射辐射、大气长波辐射、地球长波辐射、蒙皮长波辐射、对流换热等;采用多节点模型,对平流层飞艇在驻空期间太阳电池、蒙皮与艇内氦气温度变化过程进行了数值模拟,得到了温度昼夜变化规律;分析了太阳电池（含隔热结构）的等效面积热阻、转换效率、铺装面积对平流层飞艇热特性的影响,得到了其温度昼夜变化规律。本文为平流层飞艇热性能分析和热控系统设计提供依据。     

超临界压力下航空煤油RP-3在水平细圆管内对流换热特性实验研究

为探究超临界压力下碳氢燃料在水平管内的对流换热规律,文章针对超临界条件下航空煤油RP-3在水平细圆管内的对流换热,分析了热流密度、进口雷诺数及浮升力对对流换热的影响。研究表明:沿流动方向,管内表面传热系数随热流密度的增大先减小后增大;在低进口温度及低进口雷诺数情况下,管内换热均出现先恶化后强化的现象,而随着进口温度和雷诺数的增加,此现象消失;浮升力对换热的影响随热流密度的增加而增加;浮升力对下表面换热的加强使得入口效应的影响在下表面先于上表面结束;受浮升力影响,上下壁最大温差可达50 K;质量流速的增加会抑制浮升力对换热的影响;准则数Grq/Grth可以很好地反映浮升力的变化趋势。以上研究结果可为采用碳氢燃料作冷却介质的各类飞行器主动热防护技术方案提供技术支撑。     

Effects of buoyancy on gas-jet diffusion flames: Experiment and theory

Theoretical and experimental research on the effects of buoyancy on gas-jet diffusion flames is described in this paper. Part of this research involves an assessment of existing data obtained under reduced-gravity conditions. The results show that uncertainties in our understanding of flame structure exist and further research is required before reliable predictions of ignition, stabilization and propagation of flames under microgravity conditions can be made. Steady-state and transient theories have been developed and used in the analysis of existing drop-tower data and new data obtained from a stationary experiment involving inverted flames. The result of this research has led to the definition of a microgravity experiment to be performed in space.     

On laboratory simulation and the effect of small temperature oscillations about the freezing point and ice formation on the evaporation rate of water on Mars

We report measurements of the evaporation rate of water under Mars-like conditions (CO2 atmosphere at 7 mbar and approximately 0 degrees C) in which small temperature oscillations about the freezing point repeatedly formed and removed a thin layer of ice. We found that the average evaporation at 2.7 +/- 0.5 degrees C without an ice layer (corrected for the difference in gravity on Earth and on Mars) was 1.24 +/- 0.12 mm/h, while at -2.1 +/- 0.3 degrees C with an ice layer the average evaporation rate was 0.84 +/- 0.08 mm/h. These values are in good agreement with those calculated for the evaporation of liquid water and ice when it is assumed that evaporation only depends on diffusion and buoyancy. Our findings suggest that such differences in evaporation rates are entirely due to the temperature difference and that the ice layer has little effect on evaporation rate. We infer that the formation of thin layers of ice on pools of water on Mars does not significantly increase the stability of water on the surface of Mars.     

Immobilization induces a very rapid increase in osteoclast activity

We studied in a randomized, strictly controlled cross-over design, the effects of 6 days 6 degrees head-down tilt bed rest (HDT) in eight male healthy subjects in our metabolic ward. The study consisted of two periods (phases) of 11 days each in order to allow for the test subjects being their own controls. Both study phases were identical with respect to environmental conditions, study protocol and diet. Two days before arriving in the metabolic ward the subjects started with a diet. The diet was continued in the metabolic ward. The metabolic ward period (1l days) was divided into three parts: 4 ambulatory days, 6 days either HDT or control and 1 recovery day. Continuous urine collection started on the first day in the metabolic ward to analyze calcium excretion and bone resorption markers. On the 2nd ambulatory day in the metabolic ward and on the 5th day in HDT or control blood was drawn to analyze serum calcium, parathyroid hormone, and bone formation markers. Urinary calcium excretion was, as early as the first day in immobilization, increased (p<0.01). CTX- and NTX-excretion stayed unchanged in the first 24 h in HDT compared to the control. But already on the 2nd day of immobilization, both bone resorption markers significantly increased. We conclude from these results--pronounced rise of bone resorption markers--that already 24 h of immobilization induce a significant rise in osteoclast activity in healthy subjects. Thus, it appears possible to use short-term bed rest studies as a first step for the development of countermeasures to immobilization.     

Response and adaptation of bone cells to simulated microgravity

Bone loss induced by microgravity during space flight is one of the most deleterious factors on astronaut’s health and is mainly attributed to an unbalance in the process of bone remodeling. Studies from the space microgravity have demonstrated that the disruption of bone remodeling is associated with the changes of four main functional bone cells, including osteoblast, osteoclast, osteocyte, and mesenchymal stem cells. For the limited availability, expensive costs and confined experiment conditions for conducting space microgravity studies, the mechanism of bone cells response and adaptation to microgravity is still unclear. Therefore, some ground-based simulated microgravity methods have been developed to investigate the bioeffects of microgravity and the mechanisms. Here, based on our studies and others, we review how bone cells (osteoblasts, osteoclasts, osteocytes and mesenchymal stem cells) respond and adapt to simulated microgravity.     

G-300, the first French Getaway Special microgravity measurements of fluid thermal conductivity

On Earth thermal conductivity measurements on liquids are difficult to perform because thermal motions due to convection. In microgravity the convection due to buoyancy is evanescent and we expect a strong lowering of Rayleigh and Nusselt numbers. Three low viscosity liquids are selected to carry out the measurements: distilled water (standard) and two silicone oils. We use a modified “hot plate” method with a simplified guard ring, the lowering of convective motions let us to use in the experimental cells larger interplate distances and/or temperature differences than in earth measurements so the accuracy must be improved. Comparisons between Earth and orbit results may help to understand the convection occurrence in our cells. G-300 payload is cantilevered from the experiment mounting plate (EMP) and it includes: four struts, an intermediate plate, a bottom plate with four bumpers, a battery box, an electronic rack and six experimental cells assembled in twin-packs thermally coupled with the EMP. Thermal, vibration and EMI tests have proved that the design satisfies the Nasa requirements and acceptance is in progress.     

Buoyancy effects on smoldering combustion

A theoretical and experimental study is carried out to determine the effect of buoyancy on the rate of spread of a cocurrent smolder reaction through a porous combustible material. Since buoyant forces are proportional to the product g(_gi − _g), they can be controlled experimentally by varying either the gravitational acceleration, g, or the density difference, _gi − _g. The latter approach was followed in the present work. Measurements are performed of the smolder spread rate through porous α-cellulose (0.83 void fraction) as a function of the ambient air pressure. The experiments are carried out in a pressure vessel for ambient pressures ranging from 0.5 to 1.2 atm. The rate of spread was obtained from the temperature histories of thermocouples placed at fixed intervals along the fuel centerline. The smolder velocity was found to increase as the ambient pressure was increased. Extinction was found to occur when the buoyancy forces could not overcome the drag forces, indicating that at least for the present experimental conditions transport by diffusion cannot, by itself, support the spread of a smolder reaction. This conclusion is particularly important for outer space conditions where gravity and consequently buoyancy could be negligible. In the analysis, which assumes one-dimensional processes, the transport equations are solved to give the smolder spread rate as a function of the inlet oxygen mass flux. This mass flux is then estimated by balancing buoyancy and drag forces. Assuming that the smolder chemical reaction is only weakly dependent on pressure, the analysis finally predicts a smolder velocity dependence of the form v Y_oig²_gi Pa², i.e. is proportional to the ambient pressure squared. Good qualitative agreement is found between the theoretical predictions and the experimental results.     

Modeled microgravity increases filamentation, biofilm formation, phenotypic switching, and antimicrobial resistance in Candida albicans

Candida albicans is an opportunistic fungal pathogen responsible for a variety of cutaneous and systemic human infections. Virulence of C. albicans increases upon exposure to some environmental stresses; therefore, we explored phenotypic responses of C. albicans following exposure to the environmental stress of low-shear modeled microgravity. Upon long-term (12-day) exposure to low-shear modeled microgravity, C. albicans transitioned from yeast to filamentous forms at a higher rate than observed under control conditions. Consistently, genes associated with cellular morphology were differentially expressed in a time-dependent manner. Biofilm communities, credited with enhanced resistance to environmental stress, formed in the modeled microgravity bioreactor and had a more complex structure than those formed in control conditions. In addition, cells exposed to low-shear modeled microgravity displayed phenotypic switching, observed as a near complete transition from smooth to "hyper" irregular wrinkle colony morphology. Consistent with the presence of biofilm communities and increased rates of phenotypic switching, cells exposed to modeled microgravity were significantly more resistant to the antifungal agent Amphotericin B. Together, these data indicate that C. albicans adapts to the environmental stress of low-shear modeled microgravity by demonstrating virulence-associated phenotypes.     

水下模拟失重试验模型设施发展综述

对国内外不同时期水下模型的组成、设计特点进行综述与分析。从国内外水下模型的发展来看,准确模拟人机界面是水下模型设备的研制要点。为简化建造过程,可以根据试验需求对水下模型的逼真度进行分级。设计中还需要考虑水下环境的适应性,例如合理选择材料和中性浮力设计。我国后续水下模型设备的设计可以从中借鉴有益经验。