Effects of rectilinear acceleration, caloric and optokinetic stimulation of human subjects in the Spacelab D-1 mission

A set of vestibular experiments was performed during the course of the German Spacelab D-1 mission from 30 October to 6 November 1985 by a consortium of experimenters from various european countries. Similar to the Spacelab SL-1 mission all of the scientific crew members were theoretically and practically trained for the experiments. Baseline measurements for all tests were collected 113, 86, 44, 30 and 18 days prior to the mission and compared with data taken inflight, on the landing day and the consecutive 7 to 14 days.

The hardware comprised mainly a motordriven accelerating platform, the SPACE SLED, and the vestibular helmet, a multi-purpose instrument in support of a variety of vestibular experiments including air-calorisation of the ears, optokinetic stimulation pattern presentation and optical and nystagmographic recording of eye movements.

Measurements of the threshold for the perception of detection of whole body movement did not reveal any dramatic changes in the 2 measured axes inflight when compared to preflight values. Early postflight values show a significantly elevated threshold for all axes in 3 out of 4 subjects.

The caloric nystagmus, already found during the SL-1 mission, was confirmed on all three tested subjects during the D-1 mission. It's amplitude and in some instances it's direction were influenced by horizontal acceleration on the SLED.

The amplitude of optokinetic nystagmus increased when subjects were allowed to free-float over that seen when subjects were fixed.

Stimulation of the neck receptors by roll movements of the body against the fixated head resulted in illusory object motion to the contralateral side. Torsional movements of the eyes during such neck receptor stimulation was present inflight and postflight, while it had not been observed preflight. Most results point to a reduction of otolithic effects in favour of visual and proprioceptive influences for spatial orientation.     

Changes of endolymphatic pressure in the semicircular canal of pigeon by caloric stimulation.

It gets into difficult to explain the mechanism of caloric nystagmus only by convection theory from results of microgravity experiments. One of the other theories is an occurrence of a relative volume change due to a temperature change. Since the volume change must lead to a pressure change after caloric stimulation, we tried to measure the ampulla pressure of the horizontal semicircular canal in pigeons (Columba livia) using an improved servo micropipette system. The main result was that the ampulla pressure increased by cooling and decreased by heating. The changes of the ampulla pressure depended on the temperature change but were not influenced by the pigeon's head position.     

Review of primary spaceflight-induced and secondary reloading-induced changes in slow antigravity muscles of rats.

We have examined the light and electron microscopic properties of hindlimb muscles of rats flown in space for 1-2 weeks on Cosmos biosatellite flights 1887 and 2044 and Space Shuttle missions Spacelab-3, Spacelab Life Sciences-1 and Spacelab Life Sciences-2. Tissues were obtained both inflight and postflight permitting definition of primary microgravity-induced changes and secondary reentry and gravity reloading-induced alterations. Spaceflight causes atrophy and expression of fast fiber characteristics in slow antigravity muscles. The stresses of reentry and reloading reveal that atrophic muscles show increased susceptibility to interstitial edema and ischemic-anoxic necrosis as well as muscle fiber tearing with disruption of contractile proteins. These results demonstrate that the effects of spaceflight on skeletal muscle are multifaceted, and major changes occur both inflight and following return to Earth's gravity.     

Determination of the threshold acceleration for the gravitropic stimulation of cress roots and hypocotyls.

To determine the range of the threshold acceleration (a-threshold) for the gravitropic stimulation of Lepidium sativum L. roots and hypocotyls, experiments were performed on a centrifuge-clinostat with two-orthogonal axes. The rotation rate of the clinostat was 4 rpm (< or = 1.8 x 10(-4) g), while that of the centrifuge was from 3 to 17 rpm (3 x 10(-3) to 10(-1) g). The gravitropic response was determined: (i) after growth of roots and hypocotyls in their normal vertical position and subsequent gravitropic stimulation for 3 h by accelerations of 4 x 10(-3) to 10(-1) g, and (ii) after continuous stimulation in the lateral direction by centripetal accelerations of 4 x 10(-3) to 10(-1) g. The a-threshold was defined by an extrapolation of the regression line of R = p + rx, where x was either ln a or l/a for 3 h or a continuous stimulation, respectively. The a-threshold estimated after 3 h stimulation was equal to 2.6 x 10(-3) g for roots and 3.1 x 10(-3) g for hypocotyls. The threshold accelerations that were unable to evoke a gravitropic response even with continuous stimulation of cress roots and hypocotyls were approximately 3.1 x 10(-3) g and 3.6 x 10(-3) g, respectively. Increasing the stimulation acceleration up to 4.1 x 10(-3) g led to a statistically confirmed gravitropic response of a definite proportion of both the root and hypocotyl populations. In the experiments where acceleration and stimulation time were variable, the threshold dose (D-threshold) for roots was determined to be about 14 to 22 g x s, depending on the stimulation duration and the range of accelerations. The kinetics of gravitropic response at a near-threshold acceleration (4 x 10(-3) to 1.9 x 10(-2) g) differed from that at 1 g (horizontal stimulation). At low forces, the maximal response dependent on the magnitude of acceleration could not be enhanced by increasing the stimulation time up to at least 210 min.     

Research on the adaptation of skeletal muscle to hypogravity: past and future directions.

Our current understanding of hypogravity-induced atrophy of skeletal muscles is based primarily on studies comparing pre- and post-flight properties of muscles. Interpretations are necessarily qualified by the assumption that the stress of reentry and readjustment to terrestrial gravity do not alter the parameters being analyzed. The neuromuscular system is highly responsive to changes in functional demands and capable of rapid adaptation, making this assumption questionable. A reexamination of the changes in the connective tissue and synaptic terminals of soleus muscles from rats orbited in biosatellites and sampled postflight indicates that these structural alterations represent adaptative responses of the atrophic muscles to the increased workload of returning to 1 G, rather than hypogravity per se. The atrophy of weightlessness is postulated to result because muscles are both underloaded and used less often. Proper testing of this hypothesis requires quantitation of muscle function by monitoring electromyography, force output and length changes during the flight. Experiments conducted in space laboratories, like those being developed for the Space Shuttle, will avoid the complications of reentry before tissue sampling and allow time course atudies of the rate of development of adaptive changes to zero gravity. Another area of great importance for future studies of muscle atrophy is inflight measurement of plasma levels of hormones and tissue receptor levels. Glucocorticoids, thyroid hormone and insulin exert dramatic regulatory influences on muscle structure. Prevention of neuromuscular atrophy becomes increasingly more important as spaceflights increase in duration. Definition of the atrophic mechanism is essential to developing means of preventing neuromuscular atrophy.     

基于双频GPS观测的简化动力学最小二乘批处理精密定轨

星载双频GPS载波相位和伪距观测量已成为低轨卫星获取精确三维位置和速度信息的主要方式. 本文以非差消电离载波相位和伪距组合作为观测量,应用简化动力学最小二乘批处理方法进行地球低轨卫星的精密定轨,并给出完整定轨流程. 采用逐段常量的经验加速度对动力学模型误差进行补偿,描述了经验加速度敏感矩阵及稀疏带状矩阵求逆的有效计算方法. 利用GRACE-A卫星GPS观测数据对定轨位置精度进行分析,结果显示,三维位置定轨精度优于5cm,经验加速度在径向、切向和法向上的补偿水平不超过40nm·s-2,大气阻力系数和辐射光压系数的估计值符合物理实际,星载接收机钟差大致呈线性并具有短周期小波动.      

Horizontal and vertical thermospheric cross-wind from GOCE linear and angular accelerations

Thermospheric wind measurements obtained from linear non-gravitational accelerations of the Gravity field and steady-state Ocean Circulation Explorer (GOCE) satellite show discrepancies when compared to ground-based measurements. In this paper the cross-wind is derived from both the linear and the angular accelerations using a newly developed iterative algorithm. The two resulting data sets are compared to test the validity of wind derived from angular accelerations and quantify the uncertainty in accelerometer-derived wind data. In general the difference is found to be less than 50?m/s vertically after high-pass filtering, and 100?m/s horizontally. A sensitivity analysis reveals that continuous thrusting is a major source of uncertainty in the torque-derived wind, as are the magnetic properties of the satellite. The energy accommodation coefficient is identified as a particularly promising parameter for improving the consistency of thermospheric cross-wind data sets in the future. The algorithm may be applied to obtain density and cross-wind from other satellite missions that lack accelerometer data, provided the attitude and orbit are known with sufficient accuracy.     

Balloon-borne,high-altitude gravimetry

Gravity measurements from a high-altitude balloon can verify global and upward-continued gravity models. A gravimeter suspended beneath a balloon is in a dynamic, and largely unpredictable, environment sensing accelerations due to gravity and balloon motions. Independent measurements of balloon motions using inertial navigation data combined with ground tracking data will allow for separation of balloon-induced accelerations from gravitational accelerations. Analysis of these data must estimate: 1) vertical gravimeter accelerations due to motion and gravity, 2) horizontal velocity to estimate the Eötvös effect, and 3) gravimeter position for comparison with gravity models. The first engineering test flight occurred on 11 October 1983, during the seasonal wind reversal and was very successful. Flight duration was approximately seven hours, with two hours of data collected at each of 30 km and 26 km altitudes. The results include gravity estimates, design criteria for future flights and feasibility analysis for vertical gravity profiles during ascent and descent.     

Influence of gravitoinertial force on vestibular nystagmus in man observed in a centrifuge.

The influence of gravity load on the vestibular system in man was investigated in a centrifuge operating on the free swing principle. The vertical vestibular nystagmus induced by acceleration to 3G was analyzed and compared with reference measurements during 1G. Our data indicate that the effects of increased gravity load include a prolonged decay time constant of upbeat nystagmus and a subject-dependent persisting upbeat nystagmus. In an attempt to explain these findings, an extension of the velocity storage model is proposed, with gravity as a second stimulus function in addition to angular acceleration.     

Remote sensing of the atmospheric from scientific stations “Salyut”

A review of the four-channel teleradiometer “Micron” calibration and data processing techniques has been presented. The sensor validation of the space-borne teleradiometer “Micron” was based on the following: the preflight absolute calibration with the state certificated standard, the secondary onboard standard (miniature filament lamp), the inflight measurements of the Moon's brightness. The procedure of the extinction coefficient determination from the daytime horizon profiles has been developed. The validity of the used techniques was estimated by carring out model calculations. An optimal measuring procedure has been recommended. The proposed simple techniques are used for processing the space measured horizon brightness data. The results have been compared with the earlier published ones.     

Performance of GPS-based accelerometry: A simulation experiment

Recent studies have shown that with the availability of high-quality CHAMP and GRACE gravity field models, it is feasible to determine accurate non-gravitational accelerations for low Earth orbiting satellites indirectly from precise GPS satellite-to-satellite observations. Possible applications of this so-called GPS-based accelerometry approach consist of accelerometer calibration and atmospheric density and wind computations. With the growing number of high-quality space-borne GPS receivers, this method could be applied to a large range of satellites. In this paper an extensive simulation study has been carried out, based on real accelerometer data from the GRACE mission, in order to determine the optimal processing strategy and the resulting accuracy of the estimated non-gravitational accelerations. It is shown that the optimal processing strategy consists of a piecewise linear parameterization of the estimated empirical accelerations, together with short 6-h orbit arcs. The GPS-based accelerometry approach makes use of triple-differenced GPS observations and the impact of considering the correlated observation noise was found to be marginal in the presence of other error sources such as GPS ephemeris errors. Using a priori non-gravitational force models improves the recovery of low temporal resolution accelerations, except during huge geomagnetic storms. With this strategy, non-gravitational accelerations can be recovered during high solar activity with an accuracy of better than 10% of the total signal in along-track direction and around 25–40% in cross-track direction, at time resolutions of around 8–20 min. During solar minimum conditions, the relative recovery error will increase to approximately 50% in along-track direction and around 60–70% in cross-track direction, due to the reduced atmospheric drag signal. Unfortunately, GPS-based accelerometry is hardly sensitive in the radial direction.     

Early development in aquatic vertebrates in near weightlessness during the D-2 Mission STATEX project.

Aboard the German-Spacelab-Mission D-2 the project "Gravity Perception and Neuronal Plasticity (STATEX II)" was performed. STATEX is for STATolith EXperiment. Objects were growing tadpoles of the South African Toad (Xenopus laevis D.) and a juvenile cichlid fish (Oreochromis mossambicus). The results give a broader base for the understanding of how environmental stimuli (e.g. linear accelerations) affect the development and function of the gravity perceiving systems in these two vertebrates. These systems are accepted as models for the human vestibulum. Results of experiments in hyper-g (up to 5 g), simulated weightlessness (Fast-rotating-clinostat) and parabolic flights are compared and discussed.     

Growth protocols for etiolated soybeans germinated within BRIC-60 canisters under spaceflight conditions.

As part of the GENEX (Gene Expression) spaceflight experiment, protocols were developed to optimize the inflight germination and subsequent growth of 192 soybean (Glycine max cv McCall) seeds during STS-87. We describe a method which provided uniform growth and development of etiolated seedlings while eliminating root and shoot restrictions for short-term (4-7 day) experiments. Final seedling growth morphologies and the gaseous CO2 and ethylene levels present both on the last day in space and at the time of recovery within the spaceflight and ground control BRIC-60 canisters are presented.     

多功能惯性敏感仪DTAG的力学分析

在干式调谐挠性陀螺仪DTG**的基础上,使转子具有适当的不等转动惯量及转子的轴向摆性,可将DTG改进成一种多功能惯性敏感仪DTAG.它可以敏感正交轴向的角速度和线加速度.本文建立了DTAG的力学模型,导出了方程的稳态解.从力学分析的角度证明了DTAG实现多功能的可行性. 另外,若仅就DTG而言解决了用单环结构消除2N角振动引起常值漂移的问题.      

The influence of space flight factors on viability and mutability of plants.

The experiments with air-dried Crepis capillaris seeds aboard the Soyuz 16 spaceship and the orbital stations Salyut 5, 6, 7 have revealed an increase in the frequency of aberrant cells in seedlings grown from flight-exposed seeds during the flight (experiment) and after the flight on Earth (flight control) as compared to the ground-based control. The increase in seedlings grown during the flight is more significant than in the flight control. During the flight Arabidopsis thaliana developed from cotyledons to the flowering stage. Analysis of seeds setting on these plants after the flight has shown a reduction in the fertility of these plants and an increase in the frequency of recessive mutants ("Light block-1"). An increased frequency of mutants was also retained in the progeny of plants which had passed through a complete cycle of development during the flight ("Fiton-3"). Suppression of embryo viability was observed in all experiments and expressed itself in reduced germinating ability of seeds from the exposed plants and in the early death of seedlings. Damages resulting from chromosome aberrations are eliminated in the first postflight generation and damages resulting from gene mutations and micro-aberrations are preserved for a longer time.     

Effects of altered gravity on the swimming behaviour of fish.

Humans taking part in parabolic aircraft flights (PAFs) may suffer from space motion sickness-phenomena (SMS, a kinetosis). It has been argued that SMS during PAFs might not be based on microgravity alone but rather on changing accelerations from 0 g to 2 g. We test here the hypothesis that PAF-induced kinetosis is based on asymmetric statoliths (i.e., differently weighed statoliths on the right and the left side of the head), with asymmetric inputs to the brain being disclosed at microgravity. Since fish frequently reveal kinetotic behaviour during PAFs (especially so-called spinning movements and looping responses), we investigated (1) whether or not kinetotically swimming fish at microgravity would have a pronounced inner ear otolith asymmetry and (2) whether or not slow translational and continuously changing linear (vertical) acceleration on ground induced kinetosis. These latter accelerations were applied using a specially developed parabel-animal-container (PAC) to stimulate the cupular organs. The results suggest that the fish tested on ground can counter changing accelerations successfully without revealing kinetotic swimming patterns. Kinetosis could only be induced by PAFs. This finding suggests that it is indeed microgravity rather than changing accelerations, which induces kinetosis. Moreover, we demonstrate that fish swimming kinetotically during PAFs correlates with a higher otolith asymmetry in comparison to normally behaving animals in PAFs.     

Particle and optical measurements aboard the Aureol 3 spacecraft (ARCAD 3 project): A — The particle experiments B — Optical measurements

The CESR Toulouse - IKI Moscow particle instrument package aboard the AUREOL-3 satellite consists of a complete set of charged particle spectrometers which measure electron and ion fluxes from 15 eV to 25 keV in 128 steps and in 11 directions. In addition, 4 channel spectrometers (2 electron and 2 ion channels in parallel) allow high time resolution measurements (up to 10 msec) with onboard calculation of auto and cross correlation functions. For higher energies (40 – 280 keV), solid-state spectrometers are used to measure electron and proton fluxes in 4 channels in parallel. In addition, two Geiger counters are used for the determination of the trapping boundaries. Two mass-energy ion spectrometers (1 to 32 A.M.U., 0.02 – 15 keV) are placed with viewing angles which allow a distinction between nearly isotropic auroral proton precipitation and conical beams accelerated in the auroral ionosphere. Auroral and airglow photometry is performed aboard the AUREOL-3 satellite by a set of 3 parallel directed photometers with tiltable interference filters for 6300 Å, 4278 Å and Doppler shifte H_β emissions. Various modes of energy, angular and mass scanning, correlation function calculation and various Soviet and French telemetry regimes provide the possibility of choosing the sequences of measurements according to particular experimental programs along the orbit. Finally, examples of data from inflight measurements using the above instruments are presented and briefly discussed, showing several interesting features.     

Simulation of launch and re-entry acceleration profiles for testing of shuttle and unmanned microgravity research payloads

Microgravity experiments designed for execution in Get-Away Special canisters, Hitchhiker modules, and Reusable Re-entry Satellites will be subjected to launch and re-entry accelerations. Crew-dependent provisions for preventing acceleration damage to equipment or products will not be available for these payloads during flight; therefore, the effects of launch and re-entry accelerations on all aspects of such payloads must be evaluated prior to flight. A procedure was developed for conveniently simulating the launch and re-entry acceleration profiles of the Space Shuttle (3.3 and 1.7 × g maximum, respectively) and of two versions of NASA's proposed materials research Re-usable Re-entry Satellite (8 × g maximum in one case and 4 × g in the other). By using the 7 m centrifuge of the Gravitational Plant Physiology Laboratory in Philadelphia it was found possible to simulate the time dependence of these 5 different acceleration episodes for payload masses up to 59 kg. A commercial low-cost payload device, the “Materials Dispersion Apparatus” of Instrumentation Technology Associates was tested for (1) integrity of mechanical function, (2) retention of fluid in its compartments, and (3) integrity of products under simulated re-entry g-loads. In particular, the sharp rise from 1 g to maximum g-loading that occurs during re-entry in various unmanned vehicles was successfully simulated, conditions were established for reliable functioning of the MDA, and crystals of 5 proteins suspended in compartments filled with mother liquor were subjected to this acceleration load.     

Gravisensitivity of cress roots.

The minimum dose (stimulus x time [gs]) eliciting a visible gravitropic response, has been determined using continuous and intermittent stimulation and two different accelerations at 1 g and 0.l g. The minimum dose of 20-30 gs estimated for microgravity roots and of 50-60 gs for roots grown on a 1 g-centrifuge indicated a higher sensitivity of microgravity roots. Applying intermittent stimuli to microgravity-grown roots, gravitropic responses were observed after two stimuli of 13.5 gs separated by a stimulus free interval of 118 s. The curvature of microgravity-grown roots to lateral stimulation by 0.1 g was remarkably smaller than by 1g in spite of the same doses which were applied to the seedlings. Microscopic investigations corresponding to stimulations in the range of the threshold values, demonstrated small displacement (< 2 micrometers) of statoliths in root statocytes. Accepting the statolith theory, one can conclude that stimulus transformation has to occur in the cytoplasm in close vicinity to the statoliths and that this transformation system was affected during seedling cultivation in microgravity.     

Measurement of the partial oxygen pressure and oxygen utilization in the skin of cosmonauts aboard Salyut 6

The oxygen tension (PO₂) in the dorsal skin surface of the forearm was studied during the stay of cosmonauts on board Salyut 6. Between the fourth and fifth day of stay on the orbital station a considerable reduction of the PO₂ level was observed. The oxygen utilization values were also reduced. In the early postflight period the low PO₂ level persisted, with gradual normalization.