Expression of p53-regulated genes in human cultured lymphoblastoid TSCE5 and WTK1 cell lines after spaceflight in a frozen state

The 53 kDa tumor suppressor protein p53 is generally thought to contribute to the genetic stability of cells and to protect cells from DNA damage through the activity of p53-centered signal transduction pathways. To clarify the effect of space radiation on the expression of p53-dependent regulated genes, gene expression profiles were compared between two human cultured lymphoblastoid cell lines: one line (TSCE5) has a wild-type p53 gene status, and the other line (WTK1) has a mutated p53 gene status. Frozen human lymphoblastoid cells were stored in a freezer in the International Space Station (ISS) for 133 days. Gene expression was analyzed using DNA chips after culturing the space samples for 6 h on the ground after their return from space. Ground control samples were also cultured for 6 h after being stored in a frozen state on the ground for the same time period that the frozen cells were in space. p53-Dependent gene expression was calculated from the ratio of the gene expression values in wild-type p53 cells and in mutated p53 cells. The expression of 50 p53-dependent genes was up-regulated, and the expression of 94 p53-dependent genes was down-regulated after spaceflight. These expression data identified genes which could be useful in advancing studies in basic space radiation biology. The biological meaning of these results is discussed from the aspect of gene functions in the up- and down-regulated genes after exposure to low doses of space radiation.     

Depth dose measurements with the Liulin-5 experiment inside the spherical phantom of the MATROSHKA-R project onboard the International Space Station

The Liulin-5 experiment is a part of the international project MATROSHKA-R on the Russian segment of the ISS, which uses a tissue-equivalent spherical phantom equipped with a set of radiation detectors. The objective of the MATROSHKA-R project is to provide depth dose distribution of the radiation field inside the sphere in order to get more information on the distribution of dose in a human body. Liulin-5 is a charged particle telescope using three silicon detectors. It measures time resolved energy deposition spectra, linear energy transfer (LET) spectra, particle flux, and absorbed doses of electrons, protons and heavy ions, simultaneously at three depths along the radius of the phantom. Measurements during the minimum of the solar activity in cycle 23 show that the average absorbed daily doses at 40 mm depth in the phantom are between 180 μGy/day and 220 μGy/day. The absorbed doses at 165 mm depth in the phantom decrease by a factor of 1.6–1.8 compared to the doses at 40 mm depth due to the self-shielding of the phantom from trapped protons. The average dose equivalent at 40 mm depth is 590 ± 32 μSV/day and the galactic cosmic rays (GCR) contribute at least 70% of the total dose equivalent at that depth. Shown is that due to the South Atlantic Anomaly (SAA) trapped protons asymmetry and the direction of Liulin-5 lowest shielding zone the dose rates on ascending and descending nodes in SAA are different. The data obtained are compared to data from other radiation detectors on ISS.     

The extension of the INCL model for simulation of shielding in space

Radiation hazard for space missions is mainly due to cosmic ray protons, helium nuclei and light ions, whose energy spectrum is maximum around 1 GeV per nucleon but remains non-negligible for energies up to 15 GeV per nucleon. Nuclear reactions induced by high energy protons are often described by intranuclear cascade plus evaporation models. The attention is focused here on the Liège Intranuclear Cascade model (INCL), which has been shown to reproduce fairly well a great deal of experimental data for nucleon-induced reactions in the 200 MeV to 2 GeV range, when coupled with the ABLA evaporation-fission code. In order to extend the model to other conditions relevant for space radiation, three improvements of INCL are under development. They are reported on here. First, the reaction model has been extended to nucleon–nucleus reactions at incident energies up to 15 GeV, mainly by the inclusion of additional pion production channels in nucleon–nucleon collisions during the cascade. Second, a coalescence mechanism for the emission of light charged particles has been implemented recently. Finally, the model has been modified in order to accommodate light ions as projectiles. First results are shown and compared with illustrative experimental data. Implications for issues concerning radiation protection in space are discussed.     

Solar proton events recorded in the stratosphere during cosmic ray balloon observations in 1957–2008

Long-term balloon observations have been performed by the Lebedev Physical Institute since 1957 up to the present time. The observations are taken several times a week at the polar and mid latitudes and allow us to study dynamics of galactic and solar cosmic ray as well as secondary particle fluxes in the atmosphere and in the near-Earth space. Solar energetic particles (120) – mostly protons – (SEP) events with >100 MeV proton intensity above 1 cm⁻² s⁻¹ s⁻¹ were recorded during 1958–2006. Before the advent of the SEP monitoring on spacecraft these results constituted the only homogeneous series of >100 MeV SEP events. The SEP intensities and energy spectra inferred from the Lebedev Physical Institute observations are consistent with the results taken in the adjacent energy intervals by the spacecraft and neutron monitors. Joint consideration of the SEP events series recorded by balloons and by neutron monitors during solar cycles 20–23 makes it possible to restore the probable number of events in solar cycle 19, which was not properly covered by observations. Some correlation was found between duration of SEP event production in a solar cycle and sunspot cycle characteristics.     

High energy charged particle experiment – A payload proposal to KuaFu-B mission

High Energy Charged Particle Experiment (HECPE) is to measure the fluxes of MeV electrons and tens of MeV protons. The two satellites of KuaFu-B are in the same polar orbit with apogee 7.0R_E, perigee 1.8R_E. They can sweep large L values and pass through the inner and outer radiation belts. The high energy electrons and protons in the radiation belts are principal sources for failures of satellites and spacecrafts in the Earth orbits. The enhancements of the high energy electrons and protons, so-called energetic particle events, are important phenomena of the Space Weather. The energy ranges monitored by HECPE are 0.3–0.5 MeV, 0.5–1.0 MeV, 1.0–2.0 MeV, and E > 2.0 MeV for electrons, 5–10 MeV, 10–20 MeV, 20–40 MeV, and 40–80 MeV for protons.     

Analysis of the space radiation doses obtained simultaneously at two different locations outside the ISS

Space weather and related ionizing radiation has been recognized as one of the main health concerns for the International Space Station (ISS) crew. The estimation of the radiation effect on humans outside the ISS requires at first order accurate knowledge of their accumulated absorbed dose rates, which depend on the global space radiation distribution, solar cycle and local variations generated by the 3D mass distribution surrounding the ISS. The R3DE (Radiation Risks Radiometer-Dosimeter for the EXPOSE-E platform) on the European Technological Exposure Facility (EuTEF) worked successfully outside of the European Columbus module between February 2008 and September 2009. A very similar instrument named R3DR for the EXPOSE-R platform worked outside the Russian Zvezda module of the ISS between March 2009 and August 2010. Both are Liulin-type detectors, Bulgarian-built miniature spectrometer-dosimeters. The acquired approximately 5 million deposited energy spectra from which the flux and absorbed dose rate were calculated with 10 s resolution behind less than 0.41 g cm⁻² shielding. This paper analyses the spectra collected in 2009 by the R3DE/R instruments and the long-term variations in the different radiation environments of Galactic Cosmic Rays (GCR), inner radiation belt trapped protons in the region of the South Atlantic Anomaly (SAA) and relativistic electrons from the Outer Radiation Belt (ORB). The R3DE instrument, heavily shielded by the surrounding structures, measured smaller primary fluxes and dose rates from energetic protons from the SAA and relativistic electrons from the ORB but higher values from GCRs because of the contribution from secondary particles. The main conclusion from this investigation is that the dose rates from different radiation sources around the International Space Station (ISS) have a large special and temporal dynamic range. The collected data can be interpreted as possible doses obtained by the cosmonauts and astronauts during Extra Vehicular Activities (EVA) because the R3DE/R instruments shielding is very similar to the Russian and American space suits average shielding (,  and ). Fast, active measurements are required to assess accurately the dose accumulated by astronauts during EVA.     

Energy spectrum of proton flux near geomagnetic equator at low altitudes

The results of proton energy (tens keV – several MeV) spectrum measurements near geomagnetic equator (L < 1.15) at low altitudes (<1000 km) are presented. We used data of experiments onboard ACTIVE, SAMPEX, NOAA TIROS-N satellites and SPRUT-VI (MIR station) and cover a time range of about 30 years (including previous measurements). It was found that the kappa-distribution function fits the experimental spectrum with the best correlation coefficient. A comparison of energy spectra of near-equatorial protons and ring-current protons was made. Using the estimation of the life time of near-equatorial protons we explain the difference in spectral indices of radiation belt and near-equatorial proton formation. We conclude that the ring current is the main source of the near-equatorial protons.     

Simulated microgravity inhibits the proliferation of K562 erythroleukemia cells but does not result in apoptosis

Astronauts and experimental animals in space develop the anemia of space flight, but the underlying mechanisms are still unclear. In this study, the impact of simulated microgravity on proliferation, cell death, cell cycle progress and cytoskeleton of erythroid progenitor-like K562 leukemia cells was observed. K562 cells were cultured in NASA Rotary Cell Culture System (RCCS) that was used to simulate microgravity (at 15 rpm). After culture for 24 h, 48 h, 72 h, and 96 h, the cell densities cultured in RCCS were only 55.5%, 54.3%, 67.2% and 66.4% of the flask-cultured control cells, respectively. The percentages of trypan blue-stained dead cells and the percentages of apoptotic cells demonstrated no difference between RCCS-cultured cells and flask-cultured cells at every time points (from 12 h to 96 h). Compared with flask-cultured cells, RCCS culture induced an accumulation of cell number at S phase concomitant with a decrease at G0/G1 and G2/M phases at 12 h. But 12 h later (from 24 h to 60 h), the distribution of cell cycle phases in RCCS-cultured cells became no difference compared to flask-cultured cells. Consistent with the changes of cell cycle distribution, the levels of intercellular cyclins in RCCS-cultured cells changed at 12 h, including a decrease in cyclin A, and the increasing in cyclin B, D1 and E, and then (from 24 h to 36 h) began to restore to control levels. After RCCS culture for 12–36 h, the microfilaments showed uneven and clustered distribution, and the microtubules were highly disorganized. These results indicated that RCCS-simulated microgravity could induce a transient inhibition of proliferation, but not result in apoptosis, which could involve in the development of space flight anemia. K562 cells could be a useful model to research the effects of microgravity on differentiation and proliferation of hematopoietic cells.     

Anomalous phosphenes in ocular protontherapy

We have undertaken a clinical ground study of proton-induced light flashes (phosphenes). Patients treated at the Institut Curie – Centre de Protonthérapie in Orsay, France, received radiation therapy to cure ocular and skull-base cancers. Sixty percent of the patients treated for choroidal melanomas using 73 MeV protons report anomalous phosphenes. Delivering a radiation dose on the retina only is not sufficient to trigger the light flash. The present study may be the first indication of phosphenes triggered by protons of few tens of MeV.     

Early effects of low dose C ion or X-ray irradiation on human peripheral blood lymphocytes

The aim of this study was to estimate the acute effects of low dose ¹²C⁶⁺ ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05 Gy ¹²C⁶⁺ ions or X-ray radiation and cell responses were measured at 24 h after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supernatant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-γ and TNF-α in HPBL and their protein levels in supernatant were significantly increased at 24 h after exposure to 0.05 Gy ¹²C⁶⁺ ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05 Gy high linear energy transfer (LET) ¹²C⁶⁺ radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDI.     

The heliospheric modulation of cosmic rays: Effects of a latitude dependent solar wind termination shock

Observations made with the two Voyager spacecraft confirmed that the solar wind decelerates to form the heliospheric termination shock and that it has begun its merger with the local interstellar medium. The compression ratio of this shock affects galactic cosmic rays when they enter the heliosphere. Hydrodynamic (HD) models show that the compression ratio can have a significant latitude dependence; with the largest value in the nose direction of the heliosphere, becoming significantly less towards the polar regions. The modulation effects of such large latitude dependence are studied, using a well-established numerical drift and shock modulation model. We focus on computing the modulated spectra for galactic protons with emphasis on the radial and polar gradients in the equatorial plane and at a polar angle of θ = 55°, corresponding to the heliolatitude of Voyager 1. Two sets of solutions are computed and compared each time; with and without a latitude dependence for the compression ratio. All computations are done for the two magnetic field polarity cycles assuming solar minimum conditions. Including the termination shock in the model allows the study of the re-acceleration of galactic protons in the outer heliosphere. We find that for the A < 0 polarity cycle the intensity between ∼200 MeV and ∼1 GeV in the vicinity of the shock in the heliospheric equatorial plane may exceed the local interstellar value specified at the heliopause. Unfortunately, at θ = 55°, the effect is reduced. This seems not possible during an A > 0 cycle because significant modulation is then predicted between the heliopause and the termination shock, depending on how strong global gradient and curvature drifts are in the heliosheath. The overall effect of the shock on galactic protons in the equatorial plane is to reduce the total modulation as a function of radial distance with respect to the interstellar spectrum. Making the compression ratio latitude dependent enhances these effects at energies E < 200 MeV in the equatorial plane. At larger heliolatitudes these effects are even more significant. The differences in the modulation between the two drift cycles are compelling when the compression ratio is made latitude dependent but at Earth this effect is insignificant. A general result is that the computed radial gradient changes for galactic protons at and close to the TS and that these changes are polarity dependent. In line with previous work, large polarity dependent effects are predicted for the inner heliosphere and also close to the shock’s position in the equatorial plane. In contrast, at θ = 55°, the largest polarity effect occurs in the middle heliosphere (50 AU), enhanced by the latitude dependence of the compression ratio. At this latitude, the amount of proton modulation between the heliopause and the termination shock is much reduced. If galactic cosmic rays were to experience some diffusive shock acceleration over the 100–1000 MeV range at the shock, the radial gradient should change its sign in the vicinity of the shock, how large, depends on the compression ratio and the amount of drifts taking place in the outer heliosphere. The effective polar gradient shows a strong polarity dependence at Earth but this dissipates at θ = 55°, especially with increasing radial distance. This tendency is enhanced by making the compression ratio latitude dependent.     

Cosmic ray detection on the Foton-M2 satellite by a track etch detector stack

In the frame of the European Space Agency (ESA) project called “Biology and Physics in Space”, the returning satellite, Foton-M2, carried an open-to-space exposure platform outside of the satellite body, called as BIOPAN-5, loaded with exo-biological experiments and facilities for radiation dosimetry (RADO). One of the RADO experiments was dedicated to the detection of the primary galactic cosmic rays (GCR) and secondary neutrons by a track etch detector stack. The daily absorbed dose (D) and dose equivalent (H) were calculated from the experimental LET spectra (LET > 10 keV/μm). Under a shielding of ∼2.8 g/cm² the averaged H was found to be 658 ± 8 μSv/d, with a quality factor (Q) of 6.2 ± 1.2. The LET spectra showed a local peak at ∼105 keV/μm suggesting that the majority of tracks were created by trapped protons as it has been predicted by calculations. The low LET dose of the cosmic radiation was determined by 4 TLD stacks, and the total dose was found to be 795 ± 14 μSv/d.     

R3D-B2 – Measurement of ionizing and solar radiation in open space in the BIOPAN 5 facility outside the FOTON M2 satellite

Solar and space radiation have been monitored using the R3D-B2 radiation risks radiometer-dosimeter on board a recent space flight on the Russian satellite Foton M2 within the ESA Biopan 5 facility mounted on the outside of the satellite exposed to space conditions. The solar radiation has been assayed in four wavelength bands (UV-C, 170–280 nm, UV-B, 280–315 nm), UV-A (315–400 nm) and PAR (photosynthetic active radiation, 400–700 nm). The data show an increasing tumbling rotation of the satellite during the mission. The photodiodes do not show a cosine response to the incident light which has been corrected. After calibration of the signals using the extraterrestrial spectrum, doses have been calculated for each orbit, for each day and for the total mission as basic data for the biological material which has been exposed in parallel in the Biopan facility. Cosmic ionizing radiation has been monitored and separated in 256 deposited energy spectra, which were further used for determination of the absorbed dose rate and flux. Basic data tables were prepared to be used by other Biopan 5 experiments. The paper summarizes the results for the Earth radiation environment at the altitude (262–304 km) of the Foton M2 spacecraft. Comparisons with the predictions of NASA Earth radiation environment experimental models AE-8 and AP-8, and the PSB97 model are also presented, which calculate the fluxes of ionizing radiation from a simulation. AP-8 is a model for trapped radiation.     

Moon surface radiation environment analysis for February 1956 solar event conditions

The radiation environment on the surface of the Moon presents a new source of particles resulting from the interaction of incoming solar protons and galactic cosmic rays with the lunar regolith. Here we present a study of the fluence profile of primary and secondary particles on the top 1 m layer of lunar regolith for the spectrum of one of the hardest spectrum solar event, that of February 1956. Different regolith compositions and their influence in proton and neutron production and backscattering is considered, as well as the nature of the backscattered radiation. Simple geometry Monte Carlo simulations have been used also for calculating regolith shielding properties, and it is shown that a layer of at least 50 cm regolith is needed for significantly reducing the dose levels received by astronauts in a hypothetical lunar habitat.     

Modelling of galactic Carbon in an asymmetrical heliosphere: Effects of asymmetrical modulation conditions

Observations of galactic cosmic rays (GCRs) from the two Voyager spacecraft inside the heliosheath indicate significant differences between them, suggesting that in addition to a possible global asymmetry in the north–south dimensions (meridional plane) of the heliosphere, it is also possible that different modulation (turbulence) conditions could exist between the two hemispheres of the heliosphere. We focus on illustrating the effects on GCR Carbon of asymmetrical modulation conditions combined with a heliosheath thickness that has a significant dependence on heliolatitude. To reflect different modulation conditions between the two heliospheric hemispheres in our numerical model, the enhancement of both polar and radial perpendicular diffusion off the ecliptic plane is assumed to differ from heliographic pole to pole. The computed radial GCR intensities at polar angles of 55° (approximating the Voyager 1 direction) and 125° (approximating the Voyager 2 direction) are compared at different energies and for both particle drift cycles. This is done in the context of illustrating how different values of the enhancement of both polar and radial perpendicular diffusion between the two hemispheres contribute to causing differences in radial intensities during solar minimum and moderate maximum conditions. We find that in the A > 0 cycle these differences between 55° and 125° change both quantitatively and qualitatively for the assumed asymmetrical modulation condition as reflected by polar diffusion, while in the A < 0 cycle, minute quantitative differences are obtained. However, when both polar and radial perpendicular diffusion have significant latitude dependences, major differences in radial intensities between the two polar angles are obtained in both polarity cycles. Furthermore, significant differences in radial intensity gradients obtained in the heliosheath at lower energies may suggest that the solar wind turbulence at and beyond the solar wind termination shock must have a larger latitudinal dependence.     

Cosmic ray anisotropies in the outer heliosphere

The observation of the directional distribution of energetic and cosmic ray particles has been done with the Voyager spacecraft over a long period. Since 2002, when the first flux enhancements of charged particles associated with the approach of Voyager 1 to the solar wind termination shock were observed, these anisotropy measurements have become of special interest. They play an important role to understand the magnetic field and shock structure and the basics of the modulation of cosmic ray and anomalous particles at and beyond the termination shock. They also serve as motivation to study the spatial behavior of galactic and anomalous cosmic ray anisotropies with numerical modulation models in order to illustrate how the radial anisotropy, at different energies, change from upstream to downstream of the termination shock. Observations made by Voyager 1 indicate that the termination shock is a complicated region than previously thought, hence the effects of the latitude dependence of the termination shock’s compression ratio and injection efficiency on the radial anisotropies of galactic and anomalous protons will be illustrated. We find that the magnitude and direction of the radial anisotropy strongly depends on the position in the heliosphere and the energy of particles. The effect of the TS on the radial anisotropy is to abruptly increase its value in the heliosheath especially in the A > 0 cycle for galactic protons and in both polarity cycles for anomalous protons. Furthermore, the global effect of the latitude dependence of the shock’s compression ratio is to increase the radial anisotropy for galactic protons throughout the heliosphere, while when combined with the latitude dependence of the injection efficiency this increase depends on modulation factors for anomalous protons and can even alter the direction of the radial anisotropy.     

Space Shuttle drops down the SAA doses on ISS

Long-term analysis of data from two radiation detection instruments on the International Space Station (ISS) shows that the docking of the Space Shuttle drops down the measured dose rates in the region of the South Atlantic Anomaly (SAA) by a factor of 1.5–3. Measurements either by the R3DE detector, which is outside the ISS at the EuTEF facility on the Columbus module behind a shielding of less than 0.45 g cm⁻², and by the three detectors of the Liulin-5 particle telescope, which is inside the Russian PEARS module in the spherical tissue equivalent phantom behind much heavier shielding demonstrate that effect. Simultaneously the estimated averaged incident energies of the incoming protons rise up from about 30 to 45 MeV. The effect is explained by the additional shielding against the SAA 30–150 MeV protons, provided by the 78 tons Shuttle to the instruments inside and outside of the ISS. An additional reason is the ISS attitude change (performed for the Shuttle docking) leading to decreasing of dose rates in two of Liulin-5 detectors because of the East–West proton fluxes asymmetry in SAA. The Galactic Cosmic Rays dose rates are practically not affected.