Low-temperature ionizing radiation resistance of Deinococcus radiodurans and Antarctic Dry Valley bacteria

The high flux of cosmic rays onto the unshielded surface of Mars poses a significant hazard to the survival of martian microbial life. Here, we determined the survival responses of several bacterial strains to ionizing radiation exposure while frozen at a low temperature characteristic of the martian near-subsurface. Novel psychrotolerant bacterial strains were isolated from the Antarctic Dry Valleys, an environmental analogue of the martian surface, and identified by 16S rRNA gene phylogeny as representatives of Brevundimonas, Rhodococcus, and Pseudomonas genera. These isolates, in addition to the known radioresistant extremophile Deinococcus radiodurans, were exposed to gamma rays while frozen on dry ice (-79°C). We found D. radiodurans to exhibit far greater radiation resistance when irradiated at -79°C than was observed in similar studies performed at higher temperatures. This greater radiation resistance has important implications for the estimation of potential survival times of microorganisms near the martian surface. Furthermore, the most radiation resistant of these Dry Valley isolates, Brevundimonas sp. MV.7, was found to show 99% 16S rRNA gene similarity to contaminant bacteria discovered in clean rooms at both Kennedy and Johnson Space Centers and so is of prime concern to efforts in the planetary protection of Mars from our lander probes. Results from this experimental irradiation, combined with previous radiation modeling, indicate that Brevundimonas sp. MV.7 emplaced only 30?cm deep in martian dust could survive the cosmic radiation for up to 100,000 years before suffering 10? population reduction.     

Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission

Spore-forming bacteria are of particular concern in the context of planetary protection because their tough endospores may withstand certain sterilization procedures as well as the harsh environments of outer space or planetary surfaces. To test their hardiness on a hypothetical mission to Mars, spores of Bacillus subtilis 168 and Bacillus pumilus SAFR-032 were exposed for 1.5 years to selected parameters of space in the experiment PROTECT during the EXPOSE-E mission on board the International Space Station. Mounted as dry layers on spacecraft-qualified aluminum coupons, the "trip to Mars" spores experienced space vacuum, cosmic and extraterrestrial solar radiation, and temperature fluctuations, whereas the "stay on Mars" spores were subjected to a simulated martian environment that included atmospheric pressure and composition, and UV and cosmic radiation. The survival of spores from both assays was determined after retrieval. It was clearly shown that solar extraterrestrial UV radiation (λ≥110?nm) as well as the martian UV spectrum (λ≥200?nm) was the most deleterious factor applied; in some samples only a few survivors were recovered from spores exposed in monolayers. Spores in multilayers survived better by several orders of magnitude. All other environmental parameters encountered by the "trip to Mars" or "stay on Mars" spores did little harm to the spores, which showed about 50% survival or more. The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation. These results will have implications for planetary protection considerations.     

A call for proactive xenoarchaeological guidelines – Scientific, policy and socio-political considerations

As planetary exploration advances, the likelihood of encountering suspected artifacts of astrobiological activity increases, which, it is argued, should be investigated under the auspices of proto-scientific xenoarchaeology. Considering both the unfavorable conditions under which such an investigation may be undertaken (e.g., while observing international planetary protection protocols, utilizing remote sensing techniques in exotic pressure, temperature, chemical, and gravity environments, or adhering to stringent terrestrial biological quarantine and security measures) as well as the demonstrated propensity of the social mind to romanticize or mythologize even the most benign planetary landforms, it is clear that a reactive and poorly preconceived xenoarchaeological methodology will be plagued by inaccuracies, rushed judgments, unrealized bias, misinformation, and erroneous conclusions, along with the negative socio-political impacts that accompany them. Central principles for establishing a rigorous xenoarchaeological methodology are proposed, scientific and technical difficulties are discussed, pertinent international protocols and agreements are reviewed, and sociological and historical considerations are explored.     

Effects of simulated space radiation on immunoassay components for life-detection experiments in planetary exploration missions

Abstract The Life Marker Chip (LMC) instrument is part of the proposed payload on the ESA ExoMars rover that is scheduled for launch in 2018. The LMC will use antibody-based assays to detect molecular signatures of life in samples obtained from the shallow subsurface of Mars. For the LMC antibodies, the ability to resist inactivation due to space particle radiation (both in transit and on the surface of Mars) will therefore be a prerequisite. The proton and neutron components of the mission radiation environment are those that are expected to have the dominant effect on the operation of the LMC. Modeling of the radiation environment for a mission to Mars led to the calculation of nominal mission fluences for proton and neutron radiation. Various combinations and multiples of these values were used to demonstrate the effects of radiation on antibody activity, primarily at the radiation levels envisaged for the ExoMars mission as well as at much higher levels. Five antibodies were freeze-dried in a variety of protective molecular matrices and were exposed to various radiation conditions generated at a cyclotron facility. After exposure, the antibodies' ability to bind to their respective antigens was assessed and found to be unaffected by ExoMars mission level radiation doses. These experiments indicated that the expected radiation environment of a Mars mission does not pose a significant risk to antibodies packaged in the form anticipated for the LMC instrument. Key Words: Life-detection instruments-Planetary habitability and biosignatures-Radiation-Mars-Life in extreme environments. Astrobiology 12, 718-729.     

Ionizing radiation and life

Ionizing radiation is a ubiquitous feature of the Cosmos, from exogenous cosmic rays (CR) to the intrinsic mineral radioactivity of a habitable world, and its influences on the emergence and persistence of life are wide-ranging and profound. Much attention has already been focused on the deleterious effects of ionizing radiation on organisms and the complex molecules of life, but ionizing radiation also performs many crucial functions in the generation of habitable planetary environments and the origins of life. This review surveys the role of CR and mineral radioactivity in star formation, generation of biogenic elements, and the synthesis of organic molecules and driving of prebiotic chemistry. Another major theme is the multiple layers of shielding of planetary surfaces from the flux of cosmic radiation and the various effects on a biosphere of violent but rare astrophysical events such as supernovae and gamma-ray bursts. The influences of CR can also be duplicitous, such as limiting the survival of surface life on Mars while potentially supporting a subsurface biosphere in the ocean of Europa. This review highlights the common thread that ionizing radiation forms between the disparate component disciplines of astrobiology.     

Bacterial growth at the high concentrations of magnesium sulfate found in martian soils

The martian surface environment exhibits extremes of salinity, temperature, desiccation, and radiation that would make it difficult for terrestrial microbes to survive. Recent evidence suggests that martian soils contain high concentrations of MgSO? minerals. Through warming of the soils, meltwater derived from subterranean ice-rich regolith may exist for an extended period of time and thus allow the propagation of terrestrial microbes and create significant bioburden at the near surface of Mars. The current report demonstrates that halotolerant bacteria from the Great Salt Plains (GSP) of Oklahoma are capable of growing at high concentrations of MgSO? in the form of 2 M solutions of epsomite. The epsotolerance of isolates in the GSP bacterial collection was determined, with 35% growing at 2 M MgSO?. There was a complex physiological response to mixtures of MgSO? and NaCl coupled with other environmental stressors. Growth also was measured at 1 M concentrations of other magnesium and sulfate salts. The complex responses may be partially explained by the pattern of chaotropicity observed for high-salt solutions as measured by agar gelation temperature. Select isolates could grow at the high salt concentrations and low temperatures found on Mars. Survival during repetitive freeze-thaw or drying-rewetting cycles was used as other measures of potential success on the martian surface. Our results indicate that terrestrial microbes might survive under the high-salt, low-temperature, anaerobic conditions on Mars and present significant potential for forward contamination. Stringent planetary protection requirements are needed for future life-detection missions to Mars.     

行星际空间环境对探测器可靠性影响分析

空间环境是影响航天器可靠性的重要因素。与地球轨道航天器相比,行星际探测任务可能会遭受更加恶劣的空间环境,例如极端温度环境,辐射环境,腐蚀性大气环境、宇宙尘等,再加上行星际任务寿命长,采用先进的器件和材料,空间环境对行星际探测器的可靠性构成严重的威胁,直接关系到探测目标能否实现。因此考虑空间环境对行星际探测器的影响,开展相关的预先研究无论是对于制定行星际空间探测计划,还是搭载仪器的设计都具有非常重要的意义。文章分析了极端温度、辐射环境和行星表面综合环境对探测器的影响,并对开展相关研究提出了建议。     

Sunspot dynamics are reflected in human physiology and pathophysiology

Periodic episodes of increased sunspot activity (solar electromagnetic storms) occur with 10-11 and 5-6 year periodicities and may be associated with measurable biological events. We investigated whether this sunspot periodicity characterized the incidence of Pap smear-determined cervical epithelial histopathologies and human physiologic functions. From January 1983 through December 2003, monthly averages were obtained for solar flux and sunspot numbers; six infectious, premalignant and malignant changes in the cervical epithelium from 1,182,421 consecutive, serially independent, screening Pap smears (59°9″N, 4°29″E); and six human physiologic functions of a healthy man (oral temperature, pulse, systolic and diastolic blood pressure, respiration, and peak expiratory flow), which were measured ～5 times daily during ～34,500 self-measurement sessions (44°56″N, 93°8″W). After determining that sunspot numbers and solar flux, which were not annually rhythmic, occurred with a prominent 10-year and a less-prominent 5.75-year periodicity during this 21-year study span, each biological data set was analyzed with the same curve-fitting procedures. All six annually rhythmic Pap smear-detected infectious, premalignant and malignant cervical epithelial pathologies showed strong 10-year and weaker 5.75-year cycles, as did all six self-measured, annually rhythmic, physiologic functions. The phases (maxima) for the six histopathologic findings and five of six physiologic measurements were very near, or within, the first two quarters following the 10-year solar maxima. These findings add to the growing evidence that solar magnetic storm periodicities are mirrored by cyclic phase-locked rhythms of similar period length or lengths in human physiology and pathophysiology.     

Critical issues in connection with human planetary missions: protection of and from the environment.

Activities associated with human missions to the Moon or to Mars will interact with the environment in two reciprocal ways: (i) the mission needs to be protected from the natural environmental elements that can be harmful to human health, the equipment or to their operations: (ii) the specific natural environment of the Moon or Mars should be protected so that it retains its value for scientific and other purposes. The following environmental elements need to be considered in order to protect humans and the equipment on the planetary surface: (i) cosmic ionizing radiation, (ii) solar particle events; (iii) solar ultraviolet radiation; (iv) reduced gravity; (v) thin atmosphere; (vi) extremes in temperatures and their fluctuations; (vii) surface dust; (viii) impacts by meteorites and micrometeorites. In order to protect the planetary environment. the requirements for planetary protection as adopted by COSPAR for lander missions need to be revised in view of human presence on the planet. Landers carrying equipment for exobiological investigations require special consideration to reduce contamination by terrestrial microorganisms and organic matter to the Greatest feasible extent. Records of human activities on the planet's surface should be maintained in sufficient detail that future scientific experimenters can determine whether environmental modifications have resulted from explorations. Grant numbers: 14056/99/NL/PA.     

基于液体金属的接触PIM抑制方法和实验验证

接触非线性是射频设备和电路中产生无源互调（passive intermodulation，PIM）信号的重要原因之一。基于对接触结点射频等效电路的分析，提出了一种基于共晶镓铟合金（eutectic gallium-indium，EGaIn）的PIM抑制方法。EGaIn不同于传统的焊锡，它可以在室温下实现接触结位置上的稳定、可重构连接。首先分析了射频（radio frequency，RF）下的金属接触结的电路模型。其次设计并实现了两个包含不同金属接触结构且工作在2.6GHz的平面倒F天线（planar inverted F antenna，PIFA）。最后比较了EGaIn焊接前后PIFA的互调水平，其中对PIM指标的最大抑制程度为35dB。验证了EGaIn可以在室温下对PIFA的接触结点进行可重构焊接，这为电连接中的非线性抑制提供了一种参考方法。     

高温SiC器件及其集成电路在空间电子领域应用展望

金星、火星等行星空间探索对航天器电子学系统耐高温、抗辐照、抗腐蚀性等提出了严峻挑战。极低温电子学和高温电子学是近年来深空探索中的强烈需求。文章主要阐述高温SiC集成电路的起源,数字、模拟和功率IC的发展历程和研究现状。重点综述分析了高温SiC集成电路设计方法和流片验证的两条技术途径：首先,基于多层外延制造工艺的BJT器件单元及其双极集成电路;其次,基于离子注入掺杂工艺的互补单元及其CMOS集成电路。在此基础上还进一步介绍了高温SiC传感芯片、BCD功率IC及功率模块的应用可靠性验证。目前国际研究现状展示了SiC BJT和CMOS IC研制中大学学术界和半导体企业界的协同创新格局。最后展望了其在深空探索中的潜在应用及其面临的挑战性。本综述对国内研制空间环境用宽禁带半导体SiC高温集成电路及其电子学系统具有借鉴价值。     

Implications of subzero metabolic activity on long-term microbial survival in terrestrial and extraterrestrial permafrost

The survival of microorganisms over extended time frames in frozen subsurface environments may be limited by chemical (i.e., via hydrolysis and oxidation) and ionizing radiation-induced damage to chromosomal DNA. In an effort to improve estimates for the survival of bacteria in icy terrestrial and extraterrestrial environments, we determined rates of macromolecular synthesis at temperatures down to -15°C in bacteria isolated from Siberian permafrost (Psychrobacter cryohalolentis K5 and P. arcticus 273-4) and the sensitivity of P. cryohalolentis to ionizing radiation. Based on experiments conducted over ≈400 days at -15°C, the rates of protein and DNA synthesis in P. cryohalolentis were <1 to 16 proteins cell(-1) d(-1) and 83 to 150 base pairs (bp) cell(-1) d(-1), respectively; P. arcticus synthesized DNA at rates of 20 to 1625?bp cell(-1) d(-1) at -15°C under the conditions tested. The dose of ionizing radiation at which 37% of the cells survive (D(37)) of frozen suspensions of P. cryohalolentis was 136?Gy, which was ～2-fold higher (71?Gy) than identical samples exposed as liquid suspensions. Laboratory measurements of [(3)H]thymidine incorporation demonstrate the physiological potential for DNA metabolism at -15°C and suggest a sufficient activity is possible to offset chromosomal damage incurred in near-subsurface terrestrial and martian permafrost. Thus, our data imply that the longevity of microorganisms actively metabolizing within permafrost environments is not constrained by chromosomal DNA damage resulting from ionizing radiation or entropic degradation over geological time.     

Suppression of the water ice and snow albedo feedback on planets orbiting red dwarf stars and the subsequent widening of the habitable zone

M stars comprise 80% of main sequence stars, so their planetary systems provide the best chance for finding habitable planets, that is, those with surface liquid water. We have modeled the broadband albedo or reflectivity of water ice and snow for simulated planetary surfaces orbiting two observed red dwarf stars (or M stars), using spectrally resolved data of Earth's cryosphere. The gradual reduction of the albedos of snow and ice at wavelengths greater than 1 μm, combined with M stars emitting a significant fraction of their radiation at these same longer wavelengths, means that the albedos of ice and snow on planets orbiting M stars are much lower than their values on Earth. Our results imply that the ice/snow albedo climate feedback is significantly weaker for planets orbiting M stars than for planets orbiting G-type stars such as the Sun. In addition, planets with significant ice and snow cover will have significantly higher surface temperatures for a given stellar flux if the spectral variation of cryospheric albedo is considered, which in turn implies that the outer edge of the habitable zone around M stars may be 10-30% farther away from the parent star than previously thought.     

AIT阶段微生物灭菌技术在行星保护任务中的应用与发展

行星保护是每一个开展深空探测活动的国家都应遵守的国际化行为。基于我国深空探测任务中行星保护相关的微生物控制需求,文章首先分析了深空探测器在AIT（总装、集成和测试）阶段负载的微生物主要种类和来源,之后综述NASA和ESA采用的干热灭菌（DHMR）、气相过氧化氢（VHP）等微生物灭菌技术在行星保护任务中的应用与研究现状,最后对加快微生物灭菌技术研究以支持我国未来的行星探测任务提出建议。     

The BOSS and BIOMEX space experiments on the EXPOSE-R2 mission: Endurance of the desert cyanobacterium Chroococcidiopsis under simulated space vacuum,Martian atmosphere,UVC radiation and temperature extremes.

The proposed space experiments BOSS (Biofilm Organisms Surfing Space) and BIOMEX (BIOlogy and Mars experiment) will take place on the space exposure facility EXPOSE-R2 on the International Space Station (ISS), which is set to be launched in 2014. In BOSS the hypothesis to be tested is that microorganisms grown as biofilms, hence embedded in self-produced extracellular polymeric substances, are more tolerant to space and Martian conditions compared to their planktonic counterparts. Various microbial biofilms have been developed including those obtained from the cyanobacterium Chroococcidiopsis isolated from hot and cold deserts. The prime objective of BIOMEX is to evaluate to what extent biomolecules are resistant to, and can maintain their stability under, space and Mars-like conditions; therefore a variety of pigments and cell components are under investigation to establish a biosignature data base; e.g. a Raman spectral library to be used for extraterrestrial life biosignatures. The secondary objective of BIOMEX is to investigate the endurance of extremophiles, focusing on their interactions with Lunar and Martian mineral analogues. Ground-based studies are currently being carried out in the framework of EVTs (Experiment Verification Tests) by exposing selected organisms to space and Martian simulations. Results on a desert strain of Chroococcidiopsis obtained from the first set of EVT, e.g. space vacuum, Mars atmosphere, UVC radiation, temperature cycles and extremes, suggested that dried biofilms exhibited an enhanced survival compared to planktonic lifestyle. Moreover the protection provided by a Martian mineral analogue (S-MRS) to the sub-cellular integrities of Chroococcidiopsis against UVC radiation supports the endurance of this cyanobacterium under extraterrestrial conditions and its relevance in the development of life detection strategies.     

连续波激光推力器稳定工作条件的解析计算模型

连续波激光推力器工作需要稳定的等离子体以吸收激光。为得到推力器中等离子体稳定维持的参数条件,根据吸收室内绕等离子体区域流动的流场特征,考虑高温状态下工质的热物理性质、激光吸收和辐射效应,建立了推力器吸收室内的能量平衡模型,该模型包含入射激光功率、推力室压强、等离子体区核心温度以及工质质量流量等参数。计算表明,该模型所得稳定质量流量随压力、激光功率的变化趋势与实验结果一致,并解释了稳定质量流量区间存在的原因。     

Issues in planetary protection: policy, protocol and implementation

Planetary protection is NASA's term for the practice of protecting solar system bodies from Earth life while protecting Earth from life that may be brought back from other solar system bodies. Spacefaring nations will soon begin retrieving samples from Mars and other solar system bodies. For these samples, planetary protection is in order, and measures are already in place to prevent the forward contamination of Mars and other bodies by Earth microbes and the backward contamination of Earth by possible extraterrestrial life. A major goal of planetary protection controls on forward contamination is to preserve the planetary record of natural processes by preventing human-caused microbial introductions.     

Microbial survival rates of Escherichia coli and Deinococcus radiodurans under low temperature, low pressure, and UV-Irradiation conditions, and their relevance to possible Martian life

Viability rates were determined for microbial populations of Escherichia coli and Deinococcus radiodurans under the environmental stresses of low temperature (-35 degrees C), low-pressure conditions (83.3 kPa), and ultraviolet (UV) irradiation (37 W/m(2)). During the stress tests the organisms were suspended in saltwater soil and freshwater soil media, at variable burial depths, and in seawater. Microbial populations of both organisms were most susceptible to dehydration stress associated with low-pressure conditions, and to UV irradiation. However, suspension in a liquid water medium and burial at larger depths (5 cm) improved survival rates markedly. Our results indicate that planetary surfaces that possess little to no atmosphere and have low water availability do not constitute a favorable environment for terrestrial microorganisms.     

无载荷舱航空相机的热控设计与试验验证

为保证航空相机在平流层空间热环境下稳定运行,对无载荷舱的航空相机进行热设计和验证.基于20 km海拔高度下相机外部的对流及辐射环境特点,采用一维对称平板测试法确定选取多层隔热组件作为相机蒙皮材料,选取聚氨酯泡沫作为隔热填充材料.对载荷相机进行热控系统设计,并利用有限元软件分析极端工况下相机的温度场分布,最终设计热控总功...