Biological dosimetry to determine the UV radiation climate inside the MIR station and its role in vitamin D biosynthesis

The vitamin D synthesis in the human skin, is absolutely dependent on UVB radiation. Natural UVB from sunlight is normally absent in the closed environment of a space station like MIR. Therefore it was necessary to investigate the UV radiation climate inside the station resulting from different lamps as well as from occasional solar irradiation behind a UV-transparent quartz window. Biofilms, biologically weighting and integrating UV dosimeters successfully applied on Earth (e.g. in Antarctica) and in space (D-2, Biopan I) were used to determine the biological effectiveness of the UV radiation climate at different locations in the space station. Biofilms were also used to determine the personal UV dose of an individual cosmonaut. These UV data were correlated with the concentration of vitamin D in the cosmonaut's blood and the dietary vitamin D intake. The results showed that the UV radiation climate inside the Mir station is not sufficient for an adequate supply of vitamin D, which should therefore be secured either by vitamin D supplementat and/or by the regular exposure to special UV lamps like those in sun-beds. The use of natural solar UV radiation through the quartz window for ‘sunbathing’ is dangerous and should be avoided even for short exposure periods.     

Monitoring of environmental UV radiation by biological dosimeters.

As a consequence of the stratospheric ozone layer depletion biological systems can be damaged due to increased UV-B radiation. The aim of biological dosimetry is to establish a quantitative basis for the risk assessment of the biosphere. DNA is the most important target molecule of biological systems having special sensitivity against short wavelength components of the environmental radiation. Biological dosimeters are usually simple organisms, or components of them, modeling the cellular DNA. Phage T7 and polycrystalline uracil biological dosimeters have been developed and used in our laboratory for monitoring the environmental radiation in different radiation conditions (from the polar to equatorial regions). Comparisons with Robertson-Berger (RB) meter data, as well as with model calculation data weighted by the corresponding spectral sensitivities of the dosimeters are presented. Suggestion is given how to determine the trend of the increase in the biological risk due to ozone depletion.     

Biological monitoring of radiation exposure.

Complementary to physical dosimetry, biological dosimetry systems have been developed and applied which weight the different components of environmental radiation according to their biological efficacy. They generally give a record of the accumulated exposure of individuals with high sensitivity and specificity for the toxic agent under consideration. Basically three different types of biological detecting/ monitoring systems are available: (i) intrinsic biological dosimeters that record the individual radiation exposure (humans, plants, animals) in measurable units. For monitoring ionizing radiation exposure, in situ biomarkers for genetic (e.g. chromosomal aberrations in human lymphocytes, germ line minisatellite mutation rates) or metabolic changes in serum, plasma and blood (e.g. serum lipids, lipoproteins, lipid peroxides, melatonin, antibody titer) have been used. (ii) Extrinsic biological dosimeters/indicators that record the accumulated dose in biological model systems. Their application includes long-term monitoring of changes in environmental UV radiation and its biological implications as well as dosimetry of personal UV exposure. (iii) Biological detectors/biosensors for genotoxic substances and agents such as bacterial assays (e.g. Ames test, SOS-type test) that are highly sensitive to genotoxins with high specificity. They may be applicable for different aspects in environmental monitoring including the International Space Station.     

Biological space experiments for the simulation of Martian conditions: UV radiation and Martian soil analogues.

The survivability of resistant terrestrial microbes, bacterial spores of Bacillus subtilis, was investigated in the BIOPAN facility of the European Space Agency onboard of Russian Earth-orbiting FOTON satellites (BIOPAN I -III missions). The spores were exposed to different subsets of the extreme environmental parameters in space (vacuum, extraterrestrial solar UV, shielding by protecting materials like artificial meteorites). The results of the three space experiments confirmed the deleterious effects of extraterrestrial solar UV radiation which, in contrast to the UV radiation reaching the surface of the Earth, also contains the very energy-rich, short wavelength UVB and UVC radiation. Thin layers of clay, rock or meteorite material were shown to be only successful in UV-shielding, if they are in direct contact with the spores. On Mars the UV radiation climate is similar to that of the early Earth before the development of a protective ozone layer in the atmosphere by the appearance of the first aerobic photosynthetic bacteria. The interference of Martian soil components and the intense and nearly unfiltered Martian solar UV radiation with spores of B. subtilis will be tested with a new BIOPAN experiment, MARSTOX. Different types of Mars soil analogues will be used to determine on one hand their potential toxicity alone or in combination with solar UV (phototoxicity) and on the other hand their UV protection capability. Two sets of samples will be placed under different cut-off filters used to simulate the UV radiation climate of Mars and Earth. After exposure in space the survival of and mutation induction in the spores will be analyzed at the DLR, together with parallel samples from the corresponding ground control experiment performed in the laboratory. This experiment will provide new insights into the principal limits of life and its adaptation to environmental extremes on Earth or other planets which and will also have implications for the potential for the evolution and distribution of life.     

Survival of microorganisms in space protected by meteorite material: results of the experiment 'EXOBIOLOGIE' of the PERSEUS mission.

During the early evolution of life on Earth, before the formation of a protective ozone layer in the atmosphere, high intensities of solar UV radiation of short wavelengths could reach the surface of the Earth. Today the full spectrum of solar UV radiation is only experienced in space, where other important space parameters influence survival and genetic stability additionally, like vacuum, cosmic radiation, temperature extremes, microgravity. To reach a better understanding of the processes leading to the origin, evolution and distribution of life we have performed space experiments with microorganisms. The ability of resistant life forms like bacterial spores to survive high doses of extraterrestrial solar UV alone or in combination with other space parameters, e.g. vacuum, was investigated. Extraterrestrial solar UV was found to have a thousand times higher biological effectiveness than UV radiation filtered by stratospheric ozone concentrations found today on Earth. The protective effects of anorganic substances like artificial or real meteorites were determined on the MIR station. In the experiment EXOBIOLOGIE of the French PERSEUS mission (1999) it was found that very thin layers of anorganic material did not protect spores against the deleterious effects of energy-rich UV radiation in space to the expected amount, but that layers of UV radiation inactivated spores serve as a UV-shield by themselves, so that a hypothetical interplanetary transfer of life by the transport of microorganisms inside rocks through the solar system cannot be excluded, but requires the shielding of a substantial mass of anorganic substances.     

Monitoring of biologically effective UV irradiance at El Arenosillo (INTA), Andalucia, in Spain.

Biological UV (ultraviolet) dosimetry was applied using the biofilm-technique (DLR patent) to determine the UV levels weighted of biologically weighted UV radiation at the INTA Sounding Station of El Arenosillo at Huelva, Spain (37 degrees 06'N, 6 degrees 44'W, 50 m a s 1=above sea level) on 2 days in 1997 [correction of 1977] (April 1, and May 5). Exposure periods were calculated for clear sky days using a radiative transfer model for erythemal doses to reach 1.3 to 1.5 MED (minimal erythemal dose). Reliability of the radiative transfer model was demonstrated by the doses registered by a Yankee-UV biometer for the same exposure periods as used for the biosensor. This work presents the methodology employed (biofilm-technique utilized [correction of utiliced], calculation of exposing periods with radiative transfer model, etc) and the results obtained with the Yankee biometer and the biofilm. At noon, the ratio of biofilm measurements (Ieff, W/m2=biological effective irradiance, in W/m2) to the UV Biometer data (in MED/h) was 3-4.     

Space radiation dosimetry using bubble detectors.

Bubble detectors--a new development in radiation detection--has only recently been used for radiation measurements in space. One important characteristic of the bubble detector is that it operates on a phenomenon which bears considerable resemblance to biological response. Recent experimental results from irradiating bubble detectors with high-energy heavy ions point to the need to re-examine the methodology used for assessing space radiation and the relevance of conventional quantities such as dose equivalent for space dosimetry. It may be that biological hazard associated with the intensely ionizing events--associated with nuclear fragmentation but delivering relatively small dose equivalent--may be much more important than that associated with lightly ionizing events which comprise the bulk of the conventional radiation dose equivalent.     

Issues and problems for radiobiological research in space.

The uniqueness of the space radiation field creates specific problems in the evaluation of hazards to men and materials. Comprehensive measurements of all physical parameters are necessary but not sufficient. Particular attention has to be paid to variables like solar flares by applying fast-responding active dosimetry. The assessment of biological consequences poses even more problems. There are no human data for the kinds of particles seen in space and they will presumably never be available. The only reasonable approach is therefore to use the information obtained for other radiations and check their applicability for the space situation. This involves both the study of fundamental processes in ground experiments as well as their verification in space missions. Special emphasis has to be laid on the modification of radiation effects by flight-dynamic factors and microgravity. Radiation protection guidelines for space flights cannot simply be transformed from existent regulations designed for radiation workers on earth but have to be tailored to the specific situation in space.     

Prediction of clear-sky biologically effective erythematic radiation (EER) From global solar radiation (250–2800 nm) at Cairo,Egypt

The hourly and daily measured clear-sky global solar radiation (G) and biologically important effective erythematic radiation (EER) incident on a horizontal surface at Cairo, Egypt (latitude 30° 05′ N & Longitude 31° 15′ E), during the period from January 1995 to December 2005 are used in this paper. The relationship between daily integrated totals of EER and the daily totals of broadband global solar radiation (250–2800 nm) is established. The temporal variability of the percentage ratio of the total daily erythema to total daily broadband solar global irradiation (EER/G) is determined. The monthly and the seasonal averages of the extraterrestrial UVB solar radiation, Mesurad and estímated UVB solar radiation and clearness index K_tUVB of UVB radiation are discussed. The average monthly mean variation of slant ozone (Z) and UVB transmission (K_tUVB) at the present work are found. The two variables show an opposite seasonal behavior, and the average monthly of slant ozone column and UVB transmission values shows the relationship between them in a clearer way than those of daily values. The estimated values of UVB solar radiation a good agreement with the measured values of the UVB solar radiation, the difference between the estimated and measured values of UVB solar radiation varies from 1.2% to 2.8%. The effect of the annual cycles of solar zenith angle (SZA) and total column ozone (TCO) on the ratios (EER/G) are presented and the correction factors are determined for removal of the ozone cycle. The seasonal variability of EER/G is also discussed. The effect of the annual cycles of solar zenith angle (SZA) and total column ozone (TCO) on the ratios (EER/G) is presented and the correction factors are determined for removal of the ozone cycle.     

Capability for ozone high-precision retrieval on JEM/SMILES observation

We estimate the capability of ozone (O₃) retrieval with the Superconducting Submillimeter-Wave Limb-Emission Sounder (SMILES) instrument attached to the Exposed Facility of the Japanese Experiment Module (JEM) on the International Space Station (ISS). SMILES carries a 4-K mechanical refrigerator to cool superconducting devices in space. Since SMILES has high sensitivity thanks to the superconducting receiver, it is expected that SMILES has ability to retrieve O₃ profiles more precisely than the previous millimeter–submillimeter limb measurements from satellites.     

Biological UV dosimeters in simulated space conditions.

Polycrystalline uracil thin layers participate in the phage and uracil response (PUR) experiment, assigned to the biological dosimetry of the extraterrestrial solar radiation on the International Space Station (ISS). In ground based experiments (experiment verification tests), the following space parameters were simulated and studied: temperature, vacuum and short wavelength UV (UV-C, down to 200 nm) radiation. The closed uracil samples proved to be vacuum-tight for 7 days. In the tested temperature range (from -20 to +40 degrees C) the uracil samples are stable. The kinetic of dimer formation (dimerization) and reversion (monomerization) of uracil dimers due to short wavelength UV radiation was detected, the monomerization efficiency of the polychromatic deuterium lamp is higher than that of the germicidal lamp. A mathematical model describing the kinetic of monomerization-dimerization was constructed. Under the influence of UV radiation the dimerization-monomerization reactions occur simultaneously, thus the additivity law of the effect of the various wavelengths is not applicable.     

Radiobiology and photobiology on Earth and in space: points of encounter and protection considerations.

All radiations originate in space, and the spectrum of radiations reaching the troposphere is limited only because of their range and absorption by the ozone layer above the atmosphere. Ultraviolet-C and the very heavy ions are therefore produced on earth only artificially, by special lamps and in accelerators. The range of biological effects of the different UV radiations and low and high LET radiations have been studied extensively, yet only recently new facts such as the production of DNA strand breaks by long wave UV light were established, adding to the various points of encounter existing between ionizing and nonionizing radiations. There are some similarities in radiation products, and the resulting effects of insult by radiation on biological systems very often are similar, if not the same. A common phenomenon that exists in all healthy biological cells is the ability to repair damage to DNA and thus either survive or mutate, and although the specific mechanisms of repair are somewhat different, the end result is the same. Recently a mechanism of improved radioprotection was found to involve an effect of certain radioprotective compounds on DNA repair. It is suggested that improved, and nontoxic, modes of protection may be offered by employing such compounds as biological response modifiers and natural substances. Further research is needed and is under way.     

Quantification of biologically effective environmental UV irradiance.

To determine the impact of environmental UV radiation on human health and ecosystems demands monitoring systems that weight the spectral irradiance according to the biological responses under consideration. In general, there are three different approaches to quantify a biologically effective solar irradiance. (i) weighted spectroradiometry where the biologically weighted radiometric quantities are derived from spectral data by multiplication with an action spectrum of a relevant photobiological reaction, e.g. erythema, DNA damage, skin cancer, reduced productivity of terrestrial plants and aquatic foodweb, (ii) wavelength integrating chemical-based or physical dosimetric systems with spectral sensitivities similar to a biological response curve, and (iii) biological dosimeters that directly weight the incident UV components of sunlight in relation to the effectiveness of the different wavelengths and to interactions between them. Most biological dosimeters, such as bacteria, bacteriophages, or biomolecules, are based on the UV sensitivity of DNA. If precisely characterized, biological dosimeters are applicable as field and personal dosimeters.     

Spore dosimetry of solar UV radiation: applications to monitoring of daily irradiance and personal exposure.

Environmental UV radiation can be quantified using spore dosimetry, which measures the inactivation of repair-deficient Bacillus subtilis spores dried on a membrane filter. The system exhibits highly selective sensitivity to UV radiation, not being affected by various environmental adversities, such as high and low temperature and humidity. Biologically-effective dose rate and cumulative dose of ambient radiation are measurable under various conditions at various places on the earth, including tropical, temperate, and polar sites. Applications to monitor the exposure at the surface of organisms including humans and plants have also been advanced.     

Biological dosimetry in Russian and Italian astronauts.

Large uncertainties are associated with estimates of equivalent dose and cancer risk for crews of long-term space missions. Biological dosimetry in astronauts is emerging as a useful technique to compare predictions based on quality factors and risk coefficients with actual measurements of biological damage in-flight. In the present study, chromosomal aberrations were analyzed in one Italian and eight Russian cosmonauts following missions of different duration on the MIR and the international space station (ISS). We used the technique of fluorescence in situ hybridization (FISH) to visualize translocations in chromosomes 1 and 2. In some cases, an increase in chromosome damage was observed after flight, but no correlation could be found between chromosome damage and flight history, in terms of number of flights at the time of sampling, duration in space and extra-vehicular activity. Blood samples from one of the cosmonauts were exposed in vitro to 6 MeV X-rays both before and after the flight. An enhancement in radiosensitivity induced by the spaceflight was observed.     

The total ozone and UV solar radiation over Stara Zagora,Bulgaria

The results from direct ground-based solar UV irradiance measurements and the total ozone content (TOC) over Stara Zagora (42° 25′N, 25° 37′E), Bulgaria are presented. During the period 1999–2003 the TOC data show seasonal variations, typical for the middle latitudes – maximum in the spring and minimum in the autumn. The comparison between TOC ground-based data and Global Ozone Monitoring Experiment (GOME) satellite-borne ones shows a seasonal dependence of the differences between them.A strong negative relationship between the total ozone and the 305 nm wavelength irradiance was found. The dependence between the two variables is significant (r = −0.62 ± 0.18) at 98% confidence level.The direct sun UV doses for some specific biological effects (erythema and eyes) are obtained. The estimation of the radiation amplification factor RAF shows that the ozone reduction by 1% increases the erythemal dose by 2.3%. The eye-damaging doses are more influenced by the TOC changes and in this case RAF = −2.7%.The amount of these biological doses depended on the solar altitude over the horizon. This dependence was not so strong when the total ozone content in the atmosphere was lower.     

空间中子辐射及其测量技术

空间辐射环境包含大量高能带电粒子,其与航天器的结构材料相互作用会产生次级中子辐射,由于这些中子的平均品质因子是带电粒子的约4~5倍,因而对生物组织的剂量当量相对贡献更大。由于中子与物质的作用机制更为复杂,测量难度大,使得准确测量空间中子注量及其剂量当量贡献成为空间辐射剂量学中最具挑战性的工作之一。主要介绍了空间中子辐射的产生机制以及国外相关测量方法及其相关校准技术的研究状况。     

Changes in surface UV solar irradiance and ozone over the balkans during the eclipse of August 11, 1999

Intensive measurements of UV solar irradiance, total ozone and surface ozone were carried out during the solar eclipse of 11 August 1999 at Thessaloniki, Greece and Stara Zagora, Bulgaria, located very close to the footprint of the moon's shadow during the solar eclipse with the maximum coverage of the solar disk reaching about 90% and 96% respectively. It is shown that during the eclipse the diffuse component is reduced less compared to the decline of the direct solar irradiance at the shorter wavelengths. A 20-minute oscillation of erythemal UV-B solar irradiance was observed before and after the time of the eclipse maximum under clear skies, indicating a possible 20-minute fluctuation in total ozone presumably caused by the eclipse induced gravity waves. The surface ozone measurements at Thessaloniki display a decrease of around 10–15 ppbv during the solar eclipse. Similarly, ozone profile measurements with a lidar system indicate a decrease of ozone up to 2 km during the solar eclipse. The eclipse offered the opportunity to test our understanding of tropospheric ozone chemistry. The use of a chemical box model suggested that photochemistry can account for a significant portion of the observed surface ozone decrease.     

Solar UV radiation variations and their stratospheric and climatic effects

NIMBUS-7 SBUV measurements of the short-term solar UV variations caused by solar rotation and active-region evolution have determined the amplitude and wavelength dependence for the active-region component of solar UV variations. Intermediate-term variations lasting several months are associated with rounds of major new active regions. The UV flux stays near the peak value during the current solar cycle variation for more than two years and peaks about two years later than the sunspot number. NIMBUS-7 measurements have observed the concurrent stratospheric ozone variations caused by solar UV variations. There is now no doubt that solar UV variations are an important cause of short- and long-term stratospheric variations, but the strength of the coupling to the troposphere and to climate has not yet been proven.     

Observations of the ozone and nitrogen dioxide profiles in the TROICA-4 experiment

To investigate the vast area of Russia, a mobile scientific facility based in a railway carriage was developed. It is capable to perform continues measurements being coupled in a passenger train traveling along railroads. It was first equipped with a spectrometer for remote sensing of ozone and nitrogen dioxide in the atmosphere for the transcontinental observations into the chemistry of the atmosphere-4 expedition performed from February 18 to March 5, 1998. A twilight DOAS method, which was applied for retrieval of the nitrogen dioxide profiles basing on spectral measurements at the visible wavelengths (434–451 nm), is described in the paper. Main features of a new algorithm for retrieval of the ozone profile and total content using the differential structure of the UV spectrum (310–335 nm) are presented. The ozone and nitrogen dioxide contents are obtained and shortly validated against available alternative data.