Activity of the sympathetic-adrenomedullary system in rats after space flight on the COSMOS biosatellites

The indicators of adrenomedullary activity (catecholamine content (CA) and the activity of the catecholamine-synthesizing enzymes tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DBH)) were measured in the adrenal glands of rats living in a state of weightlessness for 18.5–19.5 days on board the biosatellites COSMOS 936 and COSMOS 1129. None of these indicators was significantly changed by space flight, neither in the group living in a state of weightlessness nor in the group living in a centrifuge on board the spacecraft and exposed to artificial gravity of 1 g (COSMOS 936). Animals exposed after space flight to repeated immobilization stress on Earth showed a significant decrease of adrenal adrenaline and an appreciable increase in adrenal TH activity compared to stressed animals which were not in space. These results suggest that a prolonged state of weightlessness during space flight does not by itself represent an intensive stressful stimulus for the adrenomedullary system but potentiates the response of cosmonauts to stress after return to Earth.     

Space flight, microgravity, stress, and immune responses.

Exposure of animals and humans to space flight conditions has resulted in numerous alterations in immunological parameters. Decreases in lymphocyte blastogenesis, cytokine production, and natural killer cell activity have all been reported after space flight. Alterations in leukocyte subset distribution have also been reported after flight of humans and animals in space. The relative contribution of microgravity conditions and stress to the observed results has not been established. Antiorthostatic, hypokinetic, hypodynamic, suspension of rodents and chronic head-down tilt bed-rest of humans have been used to model effects of microgravity on immune responses. After use of these models, some effects of space flight on immune responses, such as decreases in cytokine function, were observed, but others, such as alterations in leukocyte subset distribution, were not observed. These results suggest that stresses that occur during space flight could combine with microgravity conditions in inducing the changes seen in immune responses after space flight. The biological/biomedical significance of space flight induced changes in immune parameters remains to be established. Grant Numbers: NCC2-859, NAG2-933.     

Biological dosimetry in Russian and Italian astronauts.

Large uncertainties are associated with estimates of equivalent dose and cancer risk for crews of long-term space missions. Biological dosimetry in astronauts is emerging as a useful technique to compare predictions based on quality factors and risk coefficients with actual measurements of biological damage in-flight. In the present study, chromosomal aberrations were analyzed in one Italian and eight Russian cosmonauts following missions of different duration on the MIR and the international space station (ISS). We used the technique of fluorescence in situ hybridization (FISH) to visualize translocations in chromosomes 1 and 2. In some cases, an increase in chromosome damage was observed after flight, but no correlation could be found between chromosome damage and flight history, in terms of number of flights at the time of sampling, duration in space and extra-vehicular activity. Blood samples from one of the cosmonauts were exposed in vitro to 6 MeV X-rays both before and after the flight. An enhancement in radiosensitivity induced by the spaceflight was observed.     

Changes of deoxyribonucleoprotein in the spleen, thymus and liver of rats exposed to weightlessness and artificial gravity aboard the Cosmos biosatellites

Changes of deoxyribonucleoprotein in the spleen, thymus and liver of rats exposed to wegithlessness or artifical gravity on board biosatellites Cosmos 782 and Cosmos 936 after 20 days of flight were investigated. The level of polydeoxyribonucleotides in the spleen and thymus of rats exposed during the flight to weightlessness increased 4 – 11 hours after landing, suggesting breakdown of a part of the deoxyribonucleoprotein present. The use of artifical gravity prevented this breakdown in the thymus but not in the spleen. The breakdown was accompanied in the majority of cases by a decrease in teh deoxyribonucleoprotein content. We believe the breakdown of deoxyribonucleoprotein is due to a nonspecific stress reaction to the change from the weightless state to that of terrestrial gravity during landing. The polydeoxyribonucleotide level and amount of deoxyribonucleoprotein in the majority of cases returned to normal values during the 25 days of readaptation. No substantial change of deoxyribonucleoprotein was found in the liver. The different findings in the three organs are due to the fact that breakdown of deoxyribonucleoprotein takes place in sensitive cells underlying pycnosis. These cells are found in the spleen and thymus, but not in the liver.     

Accumulation of tumor suppressor p53 in rat muscle after a space flight.

Tumor suppressor p53 functions as a cell cycle checkpoint under stressful conditions. Early studies have shown that genotoxic stress activates p53 pathway. Recently, many kinds of non-genotoxic stress such as heat shock, cold shock, and low pH also have been found to activate p53 pathway. The effects on living organism remains to be explored. Here, we show that an 18-day space flight induced a 3.6 fold accumulation of p53 in rat skeletal muscle. This results suggests that the p53 pathway plays a role in safeguarding genomic stability against the stressful space environments and supports our previous observation of p53 accumulation in rat skin after a space flight.     

Indo-German low-latitude project deos: plasma bubbles in the post sunset and nighttime sector

Two spread-F flights were performed under equinox conditions in April and September 1998 from the Indian low-latitude station SHAR. Both flights detected plasma bubbles confined to a narrow longitudinal extent. Plasma parameters are measured under two different conditions in the post-sunset ionosphere; when the F-layer is moving upward (19:21 IST launch) and when the F-layer is stationary (20:41 IST launch). Low altitude bubbles during upwelling of the F-region in the prereversal current enhancement phase are characterized by turbulent, non Maxwellian regions. Transitional scale waves with k=−3.6 are observed in the bubbles. This flight also detects strong sharp E-layers during downleg and an intermediate layer at 170km. Nighttime bubbles during almost stationary F-layer conditions show turbulent, Maxwellian features with moderate cooling compared to the undisturbed environment. Transitional scale waves in the bubble region show a spectral index k=−3.1. The spectral power is reduced by around 20dB compared to the sunset flight.     

Effects of space flight and IGF-1 on immune function

We tested the hypothesis that insulin-like growth factor-1 (IGF-1) would ameliorate space flight-induced effects on the immune system. Twelve male, Sprague-Dawley rats, surgically implanted with mini osmotic pumps, were subjected to space flight for 10 days on STS-77. Six rats received 10 mg/kg/day of IGF-1 and 6 rats received saline. Flight animals had a lymphocytopenia and granulocytosis which were reversed by IGF-1. Flight animals had significantly higher corticosterone levels than ground controls but IGF-1 did not impact this stress hormone. Therefore, the reversed granulocytosis did not correlate with serum corticosterone. Space flight and IGF-1 also combined to induce a monocytopenia that was not evident in ground control animals treated with IGF-1 or in animals subjected to space flight but given physiological saline. There was a significant increase in spleen weights in vivarium animals treated with IGF-1, however, this change did not occur in flight animals. We observed reduced agonist-induced lymph node cell proliferation by cells from flight animals compared to ground controls. The reduced proliferation was not augmented by IGF-1 treatment. There was enhanced secretion of TNF, IL-6 and NO by flight-animal peritoneal macrophages compared to vivarium controls, however, O₂ secretion was not affected. These data suggest that IGF-1 can ameliorate some of the effects of space flight but that space flight can also impact the normal response to IGF-1.     

Neurobiological problems in long-term deep space flights.

Future missions in space may involve long-term travel beyond the magnetic field of the Earth, subjecting astronauts to radiation hazards posed by solar flares and galactic cosmic rays, altered gravitation fields and physiological stress. Thus, it is critical to determine if there will be any reversible or irreversible, detrimental neurological effects from this prolonged exposure to space. A question of particular importance focuses on the long-term effects of the space environment on the central nervous system (CNS) neuroplasticity, with the potential acute and/or delayed effects that such perturbations might entail. Although the short-term effects of microgravity on neural control were studied on previous low earth orbit missions, the late consequences of stress in space, microgravity and space radiation have not been addressed sufficiently at the molecular, cellular and tissue levels. The possibility that space flight factors can interact influencing the neuroplastic response in the CNS looms critical issue not only to understand the ontogeny of the CNS and its functional integrity, but also, ultimately the performance of astronauts in extended space forays. The purpose of this paper is to review the neurobiological modifications that occur in the CNS exposed to the space environment, and its potential consequences for extended deep space flight.     

The effects of space flight on some rat liver enzymes regulating carbohydrate and lipid metabolism

We have examined, in the livers of rats carried aboard the Cosmos 936 biosatellite, the activities of about 30 enzymes concerned with carbohydrate and lipid metabolism. In addition to the enzyme studies, the levels of glycogen and of the individual fatty acids in hepatic lipids were determined. Livers from flight and ground control rats at recovery (R₀) and 25 days after recovery (R₂₅) were used for these analyses.

For all parameters measured, the most meaningful comparisons are those made between flight stationary (FS) and flight centrifuged (FC) animals at R₀. When these two groups of flight rats were compared at R₀, statistically significant decreases in the activity levels of glycogen phosphorylase, -glycerol phosphate acyl transferase, diglyceride acyl transferase, aconitase and 6-phosphogluconate dehydrogenase and an increase in the palmitoyl CoA desaturase were noted in the weightless group (FS). The significance of these findings was strengthened by the fact that all enzyme activities showing alterations at R₀ returned to normal 25 days postflight. When liver glycogen and total fatty acids of the two sets of flight animals were determined, significant differences that could be attributed to reduced gravity were observed. The weightless group (FS) at R₀ contained, on the average, more than twice the amount of glycogen than did the centrifuged controls (FC) and a remarkable shift in the ratio of palmitate to palmitoleate was noted. These metabolic alterations, both in enzyme levels and in hepatic constituents, appear to be characteristic of the weightless condition. Our data seem to justify the conclusion that centrifugation during flight is equivalent to terrestrial gravity.     

Leaf proteomic analysis of three rice heritable mutants after seed space flight

To explore the proteomic changes of heritable variant rice plants induced by space environment, three mutants were selected after seed space flight by comparing the phenotypes with their on-ground controls. R955 grew more tillers and became dwarf, 971-5 acquired higher grain yield and better stress resistance, 974-5 matured earlier. Leaf proteins were extracted during the tiller development and analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). More than 300 proteins were detected as reproducible Coomassie Brilliant Blue stained spots with pI values from around 4.0 to 7.0. Five proteins that changed significantly over the controls were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). The main functions of these proteins were photosynthesis, stress defense and metabolism including RuBisCO activase, glycine rich RNA binding protein, peroxidase, triosephosphate isomerase and phosphoenolpyruvate carboxylase, which might be probably associated with the altered phenotypes. Quantitative analyses were also applied: less total protein spots and more down-regulated protein spots were detected in the mutants, indicating there might be a major loss of protein in heritable variant rice plants after seed space flight. These results may provide new insights to understand the biological effects of space environment to rice.     

The effect of artificial gravity on plasma and tissue lipids in rats: The Cosmos 936 experiment

Plasma and tissue lipids in male SPF Wistar rats flown for 18.5 days aboard the Cosmos 936 biosatellite were analyzed. One group of rats was subjected to artificial gravity by use of a centrifuge during the flight. An experiment simulating known space flight factors other than weightlessness was done on Earth. An increase of total cholesterol in plasma, of nonesterified fatty acids in plasma and brown adipose tissue, of triacylglycerols in plasma, liver, thymus and bone marrow was noted several hours after biosatellite landing. Smaller changes were observed in the terrestrial control experiment. With the exception of triacylglycerol accumulation in bone marrow, these increases disappeared 25 days after biosatellite landing. Exposing the rats aboard the biosatellite to artificial gravity was beneficial in the sense that such exposure inhibited the phospholipid and triacylglycerol increase in plasma and inhibited the increase of triacylglycerol in liver and especially in bone marrow.     

民机飞行载荷谱编制方法

探索了由同类机型的飞行实测载荷统计数据,推断新设计机型的飞行谱,进而编制其载荷谱的新途径.采用国外同类飞机的飞行实测载荷统计数据,依据类比法,分析和推断出某民机的飞行谱以及各飞行状态连续应力谱;在此基础上,根据民用运输机的TWIST编谱方法,编制了各飞行状态的突风和机动载荷谱,最终,随机生成了其飞行谱对应的5×5地-空-地载荷谱,从而验证了探索途径的可行性与有效性.采用该方法可以加快民机研制周期.     

Balloon film strain measurement

In order to understand the state of stress in scientific balloons, a need exists for the measurement of film deformation in flight. The results of a flight test program are reported where material strain was measured for the first time during the inflation, launch, ascent and float of a typical natural shape, zero pressure scientific balloon.     

The effect of microgravity on the development of plant protoplasts flown on Biokosmos 9.

An experiment using plant protoplasts has been accepted for the IML-1 Space Shuttle mission scheduled for 1991. Preparatory experiments have been performed using both fast and slow rotating clinostats and in orbit to study the effect of simulated and real weightlessness on protoplast regeneration. Late access to the space vehicles before launch has required special attention since it is important to delay cell wall regeneration until the samples are in orbit. On a flight on Biokosmos 9 ("Kosmos-2044") in September 1989 some preliminary results were obtained. Compared to the ground control, the growth of both carrot and rapeseed protoplasts was decreased by 18% and 44% respectively, after 14 days in orbit. The results also indicated that there is less cell wall regeneration under micro-g conditions. Compared to the ground controls the production of cellulose in rapeseed and carrot flight samples was only 46% and 29% respectively. The production of hemicellulose in the flight samples was 63% and 67% respectively of that of the ground controls. In both cases all samples reached the stage of callus development. The peroxidase activity was also found to be lower in the flight samples than in the ground controls, and the number of different isoenzymes was decreased in the flight samples. In general, the regeneration processes were retarded in the flight samples with respect to the ground controls. From a simulation experiment for IML-1 performed in January 1990 at ESTEC, Holland, regenerated plants have been obtained. These results are discussed and compared to the results obtained on Biokosmos 9. Protoplast regeneration did not develop beyond the callus stage in either the flight or the ground control samples from the Biokosmos 9 experiment.     

可靠性试验中通风冷却剖面的探讨

结合国家军用标准和飞机环控系统的特点对机载电子设备实际使用中的通风冷却条件进行了研究,结合产品的寿命剖面、任务剖面,针对不同任务的环境条件、飞行高度和飞行马赫数等条件,对产品在可靠性试验中通风冷却应力的施加进行了探索性研究,对现行标准中存在的问题进行了对比研究,阐述了在可靠性试验过程中如何模拟其实际使用中的通风冷却条件,制定出合理、可行的通风冷却试验剖面(包括通风气体流量、温度、湿度和洁净度等参数的确定方法).以具体产品为例,通过对产品实际使用中的通风情况进行研究,得出了其可靠性试验中与温度剖面相对应的通风冷却剖面(包括通风气体的参数以及施加的时间).     

基于机械干涉理论估算无人机舵机电位计磨损可靠工作设计

基于机械产品强度与应力干涉理论,分析计算了无人机飞控系统重要组件舵机中的位置反馈电位计耐磨可靠度和可靠性系数,估算了不同可靠度情况下的舵机的可靠工作寿命,为无人机飞控系统设计和使用维修提供依据。     

深空再入返回飞行仿真及工程应用分析

深空高速再入对返回技术提出了许多新的挑战.采用传统弹道式返回,将使返回器面临残酷的气动热环境,同时需要解决落点偏差大的问题;采用新的预测制导返回技术需要解决返回过程的航迹规划、制导和姿态控制问题.根据球冠倒锥外形飞行器在大气层内再入飞行时的受力情况建立飞行器的动力学模型,然后对弹道式返回和预测制导返回两种飞行方案进行仿真,最后围绕返回飞行总体特性参数和返回方式的技术要求等进行工程应用分析,为深空返回的工程实践提供指导.     

Technology development for a long duration,mid-cloud level Venus balloon

This paper describes the results of ongoing technology development activities for a Venus spherical superpressure balloon capable of flying for long durations (30 days) in the middle cloud layer at an altitude of 55.5 km. Data is presented from a successful aerial deployment and inflation flight experiment on a 5.5 m diameter prototype balloon conducted at a 2.5 km altitude above the Earth. Although the balloon in that test was not released for free flight, all other steps in the deployment and inflation process were successfully executed. Experimental and computational results are also presented from an investigation of the stress concentration phenomenon at the junction of the metal end fitting and fabric end cap of the prototype Venus balloon. Good agreement was found between the simulation and experimental results and a stress concentration factor of 1.55 determined for this end cap design compared to the expectations of thin membrane theory. Finally, results are presented for a new, second-generation Venus balloon material utilizing Aclar™ film instead of Teflon. Optical property and sulfuric acid tolerance data are presented for this material based on laboratory testing of samples.     

Developments in balloon stress analysis

The use of large plastic balloons as a research tool has increased dramatically since the developmental work of the early 1950's. The continuing demand by the scientific community for higher float altitudes, heavier payloads, and longer flight durations has severely challenged current design and analysis procedures. Previous simplifying assumptions concerning the balloon shape and stress must be reassessed in order to develop better analytical design and stress analysis procedures. A brief history of balloon stress analysis procedures and accompanying assumptions are presented. The limitations of old methods and recent improvements by Smalley, Alexander, Rand, and others are examined and compared. Finite difference and finite element techniques offer promise for more accuracy with fewer over-simplifying assumptions. Available methods are examined for potential use in various stress analysis requirements.     

CSCW在空中交通管理中的应用

为了提高空中交通管理中航空器放行协作的工作效率,减少协作中的差错,利用计算机支持协作工作(CSCW,Computer Supported Cooperative Work)的理论和方法,设计实现了一种通用性强、适用性好的CSCW航空器放行协作系统.实际应用表明,该系统大大提高了航空器放行协作的工作效率,减轻了工作人员的工作负担,减少了错误的发生,具有很强的实用价值.