Dose equivalent, absorbed dose and charge spectrum investigation in low Earth orbit.

Particle intensity, dose equivalent and absorbed dose have been measured on board the space shuttle Endeavour during STS-108 in December 2001 by Dublin Institute for Advanced Studies (DIAS). The dose estimates are based on very accurate measurements of recoils produced in CR-39 by cosmic ray primary and secondary protons and heavier nuclei and by secondary neutrons. The corresponding LET spectra were used to determine dose equivalent and absorbed dose values. Estimates of the total flux of Z > or = 2 nuclei have been undertaken and a preliminary charge spectrum was measured. Some comparisons are made with preliminary data obtained on STS-105 (ISS Expedition) and other missions using CR-39 detectors.     

On the practical exploitation of perturbative effects in low Earth orbit for space debris mitigation

This paper presents the results of a numerical evaluation of the natural lifetime reduction in low Earth orbit, due to dynamical perturbations. The study considers two values for the area-to-mass ratio, a nominal ratio which resembles a typical value of spacecraft in orbit today, and an enhanced ratio which covers the surface augmentation. The results were obtained with two orbit propagators, one of a semi-analytical nature and the second one using non-averaged equations of motion. The simulations for both propagators were set up similarly to allow comparison. They both use the solar radiation pressure and the secular terms of the geopotential ($J_2,J_4$ and $J_6$). The atmospheric drag was turned on and off in both propagators to alternatively study the eccentricity build up and the residual lifetime. The non-averaging case also covers a validation with the full 6?×?6 geopotential. The results confirm the findings in previous publications, that is, the possibility for de-orbiting from altitudes above the residual atmosphere if a solar sail is deployed at the end-of-life, due to the combined effect of solar radiation pressure and the oblateness of the Earth. At near polar inclinations, shadowing effects can be exploited to the same end. The results obtained with the full, non-averaging propagator revealed additional de-orbiting corridors associated with solar radiation pressure which were not found by previous work on space debris mitigation. The results of both tools are compared for specific initial conditions. For nominal values of area-to-mass ratio, instead, it is confirmed that this resonance effect is negligible.The paper then puts the findings in the perspective of the current satellite catalogue. It identifies space missions which are currently close to a resonance corridor and shows the orbit evolution within the resonances with a significantly shorter residual orbital lifetime. The paper finishes with a discussion on the exploitation of these effects with regards to the long-term simulation of the space debris environment and a flux and collision probability comparison.     

Effects of orbit progression on the radiation exposures from solar proton fluxes in low Earth orbit under geomagnetic storm conditions.

The present study examines the effects of orbit progression on the exposures within a Space Station Freedom module in a 51.6-degree inclined orbit at 450 km. The storm evolution is modeled after the November 1960 event, and the solar proton flux evolution is taken from the August 1972 solar proton event. The effects of a strong magnetic shock, such as was observed during the October 1989 event, is also modeled. The statistics on hourly average storm fields for the last forty years reveal that the largest geomagnetic storms approach a Dst value of -500 nanotesla at the storm peak. Similarly, one of the largest satellite-measured proton flux (> 10 MeV) for space exposures is the event of August 1972. The effects of orbit progression (advance of the line of nodes) is examined for the above conditions to study the variation of exposures under differing times of occurrence of the solar proton peak intensity, attainment of geomagnetic storm maximum, and the location of the line of nodes of the last geomagnetically protected orbit. The impact of the inherent inhomogeneity of the space station module is examined as a limiting factor on exposure with regard to the need of additional parasitic shielding.     

Intracellular molecular distributions in spacecraft experiments in orbit around Earth

It is possible that the nucleolous inside the cell plays the role of a “gravity receptor”. Furthermore, cells up to 10 μm in diameter can demonstrate some effect due to the redistribution of mitochondria or nucleolous. Effects of gravity should be present in various cell systems where larger objects such as the ribosomes move from cell to cell. In this paper we study the effects of gravity on cells. In particular, we examine the resulting intracellular molecular distribution due to Brownian motion and the ordered distribution of molecules under the action of gravity, where n₀ is the number per unit volume at certain level, and n is the number per unit volume above that level. This is an experiment that takes place at a certain orbital altitude in a spacecraft in orbit around Earth, where the acceleration due to the central field is corrected for the oblateness and also the rotation of the Earth. We found that equatorial circular and elliptical orbits have the highest n/n₀ ratios. This experiment takes place in circular and elliptical orbits, with eccentricities e = 0, 0.1 and involves a bacterial cell at an orbital altitude of 300 km. We found that n/n₀ = 1.00299 and 1.0037 respectively, which is still a 0.6–0.7 % higher than n/n₀ = 0.0996685 calculated on the surface of the Earth. Examining mitochondria in similar orbital experiments we found that equatorial orbits result to higher n/n₀ ratios. In particular, we found that n/n₀ = 8.38119, where an elliptical orbit of eccentricity e = 0.1 results to n/n₀ = 13.8525. Both are high above 100%, signifying the importance of Brownian motion over gravity. Our results are of interest to biomedical applications. Molecular concentrations are important for various processes such as the embryogenesis, positional homeostasis and its relation to cell energy expenditure, cell torque, cell deformation, and more. These results indicate that statistical molecular distributions play an important role for the recognition of a particular environment by the cell, in biological space experiment to come.     

Impact of orbit modeling on DORIS station position and Earth rotation estimates

The high precision of estimated station coordinates and Earth rotation parameters (ERP) obtained from satellite geodetic techniques is based on the precise determination of the satellite orbit. This paper focuses on the analysis of the impact of different orbit parameterizations on the accuracy of station coordinates and the ERPs derived from DORIS observations. In a series of experiments the DORIS data from the complete year 2011 were processed with different orbit model settings. First, the impact of precise modeling of the non-conservative forces on geodetic parameters was compared with results obtained with an empirical-stochastic modeling approach. Second, the temporal spacing of drag scaling parameters was tested. Third, the impact of estimating once-per-revolution harmonic accelerations in cross-track direction was analyzed. And fourth, two different approaches for solar radiation pressure (SRP) handling were compared, namely adjusting SRP scaling parameter or fixing it on pre-defined values.     

Prebiotic synthesis of nucleotides at the Earth orbit in presence of Lunar soil.

Modern studies now favor the fact that extraterrestrial organic molecules served as an important source of biological important substances on the primitive Earth. It is presumed that these space-made organic molecules could be transported safely to the Earth surface being associated with mineral grains. It is important to test whether nucleotides synthesized in Earth orbit could be protected by lunar surface regolite. The phosphorylation of adenosine, uridine and thymidine has been studied with respect of their further transformations and degradation in presence of mineral bed. After retrieval, HPLC analysis is used to identify all the mononucleotides of certain nucleosides. It has been shown, that exposure of the investigated nucleosides as dry films in space conditions in the presence of Lunar soil increases the yield of synthesized nucleotides in 1.1-3.0 times as compared with the exposure of the same samples in absence of Lunar soil. To identify and evaluate the principal source of energy in open space responsible for nucleotide synthesis reaction laboratory experiments were performed. It has been shown, that vacuum ultra violet (VUV 145 nm) radiation promotes nucleotide synthesis more effectively than ultra violet (UV 254 nm) while the presence of Lunar soil increases reaction yield in 1.5-2.0 times. Formation of 5'-mononucleotides seemed to be the most effective reaction both in flight and in laboratory experiments. Protective action of lunar soil on synthesized nucleotides against UV radiation has been shown in open Space conditions.     

Differentiation of Dictyostelium discoideum vegetative cells into spores during Earth orbit in space.

We reported previously that emerged amoebae of Dictyostelium (D.) discoideum grew, aggregated and differentiated to fruiting bodies with normal morphology in space. Here, we investigated the effects of space radiation and/or microgravity on the number, viability, kinetics of germination, growth rate and mutation frequency of spores formed in space in a radiation-sensitive strain, gamma s13, and the parental strain, NC4. In gamma s13, there were hardly spores in the fruiting bodies formed in space. In NC4, we found a decrease in the number of spores, a delay in germination of the spores and delayed start of cell growth of the spores formed in space when compared to the ground control. However, the mutation frequency of the NC4 spores formed in space was similar to that of the ground control. We conclude that the depression of spore formation might be induced by microgravity and/or space radiation through the depression of some stage(s) of DNA repair during cell differentiation in the slime mold.     

DNA damage and repair in oncogenic transformation by heavy ion radiation.

Energetic heavy ions are present in galactic cosmic rays and solar particle events. One of the most important late effects in risk assessment is carcinogenesis. We have studied the carcinogenic effects of heavy ions at the cellular and molecular levels and have obtained quantitative data on dose-response curves and on the repair of oncogenic lesions for heavy particles with various charges and energies. Studies with repair inhibitors and restriction endonucleases indicated that for oncogenic transformation DNA is the primary target. Results from heavy ion experiments showed that the cross section increased with LET and reached a maximum value of about 0.02 micrometer2 at about 500 keV/micrometer. This limited size of cross section suggests that only a fraction of cellular genomic DNA is important in radiogenic transformation. Free radical scavengers, such as DMSO, do not give any effect on induction of oncogenic transformation by 600 MeV/u iron particles, suggesting most oncogenic damage induced by high-LET heavy ions is through direct action. Repair studies with stationary phase cells showed that the amount of reparable oncogenic lesions decreased with an increase of LET and that heavy ions with LET greater than 200 keV/micrometer produced only irreparable oncogenic damage. An enhancement effect for oncogenic transformation was observed in cells irradiated by low-dose-rate argon ions (400 MeV/u; 120 keV/micrometer). Chromosomal aberrations, such as translocation and deletion, but not sister chromatid exchange, are essential for heavy-ion-induced oncogenic transformation. The basic mechanism(s) of misrepair of DNA damage, which form oncogenic lesions, is unknown.     

Geomagnetic transmission disturbances and heavy-ion fluences observed in low Earth orbit during the solar energetic particle events of October 1989.

The large solar energetic particle (SEP) events and simultaneous large geomagnetic disturbances observed during October 1989 posed a significant, rapidly evolving space radiation hazard. Using data from the GOES-7, NOAA-10, IMP-8 and LDEF satellites, we determined the geomagnetic transmission, heavy ion fluences, mean Fe ionic charge state, and effective radiation hazard observed in low Earth orbit (LEO) for these SEPs. We modeled the geomagnetic transmission by tracing particles through the combination of the internal International Geomagnetic Reference Field (IGRF) and the Tsyganenko (1989) magnetospheric field models, extending the modeling to large geomagnetic disturbances. We used our results to assess the radiation hazard such very large SEP events would pose in the anticipated 52 degrees inclination space station orbit.     

Probability of cell hits in selected organs and tissues by high-LET particles at the ISS orbit.

The fluence of high-LET particles (HLP) with LET infinity H2O greater than 15 keV micrometers-1 in selected organs and tissues were measured with plastic nuclear track detectors using a life-size human phantom on the 9th Shuttle-Mir Mission (STS-91). The planar-track fluence of HLP during the 9.8-day mission ranged from 1.9 x 10(3) n cm-2 (bladder) to 5.1 x 10(3) n cm-2 (brain) by a factor of 2.7. Based on these data, a probability of HLP hits to a matured cell of each organ or tissue was roughly estimated for a 90-day ISS mission. In the calculation, all cells were assumed to be spheres with a geometric cross-sectional area of 500 micrometers2 and the cell-hit frequency from isotropic space radiation can be described by the Poisson-distribution function. As results, the probability of one or more than 1 hit to a single cell by HLP for 90 days ranged from 17% to 38%; that of two or more than 2 hits was estimated to be 1.3-8.2%.     

Energetic particle environment in near-Earth orbit.

The hazard of exposure to high doses of ionizing radiation is one of the primary concerns of extended manned space missions and a continuous threat for the numerous spacecraft in operation today. In the near-Earth environment the main sources of radiation are solar energetic particles (SEP), galactic cosmic rays (GCR), and geomagnetically trapped particles, predominantly protons and electrons. The intensity of the SEP and GCR source depends primarily on the phase of the solar cycle. Due to the shielding effect of the Earth's magnetic field, the observed intensity of SEP and GCR particles in a near-Earth orbit will also depend on the orbital parameters altitude and inclination. The magnetospheric source strength depends also on these orbital parameters because they determine the frequency and location of radiation belt passes. In this paper an overview of the various sources of radiation in the near-Earth orbit will be given and first results obtained with the Solar, Anomalous, and Magnetospheric Particle Explorer (SAMPEX) will be discussed. SAMPEX was launched on 3 July 1992 into a near polar (inclination 82 degrees) low altitude (510 x 675 km) orbit. The SAMPEX payload contains four separate instruments of high sensitivity covering the energy range 0.5 to several hundred MeV/nucleon for ions and 0.4 to 30 MeV for electrons. This low altitude polar orbit with zenith-oriented instrumentation provides a new opportunity for a systematic study of the near-Earth energetic particle environment.     

The role of DNA repair on cell killing by charged particles.

It can be noted that it is not simple double strand breaks (dsb) but the non-reparable breaks that are associated with high biological effectiveness in the cell killing effect for high LET radiation. Here, we have examined the effectiveness of fast neutrons and low (initial energy = 12 MeV/u) or high (135 MeV/u) energy charged particles on cell death in 19 mammalian cell lines including radiosensitive mutants. Some of the radiosensitive lines were deficient in DNA dsb repair such as LX830, M10, V3, and L5178Y-S cells and showed lower values of relative biological effectiveness (RBE) for fast neutrons if compared with their parent cell lines. The other lines of human ataxia-telangiectasia fibroblasts, irs 1, irs 2, irs 3 and irs1SF cells, which were also radiosensitive but known as proficient in dsb repair, showed moderated RBEs. Dsb repair deficient mutants showed low RBE values for heavy ions. These experimental findings suggest that the DNA repair system does not play a major role against the attack of high linear energy transfer (LET) radiations. Therefore, we hypothesize that a main cause of cell death induced by high LET radiations is due to non-reparable dsb, which are produced at a higher rate compared to low LET radiations.     

Graviceptor development in jellyfish ephyrae in space and on Earth.

Graviceptor (rhopalium) development in Aurelia aurita ephyrae which developed on Earth and in space during the nine-day NASA SLS-1 mission was compared. The space-developed ephyrae made graviceptors which were morphologically similar to those of their ground-based controls. Rhopalia of both groups developed statocysts with statoliths, ocelli, ciliated mechanoreceptor cells, and immature touch-plates with one type of hair cell. The number of rhopalia formed per arm of ephyrae of both groups revealed no significant differences. The number of statoliths formed per rhopalium was statistically higher in ephyrae which were induced to form in space with iodine than in L(Launch)+8h controls. Statolith numbers were not significantly different between Earth-formed control ephyrae and those formed from polyps induced on Earth and then sent into space 24h and 48h later. Statolith loss from rhopalia was significantly enhanced in the space-maintained ephyrae in ASW as compared to their controls. Ephyrae formed through thyroxine treatment and those maintained in thyroxine in space had statolith numbers comparable to thyroxine-treated controls. Pulsing abnormalities seen in some space-developed ephyrae suggest that some space-formed ephyrae may have developed abnormal rhopalia because normal rhopalia development and function is necessary for normal pulsing.     

Analysis of the orbit lifetime of CubeSats in low Earth orbits including periodic variation in drag due to attitude motion

It is estimated that more than 22,300 human-made objects are in orbit around the Earth, with a total mass above 8,400,000 kg. Around 89% of these objects are non-operational and without control, which makes them to be considered orbital debris. These numbers consider only objects with dimensions larger than 10 cm. Besides those numbers, there are also about 2000 operational satellites in orbit nowadays. The space debris represents a hazard to operational satellites and to the space operations. A major concern is that this number is growing, due to new launches and particles generated by collisions. Another important point is that the development of CubeSats has increased exponentially in the last years, increasing the number of objects in space, mainly in the Low Earth Orbits (LEO). Due to the short operational time, CubeSats boost the debris population. One of the requirements for space debris mitigation in LEO is the limitation of the orbital lifetime of the satellites, which needs to be lower than 25 years. However, there are space debris with longer estimated decay time. In LEÓs, the influence of the atmospheric drag is the main orbital perturbation, and is used in maneuvers to increment the losses in the satellite orbital energy, to locate satellites in constellations and to accelerate the decay.The goal of the present research is to study the influence of aerodynamic rotational maneuver in the CubeSat?s orbital lifetime. The rotational axis is orthogonal to the orbital plane of the CubeSat, which generates variations in the ballistic coefficient along the trajectory. The maneuver is proposed to accelerate the decay and to mitigate orbital debris generated by non-operational CubeSats. The panel method is selected to determine the drag coefficient as a function of the flow incident angle and the spinning rate. The pressure distribution is integrated from the satellite faces at hypersonic rarefied flow to calculate the drag coefficient. The mathematical model considers the gravitational potential of the Earth and the deceleration due to drag. To analyze the effects of the rotation during the decay, multiple trajectories were propagated, comparing the results obtained assuming a constant drag coefficient with trajectories where the drag coefficient changes periodically. The initial perigees selected were lower than 400 km of altitude with eccentricities ranging from 0.00 to 0.02. Six values for the angular velocity were applied in the maneuver. The technique of rotating the spacecraft is an interesting solution to increase the orbit decay of a CubeSat without implementing additional de-orbit devices. Significant changes in the decay time are presented due to the increase of the mean drag coefficient calculated by the panel method, when the maneuver is applied, reducing the orbital lifetime, however the results are independent of the angular velocity of the satellite.     

The theoretical consideration of microgravity effects on a cell.

Mechanical processes and factors involved in gravireception of a plant cell qualitatively considered and their changes caused by microgravity and clinostat modeling conditions are discussed. It is supposed that the most of the cell microgravity effects as well as clinostat modeling effects on a cell may be attributed to the generalized unspecific reaction of a cell to external influence.     

Radiation protection guidance for activities in low-Earth orbit.

Scientific Committee 75 (SC 75) of the National Council on Radiation Protection and Measurements (NCRP) was assembled for the purpose of providing guidance to NASA concerning radiation protection in low-Earth orbit. The report of SC 75 was published in December 2000 as NCRP Report No. 132. In this presentation an overview of the findings and recommendations of the committee report will be presented.     

Comparison of genotoxic damage in monolayer cell cultures and three-dimensional tissue-like cell assemblies

Assessing the biological risks associated with exposure to the high-energy charged particles encountered in space is essential for the success of long-term space exploration. Although prokaryotic and eukaryotic cell models developed in our laboratory and others have advanced our understanding of many aspects of genotoxicity, in vitro models are needed to assess the risk to humans from space radiation insults. Such models must be representative of the cellular interactions present in tissues and capable of quantifying genotoxic damage. Toward this overall goal, the objectives of this study were to examine the effect of the localized microenvironment of cells, cultured as either 2-dimensional (2D) monolayers or 3-dimensional (3D) aggregates, on the rate and type of genotoxic damage resulting from exposure to Fe-charged particles, a significant portion of space radiation. We used rodent transgenic cell lines containing 50–70 copies of a LacI transgene to provide the enhanced sensitivity required to quantify mutational frequency and type in the 1100-bp LacI target as well as assessment of DNA damage to the entire 45-kbp construct. Cultured cells were exposed to high-energy Fe charged particles at Brookhaven National Laboratory’s Alternating Gradient Synchrotron facility for a total dose ranging from 0.1 to 2 Gy and allowed to recover for 0–7 days, after which mutational type and frequency were evaluated. The mutational frequency was found to be higher in 3D samples than in 2D samples at all radiation doses. Mutational frequency also was higher at 7 days after irradiation than immediately after exposure. DNA sequencing of the mutant targets revealed that deletional mutations contributed an increasingly high percentage (up to 27%) of all mutations in cells as the dose was increased from 0.5 to 2 Gy. Several mutants also showed large and complex deletions in multiple locations within the LacI target. However, no differences in mutational type were found between the 2D and the 3D samples. These 3D tissue-like model systems can reduce the uncertainty involved in extrapolating risk between in vitro cellular and in vivo models.     

Gravitropism of basidiomycetous fungi--on Earth and in microgravity.

In order to achieve perfect positioning of their lamellae for spore dispersal, fruiting bodies of higher fungi rely on the omnipresent force gravity. Only accurate negatively gravitropic orientation of the fruiting body cap will guarantee successful reproduction. A spaceflight experiment during the STS-55 Spacelab mission in 1993 confirmed that the factor gravity is employed for spatial orientation. Most likely every hypha in the transition zone between the stipe and the cap region is capable of sensing gravity. Sensing presumably involves slight sedimentation of nuclei which subsequently causes deformation of the net-like arrangement of F-actin filament strands. Hyphal elongation is probably driven by hormone-controlled activation and redistribution of vesicle traffic and vesicle incorporation into the vacuoles and cell walls to subsequently cause increased water uptake and turgor pressure. Stipe bending is achieved by way of differential growth of the flanks of the upper-most stipe region. After reorientation to a horizontal position, elongation of the upper flank hyphae decreases 40% while elongation of the lower flank slightly increases. On the cellular level gravity-stimulated vesicle accumulation was observed in hyphae of the lower flank.     

The dose rate observed on 19-21 October 1989 and its modulation by geophysical effects.

The Liulin dosimeter-radiometer on the MIR space station detected the 19 October 1989 high energy solar proton event. These results show that the main particle increase contains protons with energies up to about 9 GeV. After the main particle onset the Liulin dosimeter observed a typical geomagnetic cutoff modulation of the dose rate from the solar particles as the MIR space station traversed magnetic latitudes. When the interplanetary shock and associated solar plasma enveloped the earth on 20 October between 14 and 17 UT the radiation exposure increased significantly due to the lowering of the geomagnetic cutoff. The analysis of this event shows how various geophysical phenomena can significantly modulate the dose rate encountered by earth-orbiting spacecraft.     

Modifying factors on repair phenomena.

Every step of radiation damage repair can be modified by several factors like temporal and local energy distribution (LET), physiological conditions, biochemical and chemical composition of the target environment. Most interesting in the case of space radiation is the possible influence of high LET and of microgravity which could change the repair i.e. fusion capacity as well as the phenomenon of self assembly.