Food production and gas exchange system using blue-green alga (Spirulina) for CELSS.

In order to reduce the cultivation area required for the growth of higher plants in space adoption of algae, which have a higher photosynthetic ability, seems very suitable for obtaining oxygen and food as a useful source of high quality protein. The preliminary cultivation experiment for determining optimum cultivation conditions and for obtaining the critical design parameters of the cultivator itself has been conducted. Spirulina was cultivated in the 6-liter medium containing sodium hydrogen carbonate solution and a cultivation temperature controlled using a thermostat. Generated oxygen gas was separated using a polypropyrene porous hollow fiber membrane module. Through this experiment, oxygen gas (at a concentration of more than 46%) at a rate of 100-150 ml per minute could be obtained.     

Spectral composition of light and plant productivity.

Among other problems the Institute of Biophysics is working on the development of physiological and fundamental aspects of intensive light cultivation of higher plants. These technologies can be used in life support systems for stationary space station such as a Lunar base, a planetary base or a large orbital station. The source of energy may be the Sun or a nuclear reactor. In certain conditions, such sources of energy allow the use of a very broad range of irradiance of plants, in particular in the light energy range up to 2-3 times the solar energy (up to 100-1200 W/m2 PAR). Our Institute was the first to show that under such a high irradiance, some plants (radish, wheat, for example) can actively photosynthesize and exhibit high productivity on a sowing area basis. These results were later confirmed in the laboratory of Prof. Salisbury (USA).     

Performance of the CELSS Antarctic Analog Project (CAAP) crop production system.

Regenerative life support systems potentially offer a level of self-sufficiency and a decrease in logistics and associated costs in support of space exploration and habitation missions. Current state-of-the-art in plant-based, regenerative life support requires resources in excess of allocation proposed for candidate mission scenarios. Feasibility thresholds have been identified for candidate exploration missions. The goal of this paper is to review recent advances in performance achieved in the CELSS Antarctic Analog Project (CAAP) in light of the likely resource constraints. A prototype CAAP crop production chamber has been constructed and operated at the Ames Research Center. The chamber includes a number of unique hardware and software components focused on attempts to increase production efficiency, increase energy efficiency, and control the flow of energy and mass through the system. Both single crop, batch production and continuous cultivation of mixed crops production studies have been completed. The crop productivity as well as engineering performance of the chamber are described. For each scenario, energy required and partitioned for lighting, cooling, pumping, fans, etc. is quantified. Crop production and the resulting lighting efficiency and energy conversion efficiencies are presented. In the mixed-crop scenario, with 27 different crops under cultivation, 17 m2 of crop area provided a mean of 515 g edible biomass per day (85% of the approximate 620 g required for one person). Enhanced engineering and crop production performance achieved with the CAAP chamber, compared with current state-of-the-art, places plant-based life support systems at the threshold of feasibility.     

Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controlled [correction of controled] environment conditions.

To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 micromoles m-2 s-1 decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m2) of the wheat crops was attained at the irradiance of 920 micromoles m-2 s-1. Light intensity of 1150 micromoles m-2 s-1 decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m2) as compared to 920 micromoles m-2 s-1. The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 micromoles m-2 s-1 the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and micro-elements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems.     

Design for a bioreactor with sunlight supply and operations systems for use in the space environment.

An experiment was carried out to determine the characteristics of an operations system that can support fast cultivation of algae at high densities in the weightlessness of space. The experiment was conducted in glass bioreactor tanks, in which light was supplied by radiator rods connected to optical fiber cables. The illumination areas of the tanks were 2600 cm2, 6000 cm2, and 9200 cm2 per liter of solution. The characteristics of O2-CO2 gas exchange, concentration and separation of chlorella in the growth medium, dialysis of ionic salts in the growth medium, etc. were examined. Chloralla ellipsoidea was used in the experiment, yielding the following results: (1) By increasing the ratio of illumination area to volume, growth rates of up to approximately 0.6 g/L h could be obtained in a highly concentrated solution (one that contains 20 g/L or more of algae). (2) The most suitable proportions of carbon dioxide and oxygen gases for growing algae quickly at high concentrations were found to be 10% CO2 and 10% O2 (by volume). (3) There was a high optimum concentration for fast cultivation, and the data obtained resembled the theoretical curve postulated by P. Behrens et al. (4) It was possible to exchange carbon dioxide and oxygen using gas-permeable membrane modules. (5) It was possible to separate the chlorella from the growth medium and recycle the medium.     

Utilization of potatoes in bioregenerative life support systems.

Data on the tuberization, harvest index, and morphology of 2 cvs of white potato (Solanum tuberosum L.) grown at 12, 16, 20, 24 and 28 degrees C, 250, 400 and 550 micromoles s-1 m-2 photosynthetic photon flux (PPF), 350, 1000 and 1600 microliters l-1 CO2 will be presented. A productivity of 21.9 g m-2 day-1 of edible tubers from a solid stand of potatoes grown for 15 weeks with continuous irradiation at 400 micromoles s-1 m-2, 16 degrees C and 1000 microliters l-1 CO2 has been obtained. This equates to an area of 34.3 m2 being required to provide 2800 kcal of potatoes per day for a human diet. Separated plants receiving side lighting have produced 32.8 g m-2 day-1 which equates to an area of 23.6 m2 to provide 2800 kcal. Studies with side lighting indicate that productivities in this range should be realized from potatoes. Glycoalkaloid levels in tubers of controlled-environment-grown plants are within the range of levels found in tubers of field grown plants. The use and limitation of recirculating solution cultures for potato growth is discussed.     

气体介质对排气式气囊缓冲特性影响研究

中国新一代载人飞船试验船研制过程中开发了一套组合式缓冲气囊.以新飞船返回舱为研究对象,通过流体力学和热力学理论推导,建立了含有内囊的组合式气囊缓冲过程动力学模型,并通过MSC-Dytran对其有效性进行了验证.之后通过仿真计算,分析了影响气囊缓冲特性的两项气体固有参数——气体自由度与摩尔质量——对气囊缓冲过程的影响.研...     

Monitoring of long-term land subsidence from 2003 to 2017 in coastal area of Semarang,Indonesia by SBAS DInSAR analyses using Envisat-ASAR,ALOS-PALSAR,and Sentinel-1A SAR data

Land subsidence is a critical issue that large cities located in coastal areas, such as Semarang, Indonesia, must address. The monitoring of land subsidence is vital for predicting and mitigating the disasters that such subsidence may cause. Therefore, an economical and effective monitoring method, which can continuously provide accurate measurements over extensive areas, is highly required. Differential Interferometry Synthetic Aperture Radar (DInSAR) has the potential to be a powerful technique that can meet the above demands. Actually, DInSAR has been applied to monitor the subsidence in Semarang, but it was for a limited period before 2012.In order to clarify the transition of the long-term subsidence behavior in Semarang, the Small Baseline Subset (SBAS) method, which is one type of time-series DInSAR, is employed in this research. The sets of data of Envisat-ASAR (2003–2007), ALOS-PALSAR (2007–2011), and Sentinel-1A (2015–2017) are employed for the analyses. Then, the validity of the SBAS results is discussed from the viewpoints of both spatial distribution and temporal transition using GPS displacement measurement results and the geological conditions of the ground.On the other hand, as the lifespan of SAR satellites is commonly designed to be around 5–7?years, an appropriate method, which can connect the subsidence provided independently by the unlinked time-series data sets of the three different SAR satellite data, is required. This study uses the Hyperbolic Method (HM) to connect the above unlinked SBAS results. The HM is often used to fit the monitored subsidence in practice as a geotechnical engineering tool. Using this method, 14?years of the temporal behavior of the subsidence in Semarang is evaluated.It is found that the transition of the subsidence is different depending on the location, and that the subsidence rate is still increasing in the north and northeast parts of the coastal area. This study shows that SBAS DInSAR can be a useful tool for long-term continuous subsidence monitoring.     

Investigations on gel forming media for use in low gravity bioseparations research.

Microgravity research includes investigations designed to gain insight on methods of separating living cells. During a typical separation certain real-time measurements can be made by optical methods, but some materials must also be subjected to subsequent analyses, sometimes including cultivation of the separated cells. In the absence of on-orbit analytical or fraction collecting procedures, some means is required to "capture" cells after separation. The use of solutions that form gels was therefore investigated as a means of maintaining cells and/or macromolecules in the separated state after two types of simple ground-based experiments. Microgravity electrophoresis experiments were simulated by separating model cell types (rat, chicken, human and rabbit erythrocytes) in a vertical density gradient containing low-conductivity buffer, 1.7%-6.5% Ficoll, 6.8-5.0% sucrose, and 1% SeaPrep low-melting temperature agarose and demonstrating that, upon cooling, a gel formed in the column, and cells could be captured in the positions to which they had migrated. Two-phase extraction experiments were simulated by choosing two-polymer solutions in which phase separation occurs in normal saline at temperatures compatible with cell viability and in which one or both phases form a gel upon cooling. Suitable polymers included commercial agaroses (1-2%), maltodextrin (5-7%) and gelatin (5-20%).     

Plant growth and gas balance in a plant and mushroom cultivation system.

In order to obtain basic data for construction of a plant cultivation system incorporating a mushroom cultivation subsystem in the CELSS, plant growth and atmospheric CO2 balance in the system were investigated. The plant growth was promoted by a high level of CO2 which resulted from the respiration of the mushroom mycelium in the system. The atmospheric CO2 concentration inside the system changed significantly due to the slight change in the net photosynthetic rate of plants and/or the respiration rate of the mushroom when the plant cultivation system combined directly with the mushroom cultivation subsystem.     

武装直升机在杀爆弹打击下的易损性及防护策略

为研究武装直升机对小口径杀爆（HE）弹的防护策略，建立了某型武装直升机在小口径杀爆弹打击下的易损性模型，计算了典型受攻击方向的武装直升机易损面积，并将武装直升机底面划分为不同区域，计算了各区域的防护效率。计算表明，武装直升机最易损方向为其右后下方，此方向的易损面积为6.97 m2，结合武装直升机的外形特征，得出底面和侧面应当优先防护。对于武装直升机底面，前后燃油箱所在的2个区域的防护效率均达到50%以上，在布置装甲重量有限的条件下可优先防护该区域。另外，在主机身与机尾舱之间增加隔板可阻挡弹丸爆炸后横向飞散的破片，与直接防护武装直升机尾部相比，其防护效率由2.93%提高至11.07%。      

Development of in-situ Space Debris Detector

Due to high relative velocities, collisions of spacecraft in orbit with Space Debris (SD) or Micrometeoroids (MM) can lead to payload degradation, anomalies as well as failures in spacecraft operation, or even loss of mission. Flux models and impact risk assessment tools, such as MASTER (Meteoroid and Space Debris Terrestrial Environment Reference) or ORDEM (Orbital Debris Engineering Model), and ESABASE2 or BUMPER II are used to analyse mission risk associated with these hazards. Validation of flux models is based on measured data. Currently, as most of the SD and MM objects are too small (millimeter down to micron sized) for ground-based observations (e.g. radar, optical), the only available data for model validation is based upon retrieved hardware investigations e.g. Long Duration Exposure Facility (LDEF), Hubble Space Telescope (HST), European Retrievable Carrier (EURECA). Since existing data sets are insufficient, further in-situ experimental investigation of the SD and MM populations are required. This paper provides an overview and assessment of existing and planned SD and MM impact detectors. The detection area of the described detectors is too small to adequately provide the missing data sets. Therefore an innovative detection concept is proposed that utilises existing spacecraft components for detection purposes. In general, solar panels of a spacecraft provide a large area that can be utilised for in-situ impact detection. By using this method on several spacecraft in different orbits the detection area can be increased significantly and allow the detection of SD and MM objects with diameters as low as 100 μm. The design of the detector is based on damage equations from HST and EURECA solar panels. An extensive investigation of those panels was performed by ESA and is summarized within this paper. Furthermore, an estimate of the expected sensitivity of the patented detector concept as well as examples for its implementation into large and small spacecraft are presented.     

NASA's Biomass Production Chamber: a testbed for bioregenerative life support studies.

The Biomass Production Chamber (BPC) located at Kennedy Space Center, FL, USA provides a large (20 m2 area, 113 m3 vol.), closed environment for crop growth tests for NASA's Controlled Ecological Life Support System (CELSS) program. Since the summer of 1988, the chamber has operated on a near-continuous basis (over 1200 days) without any major failures (excluding temporary power losses). During this time, five crops of wheat (64-86 days each), three crops of soybean (90 to 97 days), five crops of lettuce (28-30 days), and four crops of potato (90 to 105 days were grown, producing 481 kg of dry plant biomass, 196 kg edible biomass, 540 kg of oxygen, 94,700 kg of condensed water, and fixing 739 kg of carbon dioxide. Results indicate that total biomass yields were close to expected values for the given light input, but edible biomass yields and harvest indices were slightly lower than expected. Stand photosynthesis, respiration, transpiration, and nutrient uptake rates were monitored throughout growth and development of the different crops, along with the build-up of ethylene and other volatile organic compounds in the atmosphere. Data were also gathered on system hardware maintenance and repair, as well as person-hours required for chamber operation. Future tests will include long-term crop production studies, tests in which nutrients from waste treatment systems will be used to grow new crops, and multi-species tests.     

Food production and nutrition in Biosphere 2: results from the first mission September 1991 to September 1993.

The initial test of the Biosphere 2 agricultural system was to provide a nutritionally adequate diet for eight crew members during a two year closure experiment, 1991-1993. The overall results of that trial are presented in this paper. The 2000 m2 cropping area provided about 80 percent of overall nutritional needs during the two years. Adaptation of the crew to the diet which averaged 2200 calories, 73 g. of protein and 32 g. of fat per person over the course of the two years. The diet was primarily vegetarian, with only small amounts of milk, meat and eggs from the system's domestic animals. The crew experienced 10-20 percent weight loss, most of which occurred in the first six months of the closure reflecting adaptation to the diet and lower caloric intake during that period. Since Biosphere 2 is a tightly sealed system, non-toxic methods of pest and disease control were employed and inedible plant material, domestic animal wastes and human waste-water were processed and nutrients returned to the soil. Crop pests and diseases, especially broad mites and rootknot nematode, reduced yields, and forced the use of alternative crops. Outstanding crops included rice, sweet potato, beets, banana, and papaya. The African pygmy goats were the most productive of the domestic animals. Overall, the agriculture and food processing required some 45% of the crew time.     

吸气式电推进系统可行条件分析

吸气式电推进系统作为有可能实现长寿命超低轨飞行的技术而被关注。根据不同轨道环境条件，采用管状结构进气道、以及机械增压的吸气方式，讨论了吸气式电推进系统所需的可行条件。分析表明，在轨高度180~240km，航天器所需总功耗与迎风面之比需要大于2kW/m2，电推力器比冲需大于4×104m/s，方可满足推阻平衡需求。分析得出，实现吸气式系统在地球轨道的运用，关键技术在于增加气体收集效率并且降低收集功耗，同时电推力器的效率还需进一步提升。     

Autonomous satellite navigation using starlight refraction angle measurements

An on-board autonomous navigation capability is required to reduce the operation costs and enhance the navigation performance of future satellites. Autonomous navigation by stellar refraction is a type of autonomous celestial navigation method that uses high-accuracy star sensors instead of Earth sensors to provide information regarding Earth’s horizon. In previous studies, the refraction apparent height has typically been used for such navigation. However, the apparent height cannot be measured directly by a star sensor and can only be calculated by the refraction angle and an atmospheric refraction model. Therefore, additional errors are introduced by the uncertainty and nonlinearity of atmospheric refraction models, which result in reduced navigation accuracy and reliability. A new navigation method based on the direct measurement of the refraction angle is proposed to solve this problem. Techniques for the determination of the refraction angle are introduced, and a measurement model for the refraction angle is established. The method is tested and validated by simulations. When the starlight refraction height ranges from 20 to 50 km, a positioning accuracy of better than 100 m can be achieved for a low-Earth-orbit (LEO) satellite using the refraction angle, while the positioning accuracy of the traditional method using the apparent height is worse than 500 m under the same conditions. Furthermore, an analysis of the factors that affect navigation accuracy, including the measurement accuracy of the refraction angle, the number of visible refracted stars per orbit and the installation azimuth of star sensor, is presented. This method is highly recommended for small satellites in particular, as no additional hardware besides two star sensors is required.     

n:k(G)/m系统可靠性

带有m个交叉冷贮备部件的“n中取k好”表决系统结构可用于通信/广播卫星的转发器系统。称这种系统结构为n∶k(G)/m系统。本文给出这种系统可靠性的计算公式。文中还给出3∶2(G)/1转发器系统的转换开关设计,它可以实现交叉切换功能。     

Life sciences flight hardware development for the International Space Station.

During the construction phase of the International Space Station (ISS), early flight opportunities have been identified (including designated Utilization Flights, UF) on which early science experiments may be performed. The focus of NASA's and other agencies' biological studies on the early flight opportunities is cell and molecular biology; with UF-1 scheduled to fly in fall 2001, followed by flights 8A and UF-3. Specific hardware is being developed to verify design concepts, e.g., the Avian Development Facility for incubation of small eggs and the Biomass Production System for plant cultivation. Other hardware concepts will utilize those early research opportunities onboard the ISS, e.g., an Incubator for sample cultivation, the European Modular Cultivation System for research with small plant systems, an Insect Habitat for support of insect species. Following the first Utilization Flights, additional equipment will be transported to the ISS to expand research opportunities and capabilities, e.g., a Cell Culture Unit, the Advanced Animal Habitat for rodents, an Aquatic Facility to support small fish and aquatic specimens, a Plant Research Unit for plant cultivation, and a specialized Egg Incubator for developmental biology studies. Host systems (Figure 1A, B: see text), e.g., a 2.5 m Centrifuge Rotor (g-levels from 0.01-g to 2-g) for direct comparisons between g and selectable g levels, the Life Sciences Glovebox for contained manipulations, and Habitat Holding Racks (Figure 1B: see text) will provide electrical power, communication links, and cooling to the habitats. Habitats will provide food, water, light, air and waste management as well as humidity and temperature control for a variety of research organisms. Operators on Earth and the crew on the ISS will be able to send commands to the laboratory equipment to monitor and control the environmental and experimental parameters inside specific habitats. Common laboratory equipment such as microscopes, cryo freezers, radiation dosimeters, and mass measurement devices are also currently in design stages by NASA and the ISS international partners.     

南极中山站宇宙线观测

2014年由两个具有0.5m×0.5m闪烁体面积的探测器组成的宇宙线μ子望远镜在南极中山站建成,观测数据通过卫星链路传回并发布.通过对中山站μ子望远镜数据的气象效应分析,发现中山站的气压变化对宇宙线计数率影响显著并呈负相关.计算得到宇宙线高能质子在中山站的垂直截止刚度R_C为0.076GV,对比中国西藏羊八井和北京高能质子的垂直截止刚度,中山站非常有利于对太阳高能质子事件的观测.      

An experimental system for determining the influence of microgravity on B lymphocyte activation and cell fusion.

The influence of microgravity on lymphocyte activation is central to the understanding of immunological function in space. Moreover, the adaptation of groundbased technologies to microgravity conditions presents opportunities for biotechnological applications including high efficiency production of antibody forming hybridomas. Because the emerging technology of microgravity hybridoma generation is dependent upon activation and cultivation of B lymphocytes during flight, we have adapted mitogen-driven B lymphocyte stimulation and culture that allows for the in vitro generation of large numbers of antibody forming cells suitable for cell fusion over a period of 1-2 weeks. We believe that this activation and cultivation system can be flown on near-term space flights to test fundamental hypotheses about mammalian cell activation, cell fusion, metabolism, secretion, growth, and bio-separation.