On the influence of altered gravity on the growth of fish inner ear otoliths

Inner ear stones (otoliths) of developing cichlid fish (Oreochromis mossambicus) were marked with the calcium tracer alizarin-complexone (AC) at 1g-earth gravity before and after a long-term (20 days) stay of the animals at moderate hypergravity conditions (3g; centrifuge). AC deposition at the otoliths resulted in two fluorescence bands, which enclosed the area grown during exposure to altered gravity. This area was measured with regard to size and asymmetry (size difference between the left and the right stones).

Both utricular and saccular otoliths (lapilli and sagittae, respectively) were significantly smaller after hyper-g exposure as compared to parallely raised 1g-control specimens. The asymmetry concerning the lapilli was pronouncedly decreased in comparison to the 1g-controls. These findings suggest, that the growth and the development of bilateral asymmetry of otoliths is guided by the environmental gravity vector.

Some of the hyper-g animals revealed a kinetotic behaviour at the transfer from hyper-g to normal 1g-earth gravity conditions, which was qualitatively similar to the behaviour observed in previous experiments at the transfer from 1g to microgravity in the course of parabolic aircraft flights. The lapillar asymmetry of kinetotic samples was found to be significantly higher than that of normally behaving experimental specimens. This result supports an earlier theoretical concept, according to which human static space sickness might be based on asymmetric utricular otoliths.     

Artificial gravity--head movements during short-radius centrifugation: influence of cognitive effects

Short-radius centrifugation is a potential countermeasure against the effects of prolonged weightlessness. Head movements in a rotating environment, however, induce serious side effects: inappropriate vestibular ocular reflexes (VOR), body-tilt illusions and motion sickness induced by cross-coupled accelerations on a rotating platform. These are well predicted by a semicircular canal model. The present study investigates cognitive effects on the inappropriate VOR and the illusory sensations experienced by subjects rotating on a short-radius centrifuge (SRC). Subjects (N=19) were placed supine on a rotating horizontal bed with their head at the center of rotation. To investigate the extent to which they could control their sensations voluntarily, subjects were asked alternatively to "fight" (i.e. to try to resist and suppress) those sensations, or to "go" with (i.e. try to enhance or, at least, acquiesce in) them. The only significant effect on the VOR of this cognitive intervention was to diminish the time constant characterizing the decay of the nystagmus in subjects who had performed the "go" (rather than the "fight") trials. However, illusory sensations, as measured by reported subjective intensities, were significantly less intense during the "fight" than during the "go" trials. These measurements also verified an asymmetry in illusory sensation known from earlier experiments: the illusory sensations are greater when the head is rotated from right ear down (RED) to nose up (NU) posture than from NU to RED. The subjects habituated, modestly, to the rotation between their first and second sequences of trials, but showed no better (or worse) suppression of illusory sensations thereafter. No significant difference in habituation was observed between the "fight" and "go" trials.     

Main medical results of extended flights on space station Mir in 1986-1990

During 1986-1990 seven prime spacecrews (16 cosmonauts) have flown on-board the Mir orbital complex. The longest space mission duration was 366 days The principal objectives of the medical tasks were the maintenance of good health and performance of the spacecrews and conducting medical research programs which included study of the cardiovascular, motor, endocrine, blood, immune, and metabolic systems. Results obtained point to the ability of humans to readily adapt to a year-long stay in space and maintain good health and performance. Readaptation had a similar course as after other previous long-term space flights of up to 8 months in duration. Primary body system changes were not qualitatively different from findings after flights aboard the Salyut 6 and 7 space stations. In this case, during and after an 11-12 month flight, body system alterations were even less severe which was a result of adequate countermeasure use, their systematic and creative employment and maintenance of required environments to support life and work in space.     

Biological induced corrosion of materials II: New test methods and experiences from mir station

During previous long-term manned missions, more than 100 species of microorganisms have been identified on surfaces of materials (bacteria and fungi). Among them were potentially pathogenic ones (saprophytes) which are capable of active growth on artificial substrates, as well as technophilic bacteria and fungi causing damages (destruction and degradation) to various materials (metals and polymers), resulting in failures and disruptions in the functioning of equipment and hardware.

Aboard a space vehicle some microclimatic parameters are optimal for microorganism growth: the atmospheric fluid condensate with its specific composition, chemical and/or antropogenic contaminants (human metobolic products, etc.) all are stimulating factors for the development of bacteria and mould fungi on materials of the interior and equipment of an orbital station during its operational phase(s).

Especially Russian long-term missions (SALJUT, MIR) have demonstrated that uncontrolled interactions of microorganisms with materials will ultimately lead to the appearence of technological and medical risks, significantly influencing safety and reliability characteristics of individual as well as whole systems and/ or subsystems.

For a first conclusion, it could be summarized, that countermeasures and anti-strategies focussing on Microbial Contamination Management (MCM) for the International Space Station (ISS, next long-term manned mission) at least require a new materials test approach.

Our respective concept includes a combined age-ing/biocorrosion test sequence. It is represented here, as well as current status of MCM program, e.g. continuous monitoring (microbiological analyses), long-term disinfection, frequent cleaning methods, mathematical modeling of ISS, etc.     

Adaptational changes in the neural control of cardiorespiratory function in a confined environment: the CNEC#3 experiment

The goal of the study was to characterize the changes in neurovegetative control of the circulation, attending the presumed physiological and psychological stress originated by the isolation and confinement typical of the living condition of space stations, as simulated in a ground based unit, using time and frequency domain analysis. As a secondary goal we sought to verify the implementation of real time data acquisition, for off line spectral analisys of R-R interval, systolic arterial pressure (by Finapres) and respiration (by PVF2 piezoelectric sensors).

We addressed the cardiorespiratory and neurovegetative responses to standardized, simple Stressors (active standing, dynamic and static handgrip) on the EXEMSI 92 crew, before, during and after the isolation period.

On average the appropriate excitatory responses (to stand, dynamic and static handgrip) were elicited also in isolation and confinement.

Active standing and small masses muscular exercises are easy to be performed in a confined and isolated environment and provide a valuable tool for investigating the adaptational changes in neural control mechanisms.

The possibility there exists of using this time and frequency domain approach to monitor the level of performance and well being of the space crew in (quasi) real time.     

Fluid and salt supplementation effect on body hydration and electrolyte homeostasis during bed rest and ambulation

Bed rest (BR) induces significant urinary and blood electrolyte changes, but little is known about the effect of fluid and salt supplements (FSS) on catabolism, hydration and electrolytes. The aim was to measure the effect of FSS on catabolism, body hydration and electrolytes during BR.

Studies were done during 7 days of a pre-bed rest period and during 30 days of a rigorous bed rest period. Thirty male athletes aged, 24.6±7.6 years were chosen as subjects. They were divided into three groups: unsupplemented ambulatory control subjects (UACS), unsupplemented bed rested subjects (UBRS) and supplemented bed rested subjects (SBRS). The UBRS and SBRS groups were kept under a rigorous bed rest regime for 30 days. The SBRS daily took 30 ml water per kg body weight and 0.1 sodium chloride per kg body weight.

Plasma sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg) levels, urinary Na, K, Ca and Mg excretion, plasma osmolality, plasma protein level, whole blood hemoglobin (Hb) and hematocrit (Hct) level increased significantly (p≤0.05), while plasma volume (PV), body weight, body fat, peak oxygen uptake, food and fluid intake decreased significantly (p≤0.05) in the UBRS group when compared with the SBRS and UACS groups. In contrast, plasma and urinary electrolytes, osmolality, protein level, whole blood Hct and Hb level decreased significantly (p≤0.05), while PV, fluid intake, body weight and peak oxygen uptake increased significantly (p≤0.05) in the SBRS group when compared with the UBRS group. The measured parameters did not change significantly in the UACS group when compared with their baseline control values.

The data indicate that FSS stabilizes electrolytes and body hydration during BR, while BR alone induces significant changes in electrolytes and body hydration. We conclude that FSS may be used to prevent catabolism and normalize body hydration status and electrolyte values during BR.     

Excretion of amino acids by humans during space flight

We measured the urine amino acid distribution patterns before, during and after space flight on the Space Shuttle. The urine samples were collected on two separate flights of the space shuttle. The first flight lasted 9.5 days and the second flight 15 days. Urine was collected continuously on 8 subjects for the period beginning 10 d before launch to 6 d after landing. Results: In contrast to the earlier Skylab missions where a pronounced amino aciduria was found, on shuttle the urinary amino acids showed little change with spaceflight except for a marked decrease in all of the amino acids on FD (flight day) 1 (p<0.05) and a reduction in isoleucine and valine on FD3 and FD4 (p<0.05). Conclusions: (i) Amino aciduria is not an inevitable consequence of space flight. (ii) The occurrence of amino aciduria, like muscle protein breakdown is a mission specific effect rather than part of the general human response to microgravity.     

Rosetta: The ESA comet rendezvous mission

Rosetta was selected in November 1993 for the ESA Cornerstone 3 mission, to be launched in 2003, dedicated to the exploration of the small bodies of the solar system (asteroids and comets). Following this selection, the Rosetta mission and its spacecraft have been completely reviewed: this paper presents the studies performed the proposed mission and the resulting spacecraft design.

Three mission opportunities have been identified in 2003–2004, allowing rendezvous with a comet. From a single Ariane 5 launch, the transfer to the comet orbit will be supported by planetary gravity assists (two from Earth, one from Venus or Mars); during the transfer sequence, two asteroid fly-bys will occur, allowing first mission science phases. The comet rendezvous will occur 8–9 years after launch; Rosetta will orbit around the comet and the main science mission phase will take place up to the comet perihelion (1–2 years duration).

The spacecraft design is driven (i) by the communication scenario with the Earth and its equipment, (ii) by the autonomy requirements for the long cruise phases which are not supported by the ground stations, (iii) by the solar cells solar array for the electrical power supply and (iv) by the navigation scenario and sensors for cruise, target approach and rendezvous phases. These requirements will be developed and the satellite design will be presented.     

Research and development of On-Board Processor for advanced mobile satellite communications

This paper describes the research and development of an On-Board Processor (OBP) for a mobile satellite communications system using a geostationaly satellite. The system, which based on Multi-Carrier Time Division Multiple Access, can realize high performance and facilitate the use of miniature hand-held mobile Earth terminals.

Our research and development results indicate as follows.

1. 1) The simultaneous-slot switching method will be the most suitable for an on-board base-band switching with consideration of traffic capacity, time delay, and hardware scale.

2. 2) The OBP performs channel exchange with regeneration that contributes to a reduction in the size of mobile terminals because of the coding gain.

3. 3) Many kinds of Application Specific Integrated Circuits are designed to realize large channel capacity and reduce the size and power consumption of the OBP. These OBP design can increase the channel capacity, which is equivalent to in 5.6kbps voice channel, up to over 500 channels within 5MHz frequency band

Some results are applied to the OBP being developed for the Japanese Engineering Test Satellite VII (ETS-VB).     

Dosimetric measurements in manned missions

Detector packages consisting of thermoluminescence detectors (TLDs), nuclear emulsions and plastic nuclear track detectors were exposed in different sections of the MIR space station, inside the Spacelab during the IML1 mission, and inside Spacelab module and tunnel during the D2 mission. This report concentrates on total dose measurements with TLDs during these mission. The results are discussed and compared to results of former missions and to calculations. Finally, dose equivalents and mean quality factors for each mission are presented which are derived from the TLD results and results obtained from the other detector systems. Dose equivalents range between 200 μSvd⁻¹ and 700 μSvd⁻¹.     

Future earth observation missions for oceanographic applications: Indian perspectives

Significant progress has been achieved in India in demonstrating the utility of remote sensing data for various oceanographic applications during the last one decade. Among these, techniques have been developed for retrieval of ocean surface waves, winds, wave forecast model, internal waves, sea surface temperature and chlorophyll pigments. Encouraged from these results as well as for meeting the specific and increasing data requirements on an assured basis by oceanographers, India is making concerted efforts for developing and launching state-of-the-art indigenous satellites for ocean applications in the coming years.

The first in the series of ocean satellites planned for launch is Oceansat-1 (IRS-P4) by early 1999. Oceansat-1 carries on-board an Ocean Colour Monitor (OCM) and a Multi-frequency Scanning Microwave Radiometer (MSMR). OCM will have 8 narrow spectral bands operating in visible and near- infrared bands (402–885 nm) with a spatial resolution of 360 m and swath of 1420 km. The MSMR with its all weather capability is configured to have measurements at 4 frequencies viz., 6.6, 10.65, 18 & 21 GHz in dual polarisation mode with a spatial resolution of 120, 80, 40 & 40 km, respectively with an overall swath of 1360 km. The Oceansat-1 with repetitivity of once in two days will provide global data for retrieval of various oceanographic and meteorological parameters such as chlorophyll (primary productivity), sea surface temperature and wind speed, besides a host of other parameters of relevance to meteorology.

A full fledged satellite for ocean applications known as Oceansat-2 (IRS-P7) is also planned for launch during 2002. This satellite with payload mix of microwave (Scatterometer, Altimeter & Passive Microwave Radiometer), Thermal (TIR) and Optical (OCM) sensors, will provide greater in-sight into the global understanding of ocean dynamics/resources. This mission is expected to provide a complete set of oceanographic measurements, which are useful for providing operational oceanographic services.

Efforts are also on towards development of missions having multi-frequency, multipolarisation and multi-look angle microwave payloads including Synthetic Aperture Radar (SAR) and advanced millimeter wave sounders, besides development of imaging spectrometers by 2005.

A well-knit plan has been initiated in India for utilisation of planned Oceansat data. Important efforts initiated in this direction include SATellite Coastal and Oceanographic Research and Ocean Information Services, which are being carried out on an integrated basis aiming at providing services to the down stream users. The paper highlights these efforts in India towards providing an operational ocean information services in the coming years.     

The response of structure and function of the gravireceptor in a vertebrate to near weightlessness.

The paper sums up results of a 7-day space flight experiment (D-l-Mission-BW-STA 00-STATEX) using growing frog embryos and larvae (Xenopus laevis) as a model system. Evaluation of photographs taken from the surface of sectioned deep-frozen objects, and micrographs using TEM and SEM show no aberrations in the shape, size, position, or respective electron density of the otolith membranes in larvae developed for 154 h under near-zero g. The further evaluation of the "weightless larvae" revealed a probably not yet described otolith-like formation below the dorsal wall of the vestibulum. In the weightless larvae this formation outnumbers, also qualitatively, strongly the 1-g control samples. The swimming behavior of the tadpoles which was observed about one hour after landing of the Space Shuttle showed a typical anomaly (loop swimming), which is known from larvae developed on the clinostat or from fish flown aboard Apollo capsules. An extra result is the lack of striking effects of cosmic radiation on the embryonic development of the flown Xenopus eggs.     

Cardiac and vascular adaptation to 0g with and without thigh cuffs (Antares 14 and Altair 21 day Mir spaceflights)

Cardiovascular Actaptation was evaluated on 2 astronauts: one wearing thigh cuffs from flight day 1 to 8 (14d flight), the second without cuffs (21d flight). Ultrasound investigations were performed at rest and during LBNP. Results: Without thigh cuffs the cardiovascular Actaptation consists in (1) the development of a hypovolemia with an increase of the heart rate and the cardiac output, (2) the decrease of the vascular tone in the deep (mesenteric and splanchnic) and peripheral (Lower limbs) vascular areas. The use of thigh cuffs maintains the volemia and the cardiac output at the preflight level (without heart rate increase) and prevents the loss of vascular tone in the deep and peripheral areas. Moreover the adaptative process changes since the cuffs are removed and even the volemia seems to be unaffected at this stage the vascular tone decreases to a comparable extend as during the flight without cuffs. Nevertheless during the flight without cuffs or 3 days after removing the cuffs hemodynamic signs of decreased orthostatic tolerance are present during the inflight and the 3 days post flight LBNP. Presently the possible contribution of the thigh cuffs to the reduction of the vascular deconditioning has not been tested yet.     

Planetary Defense From Space: Part 1-Keplerian Theory

A system of two space bases housing missiles is proposed to achieve the Planetary Defense of the Earth against dangerous asteroids and comets. We show that the layout of the Earth–Moon system with the five relevant Lagrangian (or libration) points in space leads naturally to only one, unmistakable location of these two space bases within the sphere of influence of the Earth. These locations are at the two Lagrangian points L1 (in between the Earth and the Moon) and L3 (in the direction opposite to the Moon from the Earth).

We show that placing bases of missiles at L1 and L3 would cause those missiles to deflect the trajectory of asteroids by hitting them orthogonally to their impact trajectory toward the Earth, so as to maximize their deflection. We show that the confocal conics are the best class of trajectories fulfilling this orthogonal deflection requirement.

An additional remark is that the theory developed in this paper is just a beginning of a larger set of future research work. In fact, while in this paper we only develop the Keplerian analytical theory of the Optimal Planetary Defense achievable from the Earth–Moon Lagrangian points L1 and L3, much more sophisticated analytical refinements would be needed to:

1. Take into account many perturbation forces of all kinds acting on both the asteroids and missiles shot from L1 and L3;

2. add more (non-optimal) trajectories of missiles shot from either the Lagrangian points L4 and L5 of the Earth–Moon system or from the surface of the Moon itself;

3. encompass the full range of missiles currently available to the US (and possibly other countries) so as to really see “which asteroids could be diverted by which missiles”, even in the very simplified scheme outlined here.

Outlined for the first time in February 2002, our Confocal Planetary Defense concept is a Keplerian Theory that proved simple enough to catch the attention of scholars, representatives of the US Military and popular writers. These developments could possibly mark the beginning of an “all embracing” mathematical vision of Planetary Defense beyond all learned activities, dramatic movies and unknown military plans covered by secret.     

Pathophysiology of motor functions in prolonged manned space flights.

The influence of weightlessness on different parts of the motor system have been studied in crew members of 140 and 175 days space flights. It has been shown that weightlessness affects all parts of the motor system including (i) the leg and trunk muscles, in which severe atonia, a decrease of strength and an increase of electromyographic cost of contraction have been observed, (ii) the proprioceptive elements and the spinal reflex mechanisms in which decreased thresholds accompanied by decreases of maximal amplitude of reflexes and disturbances in cross reflex mechanisms have been found. and (iii) the central mechanisms that control characteristics of postural and locomotor activities. The intensities and durations of disturbances of different parts of the motor system did not correlate to each other, but did correlate with prophylactic activity during space flight. The data suggest a different nature of disturbances caused by weightlessness in different parts of the motor system.     

Assessment of the efficacy of medical countermeasures in space flight

Changes in body fluids, electrolytes, and muscle mass are manifestations of adaptation to space flight and readaptation to the 1-g environment. The purposes of this paper are to review the current knowledge of biomedical responses to short- and long-duration space missions and to assess the efficacy of countermeasures to 1-g conditioning. Exercise protocols, fluid hydration, dietary and potential pharmacologic measures are evaluated, and directions for future research activities are recommended.     

去型号化接收机地面环境模拟试验充分性验证

为考察针对去型号化接收机的地面各项环境模拟试验是否能充分有效验证在轨工作环境，利用高灵敏度接收机对噪声的敏感特性，选取通道AGC作为环境模拟试验充分性验证参数；根据多台去型号化接收机的地面试验数据，分析多批次接收机的AGC参数一致性，得到去型号化接收机通道参数差异很小的结论；在此基础上，通过对多台高灵敏度接收机在轨AGC数据与地面环境试验AGC数据的比对，明确了由环境差异导致的AGC数值差异范围，可以为后续产品的地面环境试验和在轨飞行提供参考。对多台接收机在轨飞行接收机通道底噪变小的现象进行分析，得到地面环境试验模拟的电磁环境条件比空间电磁环境更恶劣的结论。     

An energy balance concept for habitability

Habitability can be formulated as a balance between the biological demand for energy and the corresponding potential for meeting that demand by transduction of energy from the environment into biological process. The biological demand for energy is manifest in two requirements, analogous to the voltage and power requirements of an electrical device, which must both be met if life is to be supported. These requirements exhibit discrete (non-zero) minima whose magnitude is set by the biochemistry in question, and they are increased in quantifiable fashion by (i) deviations from biochemically optimal physical and chemical conditions and (ii) energy-expending solutions to problems of resource limitation. The possible rate of energy transduction is constrained by (i) the availability of usable free energy sources in the environment, (ii) limitations on transport of those sources into the cell, (iii) upper limits on the rate at which energy can be stored, transported, and subsequently liberated by biochemical mechanisms (e.g., enzyme saturation effects), and (iv) upper limits imposed by an inability to use "power" and "voltage" at levels that cause material breakdown. A system is habitable when the realized rate of energy transduction equals or exceeds the biological demand for energy. For systems in which water availability is considered a key aspect of habitability (e.g., Mars), the energy balance construct imposes additional, quantitative constraints that may help to prioritize targets in search-for-life missions. Because the biological need for energy is universal, the energy balance construct also helps to constrain habitability in systems (e.g., those envisioned to use solvents other than water) for which little constraint currently exists.