Analog environments for a Europa lander mission

This paper reviews the utility of analog environments in preparations for a Europa lander mission. Such analogs are useful in the demonstration and rehearsal of engineering functions such as sample acquisition from an icy surface, as well as in the exercise of the scientific protocols needed to identify organic, inorganic and possible biological impurities in ice. Particular attention is drawn to Antarctic and Arctic analog sites where progress in these latter areas has been significant in recent years.     

一种鲁棒的图像小波变换模拟编码传输方法

在复杂度和延迟受限或者可变信道条件下,由于通信系统的一体化设计,信源信道联合编码(JSCC, Joint Source-Channel Coding)技术的应用有助于改善传统的分离结构系统.针对带宽功率限制模拟信道,提出了一种结合了小波变换编码和香农映射信源信道联合编码的静止图像模拟编码传输方法;并基于更加合理的考虑了信道噪声的重建图像失真估计,提出了相应的联合优化带宽分配算法.仿真及分析表明,由于信源信道的联合优化及具有模拟编码传输的特点,该方法表现出良好的编码传输性能,且编解码复杂度和延迟小;同时在信道失配情况下表现出的柔性降级/改善特性有助于提高可变信道条件下系统的鲁棒性和信道带宽利用率.      

小型化星载模拟预失真器的研制

基于肖特基二极管(Schottlky Barrier Diode, SBD)的非线性提出一种新型的小型化、低成本的星载模拟线性化器,可以用来改善卫星测控系统中固态功放在饱和功率输出时引起的非线性失真。首先分析了模拟预失真器的工作原理,给出其等效电路模型,然后采用软件设计和仿真了一个新型的小型化星用模拟预失真器,将设计的电路加工制作并测试。结果表明该线性化器与功放级联后,能够有效改善功放在饱和功率输出时的3阶交调约6dB。     

Structure and NIR feature of QCC: a laboratory analog of carbon dust.

We have produced thin films of quenched carbonaceous composite (QCC) by hydrocarbon plasma deposition. The effect of thermal annealing on QCC has been investigated to understand how QCC, as a laboratory analog of carbon dust, is transformed in the warm environment around evolved stars. Spectroscopic measurements have indicated that, by heating, the proportion of aromatic sp2 CH bonds increases relative to sp3 CH bonds. Carbon onion-like spherules of approximately 10 nm in diameter are found with electron microscopic images after "graphitization" of thermal annealing.     

Radiation biology in space: a critical review.

A short summary of the results of radiobiological studies in space or on respective particles on ground will be given. Among the various types of radiation in space, the effect of heavy ions with high energy (HZE-particles) are most essential. Thus, radiobiology in space concerns mostly to the effect of these particles, in cells and in whole organism. Cell death, mutation and malignant transformation are the relevant endpoints, with can be studied on ground with heavy ions of different energy with suitable accelerators or in space, especially by the BIOSTACK concept. In space, however, the effect of microgravity has to be considered as well and there are hints, that under weightlessness the biological effect of radiation may be enhanced. There are still open questions to be answered concerning radioprotection of man in space. Further experiments are necessary.     

Quenched Carbonaceous Composite: a laboratory analog for carbonaceous material in the interstellar medium.

We review the properties of Quenched Carbonaceous Composite (QCC), a residue produced from a hydrocarbon plasma, and the properties of its derivatives. A. Sakata and his colleagues have shown that QCC has a 220 nm absorption band, visible fluorescence matching the extended red emission seen in reflection nebulae, and infrared absorption bands that correspond to the infrared emission features in reflection nebulae, HII regions, and planetary nebulae. These properties make QCC a strong candidate material as a laboratory analog to the carbonaceous material in the interstellar medium. QCC is distinguished from the PAH hypothesis in that (1) it is a condensate composed of aromatic and aliphatic molecules, as well as radicals; (2) it exhibits a 220 nm absorption that is very similar in wavelength to the 217 nm absorption in the interstellar medium; (3) it exhibits visible fluorescence consistent with that seen in reflection nebulae; and (4) the bands at 7.7 and 8.6 microns are caused by ketone bands in oxidized QCC. The aromatic component in QCC is thought to be typically 1-4 rings, with the majority being about 1-2 rings.     

Influence of microgravity on mitogen binding and cytoskeleton in Jurkat cells.

The effects of microgravity on Jurkat cells--a T-lymphoid cell line--was studied on a sounding rocket flight. An automated pre-programmed instrument permitted the injection of fluorescent labelled concanavalin A (Con A), culture medium and/or fixative at given times. An in-flight 1 g centrifuge allowed the comparison of the data obtained in microgravity with a 1 g control having the same history related to launch and re-entry. After flight, the cells fixed either at the onset of microgravity or after a or 12 minute incubation time with fluorescent concanavalin A were labelled for vimentin and actin and analysed by fluorescence microscopy. Binding of Con A to Jurkat cells is not influenced by microgravity, whereas patching of the Con A receptors is significantly lower. A significant higher number of cells show changes in the structure of vimentin in microgravity. Most evident is the appearance of large bundles, significantly increased in the microgravity samples. No changes are found in the structure of actin and in the colocalisation of actin on the inner side of the cell membrane with the Con A receptors after binding of the mitogen.     

双轴模拟式太阳敏感器测试链误差分析及修正

基于四象限硅光电池,双轴模拟式太阳敏感器（简称双轴模太）可实现太阳矢量两轴方向角的同时测量。硅光电池各象限光生电流的采集精度直接决定了敏感器的工作性能。然而,测试链环节中存在的不一致性误差会引起双轴模太角度测量偏差。为此,基于数值仿真,分析并建立了硅光电池各象限响应率、各象限光生电流的电压转换、信号放大、A/D转换系数及暗电流等环节偏差的影响模型,进而对各环节的综合偏差进行标定和修正,从而在保证敏感器测量精度的同时,降低对测试链环节的要求。测试结果表明,在不改变测试链性能的情况下,双轴模太的测量精度由修正前的2.05° (α轴,3σ)和1.94° (β轴,3σ)提高到0.28° (α轴,3σ)和0.26° (β轴,3σ)。     

Radiation protection against early and late effects of ionizing irradiation by the prostaglandin inhibitor indomethacin.

Protective effects of indomethacin, a prototype prostaglandin-inhibiting agent, against early and late sequelae of radiation injury (after X-rays or gamma rays) in mice were investigated. The following tissues or organs were examined: hematopoietic tissue, esophagus, jejunum, colon, lung, hair follicles, and tissues involved in the development of radiation-induced leg contractures. In addition, the effect of indomethacin was tested against radiation-induced carcinogenesis. In all experiments, the radiation was delivered as a single dose. Indomethacin led to significant protection of hematopoietic tissue, by a factor of 1.3. There was also some protection against radiation-induced pneumonitis and against radiation-induced carcinogenesis (protection factor of 1.2). The other tissues tested showed no change in their radioresponse after being treated with indomethacin. Thus, indomethacin can act as a radioprotective agent against both early and late sequelae of radiation, but its effect is dependent on the tissue tested. This protection is smaller than that observed with WR-2721. However, indomethacin combined with WR-2721 produced a radioprotective effect greater than the radioprotection achieved by individual treatments.     

Chromosomal changes in cultured human epithelial cells transformed by low- and high-LET radiation.

For a better assessment of radiation risk in space, an understanding of the responses of human cells, especially the epithelial cells, to low- and high-LET radiation is essential. In our laboratory, we have successfully developed techniques to study the neoplastic transformation of two human epithelial cell systems by ionizing radiation. These cell systems are human mammary epithelial cells (H184B5) and human epidermal keratinocytes (HEK). Both cell lines are immortal, anchorage dependent for growth, and nontumorigenic in athymic nude mice. Neoplastic transformation was achieved by irradiating cells successively. Our results showed that radiogenic cell transformation is a multistep process and that a single exposure of ionizing radiation can cause only one step of transformation. It requires, therefore, multihits to make human epithelial cells fully tumorigenic. Using a simple karyotyping method, we did chromosome analysis with cells cloned at various stages of transformation. We found no consistent large terminal deletion of chromosomes in radiation-induced transformants. Some changes of total number of chromosomes, however, were observed in the transformed cells. These transformants provide an unique opportunity for further genetic studies at a molecular level.     

Radiobiology and photobiology on Earth and in space: points of encounter and protection considerations.

All radiations originate in space, and the spectrum of radiations reaching the troposphere is limited only because of their range and absorption by the ozone layer above the atmosphere. Ultraviolet-C and the very heavy ions are therefore produced on earth only artificially, by special lamps and in accelerators. The range of biological effects of the different UV radiations and low and high LET radiations have been studied extensively, yet only recently new facts such as the production of DNA strand breaks by long wave UV light were established, adding to the various points of encounter existing between ionizing and nonionizing radiations. There are some similarities in radiation products, and the resulting effects of insult by radiation on biological systems very often are similar, if not the same. A common phenomenon that exists in all healthy biological cells is the ability to repair damage to DNA and thus either survive or mutate, and although the specific mechanisms of repair are somewhat different, the end result is the same. Recently a mechanism of improved radioprotection was found to involve an effect of certain radioprotective compounds on DNA repair. It is suggested that improved, and nontoxic, modes of protection may be offered by employing such compounds as biological response modifiers and natural substances. Further research is needed and is under way.     

Thin film bioreactors in space.

Studies from the Skylab, SL-3 and D-1 missions have demonstrated that biological organisms grown in microgravity have changes in basic cellular functions such as DNA, mRNA and protein synthesis, cytoskeleton synthesis, glucose utilization and cellular differentiation. Since microgravity could affect prokaryotic and eukaryotic cells at a subcellular and molecular level, space offers us an opportunity to learn more about basic biological systems with one important variable removed. The thin film bioreactor will facilitate the handling of fluids in microgravity, under constant temperature and will allow multiple samples of cells to be grown with variable conditions. Studies on cell cultures grown in microgravity would enable us to identify and quantify changes in basic biological function in microgravity which are needed to develop new applications of orbital research and future biotechnology.     

Coronal mass ejection speeds measured in the solar corona using LASCO C2 and C3 images

In this work we present height-time diagrams of 2 halo coronal mass ejections, observed on September 28th, 1997 and June 29th, 1999. The CMEs were observed by the Large Angle and Spectroscopic Coronagraph (LASCO), which observes the solar corona from 2 to 32 solar radii. To obtain these diagrams we divide the LASCO images of a given sequence in angular slices, transform them into rectangular slices (their width chosen proportional to the time distance to the next image) and place them side by side. Thus, the speed profile of any pattern moving in the particular latitudinal slice can be derived. With this method we were able to identify even minor speed changes in several angular positions for the chosen events. This technique is particularly appropriate to identify acceleration or deceleration of structures in halo CMEs.     

忆阻器的电路实现及其混沌动力学研究

提出一种新的忆阻器电路实现方法。该方法从忆阻器的定义出发,构造出忆阻器内部的基本变量磁通,利用光控电阻实现了磁通量对光电池的隔离控制,最终实现了一个可以独立作为二端口器件工作于电路及仿真中的忆阻器电路。给出了其电路实验结果,并将符合该忆阻器特性的数学模型用于蔡氏电路中替代其中的非线性二极管,研究此状况下产生混沌现象的可行性,为HP忆阻器今后应用于混沌电路提供可行性依据.     

Gravity and the cells of gravity receptors in mammals.

Two new findings, that crystals located in the inner ear gravity receptors of mammals have the internal organization requisite for the piezoelectric property, and that sensory hair cells of these same receptors possess contractile-appearing striated organelles, have prompted the author to model mammalian gravity receptors in the ear on the principles of piezoelectricity and bioenergetics. This model is presented and a brief discussion of its implications for the possible effects of weightlessness follows.     

Size and cell number of the utricle in kinetotically swimming fish: a parabolic aircraft flight study.

Humans taking part in parabolic aircraft flights (PAFs) may suffer from space motion sickness (SMS, a kinetosis). Since it has been repeatedly shown earlier that some fish of a given batch also reveal a kinetotic behavior during PAFs (especially so-called spinning movements and looping responses) and due to the homology of the vestibular apparatus among all vertebrates, fish can be used as model systems to investigate the origin of susceptibility to motion sickness. Therefore, we examined the utricular maculae (they are responsible for the internalization of gravity in teleosteans) of fish swimming kinetotically at microgravity in comparison with animals from the same batch who swam normally. On the histological level, it was found that the total number of both sensory and supporting cells of the utricular maculae did not differ between kinetotic animals as compared to normally swimming fish. Cell density (sensory and supporting cells/100 micrometers2), however, was reduced in kinetotic animals (p<0.0001), which seemed to be due to malformed epithelial cells (increase in cell size) of the kinetotic specimens. Susceptibility to kinetoses may therefore originate in malformed sensory epithelia.     

三维充液航天器的位置和姿态联合控制

考虑充液月球着陆器悬停避障阶段的控制问题,采用三维球摆作为液体晃动部分的等效模型.针对球摆与刚体耦合的三维动力学模型,给出动力学模型的矢量方程及各矢量在本体系的投影坐标,设计位置和姿态联合控制器.所设计的控制器可以稳定航天器刚体的位置和姿态,且只依赖刚体的位置和姿态,不依赖晃动角或者动力学方程,利于工程应用.利用LaSalle不变原理分析闭环系统的稳定性,给出期望姿态为竖直时系统渐近稳定的控制器参数选择依据.最后数值仿真验证控制方法的有效性.     

Tissue responses to low protracted doses of high LET radiations or photons: early and late damage relevant to radio-protective countermeasures.

Early and late murine tissue responses to single or fractionated low doses of heavy charged particles, fission-spectrum neutrons or gamma rays are considered. Damage to the hematopoietic system is emphasized, but results on acute lethality, host response to challenge with transplanted leukemia cells and life-shortening are presented. Low dose rates per fraction were used in some neutron experiments. Split-dose lethality studies (LD 50/30) with fission neutrons indicated greater accumulation of injury during a 9 fraction course (over 17 days) than was the case for gamma-radiation. When total doses of 96 or 247 cGy of neutrons or gamma rays were given as a single dose or in 9 fractions, a significant sparing effect on femur CFU-S depression was observed for both radiation qualities during the first 11 days, but there was not an earlier return to normal with dose fractionation. During the 9 fraction sequence, a significant sparing effect of low dose rate on CFU-S depression was observed in both neutron and gamma-irradiated mice. CFU-S content at the end of the fractionation sequence did not correlate with measured LD 50/30. Sustained depression of femur and spleen CFU-S and a significant thrombocytopenia were observed when a total neutron dose of 240 cGy was given in 72 fractions over 24 weeks at low dose rates. The temporal aspects of CFU-S repopulation were different after a single versus fractionated neutron doses. The sustained reduction in the size of the CFU-S population was accompanied by an increase in the fraction in DNA synthesis. The proliferation characteristics and effects of age were different for radial CFU-S population closely associated with bone, compared with the axial population that can be readily aspirated from the femur. In aged irradiated animals, the CFU-S proliferation/redistribution response to typhoid vaccine showed both an age and radiation effect. After high single doses of neutrons or gamma rays, a significant age- and radiation-related deficiency in host defense mechanisms was detected by a shorter mean survival time following challenge with transplantable leukemia cells. Comparison of dose-response curves for life shortening after irradiation with fission-spectrum neutrons or high energy silicon particles indicated high initial slopes for both radiation qualities at low doses, but for higher doses of silicon, the effect per Gy decreased to a value similar to that for gamma rays. The two component life-shortening curve for silicon particles has implications for the potential efficacy of radioprotectants. Recent studies on protection against early and late effects by aminothiols, prostaglandins, and other compounds are discussed.     

Microgravity and bone cell mechanosensitivity.

The capacity of bone tissue to alter its mass and structure in response to mechanical demands has long been recognized but the cellular mechanisms involved remained poorly understood. Bone not only develops as a structure designed specifically for mechanical tasks, but it can adapt during life toward more efficient mechanical performance. Mechanical adaptation of bone is a cellular process and needs a biological system that senses the mechanical loading. The loading information must then be communicated to the effector cells that form new bone or destroy old bone. The in vivo operating cell stress derived from bone loading is likely the flow of interstitial fluid along the surface of osteocytes and lining cells. The response of bone cells in culture to fluid flow includes prostaglandin (PG) synthesis and expression of prostaglandin G/H synthase inducible cyclooxygenase (COX-2). Cultured bone cells also rapidly produce nitric oxide (NO) in response to fluid flow as a result of activation of endothelial nitric oxide synthase (ecNOS), which enzyme also mediates the adaptive response of bone tissue to mechanical loading. Earlier studies have shown that the disruption of the actin-cytoskeleton abolishes the response to stress, suggesting that the cytoskeleton is involved in cellular mechanotransduction. Microgravity, or better near weightlessness, is associated with the loss of bone in astronauts, and has catabolic effects on mineral metabolism in bone organ cultures. This might be explained as resulting from an exceptional form of disuse under near weightlessness conditions. However, under near weightlessness conditions the assembly of cytoskeletal elements may be altered since it has been shown that the direction of the gravity vector determines microtubular pattern formation in vivo. We found earlier that the transduction of mechanical signals in bone cells also involves the cytoskeleton and is related to PGE2 production. Therefore it is possible that the mechanosensitivity of bone cells is altered under near weightlessness conditions, and that this abnormal mechanosensation contributes to disturbed bone metabolism observed in astronauts. In our current project for the International Space Station, we wish to test this hypothesis experimentally using an in vitro model. The specific aim of our research project is to test whether near weightlessness decreases the sensitivity of bone cells for mechanical stress through a decrease in early signaling molecules (NO, PGs) that are involved in the mechanical loading-induced osteogenic response. Bone cells are cultured with or without gravity prior to and during mechanical loading, using our modified in vitro oscillating fluid flow apparatus. In this "FlowSpace" project we are developing a cell culture module that is used to provide further insight in the mechanism of mechanotransduction in bone.