Abstract: The study of the microbial control technology for the sealed cabin of manned spacecraft aims to guide the microbial control work in the subsequent long-term residence stage. In this paper, with a short- and middle-term resident manned spacecraft as an example, the key issues of the microbial control of the spacecraft in the ground development stage before launch are discussed, and the related control methods are proposed. The actual detection of the microbial control situation within the sealed cabin shows that the microbial control level can meet the requirements for the long-term stay in the orbit, and the control methods are effective.