Abstract: Rerieving atmospheric ozone using 1.27μm twilight airglow is an important method of mesospheric atmospheric ozone measurements. In this paper, the information content,measurable height and venical resolution have been studied, and the effect of the uncertainty of photocheemical and atmospheric models parameters and the sensitivity of the variation in mesospheric ozone content have been discussed. The numerical simulation results show that the atmospheric ozone content in 60-9Okm altitude may be given using 1.27μm twilight airglow measurements, the effect of the uncertainty of photochemical model parameters is about 1O%.